Summary of RB50
Page 1
Rb50 microscope user’s manual info@feinoptic.Com.
Page 2
Info@feinoptic.Com page 1 microscope components: beam splitter trinocular port eyepieces mechanical stage condenser y-axis knob x-axis knob hex wrench voltage indicator reset button for intensity light rheostat adjustment knob coarse focusing fine focusing focusing tension adjustment illuminator fie...
Page 3
Info@feinoptic.Com page 2 microscope components: rb50 fluorescence microscope filter levers coarse adjustment limit ring coarse focus knob fine focus knob nd filter slots quintuple nosepiece fluorescence illuminator attachment 100w halogen light source.
Page 4
Binocular / trinocular microscope assembly: info@feinoptic.Com page 3 rb50 microscope assembly rb50 fluorescence microscope assembly.
Page 5
Before use: when moving the microscope use both hands, holding by the handle at the back (1) and the base (2) as shown at right. Do not shake or drop the microscope. Do not not expose the microscope to direct sun, high temperatures, dust, or damp environments. Use a flat work surface. Indoor operati...
Page 6
Maintenance: info@feinoptic.Com page 5 if the microscope becomes wet during use, power off the microscope and dry the microscope thoroughly. Wipe lenses gently with a soft tissue. Carefully remove excess oil from the 100x immersion oil lens. Wipe off fingerprints from lens surfaces with lens paper u...
Page 7
Step-by-step assembly: info@feinoptic.Com page 6 light source assembly & bulb replacement: loosen the lock screw (1) completely with an m4 spanner and remove the cover. Open the bulb lock (2). Handle the bulb (3) with a clean glove or soft tissue (do not touch the bulb with fingers). Insert the bulb...
Page 8
Step-by-step assembly: info@feinoptic.Com page 7 condenser assembly: rotate the coarse focusing knob (1) to raise the stage to its highest setting. Rotate the condenser height adjustment knob (2) to lower the condenser bracket to its lowest setting. Loosen the condenser set screw (3). Swing out the ...
Page 9
Step-by-step assembly: info@feinoptic.Com page 8 eyepiece assembly: remove the cover from the eyetube (1). Insert the eyepiece into the eyetube. Match up the positioning screw (2) into the eyetube groove (3) when inserting the eyepiece into the eyetube. Power cord connection: set the main switch to ...
Page 10
Step-by-step assembly: info@feinoptic.Com page 9 slide placement: push the slide holder clamp (1) backwards. Place the slide between the slide holder clamp (1) and the slide holder (2) with the cover slip facing up. Rotate the x-axis knob (4) and the y-axis knob (3) to position the slide in the cent...
Page 11
Step-by-step assembly: info@feinoptic.Com page 10 focus tension adjustment: if the focus knob is very tight or the stage drifts after being focused, the tension adjustment knob (1) should be adjusted. Rotating the knob clockwise will tighten the tension and rotating it counterclockwise will loosen t...
Page 12
Step-by-step assembly: info@feinoptic.Com page 11 stage adjustment: when looking through the microscope, move the stage by rotating the x-axis adjustment knob (1) and the y-axis adjustment knob (2). The movement range of the x-axis and y-axis is 80 x 55mm. X-axis & y-axis knob adjustment: hold the x...
Page 13
Step-by-step assembly: info@feinoptic.Com page 12 centering the condenser: rotate the condenser raise/lower knob (1) to raise the condenser up to the highest position. Rotate the spanner (2) on the condenser to move the front lens into the light path. Use this condenser lens in the light path when t...
Page 14
Step-by-step assembly: info@feinoptic.Com page 13 using color filters: a color filter can make the background light more suitable and strengthen the image contrast. When an external color filter is used, place a 45mm diameter filter into the groove on top of the illuminator (1). Place the rough side...
Page 15
Fluorescence assembly: info@feinoptic.Com page 14 fluorescence illuminator assembly: remove the caps (1) from the illuminator. Place the illuminator on the microscope body then slide it toward the light source to make the epi-illuminator flat with the body. Tighten the four m5 hex screws in the illu...
Page 16
Fluorescence assembly: info@feinoptic.Com page 15 nosepiece assembly: loosen the set screw on the arm (1). Match the dovetail interface (2) of the nosepiece with the dovetail groove of the arm. Push the nosepiece into the slot. Tighten the set screw (1). Fluorescence light source assembly: loosen th...
Page 17
Fluorescence assembly: info@feinoptic.Com page 16 centering the field diaphragm: by adjusting the field diaphragm, the beam of light is adjusted according to the objective and will create sharper image contrast. To prevent a fluorescence decrease, narrow the field diaphragm and reduce the illuminate...
Page 18
Fluorescence assembly: info@feinoptic.Com page 17 centering the aperture diaphragm: rotate the turntable to place the fluorescence filter b or g into the light path. (if there is no fluorescence filter b or g use another). Rotate the 10x objective into the light path. Put the shutter (1) to position...
Page 19
Fluorescence assembly: info@feinoptic.Com page 18 installing filters: image contrast and background light can be adjusted by using filters. Insert desired filter or nd filter into position (1) and position (2) on the side of the microscope. The filter idenfication side of the filter should face the ...
Page 20
Troubleshooting: info@feinoptic.Com page 19 optical troubleshooting problem cause solution led light is bright, but field of view is dark. Field diaphragm is not large enough. Condenser is too low. Condenser is not centered. Open the field diaphragm. Adjust the condenser. Center the condenser. Side ...
Page 21
Troubleshooting: info@feinoptic.Com page 20 mechanical & electrical troubleshooting problem cause solution can not get the objective lens to focus. The cover glass is not facing up. The cover glass is not standard thickness. Put the cover glass face up. Objective touches the cover glass when rotatin...