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  5. Operating Manual And User Manual

Ocean Optics CHEM2000 Operating Manual And User Manual

Manual is about: Miniature Fiber Optic Spectrophotometers

Summary of CHEM2000

  • Page 1

    Operating manual and user’s guide chem2000 and chem2000-uv-vis miniature fiber optic spectrophotometers ocean optics, inc. 380 main street dunedin, fl 34698 (727) 733-2447 (727) 733-3962 fax for the latest information, consult our web site: www.Oceanoptics.Com or, e-mail our technical service depart...

  • Page 2

    Copyright © 2000 ocean optics, inc. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic, mechanical, photocopying, recording, or otherwise, without written permission from ocean optics, inc. This manual is sold as...

  • Page 3: Table of Contents

    Table of contents introduction ................................................................................................................................ 1 quick start .................................................................................................................................

  • Page 4

    Experiment tutorial .............................................................................................................. 27 absorbance experiments .................................................................................................... 27 transmission experiments .................

  • Page 5: Introduction

    Introduction ocean optics miniature fiber optic spectrometers and accessories have revolutionized the analytical instrumentation market by dramatically reducing the size and cost of optical sensing systems. More than 10,000 ocean optics spectrometers have been sold worldwide -- striking evidence of ...

  • Page 6

    2 pc2000 pc plug-in fiber optic spectrometer the pc2000 is 2048-element linear ccd-array fiber optic spectrometer mounted on a 1 mhz a/d card that fits easily into an isa-bus slot in the pc. The spectrometer has a 25- µ m slit and provides a resolution of 1.5 nm (fwhm). The 12-bit, 8-channel a/d car...

  • Page 7

    3 packing list a packing list comes with each order. It is located inside a plastic bag attached to the outside of the shipment box. The invoice is mailed separately. The items listed on your packing slip include all of the components in your order. However, some items on your packing list are actua...

  • Page 8: Quick Start

    4 quick start the chem2000 and chem2000-uv-vis systems are easy to set up, allowing the user to start collecting data within minutes. This quick start provides brief instructions on installing your pc2000, installing and configuring the software, and setting up your system. To find detailed directio...

  • Page 9: Step 5: Receive Data

    5 hardware configuration the configure hardware dialog box opens when you first run ooichem. The parameters in this dialog box are usually set only once -- when ooichem is first installed and the software first opens. 1. Under spectrometer type, choose s2000/pc2000. 2. Under a/d converter type, choo...

  • Page 10

    6 installing the pc2000 and ooichem software the pc2000 pc p lug - in s pectrometer has our 2048-element linear ccd-array fiber optic spectrometer mounted onto an adc1000 a/d card. The adc1000 a/d c ard is a high-speed isa-bus analog-to-digital converter card that connects our miniature fiber optic ...

  • Page 11

    7 for most computers, the default settings on our a/d converters work well. In the picture at left on page 6, it appears that the printer occupies irq setting 07, but for most computers, our a/d converters can share the irq 07 setting with a printer and conflicts will not arise. All computers have m...

  • Page 12

    8 install ooichem the resolution of your computer's monitor must be 800 x 600 or better to use ooichem software. Before installing ooichem, make sure that no other applications are running. 1. Insert “disk 1” into your floppy drive. (when prompted, install disks 2 and 3.) execute setup.Exe. 2. At th...

  • Page 13: Using Ooichem Software

    9 using ooichem software ooic hem o perating s oftware provides users with a real-time interface to a variety of acquisition, signal processing, display, and measurement functions. The 32-bit software program operates with windows 95/98 and windows nt and performs basic absorbance, emission and kine...

  • Page 14: Display Functions

    10 display functions several functions are accessed not through the menu but through buttons and task bars directly on the display screen, on the top and to the right of the graph area. (the resolution of your computer's monitor must be 800 x 600 or better to view ooichem software.) from the display...

  • Page 15

    11 relative irradiance selecting this mode switches the spectral window into relative irradiance mode. The reference spectrum must be made in scope mode with a blackbody of known color temperature. (chem2000- uv-vis users cannot make relative irradiance measurements because the light source that com...

  • Page 16

    12 cursor properties in this bar, you can label the cursor and monitor the cursor’s x value and y value. To the right of the x and y values of the cursor is a cursor selection button that allows you to choose a cursor style and a point style. You can also choose a color for the cursor and whether or...

  • Page 17

    13 subtract dark selecting this box subtracts the current dark spectrum from the spectra being displayed. This command is useful if you are trying to look at a change in an emission spectrum or are trying to eliminate from the spectra fixed pattern noise caused by a very long integration period. The...

  • Page 21

    17 trigger mode with the chem2000 or chem2000-uv-vis and ooichem operating software, you have two methods of acquiring data. In the no external trigger mode (or normal mode), the spectrometer is “free running.” that is, the spectrometer is continuously scanning, acquiring, and transferring data to y...

  • Page 23: Chem2000 Sampling Components

    19 chem2000 sampling components iss-2 integrated sampling system the chem2000 comes with a light source/sample holder combination called the iss-2 integrated sampling system. It is a fully integrated tungsten halogen light source and 1-cm square cuvette holder that couples to the pc2000 with optical...

  • Page 24

    20 using the filter slots in the iss-2 the filter slots (one at the light source, a second in the sample holder) can be used to hold filters or light blocks. The most useful filters include an fg-3 blue filter for increasing the relative energy near 400 nm and 800 nm compared to 600 nm, an ir cutoff...

  • Page 25

    21 iss-2 specifications path length: 1 cm collimating lens: bk 7 glass (~360 nm - 2 µ m*), 5 mm diameter, f/2 collimating lens termination: sma 905 filter slot: accepts filters up to ¼" (6 mm) in thickness base material: aluminum spectral range: 360 nm - 2 µ m* dimensions: 9.0 cm x 5.0 cm x 3.2 cm (...

  • Page 26: 400-

    22 400- µµµµ m diameter optical fiber the 400-µm diameter optical fiber that comes with your chem2000 is a single-strand optical fiber. The active part of the fiber consists of a silica core, surrounded by a silica cladding material. The fiber is very fragile, and if not protected by a suitable buff...

  • Page 27

    23 chem2000-uv-vis sampling components iss-uv-vis integrated sampling system the iss-uv-vis integrated sampling system that comes with the chem2000-uv-vis is a combination of a rf deuterium source with a tungsten halogen bulb connected to a cuvette holder for 1-cm cuvettes. The cuvette holder attach...

  • Page 28

    24 2. Plug the wall transformer into a standard 110 v outlet. Plug the 12 v output into the back of your iss-uv-vis above the 12v label. For users of european-version wall transformers, plug the transformer into a standard 220 v outlet. At this time, the green led indicator light on the front of the...

  • Page 29

    25 specifications deuterium wavelength range: 200-400 nm tungsten wavelength range: 360-850 nm deuterium bulb life: 800 hours tungsten bulb life: 2,000 hours ignition delay: ~1.5 seconds to 60 seconds peak-to-peak stability: 0.3% in 30 minutes 1.0% in 10 minutes connector: sma 905 power input: 12 v ...

  • Page 30: 300-

    26 300- µµµµ m diameter solarization-resistant optical fiber the 300-µm diameter solarization-resistant optical fiber that comes with your chem2000-uv-vis is a single-strand optical fiber. The active part of our solarization-resistant fibers consists of a silica core, surrounded by a silica cladding...

  • Page 31: Experiment Tutorial

    27 experiment tutorial when you are ready to begin your experiment, you should have already installed the pc2000, installed ooichem, set up your light source/sample holder, and connected your fiber from the pc2000 to your light source/sample holder. Now you are ready to take your measurements. Becau...

  • Page 32: Transmission Experiments

    28 transmission experiments transmission is the percentage of energy passing through a system relative to the amount that passes through the reference. Transmission mode is also used to show the portion of light reflected from a sample. Transmission and reflection measurements require the same mathe...

  • Page 33: Reflection Experiments

    29 reflection experiments reflection is the return of radiation by a surface, without a change in wavelength. The reflection may be: • specular, in which the angle of incidence is equal to the angle of reflection. • diffuse, in which the angle of incidence is not equal to the angle of reflection. Ev...

  • Page 34

    30 relative irradiance experiments irradiance is the amount of energy at each wavelength from a radiant sample. In relative terms, it is the fraction of energy from the sample compared to the energy collected from a lamp with a blackbody energy distribution, normalized to 1 at the energy maximum. Re...

  • Page 35: Concentration Experiments

    31 concentration experiments the absorbance of a solution is related to the concentration of the species within it. The relationship, known as beer’s law, is: a λ = ε λ c l where a is the absorbance at wavelength λ , ε is the extinction coefficient of the absorbing species at wavelength λ , c is the...

  • Page 38: Pc2000

    34 appendix a: changing the settings on the pc2000 base address settings to change the base address settings on the pc2000, find the bank of switches on the a/d converter. The base address may be changed via the first 6 switches (the irq may be changed via the last 3 switches). Switches in the off p...

  • Page 39

    35 interrupt request settings to change the irq settings on the pc2000, see the bank of switches on the a/d converter. The irq may be changed via the last 3 switches. The following matrix defines the different irq settings by switch positions 7, 8, and 9. In the default setting, the irq is set to 7....

  • Page 40

    36 appendix b: pc2000 pin-outs and jumpers it is not necessary for the average user to worry about the interconnect scheme, as the cables supplied with all of the units need only be plugged into the matching connectors on the hardware. However, if the need arises to design and fabricate your own cab...

  • Page 41: Your Spectrometer

    37 appendix c: calibrating the wavelength of your spectrometer the following describes how to calibrate the wavelength of your spectrometer. Though each spectrometer is calibrated before it leaves ocean optics, the wavelength for all spectrometers will drift slightly as a function of time and enviro...

  • Page 42

    38 independent dependent values computed from variable variables the regression output true wavelength (nm) pixel # pixel # 2 pixel # 3 predicted wavelength difference 253.65 296.73 302.15 313.16 334.15 365.02 404.66 407.78 435.84 546.07 576.96 579.07 696.54 706.72 727.29 738.40 751.47 175 296 312 3...