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Hach DR2400 Manual

Summary of DR2400

  • Page 1

    59400-22 dr/2400 spectrophotometer procedure manual © hach company, 2004. All rights reserved. Printed in the u.S.A. Te/dk 3/04 1ed.

  • Page 2: Hach Company Trademarks

    5940022title.Fm page 2 of 2 hach company trademarks accugrow ® accuvac ® accuver™ accuvial™ add-a-test™ agritrak™ aluver ® amver™ apa 6000™ aquachek™ aquatrend ® bariver ® bodtrak™ borotrace™ borover ® c. Moore green™ ca 610™ calver ® chromaver ® colorquik ® cooltrak ® cuver ® cyaniver ® digesdahl ®...

  • Page 3: Table of Contents

    Table of contents 5940022toc.Fm page 3 table of contents hach company trademarks ..................................................................................................................2 section 1 introduction ...................................................................................

  • Page 4: Table of Contents

    Table of contents table of contents page 4 5940022toc.Fm 3.4.2 method detection limit (mdl) ................................................................................................................42 3.4.3 precision ................................................................................

  • Page 5: Table of Contents

    Table of contents (denotes usepa accepted or approved for water or wastewater analysis. Table of contents 5940022toc.Fm page 5 4.7.1 how to obtain an msds............................................................................................................................60 4.7.2 sections of a...

  • Page 6: Table of Contents

    Table of contents (denotes usepa accepted or approved for water or wastewater analysis. Table of contents page 6 5940022toc.Fm chlorine dioxide amaranth method (20 to 500 µg/l) chlorine dioxide method 8065 chlorophenol red method lr (0.01 to 1.00 mg/l) chlorine, free ✭method 8021 dpd method powder p...

  • Page 7: Table of Contents

    Table of contents (denotes usepa accepted or approved for water or wastewater analysis. Table of contents 5940022toc.Fm page 7 copper bathocuproine method unicell™ vials (0.10 to 6.00 mg/l) cyanide method 8027 pyridine-pyrazalone method powder pillows (0.001 to 0.240 mg/l cn – ) cyanide cyanogen chl...

  • Page 8: Table of Contents

    Table of contents (denotes usepa accepted or approved for water or wastewater analysis. Table of contents page 8 5940022toc.Fm lead par method unicell™ vials (0.20 to 2.00 mg/l) manganese ✭method 8034 periodate oxidation method powder pillows hr (0.2 to 20.0 mg/l) manganese method 8149 1-(2-pyridyla...

  • Page 9: Table of Contents

    Table of contents (denotes usepa accepted or approved for water or wastewater analysis. Table of contents 5940022toc.Fm page 9 powder pillows (0.01 to 0.50 mg/l nh 3 –n) nitrogen, ammonia method 10031 salicylate method test ‘n tube ™ vials hr (0.4 to 50.0 mg/l nh 3 –n) nitrogen, ammonia method 10023...

  • Page 10: Table of Contents

    Table of contents (denotes usepa accepted or approved for water or wastewater analysis. Table of contents page 10 5940022toc.Fm 3 to 450 µg/l deha; 9 to 1000 µg/l hydroquinone; 13 to 1500 µg/l iso-ascorbic acid [isa]; 15 to 1000 µg/l methylethyl ketoxime [meko] ozone method 8311 indigo method accuva...

  • Page 11: Table of Contents

    Table of contents (denotes usepa accepted or approved for water or wastewater analysis. Table of contents 5940022toc.Fm page 11 test ‘n tube™ vials hr (1.0 to 100.0 mg/l po 4 3– ) phosphorus, total ascorbic acid method with acid persulfate digestion unicell™ vials (1.5 to 15.0 mg/l po 4 3– ) phospho...

  • Page 12

    Table of contents page 12 5940022toc.Fm volatile acids method 8196 esterification method (27 to 2800 mg/l) zinc ✭method 8009 zincon method powder pillows (0.01 to 2.00 mg/l) zinc par unicell™ vials (0.10 to 6.00 mg/l zn).

  • Page 13: Section 1

    Introduction 5940022intro.Fm page 13 section 1 introduction 1.1 abbreviations used in this manual the following abbreviations are used throughout the text of the procedure section: table 1 abbreviation definition abbreviation definition °c degree(s) celsius (centigrade) hr high range °f degree(s) fa...

  • Page 14: Introduction

    Introduction introduction page 14 5940022intro.Fm 1.2 conversions 1.2.1 chemical species 1.2.2 hardness table 2 lists the factors for converting hardness from one unit of measure to another. For example, to convert mg/l caco 3 to german parts/100,000 cao, multiply the value in mg/l x 0.056 . Table 2...

  • Page 15: Section 2

    Laboratory practices 5940022labprac.Fm page 15 section 2 laboratory practices 2.1 temperature most tests in this manual perform most accurately when the sample temperature is between 20 °c and 25 °c (68 to 77 °f). A note in the individual procedure will state any special temperature requirements. 2....

  • Page 16: Laboratory Practices

    Laboratory practices laboratory practices page 16 5940022labprac.Fm the hach digesdahl system is a process that yields a digest suitable for the determination of metals, total phosphorus and total kjeldahl nitrogen (tkn). It is rapid, convenient, and the method of choice. For usepa reporting purpose...

  • Page 17: Laboratory Practices

    Laboratory practices laboratory practices 5940022labprac.Fm page 17 7. Warm the beaker. Add 5 ml of 5.0 n sodium hydroxide and quantitatively transfer the sample with deionized water to a volumetric flask. See table 3 below for the suggested final volume. 8. Adjust the sample to ph 4 by drop-wise ad...

  • Page 18: Laboratory Practices

    Laboratory practices laboratory practices page 18 5940022labprac.Fm figure 2 general purpose distillation apparatus 2.5 filtration filtration separates particulates from an aqueous sample. It uses a porous medium that retains particulates but allows liquids to pass through. It is useful for removing...

  • Page 19: Laboratory Practices

    Laboratory practices laboratory practices 5940022labprac.Fm page 19 figure 3 vacuum filtration required apparatus for vacuum filtration description unit cat. No. Filter discs, glass fiber, 47-mm ......................................................................................... 100/pkg...........

  • Page 20: Laboratory Practices

    Laboratory practices laboratory practices page 20 5940022labprac.Fm figure 4 gravity filtration required apparatus for gravity filtration description unit cat no. Cylinder, graduated, 100-ml ............................................................................................ Each...............

  • Page 21: Laboratory Practices

    Laboratory practices laboratory practices 5940022labprac.Fm page 21 term reagent blank refers to that portion of the test result contributed solely by the reagent. This produces a positive error in the test results. Every effort is made to produce reagents with the lowest possible blank; and, for mo...

  • Page 22: Laboratory Practices

    Laboratory practices laboratory practices page 22 5940022labprac.Fm more accurate dilutions can be done with a pipet and a 100-ml volumetric flask (see table 5 for more information). Pipet the sample and dilute to volume with deionized water. Swirl to mix. 2.7.1 sample dilution with interfering subs...

  • Page 23: Laboratory Practices

    Laboratory practices laboratory practices 5940022labprac.Fm page 23 3. Invert the ampule several times to dissolve the reagent. Do not place your finger over the broken end; the liquid will stay in the ampule when inverted. Wipe the ampule with a lint-free cloth to remove fingerprints, etc. 4. Inser...

  • Page 24: Laboratory Practices

    Laboratory practices laboratory practices page 24 5940022labprac.Fm 2.10.3 cleaning of sample cells most laboratory detergents can be used at recommended concentrations. Neutral detergents, such as liquinox, are safer when regular cleaning is required. When using a detergent, you can clean faster by...

  • Page 25: Laboratory Practices

    Laboratory practices laboratory practices 5940022labprac.Fm page 25 2.12 achieving accuracy in volume measurement 2.12.1 pipets and graduated cylinders when smaller sample quantities are used, the accuracy of measurements becomes increasingly important. Figure 7 illustrates the proper way to read th...

  • Page 26: Laboratory Practices

    Laboratory practices laboratory practices page 26 5940022labprac.Fm the pour-thru cell can be used with the following hach chemistries, but special care must be taken to rinse the cell with deionized water between samples. The pour-thru cell can also be used to run nitrogen, ammonia, nessler method,...

  • Page 27: Section 3

    Chemical analysis 5940022chemanal.Fm page 27 section 3 chemical analysis 3.1 sample collection, preservation, and storage correct sampling and storage are critical for accurate testing. Sampling devices and containers must be thoroughly clean to prevent carryover from previous samples. Preserve the ...

  • Page 28: Chemical Analysis

    Chemical analysis chemical analysis page 28 5940022chemanal.Fm d. Rinse well with deionized water. For chromium, 12–15 rinses may be necessary. When determining ammonia and kjeldahl nitrogen, the rinse water must be ammonia-free. E. Air dry. Protect the glassware from fumes and other sources of cont...

  • Page 29: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 29 3.1.3 correcting for volume additions if you use a large volume of preservative or neutralizer, you must account for dilution by the acid added to preserve the sample, and/or the base used to adjust the ph to the range of the procedure. ...

  • Page 30: Chemical Analysis

    Chemical analysis chemical analysis page 30 5940022chemanal.Fm 27. Hardness p, g hno 3 to ph 2 so 4 to ph 6 months 28. Hydrogen ion (ph) p, g none required analyze immediately 31, 43. Kjeldahl and organic nitrogen p, g cool 4 °c, h 2 so 4 to ph 28 days metals 8 18. Chromium vi p, g cool, 4 °c 24 hou...

  • Page 31: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 31 3.2 checking for accuracy and precision accuracy defines the closeness of a test result to the true value. Precision defines the closeness of repeated measurements to each other. Although precise results suggest accuracy, they can be ina...

  • Page 32: Chemical Analysis

    Chemical analysis chemical analysis page 32 5940022chemanal.Fm 3.2.2 standard additions in general, this technique requires adding a small amount of a standard solution to the sample water and repeating the procedure. You should get close to 100% recovery. If not, a problem exists and must be identi...

  • Page 33: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 33 figure 9 standard additions decision tree branch b perform multiple standard additions on a sample of deionized water as follows: note: for this example, a 25 ml sample size and a 50 mg/l iron standard is used. 1. Pour 25 ml of deionized...

  • Page 34: Chemical Analysis

    Chemical analysis chemical analysis page 34 5940022chemanal.Fm if the results using deionized water give the expected increments (as they did in the example above), go to branch c. If the results using deionized water do not give the expected increments, the problem may lie with another part of the ...

  • Page 35: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 35 example a example b figure 10 multiple standard additions — a and b the plot for example a illustrates an interference that becomes progressively worse as the concentration of the standard or analyte increases. This type of interference ...

  • Page 36: Chemical Analysis

    Chemical analysis chemical analysis page 36 5940022chemanal.Fm example c example d figure 11 multiple standard additions — c and d the plot for example c illustrates a common interference with a uniform effect on the standard and the substances in the sample. The four data points form a straight lin...

  • Page 37: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 37 apparent interferences may also be caused by a defect in the instrument or standards. Before assuming the interference is chemical, check branch b. Branch h check the operation of the instrument and/or apparatus used to perform the test....

  • Page 38: Chemical Analysis

    Chemical analysis chemical analysis page 38 5940022chemanal.Fm f. Verify the calibration curve adjustment (standard adjust) currently in use. The factory-stored default calibration should be used initially to check the standard. G. Verify that the dilution factor option is correct. If the instrument...

  • Page 39: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 39 because it may not be feasible to determine the cause of the interference, diluting the sample past the point of interference is often the most economical and efficient means of getting the correct result. If it is not possible to dilute...

  • Page 40: Chemical Analysis

    Chemical analysis chemical analysis page 40 5940022chemanal.Fm example: a sample measures 10 mg/l. A separate 100-ml portion of the sample was spiked with 5 ml of a 100-mg/l standard solution. The spiked solution was measured by the same method as the original sample. The result was 13.7 mg/l. Accep...

  • Page 41: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 41 3.3 interferences interferences are contaminants in a sample that are capable of causing changes in color development, turbidity, or unusual colors and odors, thereby creating errors in your results. A list of common interferences is inc...

  • Page 42: Chemical Analysis

    Chemical analysis chemical analysis page 42 5940022chemanal.Fm 8. Some purchased standards may be very acidic and will not work directly with hach procedures. Adjust the ph of these standards as described above. Adjust the final concentration of the standard for the dilution. The hach standard solut...

  • Page 43: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 43 3. Analyze at least seven portions of the laboratory standard and record each result. 4. Calculate the average and the standard deviation(s) of the results. 5. Compute the mdl using the appropriate student’s t value (see table below) and...

  • Page 44: Chemical Analysis

    Chemical analysis chemical analysis page 44 5940022chemanal.Fm each standard and use the corrected standard values to calculate the average and standard deviation used in the mdl. 3.4.3 precision every measurement has some degree of uncertainty. Just as a ruler with markings of 1 mm leaves some doub...

  • Page 45: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 45 use sensitivity when comparing different methods. For example, hach has three dr/2400 methods for determining iron: notice that the ferrozine method has the greatest sensitivity of the three methods because it will measure the smallest c...

  • Page 46: Chemical Analysis

    Chemical analysis chemical analysis page 46 5940022chemanal.Fm figure 12 semilogarithmic calibration curve to convert %t readings to concentration, prepare a table such as table 9 and select the appropriate line from the “%t tens” column and the appropriate column from the “% t units” columns. The %...

  • Page 47: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 47 3.5.2 absorbance versus concentration calibration if absorbance values are measured, plot the results on linear graph paper. Plot the absorbance value on the vertical axis and the concentration on the horizontal axis. Plot increasing abs...

  • Page 48: Chemical Analysis

    Chemical analysis chemical analysis page 48 5940022chemanal.Fm 9. Adjust the wavelength to 50 nm more than the highest absorbance point on the initial search (step 8). Re-zero as in step 5. 10. Measure and record the absorbance. Repeat, decreasing the absorbance in 5-nm steps. Re-zero, then measure ...

  • Page 49: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 49 figure 13 selecting the best wavelength adapting a buret titration for use with a digital titrator any standard titration procedure that uses a buret can be adapted to the digital titrator by using the following procedure. 1. From table ...

  • Page 50: Chemical Analysis

    Chemical analysis chemical analysis page 50 5940022chemanal.Fm 3. When using the digital titrator for your buret method, note the number of digits required for a sample titration. To convert the digits required to the equivalent number of milliliters for a buret method, calculate: if the sample size...

  • Page 51: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 51 pao, phenylarsine oxide, 0.0451 n 21420-01 potassium dichromate, 1.00 n 21971-01 silver nitrate, 0.2256 n 14396-01 silver nitrate, 1.128 n 14397-01 sodium hydroxide, 0.1600 n 14377-01 sodium hydroxide, 0.1612 n 24280-02 sodium hydroxide,...

  • Page 52: Chemical Analysis

    Chemical analysis chemical analysis page 52 5940022chemanal.Fm 3.7 comparison of international drinking water guidelines table 13 comparison of international drinking water and fda bottled water guidelines 1 parameter usepa 2 maximum contaminant level (mcl) canada 3 maximum acceptable concentration ...

  • Page 53: Chemical Analysis

    Chemical analysis chemical analysis 5940022chemanal.Fm page 53 3.7.1 definitions of usepa approved and accepted usepa approved the united states environmental protection agency (usepa) establishes limits for maximum contamination levels of certain constituents in water. It also requires that specifi...

  • Page 54: Chemical Analysis

    Chemical analysis chemical analysis page 54 5940022chemanal.Fm • american public health association • american water works association • water environmental federation • american society for testing and materials • united states geological survey • association of official analytical chemists when a ...

  • Page 55: Section 4

    Waste management and safety 5940022wasteandsafety.Fm page 55 section 4 waste management and safety this section provides guidelines for laboratory waste management. These guidelines are only a summary of basic usepa requirements, and do not relieve the user from complying with the complete regulatio...

  • Page 56: Waste Management and Safety

    Waste management and safety waste management and safety page 56 5940022wasteandsafety.Fm • it is a discarded commercial chemical product, off-specification species, container residue, or spill residue of materials specifically listed in 40 cfr 261.33 (p- and u-codes); • it is a waste from a specific...

  • Page 57: Waste Management and Safety

    Waste management and safety waste management and safety 5940022wasteandsafety.Fm page 57 • flash point is below 60 °c (140 °f), or it is classified by dot as an oxidizer (d001). • the ph of the material is ≤2 or ≥12.5 (d002). • the material is unstable, reacts violently with water, may generate toxi...

  • Page 58: Waste Management and Safety

    Waste management and safety waste management and safety page 58 5940022wasteandsafety.Fm 4.4 management of specific wastes hach has several documents to assist customers in managing waste that has been generated from our products. You can obtain the following documents by calling 1-800-227-4224 or 9...

  • Page 59: Waste Management and Safety

    Waste management and safety waste management and safety 5940022wasteandsafety.Fm page 59 3. Task force on laboratory waste management. Less is better; 2nd ed.; american chemical society, department of government relations and science policy: washington, dc 1993. 4. Committee on chemical safety. Safe...

  • Page 60: Waste Management and Safety

    Waste management and safety waste management and safety page 60 5940022wasteandsafety.Fm • gloves to protect skin from toxic or corrosive materials, sharp objects, very hot or very cold materials, or broken glass. Use tongs or finger cots when transferring hot apparatus. • laboratory coats or splash...

  • Page 61: Waste Management and Safety

    Waste management and safety waste management and safety 5940022wasteandsafety.Fm page 61 4.7.2 sections of an msds each msds has ten sections. The sections and the information found in them are described below. Header information the hach catalog number, msds date, change number, company address and...

  • Page 62: Waste Management and Safety

    Waste management and safety waste management and safety page 62 5940022wasteandsafety.Fm • lower flammable limit (lfl or lel): the lowest concentration that will produce a fire or flash when an ignition source is present. • upper flammable limit (ufl or uel): the vapor concentration in air above whi...

  • Page 63: Section 5

    Procedures explained 5940022proceduresexplained.Fm page 63 section 5 procedures explained dr/2400 sulfide sulfide_none_other_mlb_eng_ody.Fm page 1 of 4 • analyze samples immediately. Do not preserve for later analysis. • avoid excessive agitation of samples to minimize sulfide loss. • some sulfide l...

  • Page 64: Procedures Explained

    Procedures explained procedures explained page 64 5940022proceduresexplained.Fm sulfide sulfide sulfide_procedures explained_page 3.Fm page 3 of 4 interferences accuracy check sulfide standard solutions are very unstable and should be prepared from sodium sulfate and standardized as described in sta...

  • Page 65: Dr/2400

    Dr/2400 alachlor ala_none_other_imm_eng_ody.Fm page 1 of 8 this method analyzes for alachlor in water. Sample calibrators and reagents are added to cuvettes coated with alachlor-specific antibodies. The color that develops is then measured and compared with the color measurements of the calibrators....

  • Page 66: Alachlor

    Alachlor alachlor page 2 of 8 ala_none_other_imm_eng_ody.Fm 1. Touch single wavelength then touch the λ button. Type in 450 nm and touch ok. 2. Label an antibody cuvette for each calibrator and each sample to be tested. Note: as many as 20 cuvettes may be tested at one time and may comprise any comb...

  • Page 67: Alachlor

    Alachlor alachlor ala_none_other_imm_eng_ody.Fm page 3 of 8 9. At the end of the 20-minute period, discard the contents of all the cuvettes into an appropriate waste container. 10. Wash each cuvette forcefully and thoroughly four times with deionized water. Empty the rinse water into the waste conta...

  • Page 68: Alachlor

    Alachlor alachlor page 4 of 8 ala_none_other_imm_eng_ody.Fm measuring the color 15. Label and fill a zeroing cuvette with deionized water. Wipe the outside of all the cuvettes with a tissue to remove water, smudges, and fingerprints. 16. Install the 1-cm square cell adapter. Note: see section 2.6 sa...

  • Page 69: Alachlor

    Alachlor alachlor ala_none_other_imm_eng_ody.Fm page 5 of 8 using the 1-cm microcuvette rack this rack (see figure 14) has been designed specifically to aid in achieving precise and accurate results when using the immunoassay technique to analyze several samples at the same time. Figure 14 the 1-cm ...

  • Page 70: Alachlor

    Alachlor alachlor page 6 of 8 ala_none_other_imm_eng_ody.Fm interpretation sample #1 — sample reading is less than the readings for both calibrators. Therefore the sample concentration of alachlor is greater than both 0.1 ppb and 0.5 ppb alachlor. Sample #2 — sample reading is between the readings f...

  • Page 71: Alachlor

    Alachlor alachlor ala_none_other_imm_eng_ody.Fm page 7 of 8 diluting water samples other levels of alachlor can be tested by diluting the sample and comparing the results to the 0.1 ppb calibrator. Select the appropriate sample volume from the table below, place it in a graduated mixing cylinder, an...

  • Page 72: Alachlor

    Alachlor © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 8 of 8 ala_none_other_imm_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach offi...

  • Page 73: Dr/2400

    Dr/2400 aluminum aluminum_pp_other_aln_eng_ody.Fm page 1 of 6 • digestion is required for determining total aluminum. See section 4 on page 55 for the digestion procedure. • the sample temperature must be between 20–25 °c (68–77 °f) for accurate results. • clean all glassware with 6.0 n hcl and deio...

  • Page 74: Aluminum

    Aluminum aluminum page 2 of 6 aluminum_pp_other_aln_eng_ody.Fm 5. Touch the timer icon. Touch ok . Invert repeatedly for one minute to dissolve the powder. Undissolved powder will cause inconsistent results. 6. Pour 25 ml of the mixture into a 25-ml sample cell. (this is the prepared sample.) 7. Add...

  • Page 75: Aluminum

    Aluminum aluminum aluminum_pp_other_aln_eng_ody.Fm page 3 of 6 interferences fluoride interferes at all levels by complexing with aluminum. The actual aluminum concentration can be determined using the fluoride interference graph when the fluoride concentration is known. To use the fluoride interfer...

  • Page 76: Aluminum

    Aluminum aluminum page 4 of 6 aluminum_pp_other_aln_eng_ody.Fm figure 1 fluoride interference graph sample collection, storage and preservation collect samples in a clean glass or plastic containers. Preserve the sample by adjusting the ph to 2 or less with nitric acid (cat. No. 152-49) (about 1.5 m...

  • Page 77: Aluminum

    Aluminum aluminum aluminum_pp_other_aln_eng_ody.Fm page 5 of 6 standard solution method 1. Prepare a 0.4-mg/l aluminum standard solution as follows: pipet 1.00 ml of aluminum standard solution, 100-mg/l as al 3+ , into a 250-ml volumetric flask. 2. Dilute to the mark with deionized water. Prepare th...

  • Page 78: Aluminum

    Aluminum © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 aluminum_pp_other_aln_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach o...

  • Page 79: Dr/2400

    Dr/2400 aluminum aluminum_pp_other_ecr_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blan...

  • Page 80: Aluminum

    Aluminum aluminum page 2 of 6 aluminum_pp_other_ecr_eng_ody.Fm 5. Touch the timer icon. Touch ok. A 30-second reaction period will begin. 6. After 30 seconds add the contents of one hexamethylene- tetramine buffer reagent powder pillow for 20-ml sample size. 7. Stopper and invert to mix until the po...

  • Page 81: Aluminum

    Aluminum aluminum aluminum_pp_other_ecr_eng_ody.Fm page 3 of 6 interferences polyphosphate interference can be reduced by converting polyphosphate to orthophosphate by the following steps: 13. Touch zero . The display will show: 0.008 mg/l al 3+ this test uses a non-zero intercept for the calibratio...

  • Page 82: Aluminum

    Aluminum aluminum page 4 of 6 aluminum_pp_other_ecr_eng_ody.Fm a. Rinse a 50-ml graduated mixing cylinder and a 125-ml erlenmeyer flask containing a magnetic stir bar with 6 n hydrochloric acid (cat. No. 884-49). Rinse again with deionized water. This will remove any aluminum present. Note: rinse tw...

  • Page 83: Aluminum

    Aluminum aluminum aluminum_pp_other_ecr_eng_ody.Fm page 5 of 6 sample collection, storage and preservation collect samples in a clean glass or plastic containers. Preserve samples by adjusting the ph to 2 or less with concentrated nitric acid (about 1.5 ml per liter) (cat. No. 2540-49). Preserved sa...

  • Page 84: Aluminum

    Aluminum © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 aluminum_pp_other_ecr_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach o...

  • Page 85: Dr/2400

    Dr/2400 aluminum aluminum_unicell_other_chr_eng_ody.Fm page 1 of 4 • analyze collected samples in glass or plastic bottles as quickly as possible. • adjust ph of preserved samples to between ph 2–3 with 5.0 n sodium hydroxide standard solution before analysis. • clean the outside of the vial before ...

  • Page 86: Aluminum

    Aluminum aluminum page 2 of 4 aluminum_unicell_other_chr_eng_ody.Fm interference the ions listed in the table have been tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. 5. Cap the vial. Invert the sample until the solids dissolve comp...

  • Page 87: Aluminum

    Aluminum aluminum aluminum_unicell_other_chr_eng_ody.Fm page 3 of 4 higher concentrations of heavy metals than those given, as well as fluoride, phosphate and relatively rare elements such as beryllium, thorium, titanium, zirconium and vanadium interfere with the determination. Aluminum oxide hydrat...

  • Page 88: Aluminum

    Aluminum © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 aluminum_unicell_other_chr_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the h...

  • Page 89: Dr/2400

    Dr/2400 arsenic arsenic_none_other_sdc_eng_ody.Fm page 1 of 6 • this procedure requires a user-entered calibration for each new lot of arsenic absorber solution. See calibration on page 5. • some variations of the calibration procedure are possible. See the instrument manual for details. • wipe the ...

  • Page 90: Arsenic

    Arsenic arsenic page 2 of 6 arsenic_none_other_sdc_eng_ody.Fm 5. Prepare the arsenic absorber solution as directed under reagent preparation on page 4. 6. Using a graduated cylinder, pour 25-ml of prepared arsenic absorber solution into the cylinder/gas bubbler assembly. Attach it to the distillatio...

  • Page 91: Arsenic

    Arsenic arsenic arsenic_none_other_sdc_eng_ody.Fm page 3 of 6 interferences sample collection, storage and preservation collect samples in acid washed glass or plastic bottles. Adjust the ph to 2 or less with sulfuric acid (about 2 ml per liter) (cat. No. 1270-32). Preserved samples may be stored up...

  • Page 92: Arsenic

    Arsenic arsenic page 4 of 6 arsenic_none_other_sdc_eng_ody.Fm reagent preparation prepare the arsenic absorber solution as follows: 1. Weigh 1.00 g of silver diethyldithiocarbamate on an analytical balance. 2. Transfer the powder to a 200-ml volumetric flask. Dilute to volume with pyridine. You must...

  • Page 93: Arsenic

    Arsenic arsenic arsenic_none_other_sdc_eng_ody.Fm page 5 of 6 calibration standard preparation perform a new calibration for each lot of arsenic absorber solution. 1. Prepare a 10.0-mg/l arsenic working standard by pipetting 10.0 ml of arsenic standard solution, 1000 mg/l as (cat. No. 14571-42) into...

  • Page 94: Arsenic

    Arsenic © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 arsenic_none_other_sdc_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach o...

  • Page 95: Dr/2400

    Dr/2400 atrazine atz_none_other_imm_eng_ody.Fm page 1 of 8 this method analyzes for atrazine in water. Sample calibrators and reagents are added to cuvettes coated with atrazine-specific antibodies. The color that develops is then measured and compared with the color measurements of the calibrators....

  • Page 96: Atrazine

    Atrazine atrazine page 2 of 8 atz_none_other_imm_eng_ody.Fm 1. Touch single wavelength then touch the λ button. Type in 450 nm and touch ok. 2. Label an antibody cuvette for each calibrator and each sample to be tested. Note: as many as 20 cuvettes may be tested at one time and may comprise any comb...

  • Page 97: Atrazine

    Atrazine atrazine atz_none_other_imm_eng_ody.Fm page 3 of 8 9. At the end of the 20-minute period, discard the contents of all the cuvettes into an appropriate waste container. 10. Wash each cuvette forcefully and thoroughly four times with deionized water. Empty the rinse water into the waste conta...

  • Page 98: Atrazine

    Atrazine atrazine page 4 of 8 atz_none_other_imm_eng_ody.Fm measuring the color 15. Label and fill a zeroing cuvette with deionized water. Wipe the outside of all the cuvettes with a tissue to remove water, smudges, and fingerprints. 16. Install the 1-cm square cell adapter. Note: see section 2.6 sa...

  • Page 99: Atrazine

    Atrazine atrazine atz_none_other_imm_eng_ody.Fm page 5 of 8 using the 1-cm microcuvette rack this rack (see figure 2) has been designed specifically to aid in achieving precise and accurate results when using the immunoassay technique to analyze several samples at the same time. Figure 2 the 1-cm mi...

  • Page 100: Atrazine

    Atrazine atrazine page 6 of 8 atz_none_other_imm_eng_ody.Fm interpretation sample #1 — sample reading is less than the readings for both calibrators. Therefore the sample concentration of atrazine is greater than both 0.5 ppb and 3.0 ppb atrazine. Sample #2 — sample reading is between the readings f...

  • Page 101: Atrazine

    Atrazine atrazine atz_none_other_imm_eng_ody.Fm page 7 of 8 sample collection and storage collect samples in a clean glass bottle. Do not pre-rinse the bottle with the sample. If the sample cannot be analyzed immediately, store the sample at 4 °c. Samples may be kept for as long as 14 days. Warm the...

  • Page 102

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 103: Dr/2400

    Dr/2400 barium barium_avpp_other_tur_eng_ody.Fm page 1 of 6 • perform a standard curve adjustment or a new calibration for each new lot of reagent. See standard solutions and calibration standard preparation. • for more accurate results, determine a reagent blank value for each new lot of reagent. F...

  • Page 104: Barium

    Barium barium page 2 of 6 barium_avpp_other_tur_eng_ody.Fm 5. Fill another sample cell with 25 ml of sample (this is the blank). 6. When the timer beeps, wipe the blank and place it into the cell holder. 7. Touch zero . The display will show: 0 mg/l ba 2+ 8. Within 10 minutes after the timer beep, w...

  • Page 105: Barium

    Barium barium barium_avpp_other_tur_eng_ody.Fm page 3 of 6 interferences sample collection, storage, and preservation collect samples in an acid cleaned glass or plastic container. Adjust the ph to 2 or less with nitric acid (about 2 ml per liter) (cat. No. 2540-49). Preserved samples can be stored ...

  • Page 106: Barium

    Barium barium page 4 of 6 barium_avpp_other_tur_eng_ody.Fm standard solutions prepare a 90.0-mg/l barium standard solution as follows: 1. Pipet 9.00 ml of barium standard solution, 1000-mg/l, into a 100-ml volumetric flask. 2. Dilute to the mark with deionized water. 3. Prepare this solution daily. ...

  • Page 107: Barium

    Barium barium barium_avpp_other_tur_eng_ody.Fm page 5 of 6 method performance standard: 30 mg/l ba see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4.5 sensitivity on page 44 for more info...

  • Page 108

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 109: Dr/2400

    Dr/2400 benzotriazole or tolyltriazole benzotriazole_pp_other_uvp_eng_ody.Fm page 1 of 4 • sample temperature should be 20–25 °c (68–78 °f). • if sample contains nitrite or borax (sodium borate), adjust the ph to 4–6 with 1 n sulfuric acid (cat. No. 1270-32). • if the sample contains more than 500 m...

  • Page 110: Zero

    Benzotriazole or tolyltriazole benzotriazole or tolyltriazole page 2 of 4 benzotriazole_pp_other_uvp_eng_ody.Fm 5. Put on uv safety goggles. 6. Insert the ultraviolet lamp into the sample cell. Turn on the uv lamp. 7. Touch the timer icon. Touch ok . A 5-minute reaction period will begin. Low result...

  • Page 111

    Benzotriazole or tolyltriazole benzotriazole or tolyltriazole benzotriazole_pp_other_uvp_eng_ody.Fm page 3 of 4 sample collection, storage, and preservation the most reliable results are obtained when samples are analyzed as soon as possible after collection. Accuracy check standard additions method...

  • Page 112

    Benzotriazole or tolyltriazole © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 benzotriazole_pp_other_uvp_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-...

  • Page 113: Dr/2400

    Dr/2400 boron boron_pp_other_car_eng_ody.Fm page 1 of 4 • all labware must be completely dry. Excess water will cause low results. • use the borover ® 3 reagent with adequate ventilation. See reagent preparation, below. • do not cap the sample cells or the erlenmeyer flasks at any time during sample...

  • Page 114: Boron

    Boron boron page 2 of 4 boron_pp_other_car_eng_ody.Fm sample collection, preservation, and storage collect samples in clean polyethylene or polypropylene bottles, or alkali-resistant, boron-free glass. Reagent preparation to prepare additional borover 3/sulfuric acid solution, mix one borover 3 reag...

  • Page 115: Boron

    Boron boron boron_pp_other_car_eng_ody.Fm page 3 of 4 the borover 3/sulfuric acid solution is highly acidic. Neutralize to ph 6–9 and flush down the drain for disposal. For more information on waste management, see section 4. Accuracy check standard additions method (sample spike) 1. After reading t...

  • Page 116: Boron

    Boron © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 boron_pp_other_car_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office ...

  • Page 117: Dr/2400

    Dr/2400 boron boron_pp_low_azo_eng_ody.Fm page 1 of 6 • sample temperature should be 22–24 °c (72–75 °f) for most accurate results. If outside this range, measure and record the sample temperature. See sample temperature compensation on page 4. Boron method 10061 azomethine-h method* * adapted from ...

  • Page 118: Boron

    Boron boron page 2 of 6 boron_pp_low_azo_eng_ody.Fm 5. Open one pillow borotrace ™ 2 reagent and add the contents to the prepared sample. 6. Cap the prepared sample and shake to dissolve the powder. Proceed immediately with steps 7 and 8. 7. Touch the timer icon. Touch ok . A ten-minute reaction per...

  • Page 119: Boron

    Boron boron boron_pp_low_azo_eng_ody.Fm page 3 of 6 cell matching procedure 1. Rinse and fill two cells with deionized water. 2. Wipe each cell with a soft cloth or tissue. 3. Using one of the cells, set the instrument absorbance at 410 nm to zero. 4. Read the absorbance of the other cell. 5. Cells ...

  • Page 120: Boron

    Boron boron page 4 of 6 boron_pp_low_azo_eng_ody.Fm table 2 interfering substances and suggested treatments sample collection, preservation and storage collect samples in clean polyethylene bottles. Do not use borate-based detergents or soaps to clean sample containers or labware used for this metho...

  • Page 121: Boron

    Boron boron boron_pp_low_azo_eng_ody.Fm page 5 of 6 table 3 sample temperature multipliers accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. Verify the chemical form. 2. Touch options . Touch standard add...

  • Page 122: Boron

    Boron © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 boron_pp_low_azo_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or...

  • Page 123: Dr/2400

    Dr/2400 bromine bromine_avpp_other_dpd_eng_ody.Fm page 1 of 4 • analyze samples immediately. Do not preserve for later analysis. • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the r...

  • Page 124: Bromine

    Bromine bromine page 2 of 4 bromine_avpp_other_dpd_eng_ody.Fm 5. Fill a second sample cell with 10 ml of sample. (this is the blank.) 6. Wipe the blank and place it into the cell holder. 7. Touch zero . The display will show: 0.00 mg/l br 2 8. Within three minutes after the timer beeps, wipe the pre...

  • Page 125: Bromine

    Bromine bromine bromine_avpp_other_dpd_eng_ody.Fm page 3 of 4 interferences 5. Touch the timer icon. Touch ok . A three-minute reaction period will begin. Perform steps 6–7 during the reaction period. 6. Wipe the blank and place it into the cell holder. 7. Touch zero . The display will show: 0.00 mg...

  • Page 126: Bromine

    Bromine © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 bromine_avpp_other_dpd_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach o...

  • Page 127: Dr/2400

    Dr/2400 cadmium cadmium_unicell_other_cad_eng_ody.Fm page 1 of 4 • adjust the ph to between 3–6 with 5.0 n sodium hydroxide standard solution before analysis. • clean the outside of the vial before placing it in the instrument. Wipe with a wet towel, followed by a dry one. • use the standard solutio...

  • Page 128: Cadmium

    Cadmium cadmium page 2 of 4 cadmium_unicell_other_cad_eng_ody.Fm interferences the ions listed in the table have been tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. 5. Pipet 0.5 ml of stabilizer b (hct 154 b) into a sample vial. 6. ...

  • Page 129: Cadmium

    Cadmium cadmium cadmium_unicell_other_cad_eng_ody.Fm page 3 of 4 total cadmium, including undissolved cadmium and complexed cadmium can only be determined after digesting with the metal prep set, hct 200. Note: the total cadmium measuring range is 0.02–0.36 mg/l. Sample collection, storage, and pres...

  • Page 130: Cadmium

    Cadmium © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 cadmium_unicell_other_cad_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hac...

  • Page 131: Dr/2400

    Dr/2400 chloramine (mono) chloramine (mono)_avpp_high_mcf_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or per...

  • Page 132: Chloramine (Mono)

    Chloramine (mono) chloramine (mono) page 2 of 6 chloramine (mono)_avpp_high_mcf_eng_ody.Fm interferences the following have been tested for interference and found not to interfere up to the indicated levels: 5. Remove the vial from the adapter. Using a micro-funnel, add the contents of one monochlor...

  • Page 133: Chloramine (Mono)

    Chloramine (mono) chloramine (mono) chloramine (mono)_avpp_high_mcf_eng_ody.Fm page 3 of 6 sampling and storage analyze samples for monochloramine immediately after collection. Rinse the sample container several times with sample, letting the container overflow each time. If sampling from a tap, let...

  • Page 134: Chloramine (Mono)

    Chloramine (mono) chloramine (mono) page 4 of 6 chloramine (mono)_avpp_high_mcf_eng_ody.Fm 7. Open an ampule and use a glass mohr pipet to add the calculated amount of chlorine solution slowly to the ammonia standard, while mixing at medium speed on a stir-plate. 8. Allow the monochloramine solution...

  • Page 135: Chloramine (Mono)

    Chloramine (mono) chloramine (mono) chloramine (mono)_avpp_high_mcf_eng_ody.Fm page 5 of 6 safety good safety habits and laboratory techniques should be used throughout the procedure. Consult the material safety data sheet (msds) for information specific to the reagent used. Required reagents quanti...

  • Page 136

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 137: Dr/2400

    Dr/2400 chloramine (mono) chloramine (mono)_mp_low_mcf_eng_2400.Fm page 1 of 6 . • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or per...

  • Page 138: Chloramine (Mono)

    Chloramine (mono) chloramine (mono) page 2 of 6 chloramine (mono)_mp_low_mcf_eng_2400.Fm interferences the following have been tested for interference and found not to interfere up to the indicated levels: 5. Remove the cell from theadapter and add the contents of one pillow monochlor-f to the sampl...

  • Page 139: Chloramine (Mono)

    Chloramine (mono) chloramine (mono) chloramine (mono)_mp_low_mcf_eng_2400.Fm page 3 of 6 table 2 table 3 sampling and storage analyze samples for monochloramine immediately after collection. Rinse the sample container several times with sample, letting the container overflow each time. If sampling f...

  • Page 140: Chloramine (Mono)

    Chloramine (mono) chloramine (mono) page 4 of 6 chloramine (mono)_mp_low_mcf_eng_2400.Fm accuracy check to check test accuracy, prepare the following 4.5-mg/l (as cl 2 ) monochloramine standard fresh before use. 1. Add the contents of one buffer powder pillow, ph 8.3, to about 50-ml of organic-free ...

  • Page 141: Chloramine (Mono)

    Chloramine (mono) chloramine (mono) chloramine (mono)_mp_low_mcf_eng_2400.Fm page 5 of 6 sensitivity see section 3.4.5 sensitivity on page 43 for more information. Summary of method in the presence of a cyanoferrate catalyst, monochloramine (nh 2 cl) in the sample reacts with a substituted phenol to...

  • Page 142

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 143: Dr/2400

    Dr/2400 chloride chloride_none_other_mrt_eng_ody.Fm page 1 of 4 • filter turbid samples with moderately rapid filter paper (cat. No. 692-57) and a funnel (cat. No. 1083-68) before analysis. • both the sample and the blank will contain mercury (d009) at a concentration regulated as a hazardous waste ...

  • Page 144: Chloride

    Chloride chloride page 2 of 4 chloride_none_other_mrt_eng_ody.Fm interferences 5. Swirl to mix. 6. Pipet 1.0 ml of ferric ion solution into each sample cell. 7. Swirl to mix. An orange color will develop if chloride is present. 8. Touch the timer icon. Touch ok . A two-minute reaction time will begi...

  • Page 145: Chloride

    Chloride chloride chloride_none_other_mrt_eng_ody.Fm page 3 of 4 sample collection, storage, and preservation collect samples in glass or plastic containers. Samples can be stored for at least 28 days at room temperature. Accuracy check standard additions method (sample spike) 1. After reading test ...

  • Page 146: Chloride

    Chloride © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 chloride_none_other_mrt_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach...

  • Page 147: Dr/2400

    Dr/2400 chlorine dioxide chlorinedio_none_high_drm_eng_ody.Fm page 1 of 4 f • analyze samples immediately because chlorine dioxide is unstable and volatile. See interferences. • wipe the outside of sample cells before each insertion into the instrument cell holder. Use a damp towel followed by a dry...

  • Page 148: Chlorine Dioxide

    Chlorine dioxide chlorine dioxide page 2 of 4 chlorinedio_none_high_drm_eng_ody.Fm interferences sample collection, storage, and preservation analyze samples for chlorine dioxide immediately after collection. Chlorine dioxide is a strong oxidizing agent and is unstable in natural waters. It reacts r...

  • Page 149: Chlorine Dioxide

    Chlorine dioxide chlorine dioxide chlorinedio_none_high_drm_eng_ody.Fm page 3 of 4 demand by soaking in a dilute bleach solution (1 ml commercial bleach to l liter of deionized water) for at least one hour. Rinse thoroughly with deionized or distilled water. If sample containers are rinsed thoroughl...

  • Page 150

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 151: Dr/2400

    Dr/2400 chlorine dioxide chlorinedio_avpp_other_dpd_eng_ody.Fm page 1 of 6 • analyze samples immediately because chlorine dioxide is unstable and volatile. See sample collection, storage, and preservation. • for more accurate results, determine a reagent blank value for each new lot of reagent. Foll...

  • Page 152: Chlorine Dioxide

    Chlorine dioxide chlorine dioxide page 2 of 6 chlorinedio_avpp_other_dpd_eng_ody.Fm 5. Add four drops of glycine reagent to the sample. Swirl to mix. 6. Add the contents of one dpd free chlorine powder pillow to the prepared sample cell. Swirl the sample for 20 seconds to mix. 7. Wait 30 seconds for...

  • Page 153: Chlorine Dioxide

    Chlorine dioxide chlorine dioxide chlorinedio_avpp_other_dpd_eng_ody.Fm page 3 of 6 interferences 5. Add 16 drops of glycine reagent to the sample in the beaker. Swirl gently to mix. 6. Fill a dpd free chlorine reagent accuvac ampul with sample. Keep the tip immersed while the ampule fills completel...

  • Page 154: Chlorine Dioxide

    Chlorine dioxide chlorine dioxide page 4 of 6 chlorinedio_avpp_other_dpd_eng_ody.Fm sample collection, storage, and preservation analyze samples for chlorine dioxide immediately after collection. Chlorine dioxide is a strong oxidizing agent and is unstable in natural waters. It reacts rapidly with v...

  • Page 155: Chlorine Dioxide

    Chlorine dioxide chlorine dioxide chlorinedio_avpp_other_dpd_eng_ody.Fm page 5 of 6 c. Pipet the volume of standard needed into a 100-ml volumetric flask. Dilute to the line with chlorine-demand-free deionized water. Invert to mix. Method 2 a. Dilute 1 drop of 5% chlorine bleach in 1 liter of chlori...

  • Page 156: Chlorine Dioxide

    Chlorine dioxide © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 chlorinedio_avpp_other_dpd_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 conta...

  • Page 157: Dr/2400

    Dr/2400 chlorine dioxide chlorinedio_other_low_ama_eng_ody.Fm page 1 of 4 • analyze samples immediately because chlorine dioxide is unstable and volatile. See sample collection, storage and preservation. • for most accurate results, analyze each portion of sample at the same temperature. • wipe the ...

  • Page 158: Chlorine Dioxide

    Chlorine dioxide chlorine dioxide page 2 of 4 chlorinedio_other_low_ama_eng_ody.Fm interferences 5. Add 1.0 ml of chlorine dioxide reagent a into a second 25-ml volumetric flask. Note: use a volumetric pipet and pipet filler or a tensette pipet to add this reagent. 6. Fill the second volumetric flas...

  • Page 159: Chlorine Dioxide

    Chlorine dioxide chlorine dioxide chlorinedio_other_low_ama_eng_ody.Fm page 3 of 4 sample collection, storage and preservation analyze samples for chlorine dioxide immediately after collection. Chlorine dioxide is a strong oxidizing agent and is unstable in natural waters. It reacts rapidly with var...

  • Page 160: Chlorine Dioxide

    Chlorine dioxide © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 chlorinedio_other_low_ama_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contac...

  • Page 161: Dr/2400

    Dr/2400 chlorine dioxide chlorinedio_pp_low_cpr_eng_ody.Fm page 1 of 4 • analyze samples immediately because chlorine dioxide is unstable and volatile. See sample collection, storage and preservation. • for most accurate results, analyze each portion of sample at the same temperature. • wipe the out...

  • Page 162: Chlorine Dioxide

    Chlorine dioxide chlorine dioxide page 2 of 4 chlorinedio_pp_low_cpr_eng_ody.Fm interferences 5. Use a class a pipet to add exactly 1.00 ml of chlorine dioxide reagent 2 to each cylinder. Stopper. 6. Invert several times to mix. 7. Use a volumetric pipet and pipet filler to add 1.0 ml of chlorine di...

  • Page 163: Chlorine Dioxide

    Chlorine dioxide chlorine dioxide chlorinedio_pp_low_cpr_eng_ody.Fm page 3 of 4 sample collection, storage and preservation analyze samples for chlorine dioxide immediately after collection. Chlorine dioxide is a strong oxidizing agent and is unstable in natural waters. It reacts rapidly with variou...

  • Page 164: Chlorine Dioxide

    Chlorine dioxide © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 chlorinedio_pp_low_cpr_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact t...

  • Page 165: Dr/2400

    Dr/2400 chlorine, free chlorinefre_avpp_other_dpd_eng_ody.Fm page 1 of 6 • analyze samples immediately. Do not preserve for later analysis. • after adding reagent to the sample cell, a pink color will develop if free chlorine is present. • if the test overranges, dilute the sample with a known volum...

  • Page 166: Chlorine, Free

    Chlorine, free chlorine, free page 2 of 6 chlorinefre_avpp_other_dpd_eng_ody.Fm 5. Fill a second round cell with 10 ml of sample. 6. Add the contents of one dpd free chlorine powder pillow to the sample cell. (this is the prepared sample). Swirl the sample cell for 20 seconds to mix. Proceed to step...

  • Page 167: Chlorine, Free

    Chlorine, free chlorine, free chlorinefre_avpp_other_dpd_eng_ody.Fm page 3 of 6 interferences 5. Fill a dpd free chlorine reagent accuvac ampul with sample. Keep the tip immersed while the ampule fills completely. 6. Quickly invert the ampule several times to mix. Wipe off any liquid or fingerprints...

  • Page 168: Chlorine, Free

    Chlorine, free chlorine, free page 4 of 6 chlorinefre_avpp_other_dpd_eng_ody.Fm sample collection, storage and preservation analyze samples for chlorine immediately after collection. Free chlorine is a strong oxidizing agent and it is unstable in natural waters. It reacts rapidly with various inorga...

  • Page 169: Chlorine, Free

    Chlorine, free chlorine, free chlorinefre_avpp_other_dpd_eng_ody.Fm page 5 of 6 7. After completing the sequence, touch graph to view the best-fit line through the standard additions data points, accounting for matrix interferences. Touch view: fit , then select ideal line and touch ok to view the r...

  • Page 170

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 171: Dr/2400

    Dr/2400 chlorine, free chlorinefre_mp_high_dpd_eng_2400.Fm page 1 of 4 • analyze samples immediately. Do not preserve for later analysis. • after adding the dpd reagent, a pink color will develop if chlorine is present. • if the chlorine concentration is less than 2 mg/l, use method 8021, program nu...

  • Page 172: Chlorine, Free

    Chlorine, free chlorine, free page 2 of 4 chlorinefre_mp_high_dpd_eng_2400.Fm interferences 5. Remove the cell from the adapter and add the contents of one dpd free chlorine powder pillow to the sample. Cap and shake the cell about 20 seconds to dissolve. 6. Place the prepared sample into the adapte...

  • Page 173: Chlorine, Free

    Chlorine, free chlorine, free chlorinefre_mp_high_dpd_eng_2400.Fm page 3 of 4 sampling and storage analyze samples for chlorine immediately after collection. Free chlorine is a strong oxidizing agent and reacts rapidly with various compounds. Many factors such as sunlight, ph, temperature and sample...

  • Page 174: Chlorine, Free

    Chlorine, free © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 chlorinefre_mp_high_dpd_eng_2400.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact t...

  • Page 175: Dr/2400

    Dr/2400 chlorine, free chlorinefre_pt_other_dpd_eng_ody.Fm page 1 of 6 • analyze samples immediately. Do not preserve for later analysis. • clean the pour-thru cell and all labware as specified in treating analysis labware on page 4. • see reagent preparation on page 3 for instructions on preparing ...

  • Page 176: Chlorine, Free

    Chlorine, free chlorine, free page 2 of 6 chlorinefre_pt_other_dpd_eng_ody.Fm 5. Add 1.0 ml of free chlorine buffer solution to a clean, dry 100-ml glass mixing cylinder using the repipet jr. Dispenser. 6. Add 1.0 ml of prepared free chlorine indicator solution to the same mixing cylinder using the ...

  • Page 177: Chlorine, Free

    Chlorine, free chlorine, free chlorinefre_pt_other_dpd_eng_ody.Fm page 3 of 6 reagent preparation the free chlorine indicator solution must be prepared before use. Using a powder funnel, add the contents of one 24 g bottle of dpd powder (cat. No. 22972-55) to one 473-ml bottle of free chlorine indic...

  • Page 178: Chlorine, Free

    Chlorine, free chlorine, free page 4 of 6 chlorinefre_pt_other_dpd_eng_ody.Fm if sample containers are rinsed thoroughly with deionized water after use, only occasional pretreatment is necessary. A pre-treated bod bottle with a ground- glass stopper makes an ideal sample container for chlorine analy...

  • Page 179: Chlorine, Free

    Chlorine, free chlorine, free chlorinefre_pt_other_dpd_eng_ody.Fm page 5 of 6 method performance precision standard: 1.07 mg/l cl 2 see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4.5 sen...

  • Page 180

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 181: Dr/2400

    Dr/2400 chlorine, free chlorinefre_tnt_other_dpd_eng_ody.Fm page 1 of 4 • analyze samples immediately. Do not preserve samples for later analysis. • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the samp...

  • Page 182: Chlorine, Free

    Chlorine, free chlorine, free page 2 of 4 chlorinefre_tnt_other_dpd_eng_ody.Fm interferences 5. Remove the cap from a free chlorine dpd test ‘n tube. Add 10 ml of sample to the tube. (fill the vial to the top of the hach label. 6. Cap and invert at least 10 times to dissolve the powder. (this is the...

  • Page 183: Chlorine, Free

    Chlorine, free chlorine, free chlorinefre_tnt_other_dpd_eng_ody.Fm page 3 of 4 sample collection, storage and preservation analyze samples for chlorine immediately after collection. Free chlorine is a strong oxidizing agent and it is unstable in natural waters. It reacts rapidly with various inorgan...

  • Page 184: Chlorine, Free

    Chlorine, free © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 chlorinefre_tnt_other_dpd_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact ...

  • Page 185: Dr/2400

    Dr/2400 chlorine, total chlorinetot_avpp_other_dpd_eng_ody.Fm page 1 of 6 • samples must be analyzed immediately and cannot be preserved for later analysis. • after adding the reagent, a pink color will develop if chlorine is present. • if the test overranges, dilute the sample with a known volume o...

  • Page 186: Chlorine, Total

    Chlorine, total chlorine, total page 2 of 6 chlorinetot_avpp_other_dpd_eng_ody.Fm 5. Fill another round sample cell with 10-ml of sample. (this is the blank.) wipe the sample cell and place it into the cell holder. 6. Touch zero . The display will show: 0.00 mg/l cl 2 7. Within three minutes after t...

  • Page 187: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_avpp_other_dpd_eng_ody.Fm page 3 of 6 interferences 5. Touch the timer icon. Touch ok . A three-minute reaction period will begin. Performsteps 6 and 7 during this time period. 6. Wipe the blank and place it into the cell holder. 7. Touch zero . The displa...

  • Page 188: Chlorine, Total

    Chlorine, total chlorine, total page 4 of 6 chlorinetot_avpp_other_dpd_eng_ody.Fm sample collection, storage and preservation analyze samples for chlorine immediately after collection. Free chlorine is a strong oxidizing agent and it is unstable in natural waters. It reacts rapidly with various inor...

  • Page 189: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_avpp_other_dpd_eng_ody.Fm page 5 of 6 7. After completing the sequence, touch graph to view the best-fit line through the standard additions data points, accounting for matrix interferences. Touch view: fit , then select ideal line and touch ok to view the...

  • Page 190

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 191: Dr/2400

    Dr/2400 chlorine, total chlorinetot_mp_high_dpd_eng_2400.Fm page 1 of 4 • analyze samples immediately. Do not preserve for later analysis. • if chlorine is present, a pink color will develop after adding dpd total chlorine reagent. • if the chlorine concentration is typically less than 2 mg/l, use m...

  • Page 192: Chlorine, Total

    Chlorine, total chlorine, total page 2 of 4 chlorinetot_mp_high_dpd_eng_2400.Fm interferences 5. Remove the cell from the adapter and add the contents of one dpd total chlorine powder pillow to the sample. Cap and shake the cell about 20 seconds to dissolve. 6. Touch the timer icon. Touch ok . A 3-m...

  • Page 193: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_mp_high_dpd_eng_2400.Fm page 3 of 4 sampling and storage analyze samples for chlorine immediately after collection. Free and combined chlorine are strong oxidizing agents and react rapidly with various compounds. Many factors such as sunlight, ph, temperat...

  • Page 194: Chlorine, Total

    Chlorine, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 chlorinetot_mp_high_dpd_eng_2400.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact ...

  • Page 195: Dr/2400

    Dr/2400 chlorine, total chlorinetot_pt_other_dpd_eng_ody.Fm page 1 of 6 • analyze samples immediately. Do not preserve for later analysis. • clean the pour-thru cell and all labware as specified in treating analysis labware on page 4. • seereagent preparation on page 3 for instructions on preparing ...

  • Page 196: Chlorine, Total

    Chlorine, total chlorine, total page 2 of 6 chlorinetot_pt_other_dpd_eng_ody.Fm 5. Add 1.0 ml of total chlorine buffer solution to a clean, dry 100-ml glass mixing cylinder using the repipet jr. Dispenser. 6. Add 1.0 ml of prepared total chlorine indicator solution to the same mixing cylinder using ...

  • Page 197: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_pt_other_dpd_eng_ody.Fm page 3 of 6 reagent preparation the total chlorine indicator solution must be prepared before use. Using a powder funnel, add the contents of one 24 g bottle of dpd powder (cat. No. 22972-55) to one 473-ml bottle of total chlorine i...

  • Page 198: Chlorine, Total

    Chlorine, total chlorine, total page 4 of 6 chlorinetot_pt_other_dpd_eng_ody.Fm treating analysis labware glassware used in this test must be chlorine demand-free. Fill the 100 ml mixing cylinder and sample container with a dilute solution of chlorine bleach prepared by adding 1 ml of commercial ble...

  • Page 199: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_pt_other_dpd_eng_ody.Fm page 5 of 6 method performance precision standard: 1.07 mg/l cl 2 see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4.5 s...

  • Page 200

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 201: Dr/2400

    Dr/2400 chlorine, total chlorinetot_pt_ulow_dpd_eng_ody.Fm page 1 of 8 • analyze samples immediately. Chlorine is not stable in an aqueous solution. • a reagent blank value for a combined lot of indicator/buffer reagent solutions should be determined at least once a day. If sample color or turbidity...

  • Page 202: Chlorine, Total

    Chlorine, total chlorine, total page 2 of 8 chlorinetot_pt_ulow_dpd_eng_ody.Fm 5. When the timer beeps, touch zero . The display will show: 0 µg/l 6. Break open one ulr chlorine buffer solution ampule. 7. Using a tensette ® pipet and a clean tip, transfer 1.0 ml of buffer from the ampule to a clean,...

  • Page 203: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_pt_ulow_dpd_eng_ody.Fm page 3 of 8 13. Flush the pour-thru cell with at least 50 ml of deionized water immediately after use. 1. Touch hach programs . Select program 86 chlor. Total ulr . Touch start . 2. Make sure that the reagent blank setting is off. To...

  • Page 204: Chlorine, Total

    Chlorine, total chlorine, total page 4 of 8 chlorinetot_pt_ulow_dpd_eng_ody.Fm 5. Using a tensette ® pipet, add 1.0 ml of blanking reagent to the beaker. Swirl several times to mix. The blanking reagent removes chlorine and chloramines from the water. 6. Touch the timer icon, then the general timer ...

  • Page 205: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_pt_ulow_dpd_eng_ody.Fm page 5 of 8 interferences when the timer beeps, introduce the contents of the cylinder into the pour- thru cell. When the flow stops, touch read . The result in µg/l chlorine will appear. 13. Use this value to correct the sample resu...

  • Page 206: Chlorine, Total

    Chlorine, total chlorine, total page 6 of 8 chlorinetot_pt_ulow_dpd_eng_ody.Fm sample collection, storage, and preservation analyze samples for chlorine immediately after collection. Many factors, including reactant concentrations, sunlight, ph, temperature and salinity influence decomposition of ch...

  • Page 207: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_pt_ulow_dpd_eng_ody.Fm page 7 of 8 5. Prepare three sample spikes. Fill three mixing cylinders (cat. No. 1896-41) with 50 ml of sample. Use the tensette ® pipet to add 0.1 ml, 0.2 ml, and 0.3 ml of standard, respectively, to each sample and mix thoroughly....

  • Page 208: Chlorine, Total

    Chlorine, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 8 of 8 chlorinetot_pt_ulow_dpd_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact t...

  • Page 209: Dr/2400

    Dr/2400 chlorine, total chlorinetot_pt2_ulow_dpd_eng_ody.Fm page 1 of 10 • analyze samples immediately. Chlorine is not stable in an aqueous solution. • a reagent blank value for a combined lot of indicator/buffer reagent solutions should be determined at least once a day. See determining the reagen...

  • Page 210: Chlorine, Total

    Chlorine, total chlorine, total page 2 of 10 chlorinetot_pt2_ulow_dpd_eng_ody.Fm 5. Break open one ulr chlorine buffer solution ampule. 6. Using a tensette ® pipet and a clean tip, transfer 1.0 ml of buffer from the ampule to a clean, treated 50-ml graduated mixing cylinder. 7. Break open one ampule...

  • Page 211: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_pt2_ulow_dpd_eng_ody.Fm page 3 of 10 13. Pour the filtered sample from the plunger reservoir into the pour-thru cell. 14. After the flow stops, touch zero . The display will show: 0 µg/l cl 2 15. Pull the barrel’s valve button out to the “open” position. P...

  • Page 212: Chlorine, Total

    Chlorine, total chlorine, total page 4 of 10 chlorinetot_pt2_ulow_dpd_eng_ody.Fm 21. Determine a reagent blank using the procedure following these steps. 1. Touch hach programs . Select program 86 chlor. Total ulr . Touch start . 2. Make sure that the reagent blank setting is off. Touch options , th...

  • Page 213: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_pt2_ulow_dpd_eng_ody.Fm page 5 of 10 5. Using a tensette ® pipet, add 1.0 ml of blanking reagent to the beaker. Swirl several times to mix. The blanking reagent removes chlorine and chloramines from the water. 6. Touch the timer icon, then the general time...

  • Page 214: Chlorine, Total

    Chlorine, total chlorine, total page 6 of 10 chlorinetot_pt2_ulow_dpd_eng_ody.Fm interferences 13. When the timer beeps, introduce the contents of the cylinder into the pour-thru cell. When the flow stops, touch read . Results will appear in µg/l cl 2 . 14. Use this value to correct the sample resul...

  • Page 215: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_pt2_ulow_dpd_eng_ody.Fm page 7 of 10 sample collection, storage and preservation analyze samples for chlorine immediately after collection. Free chlorine is a strong oxidizing agent and it is unstable in natural waters. It reacts rapidly with various inorg...

  • Page 216: Chlorine, Total

    Chlorine, total chlorine, total page 8 of 10 chlorinetot_pt2_ulow_dpd_eng_ody.Fm 5. Prepare three sample spikes. Fill three mixing cylinders (cat. No. 1896-41) with 50 ml of sample. Use the tensette ® pipet to add 0.1 ml, 0.2 ml, and 0.3 ml of standard, respectively, to each sample and mix thoroughl...

  • Page 217: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_pt2_ulow_dpd_eng_ody.Fm page 9 of 10 required reagents quantity required description per test unit cat. No ulr chlorine reagent set (about 20 tests) ..................................................................................................25630-00 ...

  • Page 218

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 219: Dr/2400

    Dr/2400 chlorine, total chlorinetot_tnt_other_dpd_eng_ody.Fm page 1 of 4 • analyze samples immediately. Do not preserve samples for later analysis. • after adding sample to the test ‘n tube™, a pink color will develop if total chlorine is present. • for more accurate results, determine a reagent bla...

  • Page 220: Chlorine, Total

    Chlorine, total chlorine, total page 2 of 4 chlorinetot_tnt_other_dpd_eng_ody.Fm interferences 5. Touch zero . The display will show: 0.00 mg/l cl 2 6. Remove the cap from a free chlorine dpd test ‘n tube™. Add 10 ml of sample to the tube. (fill the vial to the top of the hach label.) this is the pr...

  • Page 221: Chlorine, Total

    Chlorine, total chlorine, total chlorinetot_tnt_other_dpd_eng_ody.Fm page 3 of 4 sample collection, storage and preservation analyze samples for chlorine immediately after collection. Free chlorine and combined chlorine are strong oxidizing agents and are unstable in natural waters. Many factors, in...

  • Page 222: Chlorine, Total

    Chlorine, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 chlorinetot_tnt_other_dpd_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact...

  • Page 223: Dr/2400

    Dr/2400 chromium, hexavalent chromiumhex_avpp_other_dch_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. See the instrument manual for more information on running a reag...

  • Page 224: Chromium, Hexavalent

    Chromium, hexavalent chromium, hexavalent page 2 of 6 chromiumhex_avpp_other_dch_eng_ody.Fm 5. Fill another round sample cell with 10 ml of sample (the blank). 6. When the timer beeps, place the blank into the cell holder. 7. Touch zero . The display will show: 0.00 mg/l cr 6+ 8. Place the prepared ...

  • Page 225: Chromium, Hexavalent

    Chromium, hexavalent chromium, hexavalent chromiumhex_avpp_other_dch_eng_ody.Fm page 3 of 6 interferences sample collection, preservation, and storage collect samples in a cleaned glass or plastic container. Store at 4 °c (39 °f) up to 24 hours. Samples must be analyzed within 24 hours. Accuracy che...

  • Page 226: Chromium, Hexavalent

    Chromium, hexavalent chromium, hexavalent page 4 of 6 chromiumhex_avpp_other_dch_eng_ody.Fm 5. For analysis using powder pillows, use the tensette ® pipet to add 0.1 ml, 0.2 ml, and 0.3 ml of standard, respectively to three 25-ml samples and mix thoroughly. Transfer 10 ml of each solution into a 10-...

  • Page 227: Chromium, Hexavalent

    Chromium, hexavalent chromium, hexavalent chromiumhex_avpp_other_dch_eng_ody.Fm page 5 of 6 summary of method hexavalent chromium is determined by the 1,5-diphenylcarbohydrazide method using a single dry powder formulation called chromaver 3 chromium reagent. This reagent contains an acidic buffer c...

  • Page 228

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 229: Dr/2400

    Dr/2400 chromium, hexavalent chromiumhex_unicell_other_dch_eng_ody.Fm page 1 of 4 • undissolved chromium can only be determined after digestion with the metal prep set (hct 200). • adjust ph of preserved samples to between ph 3–9 before analysis. • clean the outside of the vial before placing it in ...

  • Page 230: Chromium, Hexavalent

    Chromium, hexavalent chromium, hexavalent page 2 of 4 chromiumhex_unicell_other_dch_eng_ody.Fm interferences the ions listed in the table have been tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. Higher amounts of iron, copper, and r...

  • Page 231: Chromium, Hexavalent

    Chromium, hexavalent chromium, hexavalent chromiumhex_unicell_other_dch_eng_ody.Fm page 3 of 4 sample collection, preservation, and storage collect samples in a cleaned glass or plastic container. Store at 4 °c (39 °f) up to 24 hours. Samples must be analyzed within 24 hours. Accuracy check standard...

  • Page 232: Chromium, Hexavalent

    Chromium, hexavalent © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 chromiumhex_unicell_other_dch_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-422...

  • Page 233: Dr/2400

    Dr/2400 chromium, total chromiumtot_pp_other_ahy_eng_ody.Fm page 1 of 4 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform a re...

  • Page 234: Chromium, Total

    Chromium, total chromium, total page 2 of 4 chromiumtot_pp_other_ahy_eng_ody.Fm 5. Touch the timer icon. Touch ok . A five-minute reaction period will begin. 6. When the timer beeps, remove the prepared sample. Using running water, cool the cell to 25 °c. Be sure the caps are on tightly. 7. Remove c...

  • Page 235: Chromium, Total

    Chromium, total chromium, total chromiumtot_pp_other_ahy_eng_ody.Fm page 3 of 4 interferences sample collection, storage and preservation collect samples in acid-washed glass or plastic containers. To preserve samples, adjust the ph to 2 or less with nitric acid (cat. No. 152-49). This requires appr...

  • Page 236: Chromium, Total

    Chromium, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 chromiumtot_pp_other_ahy_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact ...

  • Page 237: Dr/2400

    Dr/2400 chromium, total chromiumtot_unicell_other_dch_eng_ody.Fm page 1 of 4 • adjust ph of preserved samples to between ph 3–9 before analysis. • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel, followed by a dry one. • use the standard solution or standard...

  • Page 238: Chromium, Total

    Chromium, total chromium, total page 2 of 4 chromiumtot_unicell_other_dch_eng_ody.Fm interferences the ions listed in the table have been tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. 5. Heat the vial in the reactor block for 60 mi...

  • Page 239: Chromium, Total

    Chromium, total chromium, total chromiumtot_unicell_other_dch_eng_ody.Fm page 3 of 4 larger amounts of iron, copper, reducing or oxidizing agents give low results. Lead, mercury, and tin give high results. Note: undissolved chromium is not determined with the determination of chromium vi. Total chro...

  • Page 240: Chromium, Total

    Chromium, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 chromiumtot_unicell_other_dch_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 con...

  • Page 241: Dr/2400

    Dr/2400 cobalt cobalt_pp_other_pan_eng_ody.Fm page 1 of 4 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank ad...

  • Page 242: Cobalt

    Cobalt cobalt page 2 of 4 cobalt_pp_other_pan_eng_ody.Fm 5. Use the plastic dropper provided to add 0.5 ml of 0.3% pan indicator solution to each cell. Stopper each cell. Invert several times to mix. 6. Touch the timer icon. Touch ok . A three-minute reaction period will begin. During the reaction p...

  • Page 243: Cobalt

    Cobalt cobalt cobalt_pp_other_pan_eng_ody.Fm page 3 of 4 interferences sample collection, storage and preservation collect samples in acid-washed plastic bottles. Adjust the sample ph to 2 or less with nitric acid (cat. No. 2540-49) (about 5 ml per liter). Preserved samples can be stored up to six m...

  • Page 244: Cobalt

    Cobalt © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 cobalt_pp_other_pan_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach offic...

  • Page 245: Dr/2400

    Dr/2400 color, true and apparent colortru_none_other_pcs_eng_ody.Fm page 1 of 4 • ncasi procedure requires ph adjustment. Adjust the ph to 7.6 with 1.0 n hcl or 1.0 n naoh. When adjusting the ph, if overall volume change is greater than 1%, start over and use a stronger acid or base. • wipe the outs...

  • Page 246: Color, True and Apparent

    Color, true and apparent color, true and apparent page 2 of 4 colortru_none_other_pcs_eng_ody.Fm sample collection, storage and preservation collect samples in clean plastic or glass bottles. Most reliable results are obtained when samples are analyzed as soon as possible after collection. If prompt...

  • Page 247: Color, True and Apparent

    Color, true and apparent color, true and apparent colortru_none_other_pcs_eng_ody.Fm page 3 of 4 accuracy check standard solution method prepare a 250 platinum-cobalt units standard as follows: 1. Using class a glassware, pipet 50.00 ml of a 500 platinum-cobalt units standard solution into a 100-ml ...

  • Page 248: Color, True and Apparent

    Color, true and apparent © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 colortru_none_other_pcs_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 ...

  • Page 249: Dr/2400

    Dr/2400 copper copper_avpp_other_bic_eng_ody.Fm page 1 of 6 • digestion is required for determining total copper. See section 4 for the digestion procedure. • the chemistry is ph sensitive. Adjust the ph of acid-preserved samples to 4 –6 with 8 n koh before analysis. • for more accurate results, det...

  • Page 250: Copper

    Copper copper page 2 of 6 copper_avpp_other_bic_eng_ody.Fm 5. When the timer beeps, fill a second sample cell (the blank) with 10 ml of sample. Place the blank into the cell holder. 6. Touch zero . The display will show: 0.00 mg/l cu 7. Within 30 minutes after the timer beeps, place the prepared sam...

  • Page 251: Copper

    Copper copper copper_avpp_other_bic_eng_ody.Fm page 3 of 6 interferences to differentiate free copper from that complexed to edta or other complexing agents, use a free copper reagent powder pillow in place of the cuver 1 powder pillow in step 3. Results in step 8 will be free copper only. Add a hyd...

  • Page 252: Copper

    Copper copper page 4 of 6 copper_avpp_other_bic_eng_ody.Fm sample collection, storage and preservation collect samples in acid-cleaned glass or plastic containers. Adjust the ph to 2 or less with concentrated nitric acid (about 2 ml per liter) (cat. No. 2540-49). Store preserved samples up to six mo...

  • Page 253: Copper

    Copper copper copper_avpp_other_bic_eng_ody.Fm page 5 of 6 above. Accept each standard additions reading by touching read. Each addition should reflect approximately 100% recovery. 8. After completing the sequence, touch graph to view the best-fit line through the standard additions data points, acc...

  • Page 254: Copper

    Copper © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 copper_avpp_other_bic_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach off...

  • Page 255: Dr/2400

    Dr/2400 copper copper_pp_low_por_eng_ody.Fm page 1 of 6 • digestion is required for determining total copper. See section 4 for digestion procedure. • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sa...

  • Page 256: Copper

    Copper copper page 2 of 6 copper_pp_low_por_eng_ody.Fm 5. Add the contents of one porphyrin 2 reagent powder pillow to each sample cell. Swirl to dissolve. Note: if copper is present, the sample will momentarily turn blue, then return to yellow. 6. Touch the timer icon. Touch ok . A 3-minute reactio...

  • Page 257: Copper

    Copper copper copper_pp_low_por_eng_ody.Fm page 3 of 6 interferences sample collection, storage and preservation collect samples in acid-washed plastic bottles. To preserve, adjust the ph to 2 or less with nitric acid (about 5 ml per liter). Store preserved samples up to six months at room temperatu...

  • Page 258: Copper

    Copper copper page 4 of 6 copper_pp_low_por_eng_ody.Fm 4. Touch ok to accept the default values for standard concentration, sample volume, and spike volumes. Touch edit to change these values. After values are accepted, the unspiked sample reading will appear in the top row. See standard additions i...

  • Page 259: Copper

    Copper copper copper_pp_low_por_eng_ody.Fm page 5 of 6 summary of method the porphyrin method is very sensitive to trace amounts of free copper. The method is free from most interferences and does not require any sample extraction or concentration before analysis. Interferences from other metals are...

  • Page 260

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 261: Dr/2400

    Dr/2400 copper copper_unicell_other_bath_eng_ody.Fm page 1 of 4 • undissolved copper and copper contained in complexes can only be determined after digestion with the metal prep set (hct 200). The measuring range for total copper is 0.12–7.20 mg/l. • adjust ph of preserved samples to between ph 3–9 ...

  • Page 262: Copper

    Copper copper page 2 of 4 copper_unicell_other_bath_eng_ody.Fm interferences the ions listed in the table have been tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. Note: higher amounts of iron and chromium cause a positive interferen...

  • Page 263: Copper

    Copper copper copper_unicell_other_bath_eng_ody.Fm page 3 of 4 accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Touch options . Touch standard additions . A summary of the standard additions procedure...

  • Page 264: Copper

    Copper © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 copper_unicell_other_bath_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach...

  • Page 265: Dr/2400

    Dr/2400 cyanide cyanide_pp_other_ppz_eng_ody.Fm page 1 of 8 • use a water bath to maintain the optimum temperature for the reaction in this test (25 °c). Samples at less than 23 °c require longer reaction times, and samples at greater than 25 °c yield low results. • for more accurate results, determ...

  • Page 266: Cyanide

    Cyanide cyanide page 2 of 8 cyanide_pp_other_ppz_eng_ody.Fm 5. Leave the sample cell undisturbed for an additional 30 seconds. 6. Add the contents of one cyaniver 4 cyanide reagent powder pillow. Cap the sample cell. 7. Shake the sample for 10 seconds. Immediately proceed to step 8. (delaying the ad...

  • Page 267: Cyanide

    Cyanide cyanide cyanide_pp_other_ppz_eng_ody.Fm page 3 of 8 pollution prevention and waste management special considerations for cyanide containing materials samples analyzed by this procedure may contain cyanide, which is regulated as reactive (d003) waste by the federal rcra. It is imperative thes...

  • Page 268: Cyanide

    Cyanide cyanide page 4 of 8 cyanide_pp_other_ppz_eng_ody.Fm sample collection, storage and preservation collect samples in glass or plastic bottles and analyze as quickly as possible. The presence of oxidizing agents, sulfides and fatty acids can cause the loss of cyanide during sample storage. Samp...

  • Page 269: Cyanide

    Cyanide cyanide cyanide_pp_other_ppz_eng_ody.Fm page 5 of 8 d. If step 3 suggests the presence of oxidizing agents, add two level, 1-g measuring spoonfuls of ascorbic acid (cat. No. 6138-26) per liter of sample. E. Withdraw a 25-ml portion of sample treated with ascorbic acid and repeat steps 1 to 3...

  • Page 270: Cyanide

    Cyanide cyanide page 6 of 8 cyanide_pp_other_ppz_eng_ody.Fm if thiocyanate is present in the original sample, a distillation step is absolutely necessary as thiocyanate causes a positive interference. High concentrations of thiocyanate can yield a substantial quantity of sulfide in the distillate. T...

  • Page 271: Cyanide

    Cyanide cyanide cyanide_pp_other_ppz_eng_ody.Fm page 7 of 8 g. Using a water bottle, rinse the thistle tube with a small amount of deionized water. H. Allow the solution to mix for three minutes; then add 20 ml magnesium chloride reagent (cat. No. 14762-53) through the thistle tube and rinse again. ...

  • Page 272: Cyanide

    Cyanide © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 8 of 8 cyanide_pp_other_ppz_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach off...

  • Page 273: Dr/2400

    Dr/2400 cyanide cyanide_unicell_other_ppz_eng_ody.Fm page 1 of 4 • analyze samples collected in glass or plastic bottles as quickly as possible. • adjust ph of preserved samples to between ph 7–10 with 1.0 n sulfuric acid standard solution before analysis. • clean the outside of the vial before plac...

  • Page 274: Cyanide

    Cyanide cyanide page 2 of 4 cyanide_unicell_other_ppz_eng_ody.Fm interferences interferences are caused by substances like formaldehyde which react with the cyanide. Reducing agents (like sulfite) and other compounds that affect the reactivity of chlorine also interfere. Thiocyanate and cyanide reac...

  • Page 275: Cyanide

    Cyanide cyanide cyanide_unicell_other_ppz_eng_ody.Fm page 3 of 4 accuracy check standard additions method (sample spike) caution: cyanides and their solutions, and the hydrogen cyanide liberated by acids, are very poisonous. Both the solutions and the gas can be absorbed through the skin. 1. Prepare...

  • Page 276: Cyanide

    Cyanide © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 cyanide_unicell_other_ppz_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hac...

  • Page 277: Dr/2400

    Dr/2400 cyanuric acid cyanuricacid_pp_other_tur_eng_ody.Fm page 1 of 4 • filter highly turbid samples with filter paper (cat. No. 1894-57) and a funnel (cat. No.1083-67). • after adding the reagent, a white turbidity will form if cyanuric acid is present. • clean sample cells with soap, water, and a...

  • Page 278: Cyanuric Acid

    Cyanuric acid cyanuric acid page 2 of 4 cyanuricacid_pp_other_tur_eng_ody.Fm sampling and storage collect samples in clean plastic or glass bottles. Samples must be analyzed within 24 hours. Accuracy check standard solution method dissolve 1.000 gram of cyanuric acid in l liter of deionized water to...

  • Page 279: Cyanuric Acid

    Cyanuric acid cyanuric acid cyanuricacid_pp_other_tur_eng_ody.Fm page 3 of 4 method performance precision standard: 10 mg/l cyanuric acid see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4...

  • Page 280

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 281: Dr/2400

    Dr/2400 fluoride fluoride_accuvac_other_spd_eng_ody.Fm page 1 of 6 • the sample and deionized water should be at the same temperature (±1 °c). Temperature adjustments may be made before or after reagent addition. • spadns reagent is toxic and corrosive. Use care while handling the reagent. • for bes...

  • Page 282: Fluoride

    Fluoride fluoride page 2 of 6 fluoride_accuvac_other_spd_eng_ody.Fm 5. Touch the timer icon. Touch ok . A one-minute reaction period will begin. 6. When the timer beeps, place the blank into the cell holder. 7. Touch zero . The display will show: 0.00 mg/l f – 8. Place the prepared sample into the c...

  • Page 283: Fluoride

    Fluoride fluoride fluoride_accuvac_other_spd_eng_ody.Fm page 3 of 6 interferences this test is sensitive to small amounts of interference. Glassware must be very clean (acid rinse before each use). Repeat the test with the same glassware to ensure that results are accurate. Distillation 1. Most inte...

  • Page 284: Fluoride

    Fluoride fluoride page 4 of 6 fluoride_accuvac_other_spd_eng_ody.Fm 3. Turn on the water and maintain a steady flow through the condenser. 4. Measure 100 ml of sample into the distillation flask using a 100-ml graduated cylinder. Add a magnetic stir bar and 5 glass beads. 5. For proof of accuracy, u...

  • Page 285: Fluoride

    Fluoride fluoride fluoride_accuvac_other_spd_eng_ody.Fm page 5 of 6 method performance precision standard: 1.00 mg/l f - see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4.5 sensitivity on...

  • Page 286: Fluoride

    Fluoride © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 fluoride_accuvac_other_spd_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the h...

  • Page 287: Dr/2400

    Dr/2400 fluoride fluoride_unicell_other_spa_eng_ody.Fm page 1 of 4 • analyze samples collected in acid-cleaned glass or plastic bottles as quickly as possible. • adjust the ph of the sample to between 1–11 with hydrochloric acid before analysis. • clean the outside of the vial before placing it in t...

  • Page 288: Fluoride

    Fluoride fluoride page 2 of 4 fluoride_unicell_other_spa_eng_ody.Fm interferences the ions listed in the table have been tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. Sample collection, preservation, and storage collect samples in ...

  • Page 289: Fluoride

    Fluoride fluoride fluoride_unicell_other_spa_eng_ody.Fm page 3 of 4 3. Touch ok to accept the default values for standard concentration, sample volume, and spike volumes. Touch edit to change these values. After values are accepted, the unspiked sample reading will appear in the top row. See standar...

  • Page 290: Fluoride

    Fluoride © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 fluoride_unicell_other_spa_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the h...

  • Page 291: Dr/2400

    Dr/2400 formaldehyde formaldehyde_pp_other_mbt_eng_ody.Fm page 1 of 6 • analyze samples immediately. Do not preserve for later analysis. • wash glassware with chromic acid cleaning solution (cat. No. 1233-49) to remove trace contaminants. • wipe the outside of sample cells before each insertion into...

  • Page 292: Formaldehyde

    Formaldehyde formaldehyde page 2 of 6 formaldehyde_pp_other_mbt_eng_ody.Fm 5. Immediately touch the timer icon. A 17-minute reaction period will begin. Proceed with step 6 immediately after the timer starts. Complete steps 6–11 during the reaction period, at the times specified. 6. Immediately after...

  • Page 293: Formaldehyde

    Formaldehyde formaldehyde formaldehyde_pp_other_mbt_eng_ody.Fm page 3 of 6 interferences accuracy check 13. When the timer shows 2:00 , touch zero . The display will show: 0 µg/l ch 2 o 14. Pour the prepared sample into a sample cell. Wipe the cell and place it into the cell holder. 15. Wipe the cel...

  • Page 294: Formaldehyde

    Formaldehyde formaldehyde page 4 of 6 formaldehyde_pp_other_mbt_eng_ody.Fm standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Touch options . Touch standard additions . A summary of the standard additions procedure wi...

  • Page 295: Formaldehyde

    Formaldehyde formaldehyde formaldehyde_pp_other_mbt_eng_ody.Fm page 5 of 6 formaldehyde reacts with mbth (3-methyl-2-benzothiazoline hydrazone) and a developing solution to form a blue color in proportion to the formaldehyde concentration. Test results are measured at 630 nm. Required reagents quant...

  • Page 296

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 297: Dr/2400

    Dr/2400 hardness hardness_none_other_clc_eng_ody.Fm page 1 of 4 • for the most accurate magnesium test results, keep the sample temperature between 21–29 °c (70–84 °f). • the test will detect any calcium or magnesium contamination in the mixing cylinder, measuring droppers, or sample cells. To test ...

  • Page 298: Hardness

    Hardness hardness page 2 of 4 hardness_none_other_clc_eng_ody.Fm 9. Add one drop of egta solution to the second cell. Swirl to mix. 10. Place first cell (the blank) into the cell holder. 11. Touch zero . The display will show: 0.00 ppm mg caco 3 12. Place the second cell into the cell holder. Touch ...

  • Page 299: Hardness

    Hardness hardness hardness_none_other_clc_eng_ody.Fm page 3 of 4 interferences sample collection, storage and preservation collect samples in acid-washed plastic bottles. Adjust the sample ph to 2 or less with nitric acid (about 5 ml per liter) (cat. No. 152-49). Cool samples to 4 °c. Preserved samp...

  • Page 300: Hardness

    Hardness © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 hardness_none_other_clc_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach...

  • Page 301: Dr/2400

    Dr/2400 hardness, total hardness_mp_ulow_cpz_eng_2400.Fm page 1 of 6 • pre-clean the pour-thru cell and all labware as specified in treating analysis labware on page 3. • protect the pour-thru cell from contamination when not in use by inverting a small beaker over the top of the glass funnel. • if ...

  • Page 302: Hardness, Total

    Hardness, total hardness, total page 2 of 6 hardness_mp_ulow_cpz_eng_2400.Fm 5. Add the contents of one chlorophosphonazo solution pillow to the sample cell. Note: a small amount of solution may remain in the pillow. This will not affect results. Note: one ml of chlorophosphonazo solution (cat. No. ...

  • Page 303: Hardness, Total

    Hardness, total hardness, total hardness_mp_ulow_cpz_eng_2400.Fm page 3 of 6 interferences interference studies were conducted at various hardness levels between 0 and 500 µg/l as caco 3 . Various cations and anions were evaluated at levels in the range appropriate to ultra pure water applications. ...

  • Page 304: Hardness, Total

    Hardness, total hardness, total page 4 of 6 hardness_mp_ulow_cpz_eng_2400.Fm accuracy check standard additions method (sample spike) 1. Touch options . Touch standard additions . A summary of the standard additions procedure will appear. 2. Touch edit. Change the sample volume to read 25 ml. Change ...

  • Page 305: Hardness, Total

    Hardness, total hardness, total hardness_mp_ulow_cpz_eng_2400.Fm page 5 of 6 summary of method calcium and magnesium combine equivalently with the chlorophosphonazo indicator to form a colored complex which absorbs light very strongly at 669 nm. One drop of the cdta reagent breaks up this complex, a...

  • Page 306

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 307: Dr/2400

    Dr/2400 hardness, total hardness_pt_ulow_cpz_eng_ody.Fm page 1 of 6 • pre-clean the pour-thru cell and all labware as specified in treating analysis labware on page 4. • protect the pour-thru cell from contamination when not in use by inverting a small beaker over the top of the glass funnel. • if t...

  • Page 308: Hardness, Total

    Hardness, total hardness, total page 2 of 6 hardness_pt_ulow_cpz_eng_ody.Fm 5. Fill this rinsed cylinder to the 50-ml mark with sample from the flask. Discard remaining contents of the flask. 6. Pour the contents of the 50-ml cylinder back into the flask. 7. Add 2.0 ml of chlorophosphonazo reagent t...

  • Page 309: Hardness, Total

    Hardness, total hardness, total hardness_pt_ulow_cpz_eng_ody.Fm page 3 of 6 interferences interference studies were conducted at various hardness levels between 0 and 500 µg/l as caco 3 . Various cations and anions were evaluated at levels in the range appropriate to ultra pure water applications. A...

  • Page 310: Hardness, Total

    Hardness, total hardness, total page 4 of 6 hardness_pt_ulow_cpz_eng_ody.Fm treating analysis labware clean all containers used in this test thoroughly to remove any traces of calcium or magnesium. If possible, use plastic containers for all analysis and storage. Clean containers by normal means, th...

  • Page 311: Hardness, Total

    Hardness, total hardness, total hardness_pt_ulow_cpz_eng_ody.Fm page 5 of 6 6. After completing the sequence, touch graph to view the best-fit line through the standard additions data points, accounting for matrix interferences. Touch view: fit , then select ideal line and touch ok to view the relat...

  • Page 312

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 313: Dr/2400

    Dr/2400 hydrazine hydrazine_accuvac_other_pda_eng_ody.Fm page 1 of 4 • samples cannot be preserved and must be analyzed immediately. • sample temperature should be 21 ±4 °c (70 ±7 °f). • after adding the hydraver ® 2 hydrazine reagent, a yellow color will develop in the sample if hydrazine is presen...

  • Page 314: Hydrazine

    Hydrazine hydrazine page 2 of 4 hydrazine_accuvac_other_pda_eng_ody.Fm 5. Touch the timer icon. Touch ok . A 12-minute reaction period will begin. Complete steps 6–8 during this period. 6. Place the blank into the cell holder. 7. Touch zero . The display will show: 0 µg/l n 2 h 4 8. Place the prepar...

  • Page 315: Hydrazine

    Hydrazine hydrazine hydrazine_accuvac_other_pda_eng_ody.Fm page 3 of 4 interferences sample collection, storage and preservation samples collected in glass or plastic bottles should be filled completely and capped tightly. Avoid excessive agitation or exposure to air. Samples must be analyzed immedi...

  • Page 316: Hydrazine

    Hydrazine © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 hydrazine_accuvac_other_pda_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the...

  • Page 317: Dr/2400

    Dr/2400 iodine iodine_avpp_other_dpd_eng_ody.Fm page 1 of 4 • analyze samples immediately. Do not preserve for later analysis. • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the rea...

  • Page 318: Iodine

    Iodine iodine page 2 of 4 iodine_avpp_other_dpd_eng_ody.Fm 5. Fill a second sample cell with 10 ml of sample (this is the blank). Wipe the blank and place it into the cell holder. Close the cover. 6. Touch zero . The display will show: 0.00 mg/l i 2 7. Within three minutes after the timer beeps, wip...

  • Page 319: Iodine

    Iodine iodine iodine_avpp_other_dpd_eng_ody.Fm page 3 of 4 interferences 5. Touch the timer icon. Touch ok . A three-minute reaction period will begin. Perform steps 6-7 during the reaction period. 6. Wipe the blank and place it into the cell holder. 7. Touch zero . The display will show: 0.00 mg/l ...

  • Page 320: Iodine

    Iodine © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 iodine_avpp_other_dpd_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach off...

  • Page 321: Dr/2400

    Dr/2400 iron iron_pp_other_fez_eng_ody.Fm page 1 of 4 • digestion is required for total iron determination. See section 4 on page 55 for the digestion procedure. • rinse glassware with a 1:1 hydrochloric acid solution. Rinse again with deionized water. These two steps will remove iron deposits that ...

  • Page 322: Iron

    Iron iron page 2 of 4 iron_pp_other_fez_eng_ody.Fm interferences 5. Fill another sample cell with 25 ml of sample (this is the blank). 6. When the timer beeps, insert the blank into the cell holder. 7. Touch zero . The display will show: 0.000 mg/l fe 8. Place the prepared sample into the cell holde...

  • Page 323: Iron

    Iron iron iron_pp_other_fez_eng_ody.Fm page 3 of 4 sample collection, storage and preservation collect samples in acid-washed glass or plastic bottles. To preserve samples, adjust the sample ph to 2 or less with concentrated nitric acid, acs (cat. No. 152-49) (about 2 ml per liter). Samples preserve...

  • Page 324: Iron

    Iron © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 iron_pp_other_fez_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or...

  • Page 325: Dr/2400

    Dr/2400 iron iron_unicell_other_phen_eng_ody.Fm page 1 of 4 • undissolved iron and complexed iron can only be determined after digestion using the metal prep set (hct 200). Total iron measuring range is 0.12–6.00 mg/l. • underrange appears on the display when the instrument is zeroed or when the det...

  • Page 326: Iron

    Iron iron page 2 of 4 iron_unicell_other_phen_eng_ody.Fm interferences the ions listed in the table have been tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. Higher amounts of copper, nickel, and tin cause a positive interference. Sa...

  • Page 327: Iron

    Iron iron iron_unicell_other_phen_eng_ody.Fm page 3 of 4 accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Touch options . Touch standard additions . A summary of the standard additions procedure will ...

  • Page 328: Iron

    Iron © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 iron_unicell_other_phen_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach off...

  • Page 329: Dr/2400

    Dr/2400 iron irontot_none_other_fvr_eng_ody.Fm page 1 of 4 • if sample contains rust, see interferences on page 2. • digestion is required for total iron determination. • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized wate...

  • Page 330: Iron

    Iron iron page 2 of 4 irontot_none_other_fvr_eng_ody.Fm interferences 5. Fill the rinsed cylinder to the 50-ml mark with sample. 6. Pour the contents of the 50-ml cylinder into one of the flasks. Measure a second 50 ml portion of sample into the graduated cylinder and pour the contents into the seco...

  • Page 331: Iron

    Iron iron irontot_none_other_fvr_eng_ody.Fm page 3 of 4 sample collection, storage and preservation collect samples in acid-washed glass or plastic bottles. To preserve samples, adjust the sample ph to 2 or less with nitric acid (cat. No. 152-49) (about 2 ml per liter). Samples preserved in this man...

  • Page 332: Iron

    Iron © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 irontot_none_other_fvr_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach offi...

  • Page 333: Dr/2400

    Dr/2400 iron, ferrous ironfer_avpp_other_phe_eng_ody.Fm page 1 of 4 • analyze samples as soon as possible to prevent air oxidation of ferrous iron to ferric iron, which is not determined. • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure u...

  • Page 334: Iron, Ferrous

    Iron, ferrous iron, ferrous page 2 of 4 ironfer_avpp_other_phe_eng_ody.Fm 5. Fill a second round sample cell with 25 ml of sample (the blank). 6. When the timer beeps, place the blank into the cell holder. 7. Touch zero . The display will show: 0.00 mg/l fe 2+ 8. Place the prepared sample into the c...

  • Page 335: Iron, Ferrous

    Iron, ferrous iron, ferrous ironfer_avpp_other_phe_eng_ody.Fm page 3 of 4 sample collection, storage and preservation collect samples in plastic or glass bottles. Analyze samples as soon as possible after collection. Accuracy check standard solution method 1. Prepare a ferrous iron stock solution (1...

  • Page 336: Iron, Ferrous

    Iron, ferrous © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 ironfer_avpp_other_phe_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the ...

  • Page 337: Dr/2400

    Dr/2400 iron, total irontot_avpp_other_fvr_eng_ody.Fm page 1 of 6 • digestion is required for determining total iron for epa reporting purposes. See the hach water analysis handbook for digestion procedures. • for more accurate results, determine a reagent blank value for each new lot of reagent. Fo...

  • Page 338: Iron, Total

    Iron, total iron, total page 2 of 6 irontot_avpp_other_fvr_eng_ody.Fm 5. Fill another sample cell (the blank) with 10 ml of sample. 6. When the timer beeps, place the blank into the cell holder. 7. Touch zero . The display will show: 0.00 mg/l fe 8. Place the prepared sample into the cell holder. To...

  • Page 339: Iron, Total

    Iron, total iron, total irontot_avpp_other_fvr_eng_ody.Fm page 3 of 6 interferences 5. Touch the timer icon. Touch ok . A three-minute reaction period will begin. (samples that contain rust should react for at least 5 minutes.) 6. When the timer beeps, place the blank into the cell holder. 7. Touch ...

  • Page 340: Iron, Total

    Iron, total iron, total page 4 of 6 irontot_avpp_other_fvr_eng_ody.Fm sample collection, storage and preservation collect samples in acid-cleaned glass or plastic containers. No acid addition is necessary if analyzing the sample immediately. To preserve samples, adjust the ph to 2 or less with conce...

  • Page 341: Iron, Total

    Iron, total iron, total irontot_avpp_other_fvr_eng_ody.Fm page 5 of 6 3. Touch on . Touch adjust to accept the displayed concentration. If an alternate concentration is used, touch the number in the box to enter the actual concentration, then touch ok . Touch adjust . See section 3.2.4 adjusting the...

  • Page 342

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 343: Dr/2400

    Dr/2400 iron, total irontot_avpp_other_tpt_eng_ody.Fm page 1 of 6 • digestion is required for determining total iron. See the hach water analysis handbook for digestion procedures. • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using de...

  • Page 344: Iron, Total

    Iron, total iron, total page 2 of 6 irontot_avpp_other_tpt_eng_ody.Fm 5. Add the contents of one 10-ml tptz iron reagent powder pillow to the reagent blank. Cap and shake for 30 seconds. 6. When the timer beeps, insert the blank into the cell holder. 7. Touch zero . The display will show: 0.000 mg/l...

  • Page 345: Iron, Total

    Iron, total iron, total irontot_avpp_other_tpt_eng_ody.Fm page 3 of 6 interferences interference tests were performed using an iron concentration of 0.5 mg/l. When interferences occurred, the color formation was inhibited or a precipitate formed. The following do not interfere with the test when pre...

  • Page 346: Iron, Total

    Iron, total iron, total page 4 of 6 irontot_avpp_other_tpt_eng_ody.Fm sample collection, storage and preservation collect samples in acid-washed glass or plastic bottles. To preserve samples, adjust the sample ph to 2 or less with about 2 ml/l nitric acid, acs (cat. No. 152-49). Store preserved samp...

  • Page 347: Iron, Total

    Iron, total iron, total irontot_avpp_other_tpt_eng_ody.Fm page 5 of 6 3. Touch on . Touch adjust to accept the displayed concentration. If an alternate concentration is used, touch the number in the box to enter the actual concentration, then touch ok . Touch adjust . See section 3.2.4 adjusting the...

  • Page 348

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 349: Dr/2400

    Dr/2400 iron, total irontot_pp_other_fmo_eng_ody.Fm page 1 of 4 • digestion is required for total iron determination. See the hach water analysis handbook for digestion procedures. • rinse glassware with a 1:1 hydrochloric acid solution. Rinse again with deionized water. These two steps will remove ...

  • Page 350: Iron, Total

    Iron, total iron, total page 2 of 4 irontot_pp_other_fmo_eng_ody.Fm interferences 5. Fill a clean, round sample cell to the 25-ml mark with prepared sample. Save the remaining 25 ml of prepared sample for step 8. 6. Add the contents of one ferromo iron reagent 2 powder pillow to the sample cell. Swi...

  • Page 351: Iron, Total

    Iron, total iron, total irontot_pp_other_fmo_eng_ody.Fm page 3 of 4 sample collection, storage and preservation collect samples in acid-washed glass or plastic bottles. To preserve samples, adjust the sample ph to 2 or less with hydrochloric acid (about 2 ml per liter) (cat. No. 134-49). Samples pre...

  • Page 352: Iron, Total

    Iron, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 irontot_pp_other_fmo_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach...

  • Page 353: Dr/2400

    Dr/2400 lead lead_none_other_ltk_eng_ody.Fm page 1 of 8 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank adju...

  • Page 354: Lead

    Lead lead page 2 of 8 lead_none_other_ltk_eng_ody.Fm 5. When the timer beeps, use a second 1-ml plastic dropper to add 2.0 ml of ppb-2 fixer solution. Swirl to mix. Note: field samples that have been preserved with nitric acid or samples that have been digested may exceed the buffer capacity of the ...

  • Page 355: Lead

    Lead lead lead_none_other_ltk_eng_ody.Fm page 3 of 8 interferences interference studies were conducted by preparing a known lead solution of approximately 25 µg/l as well as the potential interfering ion. The ion was said to interfere when the resulting lead concentration changed by ±10%. Samples co...

  • Page 356: Lead

    Lead lead page 4 of 8 lead_none_other_ltk_eng_ody.Fm the extractor plunger may be reused for more than one test but should be rinsed between uses. Apparatus and sample preparation because lead is very common to our environment, care must be taken to prevent sample contamination. Follow these steps f...

  • Page 357: Lead

    Lead lead lead_none_other_ltk_eng_ody.Fm page 5 of 8 sampling for lead contamination in household pipes for point-of-use drinking water • the sample should be collected after sitting in pipes with no flow for 8 to 18 hours. • add 10 ml of ppb-1 acid preservative (cat. No. 23685-31) to a one-liter bo...

  • Page 358: Lead

    Lead lead page 6 of 8 lead_none_other_ltk_eng_ody.Fm 4. Open a container of 10-mg/l (10,000 µg/l) lead standard solution. 5. Prepare three sample spikes. Fill three beakers with 100 ml of sample. Use the tensette ® pipet to add 0.1 ml, 0.2 ml, and 0.3 ml of standard, respectively, to each sample and...

  • Page 359: Lead

    Lead lead lead_none_other_ltk_eng_ody.Fm page 7 of 8 method performance precision standard: 750.0 µg/l pb 2+ see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4.5 sensitivity on page 44 for...

  • Page 360

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 361: Dr/2400

    Dr/2400 lead lead_unicell_other_par_eng_ody.Fm page 1 of 4 • adjust ph of preserved samples to between ph 3–5 before analysis. • digest water samples with a ph higher than 9 before analysis. • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel, followed by a dr...

  • Page 362: Lead

    Lead lead page 2 of 4 lead_unicell_other_par_eng_ody.Fm interferences the ions listed in the table have been tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. 9. Touch the timer icon. Touch ok . A second two-minute reaction period will...

  • Page 363: Lead

    Lead lead lead_unicell_other_par_eng_ody.Fm page 3 of 4 total lead, including undissolved lead hydroxide and complexed lead, can only be determined after digesting with the metal prep set, hct 200. Note: the total lead measuring range is 0.24–2.40 mg/l. Sample collection, storage and preservation co...

  • Page 364: Lead

    Lead © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 lead_unicell_other_par_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach offi...

  • Page 365: Dr/2400

    Dr/2400 manganese manganese_pp_high_pox_eng_ody.Fm page 1 of 4 • digestion required. See 2.3 digestion in the laboratory practices section of the dr/2400 procedures manual. • if only dissolved manganese is to be determined, filter the sample before acid addition. • for more accurate results, determi...

  • Page 366: Manganese

    Manganese manganese page 2 of 4 manganese_pp_high_pox_eng_ody.Fm interferences 5. Touch the timer icon. Touch ok . A two-minute reaction period will begin. 6. Fill another round sample cell with 10 ml of sample. 7. When the timer beeps, place the blank into the cell holder. 8. Touch zero . The displ...

  • Page 367: Manganese

    Manganese manganese manganese_pp_high_pox_eng_ody.Fm page 3 of 4 sample collection, storage and preservation collect samples in acid-washed plastic bottles. Do not use glass containers due to possible adsorption of mn to glass. If samples are acidified, adjust the ph to 4–5 with 5.0 n sodium hydroxi...

  • Page 368: Manganese

    Manganese © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 manganese_pp_high_pox_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach ...

  • Page 369: Dr/2400

    Dr/2400 manganese manganese_pp_low_pan_eng_ody.Fm page 1 of 4 • rinse all glassware with 1:1 nitric acid solution. Rinse again with deionized water. • wipe the outside of sample cells before each insertion into the instrument cell holder. Use a damp towel followed by a dry one to remove fingerprints...

  • Page 370: Manganese

    Manganese manganese page 2 of 4 manganese_pp_low_pan_eng_ody.Fm interferences 5. Add 15 drops of alkaline-cyanide reagent solution to each cell. Cap and invert gently to mix. A cloudy solution may form. The turbidity should dissipate after step 6. 6. Add 21 drops of pan indicator solution, 0.1%, to ...

  • Page 371: Manganese

    Manganese manganese manganese_pp_low_pan_eng_ody.Fm page 3 of 4 for samples that contain hardness greater than 300 mg/l caco 3 , add 10 drops of rochelle salt solution (cat. No. 1725-33) to the sample after adding the ascorbic acid powder pillow in step 4. Sample collection, storage and preservation...

  • Page 372: Manganese

    Manganese © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 manganese_pp_low_pan_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach o...

  • Page 373: Dr/2400

    Dr/2400 mercury mercury_pp_other_cvm_eng_ody.Fm page 1 of 12 • perform phase 1 of the procedure in a fume hood. Toxic chlorine or other gases may be produced. • use dedicated digestion glassware and sample cells for this procedure. • determine a reagent blank for each new lot of reagent by running t...

  • Page 374: Mercury

    Mercury mercury page 2 of 12 mercury_pp_other_cvm_eng_ody.Fm 5. Add 7.5 g of potassium permanganate to the sample. Stir until dissolved. Alternatively, add a 10-gram measuring scoop of potassium permanganate to the sample. 6. Cover the flask with a watch glass. Begin heating the sample to a temperat...

  • Page 375: Mercury

    Mercury mercury mercury_pp_other_cvm_eng_ody.Fm page 3 of 12 9. Return the cool, digested sample to the cool, magnetic stirring hot plate. Turn on the stirrer. 10. Using a 0.5-g measuring spoon, add 0.5 g additions of hydroxylamine- hydrochloride until the purple color disappears. Wait 30 seconds af...

  • Page 376: Mercury

    Mercury mercury page 4 of 12 mercury_pp_other_cvm_eng_ody.Fm 5. Connect the power to the vacuum pump and apply vacuum to the mercury absorber column. Draw most of the hgex reagent b into the erlenmeyer flask. 6. Disconnect the vacuum using the quick disconnect when hgex reagent b begins to drip from...

  • Page 377: Mercury

    Mercury mercury mercury_pp_other_cvm_eng_ody.Fm page 5 of 12 13. Touch hach programs . Select program 312 mercury, cold vapor . Touch start . 14. Touch the timer icon. Touch ok . A five-minute reaction period will begin. Let the solution bubble for this period. Air flow rate through the gas washing ...

  • Page 378: Mercury

    Mercury mercury page 6 of 12 mercury_pp_other_cvm_eng_ody.Fm 17. Turn off, or disconnect power to the vacuum pump when the volume in the distilling receiver reaches the 10 ml mark. If necessary, the volume in the distilling receiver may be brought up to 10 ml with hgex reagent b. To avoid low volume...

  • Page 379: Mercury

    Mercury mercury mercury_pp_other_cvm_eng_ody.Fm page 7 of 12 interferences standards were used to prepare a single test solution with the following matrix. A second test solution containing only mercury at the same concentration was prepared as the control. The two solutions were digested then analy...

  • Page 380: Mercury

    Mercury mercury page 8 of 12 mercury_pp_other_cvm_eng_ody.Fm sample collection and preservation collect 1000 ml of sample in an analytically clean, glass or polyethylene terephthalate (pet) container. Add 10 ml of concentrated hydrochloric acid to preserve the sample before sample collection. Fill t...

  • Page 381: Mercury

    Mercury mercury mercury_pp_other_cvm_eng_ody.Fm page 9 of 12 3. Prepare a 0.1-mg/l mercury standard solution by serially diluting a 1000-mg/l mercury standard solution: a. To make a 10.0 mg/l standard, add 1.0 ml of concentrated nitric acid to a 500-ml volumetric flask. Dilute 5.00 ml of a 1000-mg/l...

  • Page 382: Mercury

    Mercury mercury page 10 of 12 mercury_pp_other_cvm_eng_ody.Fm method performance precision standard: 1.40 µg/l hg see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4.5 sensitivity on page 4...

  • Page 383: Mercury

    Mercury mercury mercury_pp_other_cvm_eng_ody.Fm page 11 of 12 the mercuric chloride is eluted off the column and a sensitive indicator is added. The instrument is zeroed using the absorbance peak of the unreacted indicator. A complexing agent is added to break the mercury:indicator complex. The incr...

  • Page 384: Mercury

    Mercury © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 12 of 12 mercury_pp_other_cvm_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach o...

  • Page 385: Dr/2400

    Dr/2400 metolachlor met_none_other_imm_eng_ody.Fm page 1 of 8 this method analyzes for metolachlor in water. Sample calibrators and reagents are added to cuvettes coated with metolachlor-specific antibodies. The color that develops is then measured and compared with the color measurements of the cal...

  • Page 386: Metolachlor

    Metolachlor metolachlor page 2 of 8 met_none_other_imm_eng_ody.Fm 1. Touch single wavelength then touch the λ button. Type in 450 nm and touch ok. 2. Label an antibody cuvette for each calibrator and each sample to be tested. Note: as many as 20 cuvettes may be tested at one time and may comprise an...

  • Page 387: Metolachlor

    Metolachlor metolachlor met_none_other_imm_eng_ody.Fm page 3 of 8 9. At the end of the 20-minute period, discard the contents of all the cuvettes into an appropriate waste container. 10. Wash each cuvette forcefully and thoroughly four times with deionized water. Empty the rinse water into the waste...

  • Page 388: Metolachlor

    Metolachlor metolachlor page 4 of 8 met_none_other_imm_eng_ody.Fm measuring the color 15. Label and fill a zeroing cuvette with deionized water. Wipe the outside of all the cuvettes with a tissue to remove water, smudges, and fingerprints. 16. Install the 1-cm square cell adapter. Note: see section ...

  • Page 389: Metolachlor

    Metolachlor metolachlor met_none_other_imm_eng_ody.Fm page 5 of 8 using the 1-cm microcuvette rack this rack (see figure 1) has been designed specifically to aid in achieving precise and accurate results when using the immunoassay technique to analyze several samples at the same time. Figure 1 the 1...

  • Page 390: Metolachlor

    Metolachlor metolachlor page 6 of 8 met_none_other_imm_eng_ody.Fm interpretation sample #1 — sample reading is less than the readings for both calibrators. Therefore the sample concentration of metolachlor is greater than both 0.5 ppb and 2.0 ppb metolachlor. Sample #2 — sample reading is between th...

  • Page 391: Metolachlor

    Metolachlor metolachlor met_none_other_imm_eng_ody.Fm page 7 of 8 summary of method hach immunoassay tests use antigen/antibody reactions to test for specific organic compounds in water and soil. Metolachlor-specific antibodies, attached to the walls of plastic cuvettes, selectively bind and remove ...

  • Page 392

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 393: Dr/2400

    Dr/2400 molybdenum, molybdate molybdenum_avpp_high_maa_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perfor...

  • Page 394: Molybdenum, Molybdate

    Molybdenum, molybdate molybdenum, molybdate page 2 of 6 molybdenum_avpp_high_maa_eng_ody.Fm 5. Add the contents of one molyver 3 reagent powder pillow. Swirl to mix (the prepared sample). 6. Touch the timer icon. Touch ok . A five-minute reaction period will begin. 7. When the timer beeps, fill a se...

  • Page 395: Molybdenum, Molybdate

    Molybdenum, molybdate molybdenum, molybdate molybdenum_avpp_high_maa_eng_ody.Fm page 3 of 6 1. Touch hach programs . Select program 322 molybdenum hr av . Touch start . 2. Fill a sample cell with 10 ml of sample (the blank). Collect 40 ml of sample in a 50-ml beaker. 3. Add four drops of 0.4 m cdta ...

  • Page 396: Molybdenum, Molybdate

    Molybdenum, molybdate molybdenum, molybdate page 4 of 6 molybdenum_avpp_high_maa_eng_ody.Fm interferences sample collection, storage and preservation collect samples in clean plastic or glass bottles. Adjust the ph to 2 or less with nitric acid (about 2 ml/l). Preserved samples can be stored up to 6...

  • Page 397: Molybdenum, Molybdate

    Molybdenum, molybdate molybdenum, molybdate molybdenum_avpp_high_maa_eng_ody.Fm page 5 of 6 5. Prepare three sample spikes. Fill three mixing cylinders (cat. No. 1896-40) with 30 ml of sample. Use the tensette ® pipet to add 0.2 ml, 0.4 ml, and 0.6 ml of standard, respectively, to each sample and mi...

  • Page 398: Molybdenum, Molybdate

    Molybdenum, molybdate © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 molybdenum_avpp_high_maa_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 co...

  • Page 399: Dr/2400

    Dr/2400 molybdenum, molybdate molybdenum_pp_low_ter_eng_ody.Fm page 1 of 6 • analyze samples immediately after collection. • wipe the outside of sample cells before each insertion into the instrument cell holder. Use a damp towel followed by a dry one to remove fingerprints or other marks. • filter ...

  • Page 400: Molybdenum, Molybdate

    Molybdenum, molybdate molybdenum, molybdate page 2 of 6 molybdenum_pp_low_ter_eng_ody.Fm interferences interferences studies were conducted by preparing a molybdenum standard solution (2-mg/l mo 6+ ) as well as a solution of the potential interfering ion. When the standard solution concentration cha...

  • Page 401: Molybdenum, Molybdate

    Molybdenum, molybdate molybdenum, molybdate molybdenum_pp_low_ter_eng_ody.Fm page 3 of 6 highly buffered samples or extreme sample ph may exceed the buffering capacity of the reagent and require sample pretreatment. Adjust the sample ph to between 3–5 by adding, dropwise, an appropriate amount of ac...

  • Page 402: Molybdenum, Molybdate

    Molybdenum, molybdate molybdenum, molybdate page 4 of 6 molybdenum_pp_low_ter_eng_ody.Fm sample collection, storage and preservation collect samples in glass or plastic bottles. Analyze samples immediately. Accuracy check standard additions method (sample spike) 1. After reading test results, leave ...

  • Page 403: Molybdenum, Molybdate

    Molybdenum, molybdate molybdenum, molybdate molybdenum_pp_low_ter_eng_ody.Fm page 5 of 6 method performance precision standard: 2.00 mg/l mo 6+ see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see secti...

  • Page 404

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 405: Dr/2400

    Dr/2400 nickel nickel_pp_other_hep_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank ad...

  • Page 406: Nickel

    Nickel nickel page 2 of 6 nickel_pp_other_hep_eng_ody.Fm 5. When the timer beeps, add the contents of one nickel 2 reagent powder pillow to the funnel. Stopper and invert to mix. 6. Touch the timer icon. Touch ok . A second five-minute reaction period will begin. 7. When the timer beeps, add 10 ml o...

  • Page 407: Nickel

    Nickel nickel nickel_pp_other_hep_eng_ody.Fm page 3 of 6 interferences cobalt, copper, and iron interferences can be overcome by adding additional nickel 1 reagent powder pillows in step 3. The tolerance limits of these interferences are shown in the following table: a preliminary acid digestion is ...

  • Page 408: Nickel

    Nickel nickel page 4 of 6 nickel_pp_other_hep_eng_ody.Fm accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Touch options . Touch standard additions . A summary of the standard additions procedure will ...

  • Page 409: Nickel

    Nickel nickel nickel_pp_other_hep_eng_ody.Fm page 5 of 6 method performance precision standard: 1.00 mg/l ni see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4.5 sensitivity on page 44 for...

  • Page 410

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 411: Dr/2400

    Dr/2400 nickel nickel_pp_other_pan_eng_ody.Fm page 1 of 6 • cobalt concentration can be determined with the same sample by using program number 110. • wipe the outside of sample cells before each insertion into the instrument cell holder. Use a damp towel followed by a dry one to remove fingerprints...

  • Page 412: Nickel

    Nickel nickel page 2 of 6 nickel_pp_other_pan_eng_ody.Fm 5. Using the plastic dropper provided, add 0.5 ml of 0.3% pan indicator solution to each cell. Cap. Invert several times to mix. 6. Touch the timer icon. Touch ok . A 15-minute reaction period will begin. During color development, the sample s...

  • Page 413: Nickel

    Nickel nickel nickel_pp_other_pan_eng_ody.Fm page 3 of 6 interferences sample collection, storage and preservation collect samples in acid-washed plastic bottles. Adjust the sample ph to 2 or less with nitric acid (cat. No. 2540-49), about 5 ml per liter. Preserved samples can be stored up to six mo...

  • Page 414: Nickel

    Nickel nickel page 4 of 6 nickel_pp_other_pan_eng_ody.Fm 4. Snap the neck off a nickel voluette ® ampule standard, 50-mg/l ni. 5. Prepare three sample spikes. Fill three mixing cylinders (cat. No. 1896-40) with 25 ml of sample. Use the tensette ® pipet to add 0.1 ml, 0.2 ml, and 0.3 ml of standard, ...

  • Page 415: Nickel

    Nickel nickel nickel_pp_other_pan_eng_ody.Fm page 5 of 6 summary of method after buffering the sample and masking any fe 3+ with pyrophosphate, the nickel is reacted with 1-(2-pyridylazo)-2-naphthol indicator. The indicator forms complexes with most metals present. After color development, edta is a...

  • Page 416

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 417: Dr/2400

    Dr/2400 nickel nickel_unicell_other_dimeth_eng_ody.Fm page 1 of 4 • undissolved or complexed nickel can only be determined after digestion with the metal prep set (hct 200). • adjust ph of preserved samples to between ph 3–8 before analysis. • the outside of the vial must be clean before placing it ...

  • Page 418: Nickel

    Nickel nickel page 2 of 4 nickel_unicell_other_dimeth_eng_ody.Fm interferences the ions listed in the table have been tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. Sample collection, storage, and preservation collect samples in aci...

  • Page 419: Nickel

    Nickel nickel nickel_unicell_other_dimeth_eng_ody.Fm page 3 of 4 accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Touch options . Touch standard additions . A summary of the standard additions procedu...

  • Page 420: Nickel

    Nickel © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 nickel_unicell_other_dimeth_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the ha...

  • Page 421: Dr/2400

    Dr/2400 nitrate nitrate_avpp_hr_cdr_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank a...

  • Page 422: Nitrate

    Nitrate nitrate page 2 of 6 nitrate_avpp_hr_cdr_eng_ody.Fm 5. When the timer beeps, touch the timer icon. Touch ok . A five-minute reaction period will begin. An amber color will develop if nitrate is present. 6. When the timer beeps, fill a second round sample cell with 10 ml of sample (this is the...

  • Page 423: Nitrate

    Nitrate nitrate nitrate_avpp_hr_cdr_eng_ody.Fm page 3 of 6 1. Touch hach programs . Select program 361 n, nitrate hr av . Touch start . 2. Collect at least 40 ml of sample in a 50-ml beaker. 3. Fill a nitraver 5 nitrate accuvac ® ampul with sample. Keep the tip immersed while the ampule fills comple...

  • Page 424: Nitrate

    Nitrate nitrate page 4 of 6 nitrate_avpp_hr_cdr_eng_ody.Fm interferences sample collection, storage, and preservation more reliable results are obtained when samples are analyzed as soon as possible after collection. If prompt analysis is impossible, store samples in clean plastic or glass bottles f...

  • Page 425: Nitrate

    Nitrate nitrate nitrate_avpp_hr_cdr_eng_ody.Fm page 5 of 6 accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. Verify the chemical form. 2. Touch options . Touch standard additions . A summary of the standa...

  • Page 426: Nitrate

    Nitrate © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 nitrate_avpp_hr_cdr_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach offi...

  • Page 427: Dr/2400

    Dr/2400 nitrate nitrate_avpp_mid_cdr_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank ...

  • Page 428: Nitrate

    Nitrate nitrate page 2 of 6 nitrate_avpp_mid_cdr_eng_ody.Fm 5. When the timer beeps, touch the timer icon. Touch ok . A five-minute reaction period will begin. An amber color will develop if nitrate is present. 6. When the timer beeps, fill a second round sample cell with 10 ml of sample (this is th...

  • Page 429: Nitrate

    Nitrate nitrate nitrate_avpp_mid_cdr_eng_ody.Fm page 3 of 6 1. Touch hach programs . Select program 359 n, nitrate mr av . Touch start . 2. Collect at least 40 ml of sample in a 50-ml beaker. 3. Fill a nitraver 5 nitrate accuvac ® ampul with sample. Keep the tip immersed while the ampule fills compl...

  • Page 430: Nitrate

    Nitrate nitrate page 4 of 6 nitrate_avpp_mid_cdr_eng_ody.Fm interferences sample collection, storage and preservation most reliable results are obtained when samples are analyzed as soon as possible after collection. If prompt analysis is impossible, store samples in clean plastic or glass bottles f...

  • Page 431: Nitrate

    Nitrate nitrate nitrate_avpp_mid_cdr_eng_ody.Fm page 5 of 6 before analysis, warm the sample to room temperature and adjust the ph to 7 with 5.0 n sodium hydroxide standard solution (cat. No. 2450-53). Do not use mercury compounds as preservatives. Correct the test result for volume additions by div...

  • Page 432: Nitrate

    Nitrate © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 nitrate_avpp_mid_cdr_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach off...

  • Page 433: Dr/2400

    Dr/2400 nitrate nitrate_pp_low_cdr_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank ad...

  • Page 434: Nitrate

    Nitrate nitrate page 2 of 6 nitrate_pp_low_cdr_eng_ody.Fm 5. When the timer beeps, touch the timer icon again. Touch ok . A 2-minute reaction period will begin. 6. When the timer beeps, carefully pour 10 ml of the sample into a clean, round sample cell. Do not transfer any cadmium particles to the s...

  • Page 435: Nitrate

    Nitrate nitrate nitrate_pp_low_cdr_eng_ody.Fm page 3 of 6 interferences sample collection, storage and preservation more reliable results are obtained when samples are analyzed as soon as possible after collection. If prompt analysis is impossible, store samples in clean plastic or glass bottles for...

  • Page 436: Nitrate

    Nitrate nitrate page 4 of 6 nitrate_pp_low_cdr_eng_ody.Fm accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. Verify the chemical form. 2. Touch options . Touch standard additions . A summary of the standar...

  • Page 437: Nitrate

    Nitrate nitrate nitrate_pp_low_cdr_eng_ody.Fm page 5 of 6 method performance precision standard: 0.2 mg/l no 3 – –n see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4.5 sensitivity on page...

  • Page 438

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 439: Dr/2400

    Dr/2400 nitrate nitrate_tnt_high_cha_eng_ody.Fm page 1 of 4 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water (nitrate-free) in place of the sample. Subtract the reagent blank value from the final results or perform a...

  • Page 440: Nitrate

    Nitrate nitrate page 2 of 4 nitrate_tnt_high_cha_eng_ody.Fm interferences 5. Touch zero . The display will show: 0.0 mg/l no 3 – –n 6. Remove the vial from the instrument. Using a funnel, add the contents of one nitraver x reagent b powder pillow to the vial. 7. Cap and invert ten times to mix (this...

  • Page 441: Nitrate

    Nitrate nitrate nitrate_tnt_high_cha_eng_ody.Fm page 3 of 4 sample collection, preservation, and storage collect samples in clean plastic or glass bottles. Store at 4 °c (39 °f) or lower if the sample is to be analyzed within 24 to 48 hours. Warm to room temperature before running the test. For long...

  • Page 442: Nitrate

    Nitrate © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 nitrate_tnt_high_cha_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach off...

  • Page 443: Dr/2400

    Dr/2400 nitrate nitrate_unicell_dimeth_eng_ody.Fm page 1 of 4 • adjust the ph of preserved samples to between ph 3–10 before testing. • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel, followed by a dry one. • use the standard solution or standard addition m...

  • Page 444: Nitrate

    Nitrate nitrate page 2 of 4 nitrate_unicell_dimeth_eng_ody.Fm interferences the ions and substances listed in the following tables have been individually tested up to the given concentrations. Cumulative effects and the influence of other ions and substances have not been evaluated. 5. Cap the sampl...

  • Page 445: Nitrate

    Nitrate nitrate nitrate_unicell_dimeth_eng_ody.Fm page 3 of 4 sample collection, preservation, and storage analyze samples within three hours after collection for best results. Collect samples in clean plastic or glass bottles. Store in a cool place. Warm to room temperature before performing the te...

  • Page 446: Nitrate

    Nitrate © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 nitrate_unicell_dimeth_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach o...

  • Page 447: Dr/2400

    Dr/2400 nitrite nitrite_avpp_low_daz_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank ...

  • Page 448: Nitrite

    Nitrite nitrite page 2 of 6 nitrite_avpp_low_daz_eng_ody.Fm 5. When the timer beeps, fill a second sample cell with 10 ml of sample (this is the blank). 6. Wipe the blank and place it into the cell holder. 7. Touch zero . The display will show: 0.000 mg/l no 2 – –n 8. Wipe the prepared sample and pl...

  • Page 449: Nitrite

    Nitrite nitrite nitrite_avpp_low_daz_eng_ody.Fm page 3 of 6 5. Touch the timer icon. Touch ok . A 20-minute reaction period will begin. 6. When the timer beeps, fill a sample cell with at least 10 ml of sample (this is the blank). 7. Wipe the blank and place it into the cell holder. 8. Touch zero . ...

  • Page 450: Nitrite

    Nitrite nitrite page 4 of 6 nitrite_avpp_low_daz_eng_ody.Fm interferences sample collection, storage, and preservation collect samples in clean plastic or glass bottles. Store at 4 °c (30 °f) or lower if the sample is to be analyzed within 24 to 48 hours. Warm to room temperature before running the ...

  • Page 451: Nitrite

    Nitrite nitrite nitrite_avpp_low_daz_eng_ody.Fm page 5 of 6 sensitivity see section 3.4.5 sensitivity on page 44 for more information. Summary of method nitrite in the sample reacts with sulfanilic acid to form an intermediate diazonium salt. This couples with chromotropic acid to produce a pink col...

  • Page 452

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 453: Dr/2400

    Dr/2400 nitrite nitrite_pp_high_fes_eng_ody.Fm page 1 of 4 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank a...

  • Page 454: Nitrite

    Nitrite nitrite page 2 of 4 nitrite_pp_high_fes_eng_ody.Fm interferences this test does not measure nitrates nor is it applicable to glycol-based samples. Dilute glycol-based samples and follow the low range nitrite procedure. Sample collection, storage, and preservation collect samples in clean pla...

  • Page 455: Nitrite

    Nitrite nitrite nitrite_pp_high_fes_eng_ody.Fm page 3 of 4 accuracy check standard solution method preparing nitrite standards is difficult. A standard should be prepared by a trained chemist. Hach recommends using the standard preparation instructions in standard methods for the examination of wate...

  • Page 456

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 457: Dr/2400

    Dr/2400 nitrite nitrite_tnt_low_daz_eng_ody.Fm page 1 of 4 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank a...

  • Page 458: Nitrite

    Nitrite nitrite page 2 of 4 nitrite_tnt_low_daz_eng_ody.Fm interferences sample collection, storage, and preservation collect samples in clean plastic or glass bottles. Store at 4 °c (30 °f) or lower if the sample is to be analyzed within 24 to 48 hours. Warm to room temperature before running the t...

  • Page 459: Nitrite

    Nitrite nitrite nitrite_tnt_low_daz_eng_ody.Fm page 3 of 4 accuracy check standard solution method preparing nitrite standards is difficult. A standard should be prepared by a trained chemist. Hach recommends using the standard preparation instructions in standard methods for the examination of wate...

  • Page 460

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 461: Dr/2400

    Dr/2400 nitrite nitrite_unicell_low_daz_eng_ody.Fm page 1 of 4 • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel, followed by a dry one. • use the standard solution or standard addition method for a proof of accuracy. (see accuracy check). • see 3.2.4 on pag...

  • Page 462: Nitrite

    Nitrite nitrite page 2 of 4 nitrite_unicell_low_daz_eng_ody.Fm interferences the ions listed in the table have been tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. Chromium (vi) ions interfere. Copper (ii) ions interfere even at conc...

  • Page 463: Nitrite

    Nitrite nitrite nitrite_unicell_low_daz_eng_ody.Fm page 3 of 4 accuracy check standard solution method prepare a 0.25-mg/l no 2 – –n standard solution by pipetting 1.00 ml of 250-mg/l no 2 – –n standard into a 1000-ml volumetric flask. Dilute to the mark with deionized water. Stopper and invert to m...

  • Page 464

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 465: Dr/2400

    Dr/2400 nitrogen, ammonia nitrogenamm_none_other_nes_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform ...

  • Page 466: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia page 2 of 6 nitrogenamm_none_other_nes_eng_ody.Fm 5. Add three drops of polyvinyl alcohol dispersing agent to each cylinder. Stopper and invert several times to mix. 6. Pipet 1.0 ml of nessler reagent into each cylinder. Stopper and invert several times to mix. 7....

  • Page 467: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia nitrogenamm_none_other_nes_eng_ody.Fm page 3 of 6 interferences sample collection, storage, and preservation collect samples in clean glass or plastic bottles. If chlorine is present, add one drop of 0.1 n sodium thiosulfate (cat. No. 323-32) for each 0.3 mg/l cl ...

  • Page 468: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia page 4 of 6 nitrogenamm_none_other_nes_eng_ody.Fm 7. After completing the sequence, the display will show the extrapolated concentration value and the “best-fit” line through the standard additions data points, accounting for matrix interferences. See section 3.2....

  • Page 469: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia nitrogenamm_none_other_nes_eng_ody.Fm page 5 of 6 5. Turn on the heater power switch. Set the stir control to 5 and the heat control to 10. Turn on the water and adjust to maintain a constant flow through the condenser. 6. Turn off the heater after collecting 150 ...

  • Page 470

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 471: Dr/2400

    Dr/2400 nitrogen, ammonia nitrogenamm_pp_other_sal_eng_ody.Fm page 1 of 4 nitrogen, ammonia method 8155 salicylate method* * adapted from clin. Chim. Acta., 14, 403 (1966) • a green color will develop if ammonia nitrogen is present. Powder pillows (0.01 to 0.50 mg/l nh 3 –n) scope and application: f...

  • Page 472: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia page 2 of 4 nitrogenamm_pp_other_sal_eng_ody.Fm interferences sample collection, storage, and preservation collect samples in clean plastic or glass bottles. Most reliable results are obtained when samples are analyzed as soon as possible after collection. If chlo...

  • Page 473: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia nitrogenamm_pp_other_sal_eng_ody.Fm page 3 of 4 for longer storage, adjust the ph to 2 or less with concentrated (about 2 ml per liter) sulfuric acid (cat. No. 979-49). Store samples at 4 °c or less. Samples preserved in this manner can be stored up to 28 days. Ju...

  • Page 474: Nitrogen, Ammonia

    Nitrogen, ammonia © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 nitrogenamm_pp_other_sal_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contac...

  • Page 475: Dr/2400

    Dr/2400 nitrogen, ammonia nitrogenamm_tnt_high_sal_eng_ody.Fm page 1 of 6 • small sample sizes (such as 0.1 ml) may not be representative of the entire sample. Mix the sample well before testing or repeat the test, sampling from different portions of the sample. • wipe the outside of sample cells be...

  • Page 476: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia page 2 of 6 nitrogenamm_tnt_high_sal_eng_ody.Fm 5. Add the contents of one ammonia cyanurate reagent powder pillow to each vial. 6. Cap the vials tightly and shake thoroughly to dissolve the powder. 7. Touch the timer icon. Touch ok . A 20-minute reaction period w...

  • Page 477: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia nitrogenamm_tnt_high_sal_eng_ody.Fm page 3 of 6 interferences in some lab environments, airborne cross contamination of the blank is possible. Complete preparation of the blank before opening or handling any samples or standards to avoid transfer of ammonia. If sa...

  • Page 478: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia page 4 of 6 nitrogenamm_tnt_high_sal_eng_ody.Fm accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Touch options . Touch standard additions . A summary of the standar...

  • Page 479: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia nitrogenamm_tnt_high_sal_eng_ody.Fm page 5 of 6 method performance precision standard: 10.0 mg/l nh 3 –n see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section ...

  • Page 480

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 481: Dr/2400

    Dr/2400 nitrogen, ammonia nitrogenamm_tnt_low_sal_eng_ody.Fm page 1 of 6 • the ammonia salicylate reagent contains sodium nitroferricyanide. Cyanide solutions are regulated as hazardous wastes by the federal rcra. Collect cyanide solutions for disposal as reactive (d001) waste. Be sure cyanide solut...

  • Page 482: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia page 2 of 6 nitrogenamm_tnt_low_sal_eng_ody.Fm interferences 5. Add the contents of one ammonia cyanurate reagent powder pillow to each vial. 6. Cap the vials tightly and shake thoroughly to dissolve the powder. 7. Touch the timer icon. Touch ok . A 20-minute reac...

  • Page 483: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia nitrogenamm_tnt_low_sal_eng_ody.Fm page 3 of 6 sample collection, storage, and preservation collect samples in clean plastic or glass bottles. Best results are obtained with immediate analysis. If chlorine is known to be present, add one drop of 0.1 n sodium thios...

  • Page 484: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia page 4 of 6 nitrogenamm_tnt_low_sal_eng_ody.Fm 7. After completing the sequence, touch graph to view the best-fit line through the standard additions data points, accounting for matrix interferences. Touch view: fit , then select ideal line and touch ok to view th...

  • Page 485: Nitrogen, Ammonia

    Nitrogen, ammonia nitrogen, ammonia nitrogenamm_tnt_low_sal_eng_ody.Fm page 5 of 6 required reagents quantity required description per test unit cat. No. Low range test ’n tube™ nitrogen-ammonia amver™ reagent set..................... 25 tests ...............26045-45 required apparatus adapter, 16-m...

  • Page 486

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 487: Dr/2400

    Dr/2400 nitrogen, ammonium nitrogenamm_unicell_sal_eng_ody.Fm page 1 of 4 • adjust the ph of preserved samples to between ph 4–9 before testing. • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel, followed by a dry one. • use the standard solution or standard...

  • Page 488: Nitrogen, Ammonium

    Nitrogen, ammonium nitrogen, ammonium page 2 of 4 nitrogenamm_unicell_sal_eng_ody.Fm interferences the ions listed in the following table have been individually tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. Sample, collection, stor...

  • Page 489: Nitrogen, Ammonium

    Nitrogen, ammonium nitrogen, ammonium nitrogenamm_unicell_sal_eng_ody.Fm page 3 of 4 accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Touch options . Touch standard additions . A summary of the standa...

  • Page 490: Nitrogen, Ammonium

    Nitrogen, ammonium © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 nitrogenamm_unicell_sal_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contac...

  • Page 491: Dr/2400

    Dr/2400 nitrogen, ammonium nitrogenamm_unicell_low_sal_eng_ody.Fm page 1 of 4 • adjust the ph of preserved samples to between ph 4–9 before testing. • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel, followed by a dry one. • use the standard solution or stan...

  • Page 492: Nitrogen, Ammonium

    Nitrogen, ammonium nitrogen, ammonium page 2 of 4 nitrogenamm_unicell_low_sal_eng_ody.Fm interferences the ions listed in the following table have been individually tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. 5. Touch the timer i...

  • Page 493: Nitrogen, Ammonium

    Nitrogen, ammonium nitrogen, ammonium nitrogenamm_unicell_low_sal_eng_ody.Fm page 3 of 4 sample, collection, storage, and preservation analyze samples within 3 hours after collection for best results. Collect samples in clean plastic or glass bottles. To preserve samples for longer periods, add 1 ml...

  • Page 494: Nitrogen, Ammonium

    Nitrogen, ammonium © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 nitrogenamm_unicell_low_sal_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 co...

  • Page 495: Dr/2400

    Dr/2400 nitrogen, total nitrogentot_tnt_high_psd_eng_ody.Fm page 1 of 6 • digestion is required for determining total nitrogen. The digestion procedure is included below. • place a safety shield in front of the cod reactor to prevent injury if splattering occurs. • this test is technique-sensitive. ...

  • Page 496: Nitrogen, Total

    Nitrogen, total nitrogen, total page 2 of 6 nitrogentot_tnt_high_psd_eng_ody.Fm 5. Place the vials in the reactor. Heat for exactly 30 minutes. 6. Using finger cots, immediately remove the hot vials from the reactor. Cool the vials to room temperature. 7. Touch hach programs . Select program 395 n, ...

  • Page 497: Nitrogen, Total

    Nitrogen, total nitrogen, total nitrogentot_tnt_high_psd_eng_ody.Fm page 3 of 6 blanks for colorimetric measurement the reagent blank may be used repeatedly for measurements using the same lots of reagents. Store the reagent blank in the dark at room temperature (18–25 °c) for a maximum of seven day...

  • Page 498: Nitrogen, Total

    Nitrogen, total nitrogen, total page 4 of 6 nitrogentot_tnt_high_psd_eng_ody.Fm hach chemists tested this chemistry on standard nitrogen solutions prepared from the following compounds and obtained 95% recovery: ammonium chloride or nicotinic-ptsa spikes in domestic influent, effluent and the astm s...

  • Page 499: Nitrogen, Total

    Nitrogen, total nitrogen, total nitrogentot_tnt_high_psd_eng_ody.Fm page 5 of 6 the percent recovery should be: hach analysts have found ammonia-ptsa to be the most difficult to digest. Other compounds may yield different percent recoveries. Standard additions method (sample spike) 1. After reading ...

  • Page 500: Nitrogen, Total

    Nitrogen, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 nitrogentot_tnt_high_psd_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact ...

  • Page 501: Dr/2400

    Dr/2400 nitrogen, total nitrogentot_tnt_other_psd_eng_ody.Fm page 1 of 8 • digestion is required for determining total nitrogen. The digestion procedure is included below. • place a safety shield in front of the cod reactor to prevent injury if splattering occurs. • this test is technique-sensitive....

  • Page 502: Nitrogen, Total

    Nitrogen, total nitrogen, total page 2 of 8 nitrogentot_tnt_other_psd_eng_ody.Fm 5. Place the vials in the reactor. Heat for exactly 30 minutes. 6. Using finger cots, immediately remove the hot vials from the reactor. Cool the vials to room temperature. 7. Touch hach programs . Select program 350 n,...

  • Page 503: Nitrogen, Total

    Nitrogen, total nitrogen, total nitrogentot_tnt_other_psd_eng_ody.Fm page 3 of 8 blanks for colorimetric measurement the reagent blank may be used up to seven days for measurements using the same lots of reagents. Store it in the dark at room temperature (18–25 °c). If a small amount of white floc a...

  • Page 504: Nitrogen, Total

    Nitrogen, total nitrogen, total page 4 of 8 nitrogentot_tnt_other_psd_eng_ody.Fm interferences the substances in table 1 have been tested and found not to interfere up to the indicated levels (in mg/l). Interfering substances the resulted in a concentration change of ±10% appear in table 2. Hach che...

  • Page 505: Nitrogen, Total

    Nitrogen, total nitrogen, total nitrogentot_tnt_other_psd_eng_ody.Fm page 5 of 8 sample collection, storage and preservation collect samples in clean plastic or glass bottles. Best results are obtained with immediate analysis. Preserve the sample by reducing the ph to 2 or less with at least 2 ml of...

  • Page 506: Nitrogen, Total

    Nitrogen, total nitrogen, total page 6 of 8 nitrogentot_tnt_other_psd_eng_ody.Fm standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. Verify the chemical form. 2. Touch options . Touch standard additions . A summary of the...

  • Page 507: Nitrogen, Total

    Nitrogen, total nitrogen, total nitrogentot_tnt_other_psd_eng_ody.Fm page 7 of 8 method performance precision standard: 10 mg/l nh 3 –n see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4.5...

  • Page 508

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 509: Dr/2400

    Dr/2400 nitrogen, total nitrogentot_unicell_other_psd_eng_ody.Fm page 1 of 6 • warm the sample to room temperature and adjust ph between ph 4–9 with 5.0 n sodium hydroxide standard solution before testing. • do not use mercury compounds as preservatives. • clean the outside of the vial before placin...

  • Page 510: Nitrogen, Total

    Nitrogen, total nitrogen, total page 2 of 6 nitrogentot_unicell_other_psd_eng_ody.Fm 5. Heat the reaction tube in the reactor block at 100 °c for 60 minutes. 6. After the heating period, remove the tube from the reactor block and place it in a cooling rack. 7. When the reaction tube has cooled to ro...

  • Page 511: Nitrogen, Total

    Nitrogen, total nitrogen, total nitrogentot_unicell_other_psd_eng_ody.Fm page 3 of 6 interferences the ions listed in the table have been tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. Ions may interfere when present in conditions e...

  • Page 512: Nitrogen, Total

    Nitrogen, total nitrogen, total page 4 of 6 nitrogentot_unicell_other_psd_eng_ody.Fm 4. Prepare three sample spikes by filling three mixing cylinders with 50 ml of sample. Use a pipet to add 0.2, 0.4, and 0.6 ml of 1000-mg/l no 3 –n standard, respectively, to each sample and mix thoroughly. 5. Analy...

  • Page 513: Nitrogen, Total

    Nitrogen, total nitrogen, total nitrogentot_unicell_other_psd_eng_ody.Fm page 5 of 6 summary of method bound nitrogen, both inorganically and organically bound, is oxidized to nitrate by digestion with peroxodisulphate. The nitrate ions react with 2,6-dimethylphenol in a solution of sulphuric and ph...

  • Page 514

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 515: Dr/2400

    Dr/2400 nitrogen, total inorganic nitrogenti_tnt_other_ttr_eng_ody.Fm page 1 of 6 • this test requires a centrifuge. • for safety, wear gloves while breaking ampules. • wipe the outside of sample cells before each insertion into the instrument cell holder. Use a damp towel followed by a dry one to r...

  • Page 516: Nitrogen, Total Inorganic

    Nitrogen, total inorganic nitrogen, total inorganic page 2 of 6 nitrogenti_tnt_other_ttr_eng_ody.Fm 5. Pour the contents of one total inorganic nitrogen reductant ampule into the vial containing the sample. Pour the contents of another total inorganic nitrogen reductant ampule into the vial containi...

  • Page 517: Nitrogen, Total Inorganic

    Nitrogen, total inorganic nitrogen, total inorganic nitrogenti_tnt_other_ttr_eng_ody.Fm page 3 of 6 interferences the following ions may interfere when present: the following do not interfere below the levels listed: 13. Touch the timer icon. Touch ok . A 20-minute reaction period will begin. 14. In...

  • Page 518: Nitrogen, Total Inorganic

    Nitrogen, total inorganic nitrogen, total inorganic page 4 of 6 nitrogenti_tnt_other_ttr_eng_ody.Fm sample collection, preservation, and storage collect samples in clean plastic or glass bottles. Best results are obtained with immediate analysis. If chlorine is known to be present, add one drop of 0...

  • Page 519: Nitrogen, Total Inorganic

    Nitrogen, total inorganic nitrogen, total inorganic nitrogenti_tnt_other_ttr_eng_ody.Fm page 5 of 6 method performance the total inorganic nitrogen test is designed to provide an estimate of the total nitrite, nitrate, and ammonia nitrogen load present in a water or wastewater sample. This test is m...

  • Page 520: Nitrogen, Total Inorganic

    Nitrogen, total inorganic © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 nitrogenti_tnt_other_ttr_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-422...

  • Page 521: Dr/2400

    Dr/2400 nitrogen, total kjeldahl nitrogentk_none_other_nes_eng_ody.Fm page 1 of 6 ó • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or ...

  • Page 522: Nitrogen, Total Kjeldahl

    Nitrogen, total kjeldahl nitrogen, total kjeldahl page 2 of 6 nitrogentk_none_other_nes_eng_ody.Fm 5. If the aliquot is less than 1 ml, proceed to step 6. If it is greater than 1 ml, add drops of 8.0 n koh to each cylinder until the first flash of blue color appears. Stopper and invert the cylinder ...

  • Page 523: Nitrogen, Total Kjeldahl

    Nitrogen, total kjeldahl nitrogen, total kjeldahl nitrogentk_none_other_nes_eng_ody.Fm page 3 of 6 13. When the timer beeps, pour the contents of each cylinder into separate 10-ml sample cells. 14. Wipe the blank and place it into the cell holder. 15. Touch zero . The display will show: 0 mg/l tkn 1...

  • Page 524: Nitrogen, Total Kjeldahl

    Nitrogen, total kjeldahl nitrogen, total kjeldahl page 4 of 6 nitrogentk_none_other_nes_eng_ody.Fm sample collection, storage, and preservation collect samples in clean glass or plastic containers. Adjust the ph to 2 or less with sulfuric acid (cat. No. 979-49) (about 2 ml per liter) and cool to 4 °...

  • Page 525: Nitrogen, Total Kjeldahl

    Nitrogen, total kjeldahl nitrogen, total kjeldahl nitrogentk_none_other_nes_eng_ody.Fm page 5 of 6 method performance precision standard: 120 mg/l nh 3 –n see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivit...

  • Page 526

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 527: Dr/2400

    Dr/2400 organic carbon, total organiccarbontotal_other_high_dir_eng_ody.Fm page 1 of 6 • place a safety shield in front of the cod reactor to prevent injury if splattering occurs. • a reagent blank is required for each series of samples. • wipe the outside of sample cells before each insertion into ...

  • Page 528: Organic Carbon, Total

    Organic carbon, total organic carbon, total page 2 of 6 organiccarbontotal_other_high_dir_eng_ody.Fm 5. Label two high range acid digestion vials sample and reagent blank. 6. Use a funnel to add the contents of one toc persulfate powder pillow to each acid digestion vial (colorless liquid). 7. Use a...

  • Page 529: Organic Carbon, Total

    Organic carbon, total organic carbon, total organiccarbontotal_other_high_dir_eng_ody.Fm page 3 of 6 interferences the following have been tested for interference and found not to interfere up to the indicated levels: 13. Install the 16-mm cell adapter. Note: see section 2.6 sample cell adapters in ...

  • Page 530: Organic Carbon, Total

    Organic carbon, total organic carbon, total page 4 of 6 organiccarbontotal_other_high_dir_eng_ody.Fm if the sample contains greater than 1000 mg/l caco 3 alkalinity, lower the sample ph to less than 7 before testing by adding sulfuric acid solution (cat. No. 2449-32). Most sample turbidity is either...

  • Page 531: Organic Carbon, Total

    Organic carbon, total organic carbon, total organiccarbontotal_other_high_dir_eng_ody.Fm page 5 of 6 method performance precision see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4.5 sensi...

  • Page 532: Organic Carbon, Total

    Organic carbon, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 organiccarbontotal_other_high_dir_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-22...

  • Page 533: Dr/2400

    Dr/2400 organic carbon, total organiccarbontotal_other_mid_dir_eng_ody.Fm page 1 of 6 • place a safety shield in front of the cod reactor to prevent injury if splattering occurs. • a reagent blank is required for each series of samples. • wipe the outside of sample cells before each insertion into t...

  • Page 534: Organic Carbon, Total

    Organic carbon, total organic carbon, total page 2 of 6 organiccarbontotal_other_mid_dir_eng_ody.Fm 5. Label two mid range acid digestion vials sample and reagent blank. 6. Use a funnel to add the contents of one toc persulfate powder pillow to each acid digestion vial (colorless liquid). 7. Use a t...

  • Page 535: Organic Carbon, Total

    Organic carbon, total organic carbon, total organiccarbontotal_other_mid_dir_eng_ody.Fm page 3 of 6 interferences the following have been tested for interference and found not to interfere up to the indicated levels: 13. Install the 16-mm cell adapter. Note: see section 2.6 sample cell adapters in t...

  • Page 536: Organic Carbon, Total

    Organic carbon, total organic carbon, total page 4 of 6 organiccarbontotal_other_mid_dir_eng_ody.Fm if the sample contains greater than 1000 mg/l caco 3 alkalinity, lower the sample ph to less than 7 before testing by adding sulfuric acid solution (cat. No. 2449-32). Most sample turbidity is either ...

  • Page 537: Organic Carbon, Total

    Organic carbon, total organic carbon, total organiccarbontotal_other_mid_dir_eng_ody.Fm page 5 of 6 method performance precision see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section 3.4.5 sensit...

  • Page 538: Organic Carbon, Total

    Organic carbon, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 organiccarbontotal_other_mid_dir_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227...

  • Page 539: Dr/2400

    Dr/2400 organic carbon, total organiccarbontotal_other_low_dir_eng_ody.Fm page 1 of 6 • place a safety shield in front of the cod reactor to prevent injury if splattering occurs. • a reagent blank is required for each series of samples. • wipe the outside of sample cells before each insertion into t...

  • Page 540: Organic Carbon, Total

    Organic carbon, total organic carbon, total page 2 of 6 organiccarbontotal_other_low_dir_eng_ody.Fm 5. Label two low range acid digestion vials “sample” and “reagent blank”. 6. Use a funnel to add the contents of one toc persulfate powder pillow to each acid digestion vial (colorless liquid). 7. Use...

  • Page 541: Organic Carbon, Total

    Organic carbon, total organic carbon, total organiccarbontotal_other_low_dir_eng_ody.Fm page 3 of 6 interferences the following have been tested for interference and found not to interfere up to the indicated levels: 13. Wipe the reagent blank with a damp towel, followed by a dry one, to remove fing...

  • Page 542: Organic Carbon, Total

    Organic carbon, total organic carbon, total page 4 of 6 organiccarbontotal_other_low_dir_eng_ody.Fm if the sample contains greater than 600 mg/l caco 3 alkalinity, lower the sample ph to less than 7 before testing by adding sulfuric acid solution (cat. No. 2449-32). Most sample turbidity is either d...

  • Page 543: Organic Carbon, Total

    Organic carbon, total organic carbon, total organiccarbontotal_other_low_dir_eng_ody.Fm page 5 of 6 5. Continue the test starting at step 8 on page 2 of this procedure. 6. The mg/l c concentration should increase by 5.0 mg/l for each 0.1 ml increment. See section 3.2.2 standard additions on page 32 ...

  • Page 544: Organic Carbon, Total

    Organic carbon, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 organiccarbontotal_other_low_dir_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227...

  • Page 545: Dr/2400

    Dr/2400 oxygen demand, chemical oxygencod_none_mid_rcd_eng_ody.Fm page 1 of 8 • some of the chemicals and apparatus used in this procedure may be hazardous to the health and safety of the user if inappropriately handled or accidentally misused. Please read all warnings and refer to waste management ...

  • Page 546: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical page 2 of 8 oxygencod_none_mid_rcd_eng_ody.Fm 5. Hold one vial at a 45-degree angle. Use a clean volumetric pipet to add 2.00 ml of sample to the vial. This is the prepared sample. Note: use a tensette pipet to add 0.20 ml for the 200– 15,000 mg/l rang...

  • Page 547: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical oxygencod_none_mid_rcd_eng_ody.Fm page 3 of 8 1. Touch hach programs . Select program 430 cod lr (low range) or 435 cod hr (high range/high range plus). Touch start . 2. Clean the outside of the vials with a damp towel followed by a dry one to remove f...

  • Page 548: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical page 4 of 8 oxygencod_none_mid_rcd_eng_ody.Fm blanks for colorimetric determination the blank may be used repeatedly for measurements using the same lot of vials. Store it in the dark. Monitor decomposition by measuring the absorbance at the appropriat...

  • Page 549: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical oxygencod_none_mid_rcd_eng_ody.Fm page 5 of 8 accuracy check standard solution method 1. Check the accuracy of the 3 to 150 mg/l range with a 100 mg/l standard. Prepare by dissolving 85 mg of dried (120 °c, overnight) potassium acid phthalate (khp) in ...

  • Page 550: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical page 6 of 8 oxygencod_none_mid_rcd_eng_ody.Fm method performance precision standard: 80 mg/l cod (low range), 800 mg/l cod (high range), and 10,000mg/l cod (high range plus) see section 3.4.3 precision on page 44 for more information, or if the standar...

  • Page 551: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical oxygencod_none_mid_rcd_eng_ody.Fm page 7 of 8 required reagents quantity required description per test unit cat. No. Select the appropriate cod digestion reagent vial: low range, 0 to 150 mg/l cod ..................................................... 1...

  • Page 552

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 553: Dr/2400

    Dr/2400 oxygen demand, chemical oxygencod_other_other_md3_eng_ody.Fm page 1 of 8 • use this method if the sample contains chloride. To determine if the sample contains chloride, use quantab ® titrator strips for low range chloride (cat. No. 27449-40). • if the sample cod is expected to exceed 1000 m...

  • Page 554: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical page 2 of 8 oxygencod_other_other_md3_eng_ody.Fm calculating the multiplication factor all dilutions require that the ratio of sample to sulfuric acid remain at 9:1. For other dilutions that are not listed in table 1, add the sample volume and the deio...

  • Page 555: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical oxygencod_other_other_md3_eng_ody.Fm page 3 of 8 • if the sample does not flow through the chloride removal cartridge (crc), increase the vacuum until flow starts, then reduce the vacuum down to 20 inches of water. Proceed as usual. • the maximum range...

  • Page 556: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical page 4 of 8 oxygencod_other_other_md3_eng_ody.Fm • to avoid cross-contamination between samples, clean forcep tips between samples by wiping with a clean towel or rinsing with deionized water. • if the sample does not contain suspended solids, it is no...

  • Page 557: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical oxygencod_other_other_md3_eng_ody.Fm page 5 of 8 . 5. Place the vials in the cod reactor at 150 °c. Digest for one hour. 6. Place the vials in a cooling rack for two minutes. If the solution develops a colorless upper layer and a purple lower layer, in...

  • Page 558: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical page 6 of 8 oxygencod_other_other_md3_eng_ody.Fm interferences inorganic materials may also be oxidized by trivalent manganese and constitute a positive interference when present in significant amounts. Chloride is the most common interference and is r...

  • Page 559: Oxygen Demand, Chemical

    Oxygen demand, chemical oxygen demand, chemical oxygencod_other_other_md3_eng_ody.Fm page 7 of 8 3. Touch on . Touch adjust to accept the displayed concentration. If an alternate concentration is used, touch the number in the box to enter the actual concentration, then touch ok . Touch adjust . See ...

  • Page 560: Oxygen Demand, Chemical

    Oxygen demand, chemical © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 8 of 8 oxygencod_other_other_md3_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224...

  • Page 561: Dr/2400

    Dr/2400 oxygen demand, chemical oxygencod_other_other_mn3_eng_ody.Fm page 1 of 2 • for information about sampling, storage, accuracy checks, interferences, method summary, reagents and apparatus, see manganese iii reactor digestion method (with optional chloride removal). • if the sample contains ch...

  • Page 562: Oxygen Demand, Chemical

    Oxygen demand, chemical © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 2 of 2 oxygencod_other_other_mn3_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224...

  • Page 563: Dr/2400

    Dr/2400 oxygen, dissolved oxygendis_accuvac_high_hrd_eng_ody.Fm page 1 of 4 • analyze samples on-site. Do not store for later analysis. Oxygen, dissolved method 8166 hrdo method accuvac ® ampuls hr (0.3 to 15.0 mg/l o 2 ) scope and application: for water and wastewater 1. Touch hach programs . Selec...

  • Page 564: Oxygen, Dissolved

    Oxygen, dissolved oxygen, dissolved page 2 of 4 oxygendis_accuvac_high_hrd_eng_ody.Fm interferences sample collection, preservation, and storage the main consideration in sampling with the high range dissolved oxygen ampul is to prevent the sample from becoming contaminated with atmospheric oxygen b...

  • Page 565: Oxygen, Dissolved

    Oxygen, dissolved oxygen, dissolved oxygendis_accuvac_high_hrd_eng_ody.Fm page 3 of 4 accuracy check the results of this procedure may be compared with the results of a titrimetric procedure (request lit. Code 8042) or sens ion ™6 dissolved oxygen meter (cat. No. 51850-01). Method performance precis...

  • Page 566

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 567: Dr/2400

    Dr/2400 oxygen, dissolved oxygendis_accuvac_low_inc_eng_ody.Fm page 1 of 2 • the ampules will contain a small piece of wire to maintain reagent quality. The solution will be yellow. Oxygen, dissolved method 8316 indigo carmine method accuvac ® ampuls lr (6 to 800 µg/l o 2 ) scope and application: fo...

  • Page 568: Oxygen, Dissolved

    Oxygen, dissolved © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 2 of 2 oxygendis_accuvac_low_inc_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 conta...

  • Page 569: Dr/2400

    Dr/2400 oxygen, dissolved oxygendis_accuvac_uhigh_uhr_eng_ody.Fm page 1 of 4 • analyze samples on-site. Do not store for later analysis. Oxygen, dissolved method 8333 ultra high range method accuvac ® ampul uhr (1.0 to 40.0 mg/l o 2 ) scope and application: for aquaculture 1. Touch hach programs . S...

  • Page 570: Oxygen, Dissolved

    Oxygen, dissolved oxygen, dissolved page 2 of 4 oxygendis_accuvac_uhigh_uhr_eng_ody.Fm interferences sample collection, preservation, and storage the main consideration in sampling with the high range dissolved oxygen accuvac ampul is to prevent the sample from becoming contaminated with atmospheric...

  • Page 571: Oxygen, Dissolved

    Oxygen, dissolved oxygen, dissolved oxygendis_accuvac_uhigh_uhr_eng_ody.Fm page 3 of 4 accuracy check the results of this procedure may be compared with the results of a titrimetric procedure (request lit. Code 8042) or sens ion ™6 dissolved oxygen meter (cat. No. 51850-01). Method performance preci...

  • Page 572

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 573: Dr/2400

    Dr/2400 oxygen scavengers oxygensca_pp_other_ird_eng_ody.Fm page 1 of 4 • temperature and time affect results. Be sure to control these factors as described below. • analyze samples immediately. Do not preserve for later analysis. • the sample temperature should be 25 ±3 °c (77 ±5 °f). • soak glassw...

  • Page 574: Oxygen Scavengers

    Oxygen scavengers oxygen scavengers page 2 of 4 oxygensca_pp_other_ird_eng_ody.Fm 5. Add 0.5 ml of deha reagent 2 solution to each sample cell. Mix. Place both sample cells in the dark. A purple color will develop if an oxygen scavenger is present. 6. Touch the timer icon. Touch ok . A ten-minute re...

  • Page 575: Oxygen Scavengers

    Oxygen scavengers oxygen scavengers oxygensca_pp_other_ird_eng_ody.Fm page 3 of 4 interferences substances which reduce ferric iron will interfere. Substances which complex iron strongly may also interfere. Sample collection, preservation, and storage collect samples in clean, dry, plastic or glass ...

  • Page 576: Oxygen Scavengers

    Oxygen scavengers © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 oxygensca_pp_other_ird_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact ...

  • Page 577: Dr/2400

    Dr/2400 ozone ozone_accuvac_other_ind_eng_ody.Fm page 1 of 4 • analyze sample immediately. Do not preserve for later analysis. • use tap water or deionized water for the blank (ozone-free water). • the sequence of measuring the blank and the sample is reversed in this procedure. Ozone method 8311 in...

  • Page 578: Ozone

    Ozone ozone page 2 of 4 ozone_accuvac_other_ind_eng_ody.Fm sample collection, storage, and preservation the chief consideration when collecting a sample is to prevent the escape of ozone from the sample. The sample should be collected gently and analyzed immediately. Warming the sample, or disturbin...

  • Page 579: Ozone

    Ozone ozone ozone_accuvac_other_ind_eng_ody.Fm page 3 of 4 method performance precision standard: 0.15 mg/l for program number 454 0.45 mg/l for program number 455 1.00 mg/l for program number 456 see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not f...

  • Page 580

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 581: Dr/2400

    Dr/2400 pcb (polychlorinated biphenyls) pcb_none_other_imm_eng_ody.Fm page 1 of 10 this method analyzes for pcb that has been extracted from soil samples. Sample extracts, calibrators, and reagents are added to cuvettes coated with pcb-specific antibodies. The color that develops is then measured an...

  • Page 582

    Pcb (polychlorinated biphenyls) pcb (polychlorinated biphenyls) page 2 of 10 pcb_none_other_imm_eng_ody.Fm 1. Weigh out 5 g of soil in the plastic weighing boat. 2. Carefully pour the soil into an extraction vial. 3. Use the 5-gram scoop to add one scoop of sodium sulfate to the extraction vial. 4. ...

  • Page 583

    Pcb (polychlorinated biphenyls) pcb (polychlorinated biphenyls) pcb_none_other_imm_eng_ody.Fm page 3 of 10 1. Touch single wavelength then touch the λ button. Type in 450 nm and touch ok. Note: use the 1-cm square cell holder for this procedure. 2. Label an antibody cuvette for each calibrator and e...

  • Page 584

    Pcb (polychlorinated biphenyls) pcb (polychlorinated biphenyls) page 4 of 10 pcb_none_other_imm_eng_ody.Fm 9. After 5 minutes, mix the contents of the rack a second time for a period of 30 seconds using the same technique. 10. At the end of the 10-minute period, discard the contents of all the cuvet...

  • Page 585

    Pcb (polychlorinated biphenyls) pcb (polychlorinated biphenyls) pcb_none_other_imm_eng_ody.Fm page 5 of 10 color development note: timing is critical; follow instructions carefully 12. With the cuvettes still held snugly in the rack, pipet 0.5 ml of color developing solution into each antibody cuvet...

  • Page 586: Zero

    Pcb (polychlorinated biphenyls) pcb (polychlorinated biphenyls) page 6 of 10 pcb_none_other_imm_eng_ody.Fm using the wiretrol pipet the wiretrol pipet can accurately measure small quantities of liquids. It consists of two parts: a teflon ® -tipped plunger and a calibrated capillary tube. Use figure ...

  • Page 587

    Pcb (polychlorinated biphenyls) pcb (polychlorinated biphenyls) pcb_none_other_imm_eng_ody.Fm page 7 of 10 figure 1 wiretrol pipet using the 1-cm microcuvette rack this rack (see figure 2) has been designed specifically to aid in achieving precise and accurate results when using the immunoassay tech...

  • Page 588

    Pcb (polychlorinated biphenyls) pcb (polychlorinated biphenyls) page 8 of 10 pcb_none_other_imm_eng_ody.Fm figure 2 the 1-cm microcuvette rack loading the rack — the cuvette rack is designed so that it may be inverted with the cuvettes in place. Identify each cuvette with a sample or calibrator numb...

  • Page 589

    Pcb (polychlorinated biphenyls) pcb (polychlorinated biphenyls) pcb_none_other_imm_eng_ody.Fm page 9 of 10 interpretation interpretation for a soil sample: sample #1 — sample reading is less than the readings for both calibrators. Therefore the sample concentration of pcb is greater than both 1 ppm ...

  • Page 590

    Pcb (polychlorinated biphenyls) © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 10 of 10 pcb_none_other_imm_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4...

  • Page 591: Dr/2400

    Dr/2400 phenols phenols_none_other_4am_eng_ody.Fm page 1 of 6 • analyze samples within four hours to avoid oxidation. • spilled reagent affects test results and is hazardous to skin and other materials. • use chloroform only with proper ventilation. • wipe the outside of sample cells before each ins...

  • Page 592: Phenols

    Phenols phenols page 2 of 6 phenols_none_other_4am_eng_ody.Fm 5. Pour the measured sample into another 500-ml separatory funnel (this is the prepared sample). 6. Add 5 ml of hardness buffer to each separatory funnel. Stopper and shake to mix. 7. Add the contents of one phenol reagent powder pillow t...

  • Page 593: Phenols

    Phenols phenols phenols_none_other_4am_eng_ody.Fm page 3 of 6 interferences sample collection, storage and preservation most reliable results are obtained when samples are analyzed within four hours after collection. Use the following storage instructions only if prompt analysis is not possible. Col...

  • Page 594: Phenols

    Phenols phenols page 4 of 6 phenols_none_other_4am_eng_ody.Fm accuracy check standard solution method for greater accuracy, analyze standard solutions when new lots of reagent are first used. 1. Weigh out 1.00 g of phenol, acs. Transfer to a 1000-ml volumetric flask. Dilute to the mark with freshly ...

  • Page 595: Phenols

    Phenols phenols phenols_none_other_4am_eng_ody.Fm page 5 of 6 distillation this procedure is in the hach distillation apparatus manual in step-by-step illustrated format. 1. Set up the hach distillation apparatus by assembling the general purpose apparatus as shown in the distillation apparatus manu...

  • Page 596: Phenols

    Phenols © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 phenols_none_other_4am_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach o...

  • Page 597: Dr/2400

    Dr/2400 phosphonates phosphonates_pp_other_puv_eng_ody.Fm page 1 of 6 • clean glassware with 1:1 hydrochloric acid solution (cat. No. 884-49), followed by a distilled water rinse. Do not clean glassware with commercial detergent. • wear uv safety goggles while the uv lamp is on. • do not handle the ...

  • Page 598: Phosphonates

    Phosphonates phosphonates page 2 of 6 phosphonates_pp_other_puv_eng_ody.Fm 5. Add the contents of one potassium persulfate for phosphonate powder pillow to the bottle containing 25 ml of sample. Swirl to mix. 6. Insert the ultraviolet (uv) lamp into the sample bottle. 7. Turn on the uv lamp. Touch t...

  • Page 599: Phosphonates

    Phosphonates phosphonates phosphonates_pp_other_puv_eng_ody.Fm page 3 of 6 to express results in terms of active phosphonate, multiply the final value in step 15 by the appropriate conversion factor in table 2. 13. Touch zero . The display will show: 0.00 mg/l po 4 3– 14. Wipe the prepared sample an...

  • Page 600: Phosphonates

    Phosphonates phosphonates page 4 of 6 phosphonates_pp_other_puv_eng_ody.Fm interferences the interference levels will decrease as the sample size increases. For example, copper does not interfere at or below 100 mg/l for a 5.00 ml sample. If the sample volume is increased to 10 ml, copper will begin...

  • Page 601: Phosphonates

    Phosphonates phosphonates phosphonates_pp_other_puv_eng_ody.Fm page 5 of 6 accuracy check ideally, a solution containing the phosphonate product being used should be prepared. This will check the uv conversion of phosphonate to orthophosphate. Alternatively, a phosphate standard can be used to check...

  • Page 602: Phosphonates

    Phosphonates © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 phosphonates_pp_other_puv_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact th...

  • Page 603: Dr/2400

    Dr/2400 phosphorus, acid hydrolyzable phosphorusacidhyd_tnt_other_phy_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final re...

  • Page 604: Zero

    Phosphorus, acid hydrolyzable phosphorus, acid hydrolyzable page 2 of 6 phosphorusacidhyd_tnt_other_phy_eng_ody.Fm 5. Touch the timer icon. Touch ok . A 30-minute heating period will begin. 6. After the timer beeps, carefully remove the vial from the reactor. Place it in a test tube rack and cool to...

  • Page 605

    Phosphorus, acid hydrolyzable phosphorus, acid hydrolyzable phosphorusacidhyd_tnt_other_phy_eng_ody.Fm page 3 of 6 interferences sample collection, storage, and preservation collect samples in plastic or glass bottles that have been acid washed with 1:1 hydrochloric acid solution (cat. No. 884-49) a...

  • Page 606

    Phosphorus, acid hydrolyzable phosphorus, acid hydrolyzable page 4 of 6 phosphorusacidhyd_tnt_other_phy_eng_ody.Fm 5. Prepare three sample spikes. Fill three mixing cylinders (cat. No. 1896-40) with 25 ml of sample. Use the tensette pipet to add 0.1 ml, 0.2 ml, and 0.3 ml of standard, respectively, ...

  • Page 607

    Phosphorus, acid hydrolyzable phosphorus, acid hydrolyzable phosphorusacidhyd_tnt_other_phy_eng_ody.Fm page 5 of 6 required reagents quantity required description per test unit cat. No total and acid hydrolyzable phosphorus reagent set................................................ 50 tests ..........

  • Page 608

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 609: Dr/2400

    Dr/2400 phosphorus, acid hydrolyzable digestion phosphorusacidhydro_other_ad_eng_ody.Fm page 1 of 4 • rinse all glassware with 1:1 hydrochloric acid. Rinse again with deionized water. • the results of the reactive phosphorus test after the digestion will include the orthophosphate and the acid-hydro...

  • Page 610

    Phosphorus, acid hydrolyzable digestion phosphorus, acid hydrolyzable digestion page 2 of 4 phosphorusacidhydro_other_ad_eng_ody.Fm interferences sample collection, storage, and preservation analyze the samples immediately for the most reliable results. If prompt analysis is not possible, samples ma...

  • Page 611

    Phosphorus, acid hydrolyzable digestion phosphorus, acid hydrolyzable digestion phosphorusacidhydro_other_ad_eng_ody.Fm page 3 of 4 summary of method phosphates present in condensed inorganic forms (meta-, pyro- or other polyphosphates) must be converted to reactive orthophosphate before analysis. P...

  • Page 612

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 613: Dr/2400

    Dr/2400 phosphorus, reactive phosphorusreac_pt_low_asc_eng_ody.Fm page 1 of 6 • clean the pour-thru cell and all labware as specified in treating analysis labware on page 3. • protect the pour-thru cell from contamination when not in use by inverting a small beaker over the top of the glass funnel. ...

  • Page 614: Phosphorus, Reactive

    Phosphorus, reactive phosphorus, reactive page 2 of 6 phosphorusreac_pt_low_asc_eng_ody.Fm 5. Fill this rinsed cylinder to the 25-ml mark with sample. 6. Pour the contents of the 25-ml cylinder into one of the flasks. 7. Measure a second 25-ml portion of sample into the graduated cylinder and pour t...

  • Page 615: Phosphorus, Reactive

    Phosphorus, reactive phosphorus, reactive phosphorusreac_pt_low_asc_eng_ody.Fm page 3 of 6 interferences treating analysis labware all labware used in this test must be thoroughly cleaned to remove all traces of phosphate. Clean containers with a non-phosphate detergent followed by a rinse with deio...

  • Page 616: Phosphorus, Reactive

    Phosphorus, reactive phosphorus, reactive page 4 of 6 phosphorusreac_pt_low_asc_eng_ody.Fm sampling and storage collect samples in clean plastic or glass bottles that have been cleaned with 1:1 hydrochloric acid solution (cat. No. 884-49) and rinsed with deionized water. Do not use detergents that c...

  • Page 617: Phosphorus, Reactive

    Phosphorus, reactive phosphorus, reactive phosphorusreac_pt_low_asc_eng_ody.Fm page 5 of 6 method performance precision standard: 1000 µg/l po 4 3– see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see s...

  • Page 618

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 619: Dr/2400

    Dr/2400 phosphorus, reactive (orthophosphate) phosphorusreac_none_other_ami_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the fi...

  • Page 620: Zero

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) page 2 of 6 phosphorusreac_none_other_ami_eng_ody.Fm 5. Touch the timer icon. Touch ok . A 10-minute reaction period will begin. Continue with step 6 while the timer is running. 6. Pour 25 ml of sample into a round sample ce...

  • Page 621

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) phosphorusreac_none_other_ami_eng_ody.Fm page 3 of 6 interferences sample collection, storage, and preservation collect samples in clean plastic or glass bottles that have been cleaned with 1:1 hydrochloric acid solution (ca...

  • Page 622

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) page 4 of 6 phosphorusreac_none_other_ami_eng_ody.Fm 3. Touch ok to accept the default values for standard concentration, sample volume, and spike volumes. Touch edit to change these values. After values are accepted, the un...

  • Page 623

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) phosphorusreac_none_other_ami_eng_ody.Fm page 5 of 6 summary of method in a highly acidic solution, ammonium molybdate reacts with orthophosphate to form molybdophosphoric acid. This complex is then reduced by the amino acid...

  • Page 624

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 625: Dr/2400

    Dr/2400 phosphorus, reactive (orthophosphate) phosphorusreac_accuvac_other_mol_eng_ody.Fm page 1 of 6 ì • for best results, sample temperature should be 20–25 °c (68–77 °f). • after adding reagent, a yellow color will form if phosphate is present. The blank will be slightly yellow because of the rea...

  • Page 626: Zero

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) page 2 of 6 phosphorusreac_accuvac_other_mol_eng_ody.Fm 5. Touch the timer icon. Touch ok . A three-minute reaction period will begin. 6. When the timer beeps, place the blank into the cell holder. 7. Touch zero . The displa...

  • Page 627: Zero

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) phosphorusreac_accuvac_other_mol_eng_ody.Fm page 3 of 6 interferences 5. When the timer beeps, place the blank into the cell holder. 6. Touch zero . The display will show: 0.0 mg/l po 4 3– 7. Place the prepared sample into t...

  • Page 628

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) page 4 of 6 phosphorusreac_accuvac_other_mol_eng_ody.Fm the following do not interfere in concentrations up to 1000 mg/l: sample collection, storage and preservation collect samples in clean plastic or glass bottles that hav...

  • Page 629

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) phosphorusreac_accuvac_other_mol_eng_ody.Fm page 5 of 6 view: fit , then select ideal line and touch ok to view the relationship between the sample spikes and the “ideal line” of 100% recovery. See section 3.2.2 standard add...

  • Page 630

    Phosphorus, reactive (orthophosphate) © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 phosphorusreac_accuvac_other_mol_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and orderi...

  • Page 631: Dr/2400

    Dr/2400 phosphorus, reactive (orthophosphate) phosphorusreac_tnt_high_mol_eng_ody.Fm page 1 of 4 • reagent blanks for each lot of reagents may be used more than once. At room temperature, the reagent blank is stable for up to three weeks. • the seven-minute reaction time in step 4 is for samples at ...

  • Page 632: Zero

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) page 2 of 4 phosphorusreac_tnt_high_mol_eng_ody.Fm interferences 5. Install the 16-mm adapter. Note: see section 2.6 in the instrument manual for installation details. When the timer beeps, place the sample vial into the ada...

  • Page 633

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) phosphorusreac_tnt_high_mol_eng_ody.Fm page 3 of 4 sampling and storage collect samples in plastic or glass bottles that have been cleaned with 1:1 hydrochloric acid solution (cat. No. 884-49) and rinsed with deionized water...

  • Page 634

    Phosphorus, reactive (orthophosphate) © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 phosphorusreac_tnt_high_mol_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: c...

  • Page 635: Dr/2400

    Dr/2400 phosphorus, reactive phosphorusreac_pt_high_mol_eng_ody.Fm page 1 of 6 • clean the pour-thru cell and all labware as specified in treating analysis labware on page 4. • protect the pour-thru cell from contamination when not in use by inverting a small beaker over the top of the glass funnel....

  • Page 636: Phosphorus, Reactive

    Phosphorus, reactive phosphorus, reactive page 2 of 6 phosphorusreac_pt_high_mol_eng_ody.Fm 5. Rinse another clean plastic 125-ml erlenmeyer flask and a 25-ml graduated cylinder with deionized water. 6. Measure 25 ml of sample in the graduated cylinder. Pour the water into the flask (this is the sam...

  • Page 637: Phosphorus, Reactive

    Phosphorus, reactive phosphorus, reactive phosphorusreac_pt_high_mol_eng_ody.Fm page 3 of 6 interferences the following do not interfere in concentrations up to 1000 mg/l: interfering substance interference levels and treatments arsenate negative interference. Positive interference if sample is heat...

  • Page 638: Phosphorus, Reactive

    Phosphorus, reactive phosphorus, reactive page 4 of 6 phosphorusreac_pt_high_mol_eng_ody.Fm sampling and storage collect samples in clean plastic or glass bottles that have been cleaned with 1:1 hydrochloric acid solution (cat. No. 884-49) and rinsed with deionized water. Do not use detergents that ...

  • Page 639: Phosphorus, Reactive

    Phosphorus, reactive phosphorus, reactive phosphorusreac_pt_high_mol_eng_ody.Fm page 5 of 6 standard solution method 1. Use a 10.0-mg/l phosphate standard in place of the sample. Results should be between 9.0 and 11.0 mg/l phosphate. 2. To adjust the calibration curve using the reading obtained with...

  • Page 640: Phosphorus, Reactive

    Phosphorus, reactive © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 phosphorusreac_pt_high_mol_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 c...

  • Page 641: Dr/2400

    Dr/2400 phosphorus, reactive (orthophosphate) phosphorusreac_avpp_other_pv3_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the fi...

  • Page 642: Zero

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) page 2 of 6 phosphorusreac_avpp_other_pv3_eng_ody.Fm 5. Fill another sample cell with 10 ml of sample (this is the blank). 6. When the timer beeps, wipe the blank and place it into the cell holder. 7. Touch zero . The displa...

  • Page 643: Zero

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) phosphorusreac_avpp_other_pv3_eng_ody.Fm page 3 of 6 interferences sample collection, storage, and preservation collect sample in plastic or glass bottles that have been cleaned with 1:1 hydrochloric acid solution (cat. No. ...

  • Page 644

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) page 4 of 6 phosphorusreac_avpp_other_pv3_eng_ody.Fm analyze samples immediately for best results. If prompt analysis is not possible, preserve samples by filtering immediately and storing at 4 °c (39 °f) for up to 48 hours....

  • Page 645

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) phosphorusreac_avpp_other_pv3_eng_ody.Fm page 5 of 6 method performance precision standard: 1.00 mg/l po 4 3– see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within ...

  • Page 646

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 647: Dr/2400

    Dr/2400 phosphorus, reactive (orthophosphate) phosphorusreac_tnt_other_pv3_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the fin...

  • Page 648: Zero

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) page 2 of 6 phosphorusreac_tnt_other_pv3_eng_ody.Fm interferences 5. Touch zero . The display will show: 0.00 mg/l po 4 3– 6. Using a funnel, add the contents of one phosver 3 phosphate powder pillow to the vial. 7. Cap the ...

  • Page 649

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) phosphorusreac_tnt_other_pv3_eng_ody.Fm page 3 of 6 sample collection, storage, and preservation collect samples in plastic or glass bottles that have been acid cleaned with 1:1 hydrochloric acid solution (cat. No. 884-49) a...

  • Page 650

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) page 4 of 6 phosphorusreac_tnt_other_pv3_eng_ody.Fm 8. After completing the sequence, touch graph to view the best-fit line through the standard additions data points, accounting for matrix interferences. Touch view: fit , t...

  • Page 651

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) phosphorusreac_tnt_other_pv3_eng_ody.Fm page 5 of 6 required reagents quantity required description per test unit cat. No. Reactive phosphorus test ’n tube™ reagent set (50 tests)................................................

  • Page 652

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 653: Dr/2400

    Dr/2400 phosphorus, reactive (orthophosphate) phosphorusreac_unicell_asc_eng_ody.Fm page 1 of 4 • adjust the ph of preserved samples between ph 2–10 before testing. • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel, followed by a dry one. • use the standard ...

  • Page 654: Zero

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) page 2 of 4 phosphorusreac_unicell_asc_eng_ody.Fm interferences the ions listed in the following table have been individually tested up to the given concentrations. Cumulative effects and the influence of other ions have not...

  • Page 655

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) phosphorusreac_unicell_asc_eng_ody.Fm page 3 of 4 sample collection, storage, and preservation analyze samples within 3 hours after collection for best results. Store in a cool dry place in plastic or glass bottles that have...

  • Page 656

    Phosphorus, reactive (orthophosphate) © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 phosphorusreac_unicell_asc_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: ca...

  • Page 657: Dr/2400

    Dr/2400 phosphorus, reactive (orthophosphate) phosphorusreac_unicell_other_asc_eng_ody.Fm page 1 of 4 • adjust the ph of preserved samples to between ph 2–10 before testing. • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel, followed by a dry one. • use the ...

  • Page 658: Zero

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) page 2 of 4 phosphorusreac_unicell_other_asc_eng_ody.Fm interferences the ions listed in the following table have been individually tested up to the given concentrations. Cumulative effects and the influence of other ions ha...

  • Page 659

    Phosphorus, reactive (orthophosphate) phosphorus, reactive (orthophosphate) phosphorusreac_unicell_other_asc_eng_ody.Fm page 3 of 4 sample collection, storage, and preservation analyze samples within 3 hours after collection for best results. Store in a cool dry place in plastic or glass bottles tha...

  • Page 660

    Phosphorus, reactive (orthophosphate) © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 phosphorusreac_unicell_other_asc_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and orderi...

  • Page 661: Dr/2400

    Dr/2400 phosphorus, total, digestion phosphorustot_pp_other_apd_eng_ody.Fm page 1 of 4 • rinse all glassware with 1:1 hydrochloric acid solution (cat. No. 884-49). Rinse again with deionized water. • the results of the reactive phosphorus test after the digestion will include the organic phosphate p...

  • Page 662: Phosphorus, Total, Digestion

    Phosphorus, total, digestion phosphorus, total, digestion page 2 of 4 phosphorustot_pp_other_apd_eng_ody.Fm interferences sample collection, storage, and preservation analyze the samples immediately for the most reliable results. If prompt analysis is not possible, samples may be preserved up to 28 ...

  • Page 663: Phosphorus, Total, Digestion

    Phosphorus, total, digestion phosphorus, total, digestion phosphorustot_pp_other_apd_eng_ody.Fm page 3 of 4 summary of method phosphates present in organic and condensed inorganic forms (meta-, pyro-, or other polyphosphates) must be converted to reactive orthophosphate before analysis. Pretreatment...

  • Page 664

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 665: Dr/2400

    Dr/2400 phosphorus, total phosphorustot_tnt_other_pap_eng_ody.Fm page 1 of 6 • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtract the reagent blank value from the final results or perform...

  • Page 666: Phosphorus, Total

    Phosphorus, total phosphorus, total page 2 of 6 phosphorustot_tnt_other_pap_eng_ody.Fm 5. Cap tightly and shake to dissolve. 6. Place the vial into the cod reactor. 7. Touch the timer icon. Touch ok . A 30-minute heating period will begin. 8. When the timer beeps, carefully remove the hot vial from ...

  • Page 667: Phosphorus, Total

    Phosphorus, total phosphorus, total phosphorustot_tnt_other_pap_eng_ody.Fm page 3 of 6 13. Use a funnel to add the contents of one phosver 3 powder pillow to the vial. 14. Cap tightly and shake to mix for 10–15 seconds. The powder will not dissolve completely. 15. Touch the timer icon. Touch ok . A ...

  • Page 668: Phosphorus, Total

    Phosphorus, total phosphorus, total page 4 of 6 phosphorustot_tnt_other_pap_eng_ody.Fm interferences sample collection, storage, and preservation collect samples in plastic or glass bottles that have been acid washed with 1:1 hydrochloric acid solution (cat. No. 884-49) and rinsed with deionized wat...

  • Page 669: Phosphorus, Total

    Phosphorus, total phosphorus, total phosphorustot_tnt_other_pap_eng_ody.Fm page 5 of 6 6. Prepare three sample spikes. Fill three mixing cylinders (cat. No. 1896-40) with 25 ml of sample. Use the tensette pipet to add 0.1 ml, 0.2 ml, and 0.3 ml of standard, respectively, to each sample and mix thoro...

  • Page 670: Phosphorus, Total

    Phosphorus, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 phosphorustot_tnt_other_pap_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 con...

  • Page 671: Dr/2400

    Dr/2400 phosphorus, total phosphorustot_tnt_high_mol_eng_ody.Fm page 1 of 6 • place a safety shield in front of the cod reactor to prevent injury if splattering occurs. • reagent blanks can be used more than once, but should not be used more than one day. • the final samples will contain molybdenum....

  • Page 672: Phosphorus, Total

    Phosphorus, total phosphorus, total page 2 of 6 phosphorustot_tnt_high_mol_eng_ody.Fm 5. Use a funnel to add the contents of one potassium persulfate powder pillow for phosphonate to each vial. Cap tightly and shake to dissolve. 6. Place the vials in the cod reactor. 7. Touch the timer icon. Touch o...

  • Page 673: Phosphorus, Total

    Phosphorus, total phosphorus, total phosphorustot_tnt_high_mol_eng_ody.Fm page 3 of 6 interferences large amounts of sample turbidity may cause inconsistent results in the test because the acid present in the reagents may dissolve some of the suspended particles and because of variable desorption of...

  • Page 674: Phosphorus, Total

    Phosphorus, total phosphorus, total page 4 of 6 phosphorustot_tnt_high_mol_eng_ody.Fm sampling and storage collect samples in plastic or glass bottles that have been acid washed with 1:1 hydrochloric acid solution (cat. No. 884-49) and rinsed with deionized water. Do not use commercial detergents co...

  • Page 675: Phosphorus, Total

    Phosphorus, total phosphorus, total phosphorustot_tnt_high_mol_eng_ody.Fm page 5 of 6 3. Touch on . Touch adjust to accept the displayed concentration (the value depends on the selected chemical form). If an alternate concentration is used, touch the number in the box to enter the actual concentrati...

  • Page 676: Phosphorus, Total

    Phosphorus, total © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 phosphorustot_tnt_high_mol_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 cont...

  • Page 677: Dr/2400

    Dr/2400 phosphorus, total phosphorustot_unicell_apd_eng_ody.Fm page 1 of 6 0 • adjust the ph of preserved samples to between ph 2–10 before testing. • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel followed by a dry one. • use the standard solution or stand...

  • Page 678: Phosphorus, Total

    Phosphorus, total phosphorus, total page 2 of 6 phosphorustot_unicell_apd_eng_ody.Fm 5. Place the vial into the reactor block. Heat at 100 °c for 60 minutes. 6. After the 60-minute heating period, carefully remove the hot vial from the reactor. Place it in a test tube rack and cool to room temperatu...

  • Page 679: Phosphorus, Total

    Phosphorus, total phosphorus, total phosphorustot_unicell_apd_eng_ody.Fm page 3 of 6 interferences the ions listed in the following table have been individually tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. Sample collection, stora...

  • Page 680: Phosphorus, Total

    Phosphorus, total phosphorus, total page 4 of 6 phosphorustot_unicell_apd_eng_ody.Fm for wastewater samples — if prompt analysis is impossible, preserve samples * by adjusting the ph to 2 or less with sulfuric acid, 5.25 n (cat. No. 2449-00) and storing the sample at 4 °c for up to 28 days. The ph c...

  • Page 681: Phosphorus, Total

    Phosphorus, total phosphorus, total phosphorustot_unicell_apd_eng_ody.Fm page 5 of 6 method performance precision standard: 7.5 mg/l po 4 3– see section 3.4.3 precision on page 44 for more information, or if the standard concentration did not fall within the specified range. Sensitivity see section ...

  • Page 682

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 683: Dr/2400

    Dr/2400 phosphorus, total phosphorustot_unicell_other_apd_eng_ody.Fm page 1 of 6 • adjust the ph of preserved samples to between ph 2–10 before testing. • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel, followed by a dry one. • use the standard solution or ...

  • Page 684: Phosphorus, Total

    Phosphorus, total phosphorus, total page 2 of 6 phosphorustot_unicell_other_apd_eng_ody.Fm 5. Place the vial into the reactor block. Heat at 100 °c for 60 minutes. 6. After the 60-minute heating period, carefully remove the hot vial from the reactor. Place it in a tube rack and cool to room temperat...

  • Page 685: Phosphorus, Total

    Phosphorus, total phosphorus, total phosphorustot_unicell_other_apd_eng_ody.Fm page 3 of 6 interferences the ions listed in the following table have been individually tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. Sample collection,...

  • Page 686: Phosphorus, Total

    Phosphorus, total phosphorus, total page 4 of 6 phosphorustot_unicell_other_apd_eng_ody.Fm 6. Prepare three sample spikes. Fill three mixing cylinders with 25 ml of sample. Use a pipet to add 0.1 ml, 0.2 ml, and 0.3 ml of standard, respectively, to each cylinder. Mix well. 7. Analyze each sample spi...

  • Page 687: Phosphorus, Total

    Phosphorus, total phosphorus, total phosphorustot_unicell_other_apd_eng_ody.Fm page 5 of 6 required reagents description unit cat. No. Phosphate - po 4 3– , unicell™ hct 122........................................................................... 23/pkg.............. Hct 122 required apparatus ada...

  • Page 688

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 689: Dr/2400

    Dr/2400 potassium potassium_pp_other_tpb_eng_ody.Fm page 1 of 6 • program # 905 has a calibration curve for potassium; however, due to potential variation between lots of potassium 3 reagent, perform a new calibration for each lot of reagent to obtain best accuracy. Prepare and store the calibration...

  • Page 690: Potassium

    Potassium potassium page 2 of 6 potassium_pp_other_tpb_eng_ody.Fm interferences the substances listed below have been tested and will not interfere at or below the levels stated. If these substances are present at higher levels, conduct interference studies at the higher levels to determine if the s...

  • Page 691: Potassium

    Potassium potassium potassium_pp_other_tpb_eng_ody.Fm page 3 of 6 sample collection, preservation, and storage collect samples in acid-washed plastic bottles. Adjust the ph to 2 or less with nitric acid (about 2 ml per liter) (cat. No. 2540-49). Preserved samples may be stored at least six months at...

  • Page 692: Potassium

    Potassium potassium page 4 of 6 potassium_pp_other_tpb_eng_ody.Fm user programming 1. Touch user programs on the main menu. 2. If you have not performed a potassium calibration before, touch program options and new program . 3. Assign a program number to the potassium user program. Touch ok . 4. Sel...

  • Page 693: Potassium

    Potassium potassium potassium_pp_other_tpb_eng_ody.Fm page 5 of 6 summary of method potassium in the sample reacts with sodium tetraphenylborate to form potassium tetraphenylborate, an insoluble white solid. The amount of turbidity produced is proportional to the potassium concentration. Test result...

  • Page 694

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 695: Dr/2400

    Dr/2400 quaternary ammonium compounds quaternaryammcom_pp_other_dbc_eng_ody.Fm page 1 of 4 quaternary ammonium compounds method 8337 direct binary complex method powder pillows (0.2 to 5.0 mg/l as ctab) scope and application: for cooling tower water and pool/spa water 1. Touch hach programs . Select...

  • Page 696

    Quaternary ammonium compounds quaternary ammonium compounds page 2 of 4 quaternaryammcom_pp_other_dbc_eng_ody.Fm interferences interference studies were conducted by preparing a ctab standard solution of approximately 3 mg/l as well as a solution of the potential interference. The constituent was sa...

  • Page 697

    Quaternary ammonium compounds quaternary ammonium compounds quaternaryammcom_pp_other_dbc_eng_ody.Fm page 3 of 4 sample collection, storage, and preservation collect samples in glass bottles that have been rinsed several times with sample before final sample filling. Do not use plastic containers as...

  • Page 698

    Quaternary ammonium compounds © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 quaternaryammcom_pp_other_dbc_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 80...

  • Page 699: Dr/2400

    Dr/2400 selenium selenium_none_other_dab_eng_ody.Fm page 1 of 8 • distillation is required for determining total selenium. See the distillation on page 4 at the end of the procedure. Use the distillate in step 2. • acetone (cat. No. 14429-49) is a suitable solvent for removing toluene from glassware...

  • Page 700: Selenium

    Selenium selenium page 2 of 8 selenium_none_other_dab_eng_ody.Fm 5. If you have not distilled the sample, add 5.0 ml of buffer solution, sulfate type, ph 2.0 to each flask. Swirl to mix. If the sample has been distilled, adjust the ph of the distillate to ph 2.7 ±0.2 using 5 n sodium hydroxide stand...

  • Page 701: Selenium

    Selenium selenium selenium_none_other_dab_eng_ody.Fm page 3 of 8 13. Touch the timer icon. Touch ok . A 30-second reaction period will begin. During this time, vigorously shake the funnel that contains the sample. 14. Touch the timer icon. Touch ok . A four-minute reaction period will begin. 15. Whe...

  • Page 702: Selenium

    Selenium selenium page 4 of 8 selenium_none_other_dab_eng_ody.Fm interferences note: there are no positive inorganic interferences with this method. Sample collection, storage, and preservation collect samples in clean glass or plastic containers. Adjust the ph to 2 or less with nitric acid (cat. No...

  • Page 703: Selenium

    Selenium selenium selenium_none_other_dab_eng_ody.Fm page 5 of 8 14. Fill a 250-ml beaker to the 75-ml mark with deionized water. Place it under the drip tube. Elevate the beaker with a laboratory jack so the tube extends below the level of the water. 15. Add 1.0 ml of 30% hydrogen peroxide solution...

  • Page 704: Selenium

    Selenium selenium page 6 of 8 selenium_none_other_dab_eng_ody.Fm view: fit , then select ideal line and touch ok to view the relationship between the sample spikes and the “ideal line” of 100% recovery. See section 3.2.2 standard additions on page 32 for more information. Standard solution method pr...

  • Page 705: Selenium

    Selenium selenium selenium_none_other_dab_eng_ody.Fm page 7 of 8 required reagents quantity required description per test unit cat. No. Selenium reagent set (100 tests) ...................................................................................................................22442-00 include...

  • Page 706

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 707: Dr/2400

    Dr/2400 silica silica_pp_high_slm_eng_ody.Fm page 1 of 4 • sample temperature should be 15–25 °c (59–77 °f). Silica method 8185 silicomolybdate method powder pillows hr (1.0 to 100.0 mg/l) scope and application: for water and seawater 1. Touch hach programs . Select program 656 silica hr . Touch sta...

  • Page 708: Silica

    Silica silica page 2 of 4 silica_pp_high_slm_eng_ody.Fm interferences occasionally a sample contains silica which reacts very slowly with molybdate. The nature of these “molybdate-unreactive” forms is not known. A pretreatment with sodium bicarbonate (cat. No. 776-01), then sulfuric acid (cat. No. 1...

  • Page 709: Silica

    Silica silica silica_pp_high_slm_eng_ody.Fm page 3 of 4 standard methods for the examination of water and wastewater under silica-digestion with sodium bicarbonate. A longer reaction time with the sample and the molybdate and acid reagents (before adding citric acid) may help instead of the bicarbon...

  • Page 710: Silica

    Silica © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 silica_pp_high_slm_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office...

  • Page 711: Dr/2400

    Dr/2400 silica silica_pp_low_htb_eng_ody.Fm page 1 of 4 • the four-minute reaction time in step 4 is for samples at 20 °c; for samples at 10 °c, wait eight minutes; for samples at 30 °c, wait two minutes. • the one-minute reaction time in step 6 is for samples at 20 °c; for samples at 10 °c, wait tw...

  • Page 712: Silica

    Silica silica page 2 of 4 silica_pp_low_htb_eng_ody.Fm 5. When the timer beeps, add the contents of one citric acid reagent powder pillow to each sample cell. Swirl to mix. 6. Touch the timer icon. Touch ok . A one-minute reaction period will begin. The destruction of possible phosphate interference...

  • Page 713: Silica

    Silica silica silica_pp_low_htb_eng_ody.Fm page 3 of 4 interferences sample collection, storage, and preservation collect samples in clean plastic bottles. Analyze samples as soon as possible after collection. If prompt analysis is not possible, store samples for up to 7 days by cooling to 4 °c (39 ...

  • Page 714: Silica

    Silica © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 silica_pp_low_htb_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office ...

  • Page 715: Dr/2400

    Dr/2400 silica silica_pt_ulow_htb_eng_ody.Fm page 1 of 6 • reagent blank values printed on analyzer reagent containers vary because the reagents’ dilutions vary according to instrument. For this method, use the 1234d analyzer reagent blank value for a 3.78 l volume of molybdate 3 reagent (cat. No. 1...

  • Page 716: Silica

    Silica silica page 2 of 6 silica_pt_ulow_htb_eng_ody.Fm 5. Fill two clean 250-ml erlenmeyer flasks to overflowing with sample. 6. Fill a clean 50-ml plastic graduated cylinder with sample from one of the flasks; then discard the contents of the cylinder. Repeat three times. 7. Fill the rinsed cylind...

  • Page 717: Silica

    Silica silica silica_pt_ulow_htb_eng_ody.Fm page 3 of 6 interferences sample collection, storage, and preservation use only plastic containers with tight-fitting closures. Do not use glass containers; they will contaminate the sample with silica. Soak sampling containers with a solution made of one ...

  • Page 718: Silica

    Silica silica page 4 of 6 silica_pt_ulow_htb_eng_ody.Fm reagent preparation amino acid f reagent solution is available in either 100-ml bottles or a package of 20 unit-dose ampules. The bottled reagent is stable for up to one year if the bottle is kept closed when not in use. The ampuled reagent is ...

  • Page 719: Silica

    Silica silica silica_pt_ulow_htb_eng_ody.Fm page 5 of 6 3. Touch ok to accept the default values for standard concentration, sample volume, and spike volumes. Touch edit to change these values. After values are accepted, the unspiked sample reading will appear in the top row. See standard additions ...

  • Page 720: Silica

    Silica © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 silica_pt_ulow_htb_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office...

  • Page 721: Dr/2400

    Dr/2400 silica silica_ptrl_ulow_htb_eng_ody.Fm page 1 of 6 • reagent blank values printed on analyzer reagent containers vary because the reagents’ dilutions vary according to instrument. For this method, use the 1234d analyzer reagent blank value for a 3.78 l volume of molybdate 3 reagent (cat. No....

  • Page 722: Silica

    Silica silica page 2 of 6 silica_ptrl_ulow_htb_eng_ody.Fm 5. Fill two clean 250-ml erlenmeyer flasks to overflowing with sample. 6. Fill a clean 50-ml plastic graduated cylinder with sample from one of the flasks; then discard the contents. Repeat three times. 7. Fill the rinsed cylinder to the 50-m...

  • Page 723: Silica

    Silica silica silica_ptrl_ulow_htb_eng_ody.Fm page 3 of 6 interferences 13. Touch the timer icon. Touch ok . A one-minute reaction period will begin. The destruction of possible phosphate interference occurs during this period. 14. When the timer beeps, pour the contents of one flask into the pour-t...

  • Page 724: Silica

    Silica silica page 4 of 6 silica_ptrl_ulow_htb_eng_ody.Fm sample collection, storage, and preservation use only plastic containers with tight-fitting closures. Do not use glass containers; they will contaminate the sample with silica. Soak sampling containers with a solution made of one part molybda...

  • Page 725: Silica

    Silica silica silica_ptrl_ulow_htb_eng_ody.Fm page 5 of 6 accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. Verify the chemical form. 2. Touch options . Touch standard additions . A summary of the standar...

  • Page 726: Silica

    Silica © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 silica_ptrl_ulow_htb_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach offi...

  • Page 727: Dr/2400

    Dr/2400 silver silver_pp_other_clr_eng_ody.Fm page 1 of 6 • digestion is required in samples with interferences. See digestion on page 4. • for more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample. Subtr...

  • Page 728: Silver

    Silver silver page 2 of 6 silver_pp_other_clr_eng_ody.Fm interferences interference studies were conducted by preparing a known silver solution (about 0.4 mg/l) and the potential interfering ion. The ion was said to interfere when the resulting concentration changed by ±10%. 5. Fill a cell to the 25...

  • Page 729: Silver

    Silver silver silver_pp_other_clr_eng_ody.Fm page 3 of 6 sample collection, storage, and preservation collect samples in acid-cleaned glass or plastic bottles. Using ph paper, adjust the ph to 2 or less with concentrated nitric acid (cat. No. 152-49) (about 2 ml/liter). Store preserved samples at ro...

  • Page 730: Silver

    Silver silver page 4 of 6 silver_pp_other_clr_eng_ody.Fm standard solution method prepare a 0.5 mg/l silver standard solution as follows: 1. Pipet 0.50 ml of silver standard solution, 1000 mg/l, into a 1000-ml volumetric flask using a class a volumetric pipet. Dilute to the mark with deionized water...

  • Page 731: Silver

    Silver silver silver_pp_other_clr_eng_ody.Fm page 5 of 6 3. Add 3 ml of concentrated sulfuric acid to the sample in the volumetric flask. Immediately place the head on the digestion flask. Never use less than 3 ml of acid. 4. Place the digestion flask on the heater. Turn the temperature dial to 440 ...

  • Page 732: Silver

    Silver © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 silver_pp_other_clr_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach offic...

  • Page 733: Dr/2400

    Dr/2400 sulfate sulfate_avpp_other_sv4_eng_ody.Fm page 1 of 6 • you must adjust the standard curve for each new lot of reagent. See standard solutions following these steps. • for best results, perform a new calibration for each lot of reagent. See calibration standard preparation following these st...

  • Page 734: Sulfate

    Sulfate sulfate page 2 of 6 sulfate_avpp_other_sv4_eng_ody.Fm 5. Fill a second sample cell with 10 ml of sample (the blank). 6. When the timer beeps, place the blank into the cell holder. 7. Touch zero . The display will show: 0 mg/l so 4 2– 8. Within five minutes after the timer beeps, place the pr...

  • Page 735: Sulfate

    Sulfate sulfate sulfate_avpp_other_sv4_eng_ody.Fm page 3 of 6 interferences sample collection, storage, and preservation collect samples in clean plastic or glass bottles. Samples may be stored up to 7 days by cooling to 4 °c (39 °f) or lower. Warm to room temperature before analysis. 5. Wipe off an...

  • Page 736: Sulfate

    Sulfate sulfate page 4 of 6 sulfate_avpp_other_sv4_eng_ody.Fm accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Touch options . Touch standard additions . A summary of the standard additions procedure ...

  • Page 737: Sulfate

    Sulfate sulfate sulfate_avpp_other_sv4_eng_ody.Fm page 5 of 6 calibration standard preparation to perform a sulfate calibration using the sulfaver method, use class a glassware to prepare calibration standards containing 10, 20, 30, 40, 50, 60 and 70 mg/l so 4 2– as follows: 1. Into seven different ...

  • Page 738

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 739: Dr/2400

    Dr/2400 sulfate sulfate_unicell_other_turb_eng_ody.Fm page 1 of 4 • adjust the ph of preserved samples to between ph 2–10 before testing. • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel, followed by a dry one. • use the standard solution or standard additi...

  • Page 740: Sulfate

    Sulfate sulfate page 2 of 4 sulfate_unicell_other_turb_eng_ody.Fm interferences the ions listed in the following table have been individually tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. Sample collection, storage, and preservatio...

  • Page 741: Sulfate

    Sulfate sulfate sulfate_unicell_other_turb_eng_ody.Fm page 3 of 4 3. Touch ok to accept the default values for standard concentration, sample volume, and spike volumes. Touch edit to change these values. After values are accepted, the unspiked sample reading will appear in the top row. 4. Prepare th...

  • Page 742: Sulfate

    Sulfate © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 sulfate_unicell_other_turb_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the ha...

  • Page 743: Dr/2400

    Dr/2400 sulfate sulfate_unicell_turb_eng_ody.Fm page 1 of 4 • adjust the ph of preserved samples to between ph 2–10 before testing. • clean the outside of the vial before placing it in the instrument. Wipe with a damp towel, followed by a dry one. • use the standard solution or standard addition met...

  • Page 744: Sulfate

    Sulfate sulfate page 2 of 4 sulfate_unicell_turb_eng_ody.Fm interferences the ions listed in the following table have been individually tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. 5. Touch the timer icon. Touch ok . A 30 second r...

  • Page 745: Sulfate

    Sulfate sulfate sulfate_unicell_turb_eng_ody.Fm page 3 of 4 sample collection, storage, and preservation analyze samples within 3 hours after collection for best results. Samples may be stored up to 28 days at 4 °c (39 °f). Warm samples to room temperature before analysis. Accuracy check standard ad...

  • Page 746: Sulfate

    Sulfate © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 sulfate_unicell_turb_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach off...

  • Page 747: Dr/2400

    Dr/2400 sulfide sulfide_none_other_mlb_eng_ody.Fm page 1 of 4 • analyze samples immediately. Do not preserve for later analysis. • avoid excessive agitation of samples to minimize sulfide loss. • some sulfide loss may occur if dilution is necessary. • wipe the outside of sample cells before each ins...

  • Page 748: Sulfide

    Sulfide sulfide page 2 of 4 sulfide_none_other_mlb_eng_ody.Fm determining soluble sulfides determine soluble sulfides by centrifuging the sample in completely filled, capped tubes and analyzing the supernatant. Insoluble sulfides are then estimated by subtracting the soluble sulfide concentration fr...

  • Page 749: Sulfide

    Sulfide sulfide sulfide_none_other_mlb_eng_ody.Fm page 3 of 4 interferences sample collection, storage, and preservation collect samples in clean plastic or glass bottles. Fill completely and cap tightly. Avoid excessive agitation or prolonged exposure to air. Analyze samples immediately. Method per...

  • Page 750: Sulfide

    Sulfide © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 sulfide_none_other_mlb_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach o...

  • Page 751: Dr/2400

    Dr/2400 sulfite sulfite_other_eng_ody.Fm page 1 of 4 • samples must be analyzed immediately. • sample and reagent temperature must be between 15–25 °c (59–77 °f). • adjust the sample ph between 3–10. Sulfite colorimetric method* * reagent sets for this method are only available in europe. (0.10 to 5...

  • Page 752: Sulfite

    Sulfite sulfite page 2 of 4 sulfite_other_eng_ody.Fm interferences sample collection, storage, and preservation collect samples in clean plastic or glass bottles. Samples may be stored up to 7 days by cooling to 4 °c (39 °f) or lower. Warm to 15–25 °c (59–77 °f) before analysis. 5. Add 2 drops of su...

  • Page 753: Sulfite

    Sulfite sulfite sulfite_other_eng_ody.Fm page 3 of 4 accuracy check standard additions method (sample spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Touch options . Touch standard additions . A summary of the standard additions procedure will appe...

  • Page 754: Sulfite

    Sulfite © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 sulfite_other_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or ...

  • Page 755: Dr/2400

    Dr/2400 surfactants, anionic (detergents) detergents_none_other_cvi_eng_ody.Fm page 1 of 4 • use benzene only in a well-ventilated area. • to prevent water droplets from forming in the sample cells, use only dry sample cells and discard the first few ml of benzene. Additionally, it helps to transfer...

  • Page 756: Zero

    Surfactants, anionic (detergents) surfactants, anionic (detergents) page 2 of 4 detergents_none_other_cvi_eng_ody.Fm 5. Add 30 ml of benzene to the funnel. Stopper the funnel and shake gently for one minute. 6. Place the separatory funnel in a support stand. 7. Touch the timer icon. Touch ok . A 30-...

  • Page 757

    Surfactants, anionic (detergents) surfactants, anionic (detergents) detergents_none_other_cvi_eng_ody.Fm page 3 of 4 interferences sample collection, storage, and preservation collect samples in clean plastic or glass bottles. Analyze samples as soon as possible, but they may be stored at least 24 h...

  • Page 758

    Surfactants, anionic (detergents) © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 detergents_none_other_cvi_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 80...

  • Page 759: Dr/2400

    Dr/2400 suspended solids suspendedsolids_none_other_pho_eng_ody.Fm page 1 of 2 suspended solids method 8006 photometric method* * adapted from sewage and industrial wastes, 31, 1159 (1959). (0 to 750 mg/l) scope and application: for water and wastewater 1. Touch hach programs . Select program 630 su...

  • Page 760: Suspended Solids

    Suspended solids © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 2 of 2 suspendedsolids_none_other_pho_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 c...

  • Page 761: Dr/2400

    Dr/2400 tannin and lignin tanninlig_none_other_tyr_eng_ody.Fm page 1 of 4 • filter turbid samples and report results as mg/l soluble tannic acid. • results will be given in mg/l tannins (as tannic acid). • for best accuracy, use a pipet to add the tanniver ® 3 solution. Tannin and lignin method 8193...

  • Page 762: Tannin and Lignin

    Tannin and lignin tannin and lignin page 2 of 4 tanninlig_none_other_tyr_eng_ody.Fm interferences sample collection, storage, and preservation collect samples in clean plastic or glass bottles. 5. Pipet 5.0 ml of sodium carbonate solution into each cell. Swirl to mix. A blue color will develop if ta...

  • Page 763: Tannin and Lignin

    Tannin and lignin tannin and lignin tanninlig_none_other_tyr_eng_ody.Fm page 3 of 4 accuracy check standard solution method prepare a 200-mg/l tannic acid standard solution as follows: 1. Dissolve 0.200 grams of tannic acid in deionized water and dilute to 1000 ml. Prepare this solution monthly. Fur...

  • Page 764: Tannin and Lignin

    Tannin and lignin © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 tanninlig_none_other_tyr_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contac...

  • Page 765: Dr/2400

    Dr/2400 toxicity toxicity_none_other_eng_ody.Fm page 1 of 6 • do not leave the cells in the instrument during incubation. All samples and control cells should be allowed to react under similar conditions of temperature and light. Toxicity method 10017 toxtrak™ method * ** * u.S. Pat. No. 5,413,916 *...

  • Page 766: Toxicity

    Toxicity toxicity page 2 of 6 toxicity_none_other_eng_ody.Fm 1. Inoculate a lauryl tryptose broth tube with an e. Coli aqua qc-stik™ according to the instructions that come with the stick. 2. Incubate the lauryl tryptose broth tube at 35 °c (95 °f) until the medium is visibly turbid (approximately 1...

  • Page 767: Toxicity

    Toxicity toxicity toxicity_none_other_eng_ody.Fm page 3 of 6 5. Touch zero . The display will show: 0.000 abs 6. Label a cell “control”. Open one toxtrak reagent powder pillow and add the contents to the empty reaction cell. 7. For each sample or dilution, repeat step 6. Label each cell. 8. Add 5.0 ...

  • Page 768: Toxicity

    Toxicity toxicity page 4 of 6 toxicity_none_other_eng_ody.Fm 13. Repeat step 12 for all samples and dilutions. Be sure to record each absorbance. 14. Allow the solutions in the tubes to react until the absorbance of the control decreases 0.60 ±0.10 abs. This takes 45–75 minutes. Invert occasionally....

  • Page 769: Toxicity

    Toxicity toxicity toxicity_none_other_eng_ody.Fm page 5 of 6 interpreting results the % inhibition results obtained are only a relative measurement. They do not represent a true quantitative measurement of toxic concentration. The % inhibition does not necessarily increase in direct proportion to th...

  • Page 770: Toxicity

    Toxicity © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 6 of 6 toxicity_none_other_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach off...

  • Page 771: Dr/2400

    Dr/2400 tph (total petroleum hydrocarbons) tphsoil_none_other_imm_eng_ody.Fm page 1 of 12 this tph test can be used for both soil and water testing. When testing soil, purchase the necessary items (see required apparatus on page 12) and perform the soil extraction procedure. When testing water sampl...

  • Page 772

    Tph (total petroleum hydrocarbons) tph (total petroleum hydrocarbons) page 2 of 12 tphsoil_none_other_imm_eng_ody.Fm 1. Weigh out 10 g of soil in the plastic weighing boat. 2. Carefully pour the soil into an extraction vial. 3. Use the 5-gram scoop to add one scoop of sodium sulfate to the extractio...

  • Page 773

    Tph (total petroleum hydrocarbons) tph (total petroleum hydrocarbons) tphsoil_none_other_imm_eng_ody.Fm page 3 of 12 1. Touch single wavelength then touch the λ button. Type in 450 nm and touch ok. Note: use the 1-cm square cell holder for this procedure. 2. Label an antibody cuvette for each calibr...

  • Page 774

    Tph (total petroleum hydrocarbons) tph (total petroleum hydrocarbons) page 4 of 12 tphsoil_none_other_imm_eng_ody.Fm 5. If testing soil: pipet 0.5 ml of diluent solution into each sample cuvette. If testing water: pipet 0.5 ml of each water sample into the appropriate cuvette. Note: use a new pipett...

  • Page 775

    Tph (total petroleum hydrocarbons) tph (total petroleum hydrocarbons) tphsoil_none_other_imm_eng_ody.Fm page 5 of 12 13. Wash each cuvette forcefully and thoroughly four times with deionized water. Empty the rinse water into the waste container. Note: ensure most of the water is drained from the cuv...

  • Page 776: Zero

    Tph (total petroleum hydrocarbons) tph (total petroleum hydrocarbons) page 6 of 12 tphsoil_none_other_imm_eng_ody.Fm measuring the color 5. Label and fill a zeroing cuvette with deionized water. Wipe the outside of all the cuvettes with a tissue to remove water, smudges, and fingerprints. 6. Install...

  • Page 777

    Tph (total petroleum hydrocarbons) tph (total petroleum hydrocarbons) tphsoil_none_other_imm_eng_ody.Fm page 7 of 12 using the wiretrol ® pipet the wiretrol pipet can accurately measure small quantities of liquids. It consists of two parts: a teflon ® -tipped plunger and a calibrated capillary tube....

  • Page 778

    Tph (total petroleum hydrocarbons) tph (total petroleum hydrocarbons) page 8 of 12 tphsoil_none_other_imm_eng_ody.Fm using the 1-cm microcuvette rack this rack (see figure 2) has been designed specifically to aid in achieving precise and accurate results when using the immunoassay technique to analy...

  • Page 779

    Tph (total petroleum hydrocarbons) tph (total petroleum hydrocarbons) tphsoil_none_other_imm_eng_ody.Fm page 9 of 12 sample #2: 0.400 abs sample #3: 0.550 abs interpretation interpretation for a soil sample: sample #1 — sample reading is less than the readings for both calibrators. Therefore the sam...

  • Page 780

    Tph (total petroleum hydrocarbons) tph (total petroleum hydrocarbons) page 10 of 12 tphsoil_none_other_imm_eng_ody.Fm diluting water samples higher concentrations in water can be tested by first diluting the sample with deionized water (see sensitivity). Test for other tph compounds (i.E., gasoline)...

  • Page 781

    Tph (total petroleum hydrocarbons) tph (total petroleum hydrocarbons) tphsoil_none_other_imm_eng_ody.Fm page 11 of 12 for example: if a 0.5 ml water sample is diluted to 50 ml and tested, the calibrator levels for diesel fuel in water would represent 200, 500, 1000, and 2000 ppm respectively. Sample...

  • Page 782

    Tph (total petroleum hydrocarbons) © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 12 of 12 tphsoil_none_other_imm_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 80...

  • Page 783: Dr/2400

    Dr/2400 volatile acids volatileaci_none_other_esr_eng_ody.Fm page 1 of 6 volatile acids method 8196 esterification method* * adapted from the analyst, 87, 949 (1962) (27 to 2800 mg/l) scope and application: for digestor sludges 1. Touch hach programs . Select program 770 volatile acids . Touch start...

  • Page 784: Volatile Acids

    Volatile acids volatile acids page 2 of 6 volatileaci_none_other_esr_eng_ody.Fm 9. When the timer beeps, cool the solutions to 25 °c (until the cell feels cold) with running tap water. 10. Using a pipet filler, pipet 0.5 ml of hydroxylamine hydrochloride solution into each cell. Swirl to mix. 11. Us...

  • Page 785: Volatile Acids

    Volatile acids volatile acids volatileaci_none_other_esr_eng_ody.Fm page 3 of 6 sample collection, preservation, and storage collect samples in clean plastic or glass bottles. Analyze as soon as possible after collection. Samples can be stored for up to 24 hours by cooling to 4 °c (40 °f) or below. ...

  • Page 786: Volatile Acids

    Volatile acids volatile acids page 4 of 6 volatileaci_none_other_esr_eng_ody.Fm standard solution method prepare a 500 mg/l volatile acid standard solution as follows: 1. Pipet 4.00 ml of a 62,500-mg/l volatile acid standard solution into a 500-ml class a volumetric flask. Dilute to volume with deio...

  • Page 787: Volatile Acids

    Volatile acids volatile acids volatileaci_none_other_esr_eng_ody.Fm page 5 of 6 required reagents quantity required description per test unit cat. No. Volatile acids reagent set (90 tests) .............................................................................................................22...

  • Page 788

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 789: Dr/2400

    Dr/2400 zinc zinc_pp_other_znc_eng_ody.Fm page 1 of 6 • caution! Zincover ® 5 reagent contains cyanide and is very poisonous if taken internally or if fumes are inhaled. Do not add to an acidic sample (ph • use only glass-stoppered cylinders in this procedure. • wash glassware with 1:1 hcl (cat. No....

  • Page 790: Zinc

    Zinc zinc page 2 of 6 zinc_pp_other_znc_eng_ody.Fm 5. Pour 10 ml of the solution into a sample cell (this is theblank). 6. Use a plastic dropper to add 0.5 ml of cyclohexanone to the remaining solution in the graduated cylinder. 7. Touch the timer icon touch ok . A 30-second reaction period will beg...

  • Page 791: Zinc

    Zinc zinc zinc_pp_other_znc_eng_ody.Fm page 3 of 6 interferences samples containing amino-tri(methylene phosphonic acid) (amp) will exhibit a negative interference. Perform a total phosphorus digestion (method 8190) to eliminate this interference. Important: adjust the ph of the sample after the tot...

  • Page 792: Zinc

    Zinc zinc page 4 of 6 zinc_pp_other_znc_eng_ody.Fm 7. After completing the sequence, touch graph to view the best-fit line through the standard additions data points, accounting for matrix interferences. Touch view: fit , then select ideal line and touch ok to view the relationship between the sampl...

  • Page 793: Zinc

    Zinc zinc zinc_pp_other_znc_eng_ody.Fm page 5 of 6 summary of method zinc and other metals in the sample are complexed with cyanide. Adding cyclohexanone causes a selective release of zinc. The zinc then reacts with 2-carboxy-2'-hydroxy-5'-sulfoformazyl benzene (zincon) indicator to form a blue-colo...

  • Page 794

    Hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach office or distributor serving you. Www.Hach.Com techhelp@hach.Com in the u.S.A. – outside the u.S.A. – on the worldwide web – ; e...

  • Page 795: Dr/2400

    Dr/2400 zinc zinc_unicell_other_par_eng_ody.Fm page 1 of 4 • samples collected in glass or plastic bottles should be analyzed as quickly as possible. • adjust ph of preserved samples to between ph 3–5 with 1.0 n sulfuric acid standard solution before analysis. • clean the outside of the vial before ...

  • Page 796: Zinc

    Zinc zinc page 2 of 4 zinc_unicell_other_par_eng_ody.Fm interferences the ions listed in the following table have been individually tested up to the given concentrations. Cumulative effects and the influence of other ions have not been evaluated. 5. Invert the sample vial repeatedly until the solids...

  • Page 797: Zinc

    Zinc zinc zinc_unicell_other_par_eng_ody.Fm page 3 of 4 sample collection, preservation, and storage collect samples in acid-cleaned glass or plastic containers. No acid addition is necessary if analyzing the samples immediately. To preserve samples, adjust the ph to 2 or less with concentrated nitr...

  • Page 798: Zinc

    Zinc © hach company, 2003. All rights reserved. Printed in the u.S.A. 3/04 1ed page 4 of 4 zinc_unicell_other_par_eng_ody.Fm hach company world headquarters telephone: (970) 669-3050 fax: (970) 669-2932 for technical assistance, price information and ordering: call 800-227-4224 contact the hach offi...