Labomed Lx 400 User Manual - page 20

17

“Vernier Scales”

These scales allow for easy identification of the specimen’s position 

(coordinates), making it easy to return to a particular region of interest 

after scanning the slide. (Figure 23.)

1. The horizontal coordinate can be read at position (1) on the specimen 

holder.

2. The vertical coordinate can be read at the index line (2).

Focusing Procedure (Figure 18)

1. Rotate the coarse adjustment knob (1) clockwise so that the objective 

    (3) is as close as possible to the specimen (We recommend starting with   

    10X).

2. While observing the specimen through the eyepieces, slowly rotate the 

    coarse adjustment knob (1)  counterclockwise to lower the stage.

3. When coarse focusing of the specimen is obtained (an image is 

    observed), rotate the fine adjustment knob (2) for fine detail focusing.

Working Distance (WD)

The WD refers to the distance between each objective and the specimen, 

when acute focus of the specimen is obtained.

3

Adjusting the Focus

Objective 

Magnification

4X

10X

40X

100X

WD (mm)

30.5

4.82

0.55

0.11

Cover glass

This is the glass plate placed on the specimen. For optimum optical 

performance, the cover glass thickness, which is the distance from its 

surface to the specimen surface, should be 0.17 mm.

Slide glass

This glass plate should ideally have a length of 76 mm, width of 26 mm 

±1 mm and thickness between 0.9 and 1.4 mm.

Cover glass

Slide glass

Fig. 23

Fig. 24

Fig. 25

1

2

1

2

3

WD