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LaMotte smart spectro 2 Operator's Manual

Manual is about: spectrophotometer

Summary of smart spectro 2

  • Page 1

    Operator’s manual 2 2000-02-mn 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 2: Contents

    Contents general information  packaging & delivery ............................................................................................................... 4  general precautions ...................................................................................................................

  • Page 3

    Initialization 45 programmed tests  introduction ............................................................................................................................. 46  sequences of tests .......................................................................................................

  • Page 4: General Information

    4 smart spectro 2 operator’s manual 04.16 general information  packaging & delivery experienced packaging personnel at lamotte company assure adequate protection against normal hazards encountered in transportation of shipments. After the product leaves the manufacturer, all responsibility for its ...

  • Page 5

    Smart spectro 2 operator’s manual 04.16 5  limits of liability under no circumstances shall lamotte company be liable for loss of life, property, profi ts, or other damages incurred through the use or misuse of their products.  warranty lamotte company warrants this instrument to be free of defects...

  • Page 6

    6 smart spectro 2 operator’s manual 04.16  specifications instrument type: single beam spectrophotometer wavelength range 350-1000 nm optical system single beam, grating 1200 lines/mm wavelength accuracy and repeatability ± 2 nm photometric accuracy ± 0.5%t bandwidth 4 nm transmittance range 0-125%...

  • Page 7

    Smart spectro 2 operator’s manual 04.16 7 3. Skoog, d.A., west, d. M., fundamental of analytical chemistry, 2nd ed., holt rinehart and winston, inc, 1969, p. 26. Accuracy: accuracy is the nearness of a measurement to the accepted or true value.4 the accuracy can be expressed as a range, about the tr...

  • Page 8

    8 smart spectro 2 operator’s manual 04.16  contents and accessories contents smart spectro 2 spectrophotometer power cable test tubes, with caps ac adapter sample cell holder, universal smart spectro 2 quick start guide sample cell holder, 10 mm square smart spectro 2 manual note: the battery pack ...

  • Page 9: Chemical Testing

    Smart spectro 2 operator’s manual 04.16 9 chemical testing  water sampling for chemical analysis taking representative samples the underlying factor to be considered for any type of water sampling is whether or not the sample is truly representative of the source. To properly collect a representati...

  • Page 10

    10 smart spectro 2 operator’s manual 04.16 fi ve times and it may be raised to the surface for examination. The inner chamber of the sampling device is lifted out and portions of the water sample are carefully dispensed for subsequent chemical analysis. A snap-plunger water sampler (lamotte code 1077...

  • Page 11

    Smart spectro 2 operator’s manual 04.16 11  an introduction to colorimetric analysis & spectroscopy most test substances in water are colorless and undetectable to the human eye. To test for their presence we must fi nd a way to “see” them. The lamotte smart spectro 2 can be used to measure any test...

  • Page 12

    12 smart spectro 2 operator’s manual 04.16 produces a pink color proportional to the nitrate concentration in the sample (the greater the nitrate concentration, the darker the pink color). A wavelength in the green region should be selected to analyze this sample since a pinkish-red solution absorbs...

  • Page 13

    Smart spectro 2 operator’s manual 04.16 13 sample color wavelength range yellow 350-450 yellow-orange 450-490 orange 490-510 pink 510-570 red 570-600 green and blue 600-750  calibration curves the smart spectro 2 contains precalibrated tests for the lamotte reagent systems. The fi rst step in using ...

  • Page 14

    14 smart spectro 2 operator’s manual 04.16 concentration, simple linear graph paper can be used. Plot the standard solution concentration on the horizontal axis, and the absorbance on the vertical axis. 6. After plotting the results, draw a line, or curve, of best fi t through the plotted points. The...

  • Page 15

    Smart spectro 2 operator’s manual 04.16 15  preparing dilute standard solutions standard solutions should be prepared to create a calibration curve. Standard solutions can be prepared by diluting a known concentrated standard by specifi ed amounts. A chart or computer spreadsheet can be created to d...

  • Page 16

    16 smart spectro 2 operator’s manual 04.16 for example, a 10.0 ml water sample was determined to contain 0.3 ppm iron. To a second 10.0 ml sample, 0.1 ml of 50 ppm iron standard was added. The concentration of iron due to the “spike” was (0.10 ml x 50 ppm)/10.0 ml = 0.50 ppm. The concentration of ir...

  • Page 17

    Smart spectro 2 operator’s manual 04.16 17  interferences lamotte reagent systems are designed to minimize most common interferences. Each individual test instruction discusses interferences unique to that test. Be aware of possible interferences in the water being tested. The reagent systems also ...

  • Page 18

    18 smart spectro 2 operator’s manual 04.16 operation of the smart spectro 2  overview the smart spectro 2 is a portable, microprocessor controlled, direct reading, single beam spectrophotometer. It has a 9 line liquid crystal display for alphabetical and numerical messages. The operation is control...

  • Page 19

    Smart spectro 2 operator’s manual 04.16 19  components figure 1 shows a diagram of the smart spectro 2 and the components. Power input socket: for 12v dc power input usb port 10mm square cell in adapter 25mm round cell in universal adapter display make selections scroll between choices scroll betwe...

  • Page 20

    20 smart spectro 2 operator’s manual 04.16  quick start 1. Press on . The lamotte logo screen will appear for about 2 seconds. After initialization the main menu will appear. Main menu 12:00:00 programmed tests %t/abs pc link system setup 2. Press or to scroll to programmed tests . Main menu 12:00:...

  • Page 21

    Smart spectro 2 operator’s manual 04.16 21 4. Press or to scroll to all tests . Programmed tests 546 nm sequence 1 sequence 2 sequence 3 all tests edit 5. Press *enter to select all tests. All tests 546 nm 1 alkalinity-udv 2 aluminum 3 ammonia-n l f 4 ammonia-n l s 5 ammonia-n h 6 arsenic 7 biguanid...

  • Page 22

    22 smart spectro 2 operator’s manual 04.16 7. Press *enter to select the test. 2 aluminum 535 nm scan blank scan sample test result list 8. Insert the blank. Close lid. Press *enter to scan the blank. Blanking will momentarily appear on the top line of the display. The blank has been stored. 2 alumi...

  • Page 23: General Operating Procedures

    Smart spectro 2 operator’s manual 04.16 23 general operating procedures the operation of the smart spectro 2 is controlled by a microprocessor. The microprocessor is programmed with menu driven software. A menu is a list of choices. This allows a selection of various tasks for the spectrophotometer ...

  • Page 24

    24 smart spectro 2 operator’s manual 04.16  the display & the menus the display allows menu selections to be viewed and chosen. These choices instruct the spectrophotometer to perform specifi c tasks. The menus are viewed in the display using a general format which is followed from one menu to the n...

  • Page 25: System Setup

    Smart spectro 2 operator’s manual 04.16 25 testing menu testing menu testing menu first choice second choice another second choice another and another another and another and another and another and another and another and another and another and another and another and another and another and anoth...

  • Page 26

    26 smart spectro 2 operator’s manual 04.16  calibrate wl the calibrate wavelength (calibrate wl) mode is used to establish or re-establish the accuracy of the wavelength selection process. Normally, the calibrate wavelength procedure should be run after the smart spectro 2 is turned on and allowed ...

  • Page 27

    Smart spectro 2 operator’s manual 04.16 27 3. Press *enter to select system setup. System setup dark current calibrate wl bt printer edit clock store method beeper on/off correction wl language 4. Press or to scroll to calibrate wl . System setup dark current calibrate wl bt printer edit clock store...

  • Page 28

    28 smart spectro 2 operator’s manual 04.16 6. Press or to highlight no or yes. Calibrate wl calibrate wl??? Please select no 7. Press *enter to begin the wavelength calibration. Calibrate wl calibrate wl??? Please select yes during the calibration, the spectro will display progress updates on the to...

  • Page 29

    Smart spectro 2 operator’s manual 04.16 29 1. Turn on mobile bluetooth printer (code 5-0066). Main menu 12:00:00 programmed tests %t/abs pc link system setup 2. Press on to turn on the smart spectro 2. The lamotte logo screen will appear for about 2 seconds. After initialization the main menu will a...

  • Page 30

    30 smart spectro 2 operator’s manual 04.16 4. Press *enter to select system setup. System setup dark current calibrate wl bt printer edit clock store method beeper on/off correction wl language 5. Press or to scroll to bt printer. System setup dark current calibrate wl bt printer edit clock store me...

  • Page 31

    Smart spectro 2 operator’s manual 04.16 31 7. First time pairing of printer and spectro to pair the mobile spin printer to the smart spectro 2 for the fi rst time, scroll to scan for devices. If the printer and smart spectro 2 have already been paired, go to page 32. Press *enter to select scan for d...

  • Page 32

    32 smart spectro 2 operator’s manual 04.16 7. Printer and spectro already paired if the printer and spectro have already been paired, scroll to connect. Press *enter to select connect. Bt printer connect disconnect scan for devices if the connection to the mobile bluetooth printer is successful, con...

  • Page 33

    Smart spectro 2 operator’s manual 04.16 33  edit clock the clock information is used to time stamp the data points in the data logger. 1. Press on . The lamotte logo screen will appear for about 2 seconds. After initialization the main menu will appear. Main menu 12:00:00 programmed tests %t/abs pc...

  • Page 34

    34 smart spectro 2 operator’s manual 04.16 4. Press or to scroll to edit clock . System setup dark current calibrate wl bt printer edit clock store method beeper on/off correction wl language 5. Press *enter to select edit clock. The display will show seconds-minutes- hours-day-month-year. Note: hou...

  • Page 35

    Smart spectro 2 operator’s manual 04.16 35 7. Press *enter to save the second setting. Edit clock 46:13:10 14-04-2016 set second: 15 8. Follow the procedure to set the minutes, hour, month, day, and year. Press exit to return to the main menu. System setup dark current calibrate wl bt printer edit c...

  • Page 36

    36 smart spectro 2 operator’s manual 04.16 2. Press or to scroll to system setup . Main menu 12:00:00 programmed tests %t/abs pc link system setup 3. Press *enter to select system setup. System setup dark current calibrate wl bt printer edit clock store method beeper on/off correction wl language 4....

  • Page 37

    Smart spectro 2 operator’s manual 04.16 37 5. Press *enter to select store method. Store method automatic manual end of list 6. Press or to select the desired store method. In the auto method, all results will automatically be stored in the data logger. This is the default setting. In the manual met...

  • Page 38

    38 smart spectro 2 operator’s manual 04.16  beeper on/off if the beeper function is turned on, the beeper will sound at each button push. The default beeper setting is on. 1. Press on . The lamotte logo screen will appear for about 2 seconds. After initialization the main menu will appear. Main men...

  • Page 39

    Smart spectro 2 operator’s manual 04.16 39 4. Press or to scroll to beeper on/off . System setup dark current calibrate wl bt printer edit clock store method beeper on/off correction wl language 5. Press *enter to select beeper on/off. Beeper on/off on off 6. Press or to highligh on or off. Beeper o...

  • Page 40

    40 smart spectro 2 operator’s manual 04.16 7. Press *enter to select on or off. Beeper on/off on off 8. Press exit to return to the system setup. System setup dark current calibrate wl bt printer edit clock store method beeper on/off correction wl language  correction wl the correction wl feature s...

  • Page 41

    Smart spectro 2 operator’s manual 04.16 41  language there are two choices for language: english and chinese. The default language is english. 1. Press on . The lamotte logo screen will appear for about 2 seconds. After initialization the main menu will appear. Main menu 12:00:00 programmed tests %...

  • Page 42

    42 smart spectro 2 operator’s manual 04.16 4. Press or to scroll to language . System setup dark current calibrate wl bt printer edit clock store method beeper on/off correction wl language 5. Press *enter to select language. Language english chinese 6. Press or to scroll to highlight english or chi...

  • Page 43

    Smart spectro 2 operator’s manual 04.16 43 7. Press *enter to save the selection and return to the system setup. System setup dark current calibrate wl bt printer edit clock store method beeper on/off correction wl language  reset to default the smart spectro 2 can be reset to the default settings....

  • Page 44

    44 smart spectro 2 operator’s manual 04.16 3. Press *enter to select system setup. System setup dark current calibrate wl bt printer edit clock store method beeper on/off correction wl language 4. Press or to scroll to end of list . System setup calibrate wl bt printer edit clock store method beeper...

  • Page 45: Initialization

    Smart spectro 2 operator’s manual 04.16 45 6. When the default reset is complete the display will return to the system setup menu. System setup calibrate wl bt printer edit clock store method beeper on/off correction wl language end of list initialization when on is pressed to turn the spectrophomet...

  • Page 46: Programmed Tests

    46 smart spectro 2 operator’s manual 04.16 programmed tests  introduction the programmed tests mode is used to run all lamotte pre-programmed tests and user tests. This is also where user tests and sequences are set-up and edited. 1. Press on . The lamotte logo screen will appear for about 2 second...

  • Page 47

    Smart spectro 2 operator’s manual 04.16 47  sequences of tests sequence 1 , sequence 2, and sequence 3 are alterable sequences. They may be edited using the edit function mode. Any of the lamotte pre-programmed tests or user tests may be placed in these sequences in whatever testing order that is p...

  • Page 48

    48 smart spectro 2 operator’s manual 04.16  general testing procedures the following are some step by step examples of how to run tests from the programmed tests menu. These test procedures are designed to be used with lamotte smart spectro 2 reagent systems. Testing with the programmed tests 1. Pr...

  • Page 49

    Smart spectro 2 operator’s manual 04.16 49 4. Press or to scroll to all tests . Programmed tests sequence 1 sequence 2 sequence 3 all tests edit 5. Press *enter to select all tests. All tests 1 alkalinity-udv 2 aluminum 3 ammonia-n l f 4 ammonia-n l s 5 ammonia-n h 6 arsenic 7 biguanide 8 boron 6. P...

  • Page 50

    50 smart spectro 2 operator’s manual 04.16 7. Press *enter to select the test. 2 aluminum 535 nm scan blank scan sample test result list 8. Insert the blank. Close lid. Press *enter to scan the blank. Blanking will momentarily appear on the top line of the display. The blank has been stored. 2 alumi...

  • Page 51

    Smart spectro 2 operator’s manual 04.16 51 10. Press *enter to store the result to the test resultl list. If store method is automatic, the test result will be stored automatically. To repeat the test, press exit to escape the test screen, then press *enter to scan the sample again. The last blank s...

  • Page 52

    52 smart spectro 2 operator’s manual 04.16 2. Press *enter to select edit. Edit 535nm edit sequence 1 edit sequence 2 edit sequence 3 edit user tests edit  adding or deleting tests there are two ways to alter a sequence - insert and delete. Insert is used to add a new test to a sequence and to plac...

  • Page 53

    Smart spectro 2 operator’s manual 04.16 53 2. Press *enter to select the sequence to be edited. Edit sequence 3 535nm 3. Press *enter to view the list of editing options. Edit sequence 3 535nm insert delete 4. Press *enter to select insert to insert a test into the sequence. Insert 1 alkalinity-udv ...

  • Page 54

    54 smart spectro 2 operator’s manual 04.16 5. Press or to scroll to the desired test. Insert 535nm 1 alkalinity-udv 2 aluminum 3 ammonia-n l f 4 ammonia-n l s 5 ammonia-n h 6 arsenic 7 biguanide 8 boron 6. Press *enter to select the test. Edit sequence 3 535nm 2 aluminum 7. Press *enter to save the ...

  • Page 55

    Smart spectro 2 operator’s manual 04.16 55 8. Press *enter to select another test to add to the sequence. The second test will be added above the fi rst in the sequence. Insert 535nm 1 alkalinity-udv 2 aluminum 3 ammonia-n l f 4 ammonia-n l s 5 ammonia-n h 6 arsenic 7 biguanide 8 boron 9. Press or to...

  • Page 56

    56 smart spectro 2 operator’s manual 04.16 below is an example of how to delete a test from sequence 3, which was just created, starting from the edit menu. 1. Press to scroll to the sequence to be edited. Edit 535nm edit sequence 1 edit sequence 2 edit sequence 3 edit user tests 2. Press *enter to ...

  • Page 57

    Smart spectro 2 operator’s manual 04.16 57 4. Press *enter to select the test to be deleted. Edit sequence 3 535nm insert delete 2 aluminum 5. Press or to scroll to delete. Edit sequence 3 535nm insert delete 2 aluminum 6. Press *enter to select delete. Sequence 3 has now been modifi ed and will rema...

  • Page 58

    58 smart spectro 2 operator’s manual 04.16  edit user tests if a test other than the lamotte programmed tests is performed regularly, a calibration for it may be entered in one of the 25 user tests. These tests are originally named “user test 1 - 25”. It will be possible to rename the test, select ...

  • Page 59

    Smart spectro 2 operator’s manual 04.16 59 2. Press *enter to select edit. Edit 535nm edit sequence 1 edit sequence 2 edit sequence 3 edit user tests 3. Press to scroll to edit user tests. Edit 535nm edit sequence 1 edit sequence 2 edit sequence 3 edit user tests 4. Press *enter to select edit user ...

  • Page 60

    60 smart spectro 2 operator’s manual 04.16 5. Press or to scroll to the user test to be edited. Edit user test 535nm 101 user test 1 102 user test 2 103 user test 3 104 user test 4 105 user test 5 106 user test 6 107 user test 7 108 user test 8 6. Press *enter to select the user test to be edited. N...

  • Page 61

    Smart spectro 2 operator’s manual 04.16 61 1. The user test to be edited has been selected. 102 user test 2 535nm name the test select wl new calibration format result 2. Press *enter to select name the test. 102 user test 2 535nm name the test select wl new calibration format result name: 102 ! A b...

  • Page 62

    62 smart spectro 2 operator’s manual 04.16 4. Press *enter to select the highlighted character. The character will appear next to the test number. 102 user test 2 535nm name the test select wl new calibration format result name: 102 h ! A b c d e f g h i j k l m n o p q r s t u v w x y z 0 1 2 3 4 5...

  • Page 63

    Smart spectro 2 operator’s manual 04.16 63 2. Press *enter to select select wl. 102 h2o 535nm name the test select wl new calibration format result please select wl = 535 3. Press or to scroll to desired wavelength. 102 h2o 535nm name the test select wl new calibration format result please select wl...

  • Page 64

    64 smart spectro 2 operator’s manual 04.16  entering a new calibration 1. Press to scroll to new calibration. 102 h2o 465nm name the test select wl new calibration format result 2. Press *enter to select new calibration . 102 h2o 465nm name the test select wl new calibration format result input dat...

  • Page 65

    Smart spectro 2 operator’s manual 04.16 65 4. Press *enter to save the selection. New calibration 465nm enter std. 1 enter std. 2 enter std. 3 enter std. 4 calculate 5. Press *enter to select the fi rst data point. Enter std. 1 465nm scan blank std. 1 = ! 0 1 2 3 4 5 6 7 8 9 . 6. Place a blank in the...

  • Page 66

    66 smart spectro 2 operator’s manual 04.16 7. Place the reacted standard of the lowest concentration in the chamber. Press or to highlight each character in the concentration value. Press *enter after each selection to confi rm the selection. Enter std. 1 465nm blanked std. 1 = ! 0 1 2 3 4 5 6 7 8 9 ...

  • Page 67

    Smart spectro 2 operator’s manual 04.16 67 10. Press *enter to move to the second standard. Use the same procedure to scan the blank and then scan the second standard to create a second data point. Repeat the procedure until the data from all of the standards has been entered into the calibration. N...

  • Page 68

    68 smart spectro 2 operator’s manual 04.16 chosen. 1. Degree calculates the best straight line fi t but without forcing the line through the origin. The minimum number of standards needed for a calibration is one for 1. Degree thru.0 and two for 1. Degree. Note: 2. Degree and 3. Degree calculations a...

  • Page 69

    Smart spectro 2 operator’s manual 04.16 69 1. Press to scroll to format result. 102 h2o 465nm name the test select wl new calibration format result 2. Press *enter to select format result. Decimal places 465nm 0 places 1 places 2 places 3 places 3. Press or to select the number of decimal places to ...

  • Page 70: Measuring In The %t/abs Mode

    70 smart spectro 2 operator’s manual 04.16 4. Press *enter to select the number format. 102 h2o 465nm name the test select wl new calibration format result 5. Press exit to return to edit user test menu. Press exit again to escape to edit menu and again to return to the programmed test menu. Note: t...

  • Page 71

    Smart spectro 2 operator’s manual 04.16 71 2. Press or to scroll to %t/abs . Main menu 12:00:00 programmed tests %t/abs pc link system setup 3. Press *enter to select %t/abs. %t/abs 465nm select wl scan blank scan sample test result list end of list 4. Press *enter to select select wl. %t/abs 465nm ...

  • Page 72

    72 smart spectro 2 operator’s manual 04.16 5. Press or to scroll to desired wavelength. %t/abs 465nm select wl scan blank scan sample test result list end of list please select wl = 466 6. Press *enter to select the wavelength. The meter is ready to scan. %t/abs 466nm select wl scan blank scan sampl...

  • Page 73

    Smart spectro 2 operator’s manual 04.16 73 8. Insert the reacted sample into the chamber. Press *enter to scan the sample. The result will be displayed. %t/abs 466nm t = 79.5%t a = 0.0996a store press [enter] 9. Press *enter to store the result. Press exit to skip storing the result. Note: if automa...

  • Page 74: Store, Delete and Print Data

    74 smart spectro 2 operator’s manual 04.16 11. Press *enter to select test result list. %t/abs 466nm date-time wl abs %t *1309:22 546 0.0000 100.0 1309:02 546 0.2763 52.9 1308:55 466 0.0996 79.5 3013:58 570 1.2363 5.8 3013:37 520 1.7479 1.8 3013:37 430 1.4883 3.2 store, delete and print data  store...

  • Page 75

    Smart spectro 2 operator’s manual 04.16 75  delete data to delete all saved data: 1. Scroll to system setup on the main menu main menu 12:00:00 programmed tests %t/abs pc link system setup 2. Press *enter to select system setup. System setup dark current calibrate wl bt printer edit clock store met...

  • Page 76

    76 smart spectro 2 operator’s manual 04.16 4. Press *enter to select store method. Store method automatic manual end of list 5. Scroll to end of list . Store method automatic manual end of list 6. Press and hold *enter for 8 seconds to clear the memory. Warning: this will delete all of the saved dat...

  • Page 77: Pc Link

    Smart spectro 2 operator’s manual 04.16 77  print data data can be printed directly for a programmed tests scan, %t/abs scan, or from the test result list for programmed tests or test result list for %t/abs. If the store method is automatic and the printer is disconnected, the data point will autom...

  • Page 78: Battery Operation

    78 smart spectro 2 operator’s manual 04.16 battery operation the smart spectro 2 can be run using battery power. The battery pack consists of a rechargeable ni-metal hydride battery pack. The battery pack (code 2000-bp) and battery charger (code 2000-bc) are not included in the standard meter packag...

  • Page 79

    Smart spectro 2 operator’s manual 04.16 79 lamp bracket guiding pin (on the mounting wall) lamp bracket assembly mounted on the wall with screws (2) lamp bracket assembly installed lamp bracket installation guiding hole lamp bracket mounting screw (2) back view of the lamp bracket lamp-locking screw...

  • Page 80

    80 smart spectro 2 operator’s manual 04.16  halogen lamp replacement 1. Open the lamp access cover on the back of the instrument. 2. Loosen the lamp bracket mounting screws (2). Caregully remove the lamp bracket off the mounting guide pin. Note: do not pull too hard to avoid breaking the wire connec...

  • Page 81

    Smart spectro 2 operator’s manual 04.16 81 warning: do not overtighten the lamp-locking screws! It may cause damage to or even break the lamp. 5. Align the lamp bracket installation guiding hole to the guiding pin on the mounting wall. Us the screws to lock the bracket in place. Do not tighten the s...

  • Page 82

    82 smart spectro 2 operator’s manual 04.16  clock battery (ram battery) the smart spectro 2 date/clock function is powered by a cr 1220 battery. This battery should be replaced about every 8 years. If the date or clock function stops or if you receive the message “warning ram battery low” you shoul...

  • Page 83: Troubleshooting Guide

    Smart spectro 2 operator’s manual 04.16 83  meter disposal waste electrical and electronic equipment (weee) natural resources were used in the production of this equipment. This equipment may contain materials that are hazardous to health and the environment. To avoid harm to the environment and na...

  • Page 84

    84 smart spectro 2 operator’s manual 04.16 warning signal too strong this can be an indication that the meter was accidently shut-off during wavelength calibration. Shut meter off and restart. Warning ram battery low clock battery needs to be replaced soon. If it fails all user tests and data log data...

  • Page 85

    Test procedures 2 2000-02-mn 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 86

    Spectrophotometer reagent systems list call lamotte technical services at 1-800-344-3100 (410-778-3100 outside the usa) or email at tech@lamotte.Com for a current list of available calibrations and downloading instructions. Test factor (test #) range (ppm) mdl test method (# of reagents) # of tests ...

  • Page 87

    Copper-ddc (32) 0.00–6.00 0.05 diethyldithiocarbamate (1) 50 copper-udv (33) 0.00–4.00 0.20 bicinchoninic acid (1)† 100 cyanide (35) 0.00-0.50 0.50 pyridine-barbituric acid (5) 50 cyanuric acid (36) 0–200 16 melamine (1) 40 cyanuric acid-udv (37) 0–150 5 melamine (1)† 100 deha (037) see oxygen scave...

  • Page 88

    Nitrogen, total (62) 0-25 mg/l 2 mg/l chromotropic acid/digestion (6)* 25 oxygen scanvengers various various oxygen reduction (3) 50 ozone-low range (071) 0.00–0.40 0.02 indigo trisulfonate (3) 100 ozone-high range (072) 0.00–1.50 0.05 indigo trisulfonate (3) 20 ph-chlorophenol red (073) 5.0-7.0 – c...

  • Page 89: Alkalinity, Udv

    Test procedures alkalinity, udv unit dose vial method • code 4318-j quantity contents code 1 alkalinity unit dose vials, 20 pouches 4318-j equipment needed but not supplied: standard accessory package • code 1961 1 package of 3 vials (empty) 0156 1 syringe, 6 ml, plastic 1184 1 foil storage bag 9467...

  • Page 90

    Test procedures application: drinking and surface water and swimming pool water range: 0–200 ppm as caco 3 mdl: 15 ppm method: the sample is added to a buff ered indicator reagent. The color that develops, ranging from yellow to blue, will indicate the amount of alkalinity in the sample. Sample handl...

  • Page 91

    Test procedures procedure 1. Use 10 mm square cell adapter 2. Press on button to turn spectrophotometer on. 3. Scroll to and select programmed tests. 4. Scroll to and select all tests (or another sequence containing 1 alkalinity-udv ) from testing menu. 5. Scroll to and select 1 alkalinity-udv from ...

  • Page 92

    Test procedures alkalinity , udv smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 93: Aluminum

    Test procedures aluminum eriochrome cyanine r method • code 364i-01-sc quantity contents code 5 g * aluminum inhibitor reagent *7865-c 2 x 120 ml * aluminum buff er reagent *7866-j 120 ml aluminum indicator reagent 7867-j 15 ml aluminum complexing reagent 7868-e 1 spoon, 0.05 g, plastic 0696 2 pipets...

  • Page 94

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 2 aluminum ). 4. Scroll to and select 2 aluminum from menu. 5. Rinse a clean spectro tub...

  • Page 95: Ammonia-Nitrogen, Low Range

    Test procedures ammonia-nitrogen, low range salicylate method • code 3659-01-sc quantity contents code 60 ml *salicylate ammonia #1 *3978-h 10 g *salicylate #2 *7457-d 2 x 5 g *salicylate #3 reagent powder *7458-c 1 spoon, 0.1 g, plastic 0699 1 spoon, 0.15 g, plastic 0727 1 pipet, 1.0 ml, plastic 03...

  • Page 96

    Test procedures application: low concentrations of ammonia in fresh, brackish and salt water; fresh and salt water aquariums. Range: 0.00–1.00 ppm ammonia-nitrogen mdl: 0.02 ppm fresh waer 0.10 ppm salt water method: salicylate and ammonia react at high ph in the presence of a chlorine donor and an ...

  • Page 97

    Test procedures procedure–fresh water use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests from menu. 3. Scroll to and select all tests (or another sequence containing 3 ammonia-n l f) from testing menu. 4. Scroll to and select 3 ammo...

  • Page 98

    Test procedures to determine the percentage of ammonia-nitrogen that is unionized and ionized, consult the appendix. Procedure–salt water use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests from menu. 3. Scroll to and select all test...

  • Page 99

    Test procedures calculations: to express results as unionized ammonia (nh 3 ): ppm unionized ammonia (nh 3 ) = ppm ammonia-nitrogen (nh 3 –n) x 1.2 to express results as ionized ammonia (nh4): ppm ionized ammonia (nh 4+ ) = ppm ammonia-nitrogen (nh 3 –n) x 1.3 notes: for the best possible results, a...

  • Page 100

    Test procedures ammonia-nitrogen, low range smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 101: Ammonia-Nitrogen, High Range

    Test procedures ammonia-nitrogen, high range nesslerization method • code 3642-sc quantity contents code 30 ml ammonia nitrogen reagent #1 v-4797-g 2 x 30 ml *ammonia nitrogen reagent #2 *v-4798-g 1 pipet, 1 ml, plastic 0354 *warning: reagents marked with an * are considered to be potential health h...

  • Page 102

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 5 ammonia-n h ) from testing menu. 4. Scroll to and select 5 ammonia-n h from menu. 5. R...

  • Page 103: Biguanide

    Test procedures biguanide colorimetric method • code 4044 quantity contents code 2 x 60 ml biguanide indicator 3994-h 1 pipet, plastic, 1.0 ml 0354 *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data sheet (sds) for these reagents go to w...

  • Page 104

    Test procedures procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Select all tests (or another sequence containing 7 biguanide from testing menu . 4. Scroll to and select 7 biguanide from menu. 5. Rinse a tube (0290) w...

  • Page 105: Boron

    Test procedures boron azomethine-h method • code 4868-01 quantity contents code 120 ml *boron buff er *4869-j 10 g *boron indicator powder *4870-d 1 pipet, plastic, 1.0 ml 0354 1 spoon, 0.15 g 0727 1 dark storage chamber, brown 0108 *warning: reagents marked with an * are considered to be potential h...

  • Page 106

    Test procedures procedure use universal sample holder 1. This test requires a reagent blank. Rinse a tube (0290) with clear, colorless, boron free water. Fill to 10 ml line with clear, colorless, boron free water. 2. Use the 1.0 ml pipet (0354) to add 2 ml of *boron buff er (4869). Cap and mix. 3. Us...

  • Page 107: Bromine

    Test procedures bromine dpd tablet method • code 3643-sc quantity contents code 100 dpd #1 instrument grade tablets 6903a-j 100 dpd #3 instrument grade tablets 6197a-j 15 ml glycine solution 6811-e *warning: reagents marked with an * are considered to be potential health hazards. To view or print a ...

  • Page 108

    Test procedures procedure a: bromine (no chlorine) use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 9 bromine-lr ) from testing menu. 4. Scroll to and select 9 bro...

  • Page 109: Bromine, Udv

    Test procedures bromine, udv dpd unit dose vial method • code 4311-j quantity contents code 1 *free chlorine unit dose vials, 20 pouches *4311-j equipment needed but not supplied: standard accessory package • code 1961 1 package of 3 vials (empty) 0156 1 syringe, 3 ml, plastic 1184 1 foil storage ba...

  • Page 110

    Test procedures application: drinking, surface, and saline waters; domestic and industrial waters and wastes. Range: 0.0 – 22.0 ppm bromine mdl: 0.3 ppm method: in buff ered sample bromine reacts with diethyl-p-phenylene diamine (dpd) to produce a pink-red color in proportion to the concentration of ...

  • Page 111

    Test procedures procedure use 10 mm square cell adapter 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 11 bromine-udv ) from testing menu. 4. Scroll to and select 11 bromine-udv from menu. 5...

  • Page 112

    Test procedures bromine , udv smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 113: Cadmium

    Test procedures cadmium pan method • code 4017-01 quantity contents code 60 ml *buff ered ammonia reagent *4020-h 15 ml sodium citrate, 10% 6253-e 30 ml *pan indicator *4021-g 30 ml stabilizing reagent 4022-g 1 pipet, 1.0 ml, plastic 0354 2 pipet, 0.5 ml, plastic 0369 *warning: reagents marked with a...

  • Page 114

    Test procedures procedure 1. Use universal sample holder. 2. Press on button to turn spectrophotometer on. 3. Scroll to and select programmed tests. 4. Scroll to and select all tests (or another sequence containing 12 cadmium ) from testing menu. 5. Scroll to and select 12 cadmium from menu. 6. Rins...

  • Page 115: Chloride, Testab

    Test procedures chloride, testab argentometric testab method • code 3693-sc quantity contents code 50 *chloride spectrophotometric grade tablets *3885a-h 1 tablet crusher 0175 *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data sheet (sds...

  • Page 116

    Test procedures procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 21 chloride-testab ) from testing menu. 4. Scroll to and select 21 chloride-testab from m...

  • Page 117: Chlorine

    Test procedures chlorine liquid dpd method • code 4859 quantity contents code 30 ml dpd 1a free chlorine reagent p-6740-g 30 ml *dpd 1b free chlorine reagent *p-6741-g 30 ml dpd 3 total chlorine reagent p-6743-g *warning: reagents marked with an * are considered to be potential health hazards. To vi...

  • Page 118

    Test procedures application: drinking, surface, and saline waters; swimming pool water; domestic and industrial wastes. Range: 0.00–4.00 ppm chlorine mdl: 0.025 ppm method: in the absence of iodide, free available chlorine reacts instantly with dpd to produce a red color. Subsequent addition of pota...

  • Page 119

    Test procedures procedure–free chlorine use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 17 cl liquid dpd ) from testing menu. 4. Scroll to and select 17 cl liquid ...

  • Page 120

    Test procedures chlorine, liquid smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 121: Chlorine

    Test procedures chlorine dpd tablet method • code 3643-sc quantity contents code 100 dpd #1 instrument grade tablets 6903a-j 100 dpd #3 instrument grade tablets 6197a-j 15 ml glycine solution 6811-e *warning: reagents marked with an * are considered to be potential health hazards. To view or print a...

  • Page 122

    Test procedures application: drinking, surface, and saline waters; swimming pool water; domestic and industrial wastes. Range: 0.00–4.00 ppm chlorine mdl: 0.02 ppm method: in the absence of iodide, free available chlorine reacts instantly with dpd to produce a red color. Subsequent addition of potas...

  • Page 123

    Test procedures procedure–free chlorine use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 15 chlorine ) from testing menu. 4. Scroll to and select 15 chlorine from ...

  • Page 124

    Test procedures chlorine, dpd tablet smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 125: Chlorine-Free, Udv

    Test procedures chlorine-free, udv dpd unit dose vial method • code 4311-j quantity contents code 1 free chlorine unit dose vials, 20 pouches 4311-j equipment needed but not supplied: standard accessory package • code 1961 1 package of 3 vials (empty) 0156 1 syringe, 3 ml, plastic 1184 1 foil storag...

  • Page 126

    Test procedures the fact that chlorine can be easily detected and measured makes chlorine a favorite water sanitizer of those concerned with the public safety of water supplies. Chlorine concentrations in the range of 0.1 to 0.4 parts per million are usually maintained in municipal supplies. Chlorin...

  • Page 127

    Test procedures procedure use 10 mm square cell adapter 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 16 cl free-udv ) from testing menu. 4. Scroll to and select 16 cl free-udv from menu. 5...

  • Page 128

    Test procedures chlorine-free , udv smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 129: Chlorine–Total, Udv

    Test procedures chlorine–total, udv dpd unit dose vial method • code 4312-j quantity contents code 1 total chlorine unit dose vials, 20 pouches 4312-j equipment needed but not supplied: standard accessory package • code 1961 1 package of 3 vials (empty) 0156 1 syringe, 3 ml, plastic 1184 1 foil stor...

  • Page 130

    Test procedures the fact that chlorine can be easily detected and measured makes chlorine a favorite water sanitizer of those concerned with the public safety of water supplies. Chlorine concentrations in the range of 0.1 to 0.4 parts per million are usually maintained in municipal supplies. Chlorin...

  • Page 131

    Test procedures procedure use 10 mm square cell adapter 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 18 cl total-udv ) from testing menu. 4. Scroll to and select 18 cl total-udv from menu....

  • Page 132

    Test procedures chlorine-total , udv smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 133: Chlorine Dioxide

    Test procedures chlorine dioxide dpd method • code 3644-sc quantity contents code 100 dpd #1 instrument grade tablets 6903a-j 15 ml glycine solution 6811-e *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data sheet (sds) for these reagents...

  • Page 134

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests from menu. 3. Scroll to and select all tests (or another sequence containing 20 chlor diox ) from testing menu. 4. Scroll to and select 20 chlor diox from ...

  • Page 135: Chromium, Hexavalent

    Test procedures chromium, hexavalent diphenylcarbohydrazide method • code 3645-sc quantity contents code 10 g *chromium reagent powder *v-6276-d 1 spoon, 0.1 g, plastic 0699 50 filter paper 0465-h 1 funnel, plastic 0459 *warning: reagents marked with an * are considered to be potential health hazard...

  • Page 136

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests from menu. 3. Scroll to and select all tests (or another sequence containing 22 chromium ) from testing menu. 4. Scroll to and select 22 chromium from menu...

  • Page 137

    Test procedures chromium-hexavalent, trivalent & total diphenylcarbohydrazide method • code 3698-sc quantity contents code 60 ml *sulfuric acid, 5n *7681-h 10 g *chromium reagent powder *v-6276-d 15 ml *sodium azide, 5% *7683-e 30 ml potassium permanganate, 0.5% 7682-g 60 ml deionized water 5115pt-h...

  • Page 138

    Test procedures application: drinking, surface, and saline waters; domestic and industrial wastes. Range: 0.00–1.00 ppm chromium mdl: 0.01 ppm method: the trivalent chromium is converted to hexavalent chromium by permanganate under acidic conditions. Hexavalent chromium reacts with 1,5 diphenyl- car...

  • Page 139

    Test procedures hexavalent chromium procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select 22 chromium from menu. 3. Scroll to and select all tests (or another sequence containing 22 chromium ) from testing menu. 4. Scroll to and select 22 chr...

  • Page 140

    Test procedures with deionized water (5115). 9. Press on button to turn spectrophotometer on. 10. Scroll to and select programmed tests from menu. 11. Scroll to and select all tests (or another sequence containing 22 chromium ) from testing menu. 12. Scroll to and select 22 chromium from menu. 13. R...

  • Page 141: Chromium, Testab

    Test procedures chromium, testab diphenylcarbohydrazide testab method • code 3697-sc quantity contents code 50 *chromium spectrophotometric grade tablets *3889a-h 1 tablet crusher 0175 *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data s...

  • Page 142

    Test procedures procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 23 chromium-testab ) from testing menu. 4. Scroll to and select 23 chromium-testab from m...

  • Page 143: Cobalt

    Test procedures cobalt pan method • code 4851 quantity contents code 60 ml *cobalt buff er *4852-h 60 ml *cobalt indicator reagent *4853-h 30 ml *stabilizer solution *4854-g 2 pipet, 1.0 ml, plastic 0354 1 pipet, 0.5 ml, plastic 0353 *warning: reagents marked with an * are considered to be potential ...

  • Page 144

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 24 cobalt ) from testing menu. 4. Scroll to and select 24 cobalt from menu. 5. Rinse a t...

  • Page 145: Cod, Low Range

    Test procedures cod, low range mercury free digestion method • code 0072-sc mercury digestion method • code 0075-sc quantity contents code 25 *cod low level mercury free tubes *0072-sc or 25 *cod low level mercury tubes *0075-sc *warning: reagents marked with an * are considered to be potential heal...

  • Page 146

    Test procedures application: domestic and industrial wastes. Range: 0–150 mg/l cod mdl: 5 mg/l method: dichromate in the presence of silver salts, at high temperature in a closed system, oxidizes most organic compounds to 95-100% of the theoretical amount. This process is called digestion. As dichro...

  • Page 147

    Test procedures procedure use universal sample holder 1. Homogenize sample if necessary. 2. Preheat cod heater block to 150±2°c. 3. Remove cap from cod tube. Hold tube at a 45° angle. Use a volumetric pipet, to carefully add 2.0 ml sample water allowing the sample to run down the side of the tube. 4...

  • Page 148

    Test procedures the reacted blank will be stable if stored in the dark. To eliminate error caused by contamination, wash all glassware with 20% sulfuric acid. For greater accuracy, a minimum of three repetitions should be performed and the results averaged. Some samples may be digested completely in...

  • Page 149: Cod, Standard Range

    Test procedures cod, standard range mercury free digestion method • code 0073-sc mercury digestion method • code 0076-sc quantity contents code 25 *cod standard level mercury free tubes *0073-sc or 25 *cod standard level mercury tubes *0076-sc *warning: reagents marked with an * are considered to be...

  • Page 150

    Test procedures application: domestic and industrial wastes. Range: 0–1500 mg/l cod mdl: 50 mg/l method: dichromate in the presence of silver salts, at high temperature in a closed system, oxidizes most organic compounds to 95-100% of the theoretical amount. This process is called digestion. As dich...

  • Page 151

    Test procedures procedure use universal sample holder 1. Homogenize sample if necessary. 2. Preheat cod heater block to 150±2°c. 3. Remove cap from cod tube. Hold tube at a 45° angle. Use a volumetric pipet, to carefully add 2.0 ml sample water allowing the sample to run down the side of the tube. 4...

  • Page 152

    Test procedures the reacted blank will be stable if stored in the dark. To eliminate error caused by contamination, wash all glassware with 20% sulfuric acid. For greater accuracy, a minimum of three repetitions should be performed and the results averaged. Some samples may be digested completely in...

  • Page 153: Cod, High Range

    Test procedures cod, high range mercury free digestion method • code 0074-sc mercury digestion method • code 0077-sc quantity contents code 25 *cod high level mercury free tubes *0074-sc or 25 *cod high level mercury tubes *0077-sc *warning: reagents marked with an * are considered to be potential h...

  • Page 154

    Test procedures application: domestic and industrial wastes. Range: 0–15,000 mg/l cod mdl: 500 mg/l method: dichromate in the presence of silver salts, at high temperature in a closed system, oxidizes most organic compounds to 95-100% of the theoretical amount. This process is called digestion. As d...

  • Page 155

    Test procedures procedure use universal sample holder 1. Homogenize sample if necessary. 2. Preheat cod heater block to 150±2°c. 3. Remove cap from cod tube. Hold vial at a 45° angle. Use a graduated pipet, to carefully add 0.2 ml sample water allowing the sample to run down the side of the tube. 4....

  • Page 156

    Test procedures to eliminate error caused by contamination, wash all glassware with 20% sulfuric acid. For greater accuracy, a minimum of three repetitions should be performed and the results averaged. Some samples may be digested completely in less than two hours. The concentration may be measured ...

  • Page 157: Color

    Test procedures color platinum cobalt method • no reagents required color in water may be attributed to humus, peat, plankton, vegetation, and natural metallic ions, such as iron and manganese, or industrial waste. Color is removed to make water suitable for domestic and industrial use. Color may ha...

  • Page 158

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 28 color ) from testing menu. 4. Scroll to and select 28 color from menu. 5. Rinse a tub...

  • Page 159: Copper, Low Range

    Test procedures copper, low range bicinchoninic acid method • code 3640-sc quantity contents code 50 *copper tablets *3808a-h *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data sheet (sds) for these reagents go to www.Lamotte.Com. Search...

  • Page 160

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed test from menu. 3. Scroll to and select all tests (or another sequence containing 29 copper bca-lr ) from testing menu. 4. Scroll to and select 29 copper bca-lr ...

  • Page 161: Copper

    Test procedures copper cuprizone method • code 4023 quantity contents code 15 ml *copper a *p-6367-e 15 ml *copper b *p-6368-e *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data sheet (sds) for these reagents go to www.Lamotte.Com. Searc...

  • Page 162

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 31 cu-cuprizone ) from testing menu. 4. Scroll to and select 31 cu-cuprizone from menu. ...

  • Page 163: Copper, Ddc

    Test procedures copper, ddc diethyldithiocarbamate method • code 3646-sc quantity contents code 15 ml *copper 1 *6446-e *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data sheet (sds) for these reagents go to www.Lamotte.Com. Search for t...

  • Page 164

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests from menu. 3. Scroll to and select all tests (or another sequence containing 32 copper ddc ) from testing menu. 4. Scroll to and select 32 copper ddc from ...

  • Page 165: Copper, Udv

    Test procedures copper, udv bicinchoninic acid–unit dose vial method • code 4314-j quantity contents code 1 *copper unit dose vials, 20 pouches *4314-j equipment needed but not suppled: standard accessory package • code 1961 1 package of 3 vials (empty) 0156 1 syringe, 3 ml, plastic 1184 1 foil stor...

  • Page 166

    Test procedures application: drinking, surface, and saline waters; domestic and industrial wastes. Range: 0.00–4.00 copper mdl: 0.20 ppm method: cupric ions form a purple complex with bicinchoninic acid around ph 6–7, in proportion to the concentration of copper in the sample. Sample handling & pres...

  • Page 167

    Test procedures procedure use 10 mm square cell adapter. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 33 copper-udv ) from testing menu. 4. Scroll to and select 33 copper-udv from menu. 5....

  • Page 168

    Test procedures copper , udv smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 169: Cyanide

    Test procedures cyanide pyridine-barbituric acid method • code 3660-sc quantity contents code 60 ml cyanide buff er 2850ps-h 5 g *cyanide cl reagent *2794ds-c 5 g cyanide indicator reagent 2793ds-c 15 ml *hydrochloric acid 1n *6130-e 15 ml *sodium hydroxide 1n *4004-e 2 spoons, 0.1 g, plastic 0699 1 ...

  • Page 170

    Test procedures application: low level concentrations in drinking and surface waters; domestic and industrial waters. This method determines only those cyanides amenable to chlorination. Range: 0.00–0.50 ppm cyanide mdl: 0.05 ppm method: cyanides react with a chlorine donor to form cyanogen chloride...

  • Page 171

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests from menu. 3. Scroll to and select all tests (or another sequence containing 35 cyanide ) from testing menu. 4. Scroll to and select 35 cyanide from menu. ...

  • Page 172

    Test procedures cyanide smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 173: Cyanuric Acid

    Test procedures cyanuric acid melamine method–turbidity • code 366i-01-sc quantity contents code 2 x 100 ml cyanuric acid test solution 4856-j 1 syringe, 5 ml 0807 *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data sheet (sds) for these ...

  • Page 174

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 36 cyanuric ) from testing menu. 4. Scroll to and select 36 cyanuric from menu. 5. Rinse...

  • Page 175: Cyanuric Acid, Udv

    Test procedures cyanuric acid, udv melamine–turbidity–unit dose vial method • code 4313-j quantity contents code 1 *cyanuric acid unit dose vials, 20 pouches *4313-j equipment but not suppled: standard accessory package • code 1961 1 package of 3 vials (empty) 0156 1 syringe, 3 ml, plastic 1184 1 fo...

  • Page 176

    Test procedures application: swimming pool water. Range: 0–150 cyanuric acid mdl: 5 ppm method: a buff ered solution of melamine forms a precipitate with cyanuric acid in proportion to the amount of cyanuric acid present. The amount of particles in suspension is measured turbidimetrically. Sample han...

  • Page 177

    Test procedures procedure use 10 mm square cell adapter. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 37 cyanuric-udv ) from testing menu. 4. Scroll to and select 37 cyanuric-udv from menu...

  • Page 178

    Test procedures cyanuric acid , udv smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 179: Dissolved Oxygen

    Test procedures dissolved oxygen winkler colorimetric method • code 3688-sc quantity contents code 30 ml *manganese sulfate solution *4167-g 30 ml *alkaline potassium iodide azide *7166-g 30 ml *sulfuric acid 1:1 *6141wt-g 1 sample tube, screw cap 29180 1 cap 28570 *warning: reagents marked with an ...

  • Page 180

    Test procedures collection & treatment of the water sample steps 1 through 4 below describe proper sampling technique in shallow water. For sample collection at depths beyond arm’s reach, special water sampling apparatus is required (e.G. The lamotte water sampling chamber, code 1060; model jt-1 wat...

  • Page 181

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 39 do ) from testing menu. 4. Scroll to and select 39 do from menu. 5. Rinse a clean tub...

  • Page 182

    Test procedures dissolved oxygen smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 183: Fluoride

    Test procedures fluoride spadns method • code 3647-02-sc quantity contents code 4 x 30 ml *acid-zirconyl-spadns reagent *3875-g 2 x 30 ml *sodium arsenite solution *4128-g 1 pipet, 0.5 ml, plastic 0353 1 pipet, 1.0 ml, plastic 0354 *warning: reagents marked with an * are considered to be potential h...

  • Page 184

    Test procedures application drinking and surface waters; domestic and industrial waters. Range: 0.0–2.0 ppm fluoride mdl: 0.1 ppm method: colorimetric test based upon the reaction between fl uoride and zirconium dye lake. The fl uoride reacts with the dye lake, dissociating a portion of it into a colo...

  • Page 185

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 41 fluoride ) from testing menu. 4. Scroll to and select 41 fluoride from menu 5. This t...

  • Page 186

    Test procedures fluoride smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 187: Hardness, Total-Udv

    Test procedures hardness, total-udv unit dose vial method • code 4309-j quantity contents code 1 *calcium hardness unit dose vials, 20 pouches *4309-j equipment needed but not supplied: standard accessory package • code 1961 1 package of 3 vials (empty) 0156 1 syringe, 3 ml, plastic 1184 1 foil stor...

  • Page 188

    Test procedures application: drinking and surface waters; swimming pool water. Range: 10–500 as caco 3 total hardness mdl: 10 ppm method: calcium and magnesium react in a strongly buff ered medium with an indicator to develop a pale purple color in proportion to the concentration. Sample handling & p...

  • Page 189

    Test procedures procedure use 10 mm square cell adapter 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 13 ca&mg hard-udv ) from testing menu. 4. Scroll to and select 13 ca&mg hard-udv from m...

  • Page 190

    Test procedures hardness , total-udv smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 191: Hydrazine

    Test procedures hydrazine p-dimethylaminobenzaldehyde method • code 3656-01-sc quantity contents code 2x60 ml *hydrazine reagent a *4841-h 10 g hydrazine reagent b powder 4842-d 1 pipet, 1.0 ml, plastic 0354 1 spoon, 0.15 g, plastic 0727 *warning: reagents marked with an * are considered to be poten...

  • Page 192

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 45 hydrazine ) from testing menu. 4. Scroll to and select 45 hydrazine from menu. 5. Rin...

  • Page 193: Hydrogen Peroxide, Low Range

    Test procedures hydrogen peroxide, low range dpd method • code 3662-sc quantity contents code 30 ml hydrogen peroxide reagent #1 6452-g 100 *hydrogen peroxide lr tablets *6454a-j 1 tablet crusher 0175 *warning: reagents marked with an * are considered to be potential health hazards. To view or print...

  • Page 194

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 46 h peroxide-lr ) from testing menu. 4. Scroll to and select 46 h peroxide-lr from menu...

  • Page 195

    Test procedures hydrogen peroxide, high range dpd method • code 4045-01 quantity contents code 30 ml *hydrogen peroxide reagent #1 *6452-g 100 *hydrogen peroxide lr tablets *6454a-j 1 tablet crusher 0175 1 pipet, glass 0342 *warning: reagents marked with an * are considered to be potential health ha...

  • Page 196

    Test procedures application: drinking, industrial, domestic and swimming pool waters range: 0–60 ppm hydrogen peroxide mdl: 1 ppm method: hydrogen peroxide reacts with an excess of potassium iodide through the action of a catalyst and buff er to release an equivalent amount of iodine. The iodine in t...

  • Page 197

    Test procedures procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Select all tests (or another sequence containing 47 h peroxide-hr) from testing menu . 4. Scroll to and select 47 h peroxide-hr from menu. 5. Use the pi...

  • Page 198

    Test procedures hydrogen peroxide , high range smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 199: Hydrogen Peroxide, Shock

    Test procedures hydrogen peroxide, shock dpd method • code 4045 quantity contents code 30 ml hydrogen peroxide reagent #1 6452-g 100 *hydrogen peroxide lr tablets *6454a-j 1 tablet crusher 0175 1 pipet, glass 0342 *warning: reagents marked with an * are considered to be potential health hazards. To ...

  • Page 200

    Test procedures procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Select all tests (or another sequence containing 48 h peroxide-shock) from testing menu . 4. Scroll to and select 48 h peroxide-shock from menu. 5. Use ...

  • Page 201: Iodine

    Test procedures iodine dpd tablet method • code 3643-sc quantity contents code 100 dpd #1 instrument grade tablets 6903a-j 100 dpd #3 instrument grade tablets 6197a-j 15 ml glycine solution 6811-e *warning: reagents marked with an * are considered to be potential health hazards. To view or print a s...

  • Page 202

    Test procedures like chlorine and bromine, iodine is an eff ective germicidal agent employed in drinking water treatment, pool and spa water sanitation, food service sanitation, and other public health applications. Application: drinking, surface, and saline waters; swimming pool water; domestic and ...

  • Page 203

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 50 iodine ) from testing menu. 4. Scroll to and select 50 iodine from menu. 5. Rinse a c...

  • Page 204

    Test procedures iodine , dpd tablet smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 205: Iron

    Test procedures iron bipyridyl method • code 3648-sc quantity contents code 30 ml *iron reagent #1 *v-4450-g 5 g *iron reagent #2 powder *v-4451-c 1 pipet, 0.5 ml, plastic 0353 1 spoon, 0.1 g, plastic 0699 *warning: reagents marked with an * are considered to be potential health hazards. To view or ...

  • Page 206

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 51 iron bipyr ) from testing menu. 4. Scroll to and select 51 iron bipyr from menu. 5. R...

  • Page 207: Iron

    Test procedures iron i,i0-phenanthroline method • code 3668-sc quantity contents code 15 ml *acid phenanthroline indicator *2776-e 5 g *iron reducing reagent *2777-c 1 spoon, 0.1 g, plastic 0699 *warning: reagents marked with an * are considered to be potential health hazards. To view or print a saf...

  • Page 208

    Test procedures application: drinking, surface, and saline waters; domestic and industrial wastes. Range: 0.00–4.50 ppm iron mdl: 0.04 ppm method: ferric iron is reduced to ferrous iron and subsequently forms a colored complex with phenanthroline for a quantitative measure of total iron. Sample hand...

  • Page 209

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 53 iron phen ) from testing menu. 4. Scroll to and select 53 iron phen from menu. 5. Rin...

  • Page 210

    Test procedures iron , i,i0-phenanthroline smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 211: Iron, Udv

    Test procedures iron, udv bipyridyl–unit dose vial method • code 4315-j quantity contents code 1 *total iron unit dose vials, 20 pouches *4315-j *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data sheet (sds) for these reagents go to www....

  • Page 212

    Test procedures application: drinking, surface, and saline waters; domestic and industrial wastes. Range: 0.00–10.00 ppm iron mdl: 0.07 ppm method: ferric iron is reduced to ferrous iron and subsequently forms a colored complex with for a quantitative measure of total iron. Sample handling & preserv...

  • Page 213

    Test procedures procedure use 10 mm square cell adapter. 1. Press on button to turn spectrophotometer on.. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 52 iron-udv ) from testing menu. 4. Scroll to and select 52 iron-udv from menu. 5. Ri...

  • Page 214

    Test procedures iron , udv smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 215: Lead

    Test procedures lead par method • code 4031-01 quantity contents code 250 ml *ammonium chloride buff er *4032-k 15 ml *sodium cyanide, 10% *6565-e 30 ml par indicator 4033-g 30 ml stabilizing reagent 4022-g 15 ml *ddc reagent *4034-e 1 syringe, 5 ml, plastic 0807 2 pipet, 0.5 ml, plastic 0353 *warnin...

  • Page 216

    Test procedures application: drinking and surface waters; domestic and industrial wastewaters. Range: 0.00–5.00 lead mdl: 0.10 ppm method: lead and calcium ions form a red complex with par (4-(2’-pyridylazo)resorcinol), at a ph of about 10. When sodium diethyldithiocarbamate is added, the lead/par c...

  • Page 217

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 54 lead ) from testing menu. 4. Scroll to and select 54 lead from menu. 5. Rinse a tube ...

  • Page 218

    Test procedures lead smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 219: Manganese, Low Range

    Test procedures manganese, low range pan method • code 3658-01-sc quantity contents code 2 x 60 ml *hardness buff er reagent *4255-h 30 ml *manganese indicator reagent *3956-g 15 ml *sodium cyanide, 10% *6565-e 1 pipet, 0.5 ml, plastic 0369 1 pipet, 1.0 ml, plastic 0354 *warning: reagents marked with...

  • Page 220

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 55 manganese l ) from testing menu. 4. Scroll to and select 55 manganese l from menu. 5....

  • Page 221: Manganese, High Range

    Test procedures manganese, high range periodate method • code 3669-sc quantity contents code 10 g manganese buff er reagent 6310-d 15 g * manganese periodate reagent *6311-e 1 spoon, 0.1 g, plastic 0699 1 spoon, 0.15 g, plastic 0727 *warning: reagents marked with an * are considered to be potential h...

  • Page 222

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 56 manganese h ) from testing menu. 4. Scroll to and select 56 manganese h from menu. 5....

  • Page 223: Mercury

    Test procedures mercury tmk method • code 4861 quantity contents code 50 *tmk tablets *4862-h 2 x 250 ml *propyl alcohol *4863-k 250 ml *acetate buff er *4864-k 1 tablet crusher 0175 1 test tube, 10 ml, glass, w/cap 0778 1 pipet, 1.0 ml, plastic 0354 1 pipet, 0.5 ml, plastic 0353 *warning: reagents m...

  • Page 224

    Test procedures preparation of *tmk indicator note: prepare *tmk indicator daily. Keep out of direct sunlight. 1. Fill test tube (0778) to the 10 ml line with *propyl alcohol (4863). 2. Add one *tmk tablet (4862). 3. Use tablet crusher (0175) to completely crush tablet. 4. Cap and mix. Shake vigorou...

  • Page 225: Molybdenum

    Test procedures molybdenum thioglycolate method • code 3699-03-sc quantity contents code 2 x 30 ml * mo buff er *3997-g 2 x 30 ml * molybdenum oxidizing reagent *6485-g 2.5g * molybdenum indicator powder *6486-s 1 spoon, 0.05g, plastic 0696 2 pipets, 1.0 ml, plastic w/cap 0372 *warning: reagents mark...

  • Page 226

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 61 molybdenum-hr ) from testing menu. 4. Scroll to and select 61 molybdenum-hr from menu...

  • Page 227: Nickel

    Test procedures nickel dimethylglyoxime method • code 3663-01-sc quantity contents code 60 ml * hydrochloric acid, 2.5n *6251ps-h 30 g * ammonium persulfate reagent *6566-g 30 ml * silver nitrate solution, 0.0141n *6346wt-g 250 ml sodium citrate, 10% 6253-k 60 ml * dimethylglyoxime, 1% *6254-h 60 ml...

  • Page 228

    Test procedures procedure use universal sample holder. 1. Use the 10 ml graduated cylinder (0416) to measure 10 ml of sample water. Pour into glass test tube (0608). 2. Use the 1 ml pipet (0354) to add 1 ml of *hydrochloric acid, 2.5n (6251). 3. Use the 0.1 g spoon (0699) to add 2 measures of *ammon...

  • Page 229: Nitrate

    Test procedures nitrate zinc reduction • code 3689-sc quantity contents code 50 *nitrate spectrophotometic grade tablets *3881a-h 1 tablet crusher 0175 *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data sheet (sds) for these reagents go ...

  • Page 230

    Test procedures application: drinking, surface, and saline waters; domestic and industrial waters. Range: 0–60 ppm nitrate mdl: 2.5 ppm method: zinc is used to reduce nitrate to nitrite. The nitrite that was originally present, plus the reduced nitrate, reacts with chromotropic acid to form a red co...

  • Page 231

    Test procedures procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 66 nitrate-testab ) from testing menu. 4. Scroll to and select 66 nitrate-testab from men...

  • Page 232

    Test procedures nitrate , tablet smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 233: Nitrate-Nitrogen, Low Range

    Test procedures nitrate-nitrogen, low range cadmium reduction method • code 3649-sc quantity contents code 2 x 60 ml * mixed acid reagent *v-6278-h 5 g * nitrate reducing reagent *v-6279-c 1 spoon, 0.1 g, plastic 0699 1 dispenser cap 0692 *warning: reagents marked with an * are considered to be pote...

  • Page 234

    Test procedures application: drinking, surface, and saline waters; domestic and industrial waters. Range: 0.00–3.00 ppm nitrate nitrogen mdl: 0.05 ppm method: powdered cadmium is used to reduce nitrate to nitrite. The nitrite that is originally present plus reduced nitrate is determined by diazotiza...

  • Page 235

    Test procedures procedure use universal sample holder. Note: place dispenser cap (0692) on *mixed acid reagent (v-6278). Save this cap for refi ll reagents. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequenc...

  • Page 236

    Test procedures nitrate-nitrogen , low range smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 237: Nitrite-Nitrogen, Low Range

    Test procedures nitrite-nitrogen, low range diazotization method • code 3650-sc quantity contents code 2 x 60 ml * mixed acid reagent *v-6278-h 5 g * color developing reagent *v-6281-c 1 spoon, 0.1 g, plastic 0699 1 dispenser cap 0692 *warning: reagents marked with an * are considered to be potentia...

  • Page 238

    Test procedures procedure use universal sample holder. Note: place dispenser cap (0692) on *mixed acid reagent (v-6278). Save this cap for refi ll reagents. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequenc...

  • Page 239: Nitrite

    Test procedures nitrite zinc reduction testab method • code 3694-sc quantity contents code 50 *nitrite spectrophotometric grade tablets *3886a-h 1 tablet crusher 0175 *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data sheet (sds) for the...

  • Page 240

    Test procedures procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 69 nitrite-testab ) from testing menu. 4. Scroll to and select 69 nitrite-testab from men...

  • Page 241: Nitrogen, Total

    Test procedures nitrogen, total chromotropic acid with persulfate digestion method • code 4026-01 quantity contents code 25 total nitrogen hydroxide reagent tubes 4040-g 5 g *digestion reagent powder 4036-c 60 ml deionized water *5115ps-h 5 g *total nitrogen reagent a powder *4041-c 30 *total nitrog...

  • Page 242

    Test procedures nitrogen is essential for plant growth, but the presence of excessive amounts in water supplies presents a major pollution problem. Nitrogen compounds may enter water as nitrates or be converted to nitrates from agricultural fertilizers, sewage, industrial and packing house wastes, d...

  • Page 243

    Test procedures procedure use universal sample holder 1. Preheat cod reactor to 100 ±2°c. Follow safety precautions. 2. Remove caps from two *total nitrogen hydroxide reagent tubes (4040). 3. Use a 0.15 g spoon (0727) and a funnel (0459) to add one level measure of *digestion reagent powder (4036) t...

  • Page 244

    Test procedures inversion. 22. Wait 5 minutes. 23. Wipe the tubes with a damp towel to remove fi ngerprints and smudges. Wipe with a dry towel. 24. Insert the blank tube into the chamber. Select scan blank. Remove the blank tube from the spectrophotometer. 25. Insert the sample tube into the chamber....

  • Page 245: Oxygen Scavengers

    Test procedures oxygen scavengers test for deha (diethylhydroxylamine), carbohydrazide, erythorbic acid, hydroquinone, methylethylketoxime iron reduction method • code 4857-01 quantity contents code 15 ml *deha reagent #1 *4791-e 15 ml deha reagent #2 4792-e 15 ml *deha reagent #3 *4793-e *warning: ...

  • Page 246

    Test procedures application: boilers range: 0.000–0.700 ppm deha (diethylhydroxylamine) 0.000–0.900 ppm carbohydrazide 0.00–3.00 ppm erythorbic acid 0.00–1.80 ppm hydroquinone 0.00–3.00 ppm methylethylketoxime mdl: 0.005 ppm deha 0.005 ppm carbohydrazide 0.02 ppm erythorbic acid 0.01 ppm hydroquanin...

  • Page 247

    Test procedures deha procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 38 deha ) from testing menu. 4. Scroll to and select 38 deha from menu. 5. Rinse a t...

  • Page 248

    Test procedures carbohydrazide procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 14 carbohydrazide ) from testing menu. 4. Scroll to and select 14 carbohyd...

  • Page 249

    Test procedures erythorbic acid procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 40 erythorbic acid ) from testing menu. 4. Scroll to and select 40 erytho...

  • Page 250

    Test procedures hydroquinone procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 49 hydroquinone ) from testing menu. 4. Scroll to and select 49 hydroquinone...

  • Page 251

    Test procedures methylethylketoxime procedure use universal sample holder 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 58 m-e-ketoxime ) from testing menu. 4. Scroll to and select 58 m-e-k...

  • Page 252

    Test procedures oxygen scavengers smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 253: Ozone

    Test procedures ozone indigo method • code 365i-sc quantity contents code 15 ml chlorine inhibitor 3990-e 250 ml ozone buff er 3991-k 30 ml indigo blue stock solution 3989-g 1 sampling apparatus 0681 1 pipet, transfer, 1.0 ml 2-2170 1 pipet, transfer, 5 ml 0329 1 pump, 10 ml 30527 1 bottle, hr reagen...

  • Page 254

    Test procedures procedure–low range use universal sample holder. A. Preparation of hr reagent note: the quantity of indigo blue stock solution (3989) supplied will prepare one batch of hr reagent for the high range ozone procedure or fi ve batches of hr reagent for the low range ozone procedure. 1. U...

  • Page 255

    Test procedures note: hr reagent must be made fresh each week. If reagent is refrigerated, it may be kept up to 3 weeks. Procedure–high range use universal sample holder. A. Preparation of hr reagent note: the quantity of indigo blue stock solution (3989) supplied will prepare one batch of hr reagen...

  • Page 256

    Test procedures 14. Insert reacted sample tube into chamber, close lid and select scan sample. Record result. 15. Press and hold off button to turn spectrophotometer off or press exit button to exit to a previous menu or make another menu selection. Note: hr reagent must be made fresh each week. If r...

  • Page 257

    Test procedures ph colorimetric method • code 3700-01-sc quantity contents code 60 ml chlorphenol red indicator v-2209-h 60 ml phenol red indicator v-2304-h 60 ml thymol blue indicator v-2213-h 3 pipets, 0.5 ml, plastic w/caps 0369 *warning: reagents marked with an * are considered to be potential h...

  • Page 258

    Test procedures application: drinking, surface, and saline waters; swimming pool water; domestic and industrial wastes. Range: the various ph indicators exhibit a specifi c color change over a narrow ph range. The color changes are measured colorimetrically. Method: sample should be analyzed immediat...

  • Page 259

    Test procedures procedure use universal sample holder. 1. Use indicator, range, & test name chart to select the indicator, corresponding to anticipated ph range and to determine corresponding test name to select from spectrophotometer menu. 2. Press on button to turn spectrophotometer on. 3. Scroll ...

  • Page 260

    Test procedures ph smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 261: Phenol

    Test procedures phenol aminoantipyrine method • code 3652-sc quantity contents code 5 g aminoantipyrine reagent 7825-c 30 ml * ammonium hydroxide solution *7826-g 2 x 60 ml potassium ferricyanide solution 7827-h 1 spoon, 0.1 g, plastic 0699 1 pipet, plain, plastic 0352 1 pipet, 1.0 ml, plastic 0354 ...

  • Page 262

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 77 phenol ) from testing menu. 4. Scroll to and select 77 phenol from menu. 5. Rinse a c...

  • Page 263: Phosphate–Low Range

    Test procedures phosphate–low range ascorbic acid reduction method • code 3653-sc quantity contents code 60 ml * phosphate acid reagent *v-6282-h 5 g phosphate reducing reagent v-6283-c 1 pipet, 1 ml, plastic 0354 1 spoon, 0.1 g, plastic 0699 *warning: reagents marked with an * are considered to be ...

  • Page 264

    Test procedures application: drinking, surface, and saline waters; domestic and industrial wastes (method based on reactions that are specifi c for orthophosphate). Range: 0.00–3.00 ppm orthophosphate mdl: 0.04 ppm method: ammonium molybdate and antimony potassium tartrate react in a fi ltered acid me...

  • Page 265

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 78 phosphate l ) from testing menu. 4. Scroll to and select 78 phosphate l from menu. 5....

  • Page 266

    Test procedures phosphate , low range smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 267: Phosphate–High Range

    Test procedures phosphate–high range vanadomolybdophosphoric acid method • code 3655-sc quantity contents code 4 x 30 ml * vm phosphate reagent *4410-g 1 pipet, 1.0 ml, plastic 0354 *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data shee...

  • Page 268

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 79 phosphate h ) from testing menu. 4. Scroll to and select 79 phosphate h from menu. 5....

  • Page 269: Phosphorus-Total, Low Range

    Test procedures phosphorus-total, low range ascorbic acid reduction with persulfate digestion method code 4024-01 quantity contents code 25 *total phosphorus acid reagent tubes *4035-g 5 g *digestion reagent powder *4036-c 2 x 30 ml *total phosphorus lr hydroxide reagent *4038-g 2 x 30 ml *phosphate...

  • Page 270

    Test procedures phosphorus in natural waters and wastewaters occurs almost exclusively in the form of orthophosphates, condensed phosphates (pyro-, meta- and other polyphosphates) and organically bound phosphates. Phosphates may be added in small amounts to water supplies during treatment. Larger am...

  • Page 271

    Test procedures procedure use universal sample holder 1. Preheat cod reactor to 150 ±2°c. Follow safety precautions. 2. Remove cap from a *total phosphorus acid reagent tube (4035). Use a 1.0 ml pipet (0354) to add 5.0 ml of sample. 3. Use the 0.15 g spoon (0727) and a funnel (0459) to add one level...

  • Page 272

    Test procedures phosphorus , total, low range smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 273: Phosphorus-Total, High Range

    Test procedures phosphorus-total, high range molybdovanadate with acid persulfate digestion method code 4025-01 quantity contents code 25 *total phosphorus acid reagent tubes *4035-g 60 ml deionized water 5115ps-h 5 g *digestion reagent powder *4036- c 2 x 30 ml *total phosphorus hr hydroxide reagen...

  • Page 274

    Test procedures phosphorus in natural waters and wastewaters occurs almost exclusively in the form of orthophosphates, condensed phosphates (pyro-, meta- and other polyphosphates) and organically bound phosphates. Phosphates may be added in small amounts to water supplies during treatment. Larger am...

  • Page 275

    Test procedures procedure use universal sample holder 1. Preheat cod reactor to 150 ±2°c. Follow safety precautions. 2. Remove cap from a *total phosphorus acid reagent tube (4035). Use a 1.0 ml pipet (0354) to add 5.0 ml of deionized water (5115ps). This is the blank. 3. Remove cap from a *total ph...

  • Page 276

    Test procedures phosphorus , total, high range smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 277: Potassium

    Test procedures potassium tetraphenylboron method • code 3639-sc quantity contents code 30 ml * sodium hydroxide, 1.0n *4004wt-g 5 g * tetraphenylboron powder *6364-c 1 spoon, 0.05 g, plastic 0696 *warning: reagents marked with an * are considered to be potential health hazards. To view or print a s...

  • Page 278

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 81 potassium ) from testing menu. 4. Scroll to and select 81 potassium from menu. 5. Rin...

  • Page 279: Silica–Low Range

    Test procedures silica–low range heteropoly blue method • code 3664-sc quantity contents code 30 ml * silica reagent #1 *v-4466-g 30 ml * silica reagent #2 *v-4467-g 30 ml * silica reagent #3 *v-4468-g 10 g silica reagent #4 v-6284-d 1 spoon, 0.1 g, plastic 0699 *warning: reagents marked with an * a...

  • Page 280

    Test procedures application: drinking, surface, and saline waters; domestic and industrial wastes. Range: 0.00–2.50 ppm silica mdl: 0.03 ppm method: reactive silica forms a complex with ammonium molybdate in an acidic solution to produce a yellow-green color in proportion to the amount of silica pre...

  • Page 281

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 85 silica lo ) from testing menu. 4. Scroll to and select 85 silica lo from menu. 5. Rin...

  • Page 282

    Test procedures silica , low range smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 283: Silica–High Range

    Test procedures silica–high range silicomolybdate method • code 3687-sc quantity contents code 30 ml * silica reagent #1 *v-4466-g 30 ml * silica reagent #2 *v-4467-g 15 ml * silica reagent #3 *v-4468-g *warning: reagents marked with an * are considered to be potential health hazards. To view or pri...

  • Page 284

    Test procedures application: boiler and cooling waters; domestic and industrial wastes. Range: 0–50 ppm silica mdl: 1 ppm method: silica forms a complex with ammonium molybdate in an acidic solution to produce a yellow color in proportion to the amount of silica present. Phosphate also reacts with m...

  • Page 285

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 86 silica hi ) from testing menu. 4. Scroll to and select 86 silica hi from menu. 5. Rin...

  • Page 286

    Test procedures silica , high range smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 287: Sulfate

    Test procedures sulfate barium chloride method • code 3665-sc quantity contents code 10 g * sulfate reagent *v-6277-d 1 spoon, 0.1 g, plastic 0699 *warning: reagents marked with an * are considered to be potential health hazards. To view or print a safety data sheet (sds) for these reagents go to ww...

  • Page 288

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 89 sulfate-hr ) from testing menu. 4. Scroll to and select 89 sulfate-hr from menu. 5. R...

  • Page 289: Sulfide

    Test procedures sulfide methylene blue method • code 3654-02-sc quantity contents code 2 x 30 ml * sulfi de reagent a *v-4458-g 15 ml * sulfi de reagent b *v-4459-e 2 x 60 ml sulfi de reagent c 4460-h 2 pipets, 1.0 ml, plastic 0354 *warning: reagents marked with an * are considered to be potential heal...

  • Page 290

    Test procedures application: drinking, surface, and saline waters; domestic and industrial wastes. Range: 0.00–1.00 ppm sulfi de mdl: 0.02 ppm method: under suitable conditions the sulfi de ion reacts with p-aminodimethylaniline and ferric chloride to produce methylene blue in proportion to the sulfi d...

  • Page 291

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 90 sulfi de-lr ) from testing menu. 4. Scroll to and select 90 sulfi de-lr from menu. 5. R...

  • Page 292

    Test procedures sulfide smart spectro 2 test procedures 05.02.16 info@globalscientificsupply.Com www. Globalscientificsupply .Com global scientific supply the laboratory supply company!.

  • Page 293: Surfactants

    Test procedures surfactants ion pair extraction–bromphenol blue indicator method code 4876-01 quantity contents code 50 g ph adjustment powder 4509- h 10 g sodium chloride reagent 4877-d 2 x 60 ml *ds indicator reagent *4508-h 1 spoon, 0.5 g, plastic 0698 1 spoon, 0.1 g, plastic 0699 1 pipet, 1.0 ml...

  • Page 294

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 94 surfactants ) from testing menu. 4. Scroll to and select 94 surfactants from menu. 5....

  • Page 295: Tannin

    Test procedures tannin tungsto-molybdophosphoric acid method • code 3666-01-sc quantity contents code 30 ml * tannin reagent #1 *7833-g 2 x 60 ml * tannin reagent #2 *7834-h pipet, plain, plastic 0352 pipet, 1.0 ml, plastic 0354 *warning: reagents marked with an * are considered to be potential heal...

  • Page 296

    Test procedures procedure use universal sample holder. 1. Press on button to turn spectrophotometer on. 2. Scroll to and select programmed tests. 3. Scroll to and select all tests (or another sequence containing 96 tannin ) from testing menu. 4. Scroll to and select 96 tannin from menu. 5. Rinse a c...

  • Page 297: Turbidity

    Test procedures turbidity absorption method • no reagents required turbidity is a measure of water clarity and is independent of color. Turbidity is caused by undissolved and suspended solids. Mud, silt, algae, and microorganisms can all cause turbidity. Turbidity is a gross measurement of water qua...

  • Page 298

    Test procedures formazin stock solution the turbidity calibration curve for this instrument was prepared by using formazin solutions as a reference. A 4000 ftu standard solution is available (order code 6195- h, 60 ml) that can be diluted with low turbidity water to prepare solutions within the test...

  • Page 299: Zinc

    Test procedures zinc zincon method • code 3667-sc quantity contents code 30 ml * zinc indicator solution *6314-g 120 ml * methyl alcohol *6319-j 10 g sodium ascorbate powder 6316-d 25 g * zinc buff er powder *6315-g 15 ml * sodium cyanide, 10% *6565-e 30 ml * formaldehyde solution, 37% *5128-g 1 dilu...

  • Page 300

    Test procedures application: drinking and surface waters; domestic and industrial wastewaters. Range: 0.00–3.00 ppm zinc mdl: 0.025 ppm method: zinc forms a blue colored complex with zincon in a solution buff ered at ph 9.0. Other heavy metals are complexed by cyanide and the zinc cyanide complex is ...

  • Page 301

    Test procedures procedure use universal sample holder. A. Preparation of dilute zinc indicator solution 1. Use a pipet (0352) to measure exactly 5.0 ml of *zinc indicator solution (6314) into 10 ml graduated cylinder (0416). The bottom of the curved surface (the meniscus) of liquid should be at 5.0 ...

  • Page 302

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  • Page 303: Appendix

    Appendix ammonia in water occurs in two forms: toxic unionized ammonia (nh 3 ) and the relatively non-toxic ionized form, ammonium ion (nh 4+ ). This test method measures both forms as ammonia-nitrogen (nh 3 –n) to give the total ammonia-nitrogen concentration in water. The actual proportion of each...

  • Page 304

    For example: if a fresh water sample at 20°c has a ph of 8.5 and the test result is 1.0 ppm as total ammonia-nitrogen: 1. The percentage from the table is 11.18% (or 0.1118). 2. 1 ppm total ammonia-nitrogen x 0.1118 = 0.1118 ppm unionized ammonia- nitrogen. 3. Total ammonia-nitrogen 1.0000 ppm union...