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  5. Operator's, Overview And Maintenance Manual

Waters Xevo G2 QTof Operator's, Overview And Maintenance Manual

Summary of Xevo G2 QTof

  • Page 1

    Waters xevo g2 qtof operator’s overview and maintenance guide revision b copyright © waters corporation 2010 2011 all rights reserved.

  • Page 2: Copyright Notice

    Ii copyright notice © 2010–2011 waters corporation. Printed in the united states of america and in ireland. All rights reserved. This document or parts thereof may not be reproduced in any form without the written permission of the publisher. The information in this document is subject to change wit...

  • Page 3: Customer Comments

    Iii customer comments waters’ technical communications department invites you to tell us of any errors you encounter in this document or to suggest ideas for otherwise improving it. Please help us better understand what you expect from our documentation so that we can continuously improve its accura...

  • Page 4: Safety Considerations

    Iv safety considerations some reagents and samples used with waters instruments and devices can pose chemical, biological, and radiological hazards. You must know the potentially hazardous effects of all substances you work with. Always follow good laboratory practice, and consult your organization’...

  • Page 5

    V glass-breakage hazard high-temperature hazard xevo g2 qtof high-temperature hazard: warning: to avoid injuries from broken glass, falling objects, or exposure to toxic substances, never place containers on top of the instrument or on its front covers. Warning: to avoid burn injuries, do not touch ...

  • Page 6: Safety Advisories

    Vi hazards associated with removing an instrument from service when you remove the instrument from use to repair or dispose of it, you must decontaminate all of its vacuum areas. These are the areas in which you can expect to encounter the highest levels of contamination: • source interior • waste t...

  • Page 7: Operating This Instrument

    Vii operating this instrument when operating this instrument, follow standard quality control procedures and the guidelines presented in this section. Applicable symbols audience and purpose this guide is for operators of varying levels of experience. It gives an overview of the instrument, and expl...

  • Page 8: Calibrating

    Viii calibrating to calibrate lc systems, follow acceptable calibration methods using at least five standards to generate a standard curve. The concentration range for standards should cover the entire range of qc samples, typical specimens, and atypical specimens. When calibrating mass spectrometer...

  • Page 9: Ec Authorized Representative

    Ix ec authorized representative waters corporation (micromass uk ltd.) floats road wythenshawe manchester m23 9lz united kingdom telephone: +44-161-946-2400 fax: +44-161-946-2480 contact: quality manager.

  • Page 10

    X.

  • Page 11: Table of Contents

    Table of contents xi copyright notice ................................................................................................... Ii trademarks ............................................................................................................ Ii customer comments .....................

  • Page 12

    Xii table of contents nanolockspray source and ionization modes .......................................... 1-9 combined appi/apci source ....................................................................... 1-11 intellistart fluidics system ............................................................

  • Page 13

    Table of contents xiii configuring for apci mode .......................................................................... 3-13 installing the apci probe ............................................................................. 3-13 removing the apci probe .........................................

  • Page 14

    Xiv table of contents removal and refitting of the source enclosure .......................................... 5-7 removing the source enclosure from the instrument .................................... 5-7 fitting the source enclosure to the instrument............................................ 5-1...

  • Page 15

    Table of contents xv cleaning the ion block assembly ................................................................. 5-46 disassembling the source ion block assembly.............................................. 5-46 cleaning the ion block components ..................................................

  • Page 16

    Xvi table of contents replacing the lockspray source’s assembly seals ................................. 5-98 removing the probe adjuster assembly probe and source enclosure seals ......................................................................................... 5-98 fitting the new source enc...

  • Page 17

    Table of contents xvii connecting to the collision cell gas supply ............................................. B-12 connecting the nitrogen exhaust line ...................................................... B-12 connecting the liquid waste line .......................................................

  • Page 18

    Xviii table of contents.

  • Page 19: Waters Xevo G2 Qtof Overview

    1-1 1 waters xevo g2 qtof overview this chapter describes the instrument, including its controls, sources and intellistart™ fluidics system. Contents: topic page waters xevo g2 qtof 1-2 lockspray source and ionization modes 1-6 nanolockspray source and ionization modes 1-9 combined appi/apci source ...

  • Page 20: Waters Xevo G2 Qtof

    1-2 waters xevo g2 qtof overview waters xevo g2 qtof the xevo™ g2 qtof mass spectrometry (ms) system is a hybrid, quadrupole, orthogonal acceleration, time-of-flight (oatof) mass spectrometer operated by waters ® informatics software. Either of the following high-performance, zspray™, dual-orthogona...

  • Page 21

    Waters xevo g2 qtof 1-3 acquity and nanoacquity xevo g2 qtof uplc/ms systems the waters xevo g2 qtof is compatible with the acquity uplc ® and nanoacquity uplc ® systems. If you are not using either system, refer to the documentation relevant to your lc system. The acquity ® xevo g2 qtof uplc ® /ms ...

  • Page 22

    1-4 waters xevo g2 qtof overview waters acquity xevo g2 qtof uplc/ms system: sample organizer (optional) solvent tray column heater xevo g2 qtof sample manager binary solvent manager access door to the fluidics pump high voltage connector for the esi probe probe source interface sliding door lockspr...

  • Page 23

    Waters xevo g2 qtof 1-5 nanoacquity uplc system the nanoacquity uplc system includes a binary solvent manager, auxiliary solvent manager, sample manager, column heater, sample organizer, detectors, and a specialized nanoacquity uplc column. Waters informatics software controls the system. For furthe...

  • Page 24: Software and Data System

    1-6 waters xevo g2 qtof overview software and data system waters informatics software controls the mass spectrometer. The software acquires, analyzes, manages, and distributes data from mass spectrometry, ultraviolet (uv), evaporative light scattering, and other sources. Waters informatics software ...

  • Page 25

    Lockspray source and ionization modes 1-7 xevo g2 qtof fitted with lockspray source: electrospray ionization (esi) in electrospray ionization (esi), a strong electrical charge is applied to the eluent as it emerges from a nebulizer. The droplets that compose the resultant aerosol undergo a reduction...

  • Page 26

    1-8 waters xevo g2 qtof overview the standard esi probe capillary accommodates flow rates of up to 2 ml/min making it suitable for lc applications in the range 100 µl/min to 2 ml/min. To reduce peak broadening for lower-flow-rate lc applications, such as 1-mm uplc columns, use the optional, small-bo...

  • Page 27: Ionization (Esci)

    Nanolockspray source and ionization modes 1-9 combined electrospray and atmospheric pressure chemical ionization (esci) in combined electrospray and atmospheric pressure chemical ionization (esci © ) mode, the standard esi probe is used in conjunction with a corona pin, to allow alternating acquisit...

  • Page 28

    1-10 waters xevo g2 qtof overview xevo g2 qtof fitted with nanolockspray source: the following options are available for the spraying capillary: • universal nanoflow™ nebulizer sprayer. This option, for flow injection or coupling to nanoacquity, uses a pump to regulate the flow rate as low as 100 nl...

  • Page 29: Combined Appi/apci Source

    Combined appi/apci source 1-11 combined appi/apci source atmospheric pressure photoionization (appi) uses photons generated by a discharge uv lamp (~10.2 ev) to produce sample ions from vaporized lc eluent. Direct photoionization of the sample molecule occurs when the photon energy exceeds the ioniz...

  • Page 30

    1-12 waters xevo g2 qtof overview intellistart fluidics system physical layout the intellistart fluidics system comprises the components shown in the following figure. Intellistart fluidics system components: a b c a a b b c a a b b c w aters w aters aa bb cc lock-spray selector valve sample selecto...

  • Page 31: System Operation

    Intellistart fluidics system 1-13 the intellistart fluidics system consists of these components: • a sample delivery system, with a pump, sample selector valve, and diverter valve used for lc and probe connections. • a lock-spray system, with a pump capable of ultra-low flow rates, a lock-spray sele...

  • Page 32: Ion Optics

    1-14 waters xevo g2 qtof overview ion optics the mass spectrometer’s ion optics operate as follows: 1. Samples from the lc or instrument’s solvent delivery system are introduced at atmospheric pressure into the ionization source. 2. The ions pass through the sample cone into the vacuum system. 3. Th...

  • Page 33: Leak Sensors

    Leak sensors 1-15 leak sensors leak sensors in the instrument’s drip trays continuously monitor for liquid leaks. A leak sensor stops system flow when its optical sensor detects about 1.5 ml of accumulated leaked liquid in its surrounding reservoir. At the same time, the instrument console displays ...

  • Page 34

    1-16 waters xevo g2 qtof overview.

  • Page 35: Preparing The Mass

    2-1 2 preparing the mass spectrometer for operation this chapter explains how to start up and shut down the mass spectrometer. Contents: topic page starting the mass spectrometer 2-2 preparing the intellistart fluidics system 2-4 rebooting the mass spectrometer 2-8 leaving the mass spectrometer read...

  • Page 36

    2-2 preparing the mass spectrometer for operation starting the mass spectrometer the waters xevo g2 qtof is compatible with the acquity uplc and nanoacquity uplc systems. If you are not using either system, refer to the documentation relevant to your lc system (see “software and data system” on page...

  • Page 37: Calibration

    Starting the mass spectrometer 2-3 6. Allow 4 minutes for the embedded pc to initialize. Tip: the power and status leds change as follows: • during initialization, the binary solvent manager’s and sample manager’s status led flashes green. • after the instruments are successfully powered-on, all pow...

  • Page 38

    2-4 preparing the mass spectrometer for operation flow rates for the xevo g2 qtof system the xevo g2 qtof system can run at high flow rates. To optimize desolvation, and thus sensitivity, run the system at appropriate gas flows and desolvation temperatures. Preparing the intellistart fluidics system...

  • Page 39

    Preparing the intellistart fluidics system 2-5 to install the reservoir bottles: 1. Remove the reservoir bottle caps. 2. Screw the reservoir bottles onto the mass spectrometer, as shown below. 3. For each reservoir bottle, ensure that the ends of the solvent delivery tubes are positioned so that the...

  • Page 40

    2-6 preparing the mass spectrometer for operation to install the low-volume vials : 1. If a standard reservoir bottle is fitted, remove it. 2. Screw the low-volume adaptors into the manifold and finger-tighten them. 3. Screw the low-volume vials into the adaptors. 4. For each low-volume vial, ensure...

  • Page 41

    Preparing the intellistart fluidics system 2-7 adjusting the solvent delivery tube positions for correct operation of the intellistart fluidics system, you must adjust each solvent delivery tube so that its end is close to, but does not, touch, the bottom of the reservoir bottle or low volume vial. ...

  • Page 42: Purging The Pump

    2-8 preparing the mass spectrometer for operation purging the pump whenever you replace a solution bottle, purge the pump with the solution that you are going to use next. See the mass spectrometer’s online help for details. Requirement: ensure that the end of the tubing is fully submerged in the so...

  • Page 43

    Emergency shutdown of the mass spectrometer 2-9 emergency shutdown of the mass spectrometer to shut down the mass spectrometer in an emergency: 1. Switch off the power at the electrical outlet. Result: the instrument turns off and vents. 2. Disconnect the power cable from the instrument’s rear panel...

  • Page 44

    2-10 preparing the mass spectrometer for operation.

  • Page 45: Configuring The Lockspray

    3-1 3 configuring the lockspray source this chapter explains how to configure the lockspray source for the following ionization modes: • esi (electrospray ionization) • apci (atmospheric pressure ionization) • esci (combined electrospray and atmospheric pressure ionization) contents: topic page conf...

  • Page 46: Configuring For Esi Mode

    3-2 configuring the lockspray source configuring the lockspray source the following table summarizes how you configure the lockspray source for the various ionization modes. Configuring for esi mode to operate in esi mode, you must fit the esi probe to the lockspray source enclosure. If you intend u...

  • Page 47

    Configuring for esi mode 3-3 to install the esi probe: 1. Prepare the instrument for working on the source (see page 5-7 ). 2. Remove the protective sleeve, if fitted, from the esi probe tip. 3. With the probe label facing you, carefully slide the esi probe into the hole in the probe adjuster assemb...

  • Page 48

    3-4 configuring the lockspray source esi probe, mounted on the lockspray source enclosure: 4. Tighten the probe locking ring to secure the probe in place. Tip: an automatic pressure test is performed when the probe is correctly seated in position. 5. Connect the esi probe’s cable to the high voltage...

  • Page 49

    Configuring for esi mode 3-5 recommendation: to reduce peak broadening, use 0.004-inch id tubing for sample flow rates 1.2 ml/min; use 0.005-inch id tubing for sample flow rates >1.2 ml/min. Requirements: • if you are replacing the tubing supplied with the instrument, you must minimize the length of...

  • Page 50: Removing The Esi Probe

    3-6 configuring the lockspray source removing the esi probe required materials chemical-resistant, powder-free gloves to remove the esi probe: 1. Prepare the instrument for working on the source (see page 5-7 ). 2. Disconnect the fluidics tubing from the esi probe. 3. Disconnect the esi probe’s cabl...

  • Page 51

    Installing the small-bore capillary option 3-7 installing the small-bore capillary option use the small-bore capillary option with 300-µm uplc columns in systems running at typical flow rates of 10 µl/min. The materials necessary to install the option are supplied in kit form. Required materials • c...

  • Page 52

    3-8 configuring the lockspray source to install the capillary: 1. Remove the existing capillary (see page 5-66 ). 2. Use the sharp knife or peek tubing cutter to cut an approximately 60-cm (24-inches) length of red, peek tubing. Requirement: cut the tubing squarely (that is, perpendicular to its hor...

  • Page 53

    Installing the small-bore capillary option 3-9 4. Using the needle-nose pliers, slide the unf coupler, ptfe liner sleeve, and a ferrule onto the capillary. 5. Insert the capillary in the peek union, and ensure that it is fully seated. 6. Screw the unf coupling into the peek union, finger-tight only....

  • Page 54

    3-10 configuring the lockspray source 12. Test for leaks by attaching the free end of the peek tubing to an lc pump and pumping mobile phase through it, at 200 µl/min. • if leakage occurs, disassemble and remake the connection, and then repeat the leak test. • if the back pressure on the lc pump is ...

  • Page 55

    Installing the small-bore capillary option 3-11 19. Fit a new, metal gasket to the probe tip. 20. Fit the probe tip over the capillary, and screw the tip onto the probe assembly. 21. Use the 10-mm wrench to tighten the probe tip. Caution: to avoid gas leakage, fully tighten the probe tip. Metal gask...

  • Page 56

    3-12 configuring the lockspray source 22. Using the nebulizer adjuster knob, adjust the capillary so that it protrudes by approximately 0.5 mm from the end of the probe tip. Tip: during normal operation, the adjuster knob relies on gas pressure to retract the capillary. To retract the capillary with...

  • Page 57: Configuring For Apci Mode

    Configuring for apci mode 3-13 24. Use the combined 2.5-mm allen wrench to fit and tighten the 3 probe end-cover retaining screws. 25. Fit the esi probe to the source (see page 3-2 ). Configuring for apci mode to operate in apci mode, you must fit the apci probe and corona pin to the lockspray sourc...

  • Page 58

    3-14 configuring the lockspray source to install the apci probe: 1. Prepare the instrument for working on the source (see page 5-7 ). 2. With the probe label facing you, carefully slide the apci probe into the hole in the probe adjuster assembly, ensuring that the probe location dowel aligns with th...

  • Page 59

    Configuring for apci mode 3-15 3. Tighten the probe locking ring to secure the probe in place. Tip: an automatic pressure test is performed when the probe is correctly seated in position. Apci probe mounted on the source enclosure: 4. Open the access door to the intellistart fluidics system (see the...

  • Page 60

    3-16 configuring the lockspray source recommendation: to reduce peak broadening, use 0.004-inch id tubing for sample flow rates 1.2 ml/min; use 0.005-inch id tubing for sample flow rates >1.2 ml/min. Requirements: • if you are replacing the tubing supplied with the instrument, you must minimize the ...

  • Page 61: Removing The Apci Probe

    Configuring for apci mode 3-17 6. Close the access door to the intellistart fluidics system. 7. Install the corona pin in the source (see page 5-11 ). Removing the apci probe required materials chemical-resistant, powder-free gloves to remove the apci probe: 1. Prepare the instrument for working on ...

  • Page 62: Configuring For Esci Mode

    3-18 configuring the lockspray source configuring for esci mode to operate in esci mode, you must fit an esi probe and corona pin to the lockspray source enclosure. The system, with the esi probe installed and corona discharge pin fitted, can alternate between esi and esci modes, facilitating data a...

  • Page 63: Source

    4-1 4 configuring the nanolockspray source the waters nanolockspray dual, electrospray, ion source enables the optimized co-introduction of sample and lock mass compound directly into the ion source. This feature provides authenticated, exact-mass measurement in ms mode at low flow rates. Contents: ...

  • Page 64

    4-2 configuring the nanolockspray source overview of the nanolockspray source nanolockspray source: the nanolockspray source enclosure holds two nanospray probes positioned orthogonally with respect to one another. The sample flows through one probe and the lock-mass reference through the other. A m...

  • Page 65: Sample Sprayer

    Overview of the nanolockspray source 4-3 schematic of the nanolockspray source: spray indexing permits acquiring sample and lock-spray data in separate data channels, and the baffle design ensures negligible cross talk between the two sprays. Data from the lock-spray are used to calculate a correcti...

  • Page 66: Nanoflow Gas Supply

    4-4 configuring the nanolockspray source nanoflow gas supply the nebulizer gas supply pressure for the sample sprayer is electronically controlled from 0 to 2 bar. The optimum pressure is sprayer-dependent, but it usually lies between 0.3 and 1.0 bar. Purge gas purge gas, typically flowing at 100 l/...

  • Page 67: Source Type Selection

    Source type selection 4-5 source type selection to select the source: from the tune window, click source > nanoflow. Warning: to avoid electrical shock when working with capillary electrophoresis (ce) equipment, connect a ce interlock from the rear of the instrument to the ce apparatus (see page b-2...

  • Page 68

    4-6 configuring the nanolockspray source advancing and retracting the sprayer platform to move the sprayer platform out of the source: 1. Confirm that the sprayer’s safety cover is installed (see the figure on page 4-2 ). 2. Unscrew the thumbscrew on the front of the sprayer platform. 3. Pull out th...

  • Page 69

    Adjusting the sprayer tip position 4-7 adjusting the sprayer tip position to adjust the tip position: 1. Adjust the x, y, and z controls on the adjuster assembly to move the sprayer tip close to the sampling cone and baffle. 2. Adjust the height of the sprayer so that its tip is level with the cente...

  • Page 70: Setting Up The Camera

    4-8 configuring the nanolockspray source setting up the camera to set up the camera: 1. In the instrument console, ensure that the nanoflow source is selected. 2. Open the camera control, see the online help for details. Camera control view of sprayers and sample cone: 3. Rotate the camera’s focusin...

  • Page 71

    Optional glass capillary sprayer 4-9 optional glass capillary sprayer the glass capillary sprayer is designed for use with metal-coated borosilicate glass capillaries. They allow extremely low flow rates (less than 100 nl/min). The glass capillaries are used for one sample only and must then be disc...

  • Page 72

    4-10 configuring the nanolockspray source 2. Thread the indicated length of ptfe tubing through each of the “finger-tight” nuts, and tighten them into the tee piece in the arrangement as shown. 3. Using the sealtight nut and ferrule, connect the 0.023-inch id ptfe tubing to the pressure controller c...

  • Page 73

    Optional glass capillary sprayer 4-11 3. Retract the sprayer platform adjuster assembly from the source (see page 4-6 ). 4. Remove the sprayer’s safety cover. 5. Place the sprayer (with gas line fitted) on the platform, and secure it with the thumbscrew. 6. Refit the safety cover. Fitting and loadin...

  • Page 74

    4-12 configuring the nanolockspray source 2. Carefully remove the capillary from its case by lifting it vertically while pressing down on the foam with two fingers. 3. Over the square-cut end of the capillary, pass first the knurled nut, then approximately 5 mm of conductive elastomer, and finally t...

  • Page 75

    Optional glass capillary sprayer 4-13 6. Load sample into the capillary using either a fused silica syringe needle or a geloader tip. Tip: shake the loaded capillary to move the liquid to the tip of the sprayer. 7. With the sprayer mounted on the adjuster platform, screw the union back into the asse...

  • Page 76

    4-14 configuring the nanolockspray source 5. While watching the camera image, carefully move the tip forward toward the groove, until it touches, and a small piece of the glass capillary shears off. 6. Return the sprayer to its previous position. 7. Ascertain that sample is flowing by observing a dr...

  • Page 77: Maintenance Procedures

    5-1 5 maintenance procedures this chapter provides the maintenance guidelines and procedures necessary to maintain the instrument’s performance. Keep to a maintenance schedule, and perform maintenance as required and described in this chapter. Contents: topic page maintenance schedule 5-3 spare part...

  • Page 78

    5-2 maintenance procedures replacing the esi probe sample capillary 5-66 cleaning the apci probe tip 5-75 replacing the apci probe sample capillary 5-75 replacing the reference probe capillary (lockspray source) 5-82 replacing the reference probe capillary (nanolockspray source) 5-85 cleaning or rep...

  • Page 79: Maintenance Schedule

    Maintenance schedule 5-3 maintenance schedule the following table lists periodic maintenance schedules that ensure optimum instrument performance. Maintenance schedule: procedure frequency for information... Clean the instrument case. As required. See page 5-17 . Empty the nitrogen exhaust trap bott...

  • Page 80: Spare Parts

    5-4 maintenance procedures spare parts waters recommends that you replace only the parts mentioned in this document. For spare parts details, see the waters quality parts locator on the waters web site’s services/support page. Replace the reference probe capillary. Annually. See page 5-82 . Clean or...

  • Page 81

    Troubleshooting with connections insight 5-5 troubleshooting with connections insight connections insight ® is an “intelligent” device management (idm) web service that enables waters to provide proactive service and support for the acquity uplc system. To use connections insight, you must install i...

  • Page 82: Safety and Handling

    5-6 maintenance procedures safety and handling bear in mind the following safety considerations when performing maintenance procedures: see appendix a for safety advisory information. Warning: the instrument components can be contaminated with biohazardous and/or toxic materials. Always wear chemica...

  • Page 83

    Preparing the instrument for working on the source 5-7 preparing the instrument for working on the source for safety reasons, you must follow the procedure described below before working on the source (for example, when changing the probe, installing or removing the corona pin, operating the source ...

  • Page 84

    5-8 maintenance procedures to remove the source enclosure: 1. Prepare the instrument for working on the source (see page 5-7 ). 2. Remove the probe from the source: • if you are removing an esi probe, see page 3-6 . • if you are removing an apci probe, see page 3-17 . 3. Slide open the instrument’s ...

  • Page 85

    Removal and refitting of the source enclosure 5-9 6. Using both hands, grasp the source enclosure, and lift it vertically off the two supporting studs on the source adaptor housing. 7. Store the cables neatly by plugging them into the cable-storage positions on the rear of the source enclosure. Tp03...

  • Page 86

    5-10 maintenance procedures fitting the source enclosure to the instrument required materials chemical-resistant, powder-free gloves to fit the source enclosure to the instrument: 1. Using both hands, fit the source enclosure to the two supporting studs on the source adaptor housing. 2. Close the so...

  • Page 87

    Installing and removing the corona pin 5-11 installing and removing the corona pin for apci, esci, and dual-mode appi/apci operation, you must fit a corona pin to the source. Installing the corona pin in the source required materials chemical-resistant, powder-free gloves to install the corona pin i...

  • Page 88

    5-12 maintenance procedures corona pin mounting contact: 4. Fit the corona pin to the corona pin mounting contact, ensuring that the corona pin is securely mounted and that its tip aligns with the sample cone orifice. Corona pin: warning: the corona pin tip is sharp. To avoid puncture wounds, handle...

  • Page 89

    Installing and removing the corona pin 5-13 5. Close the source enclosure. 6. Look through the source window, and use the vernier probe adjuster (see page 3-4 ) to position the esi probe tip so that it is pointing, approximately, midway between the tips of the sample cone and corona pin. Removing th...

  • Page 90

    5-14 maintenance procedures operating the source isolation valve you must close the source isolation valve to isolate the source from the instrument vacuum system for certain maintenance procedures. Required materials chemical-resistant, powder-free gloves to close the source isolation valve before ...

  • Page 91

    Operating the source isolation valve 5-15 3. Close the source isolation valve by moving its handle counterclockwise, to the vertical position. Tp03130 isolation valve handle in closed position.

  • Page 92

    5-16 maintenance procedures to open the source isolation valve after completing a maintenance procedure: 1. Open the source isolation valve by moving its handle clockwise to the horizontal position. 2. Close the source enclosure. Warning: the source components can be contaminated with biohazardous a...

  • Page 93: Removing O-Rings and Seals

    Removing o-rings and seals 5-17 removing o-rings and seals when performing certain maintenance procedures, you must remove o-rings or seals from instrument components. An o-ring removal kit is provided with the instrument. O-ring removal kit: to remove an o-ring: use the tools as aids to pull the o-...

  • Page 94

    5-18 maintenance procedures emptying the nitrogen exhaust trap bottle inspect the nitrogen exhaust trap bottle in the instrument exhaust line daily, and empty it before it is more than approximately 10% full. Nitrogen exhaust trap bottle: tp03164 from instrument exhaust connection to laboratory exha...

  • Page 95

    Emptying the nitrogen exhaust trap bottle 5-19 required materials chemical-resistant, powder-free gloves to empty the nitrogen exhaust trap bottle: 1. In the instrument console, click stop flow . 2. Pull the source enclosure release (located at the bottom, right-hand side) outwards, and swing open t...

  • Page 96

    5-20 maintenance procedures inspecting the varian roughing pump oil level note: this procedure is not required for an edwards oil-free roughing pump requirement: you must determine the oil level while the roughing pump is running. The roughing pump oil level appears in the pump’s oil-level sight gla...

  • Page 97

    Adding oil to the varian roughing pump 5-21 adding oil to the varian roughing pump note: this procedure is not required for an edwards oil-free roughing pump. If, on checking the pump’s oil level, you find it low, you must add oil (see page 5-20 ). Varian roughing pump: required materials • chemical...

  • Page 98

    5-22 maintenance procedures to add oil to the roughing pump: 1. Vent and shut down the mass spectrometer (see the mass spectrometer’s online help for details). 2. Disconnect the power cords for the mass spectrometer and roughing pump from the main power source. 3. Allow the oil to settle in the pump...

  • Page 99: Filter

    Replacing the varian roughing pump’s oil and oil mist filter 5-23 7. Connect the power cords for the mass spectrometer and roughing pump to the main power source. 8. Start the mass spectrometer (see page 2-2 ). Tips: after you add oil to the pump, the following situations can occur: • the oil level ...

  • Page 100

    5-24 maintenance procedures 3. Allow the roughing pump to cool. 4. Place the container for catching used oil under the pump’s drain plug (see the figure on page 5-21 ). 5. Unscrew and remove the oil filler plug. 6. Use the 8-mm allen wrench to remove the oil drain plug. 7. Tip the pump toward the dr...

  • Page 101

    Replacing the varian roughing pump’s oil and oil mist filter 5-25 to replace the oil mist filter: 1. Use the 3-mm allen wrench to remove the 4 screws securing the oil mist canister cover to the oil mist canister. 2. Remove the oil mist canister cover from the oil mist canister. Tip: the cover can be...

  • Page 102: To Fill The Pump With Oil

    5-26 maintenance procedures 3. Remove the spring and oil mist filter from the canister. 4. Dispose of the oil mist filter in accordance with local environmental regulations. 5. Install the new oil mist filter in the canister. Requirement: to seat the new filter properly, press it down firmly when in...

  • Page 103

    Replacing the varian roughing pump’s oil and oil mist filter 5-27 filling the pump with oil: 1. Use the 8-mm allen wrench to fit, and tighten the roughing pump’s oil drain plug. Tip: when the oil drain plug is tightened, the plug seals with an o-ring. Compression is controlled by the o-ring groove d...

  • Page 104

    5-28 maintenance procedures 4. Connect the power cords for the mass spectrometer and roughing pump to the main power source. 5. Start the mass spectrometer (see page 2-2 ). Tip: after you add oil to the pump, the following situations can occur: • the oil level drops slightly during the first month o...

  • Page 105

    Cleaning the sampling cone assembly 5-29 cleaning the sampling cone assembly you can remove the sampling cone assembly (comprising the sample cone, o-ring, and cone gas nozzle) for cleaning without venting the instrument. Removing the sampling cone assembly from the source required materials chemica...

  • Page 106

    5-30 maintenance procedures 3. Slide the sampling cone assembly out of the ion block assembly. Caution: do not open the isolation valve at any time when the sampling cone assembly has been removed from the ion block assembly. Tp03131 cone gas nozzle handle sampling cone assembly t p 0 3 1 3 2 ion bl...

  • Page 107

    Cleaning the sampling cone assembly 5-31 disassembling the sampling cone assembly required materials • chemical-resistant, powder-free gloves • combined 2.5-mm allen wrench and cone extraction tool to disassemble the sampling cone assembly: 1. Retrieve the combined 2.5-mm allen wrench and cone extra...

  • Page 108

    5-32 maintenance procedures 2. Slide the collar to the end of the tool. 3. Insert the collar in the sample cone. Collar.

  • Page 109

    Cleaning the sampling cone assembly 5-33 4. Rotate and lift the tool and collar to remove the sample cone from the cone gas nozzle. 5. Remove the o-ring from the sample cone. 6. If the o-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations. 7....

  • Page 110

    5-34 maintenance procedures cleaning the sample cone and cone gas nozzle required materials • chemical-resistant, powder-free gloves. • appropriately sized glass vessels in which to completely immerse components when cleaning. Use only glassware not previously cleaned with surfactants. • hplc-grade ...

  • Page 111

    Cleaning the sampling cone assembly 5-35 4. If you used formic acid in the cleaning solution, do as follows: a. Rinse the components by immersing them in separate glass vessels containing water and then placing the vessels in the ultrasonic bath for 20 minutes. B. Remove any residual water from the ...

  • Page 112

    5-36 maintenance procedures assembling the sampling cone assembly required materials chemical-resistant, powder-free gloves to assemble the sampling cone assembly: 1. Fit the cone gas nozzle handle to the cone gas nozzle and turn the handle clockwise to tighten. 2. Carefully fit the sample cone into...

  • Page 113

    Cleaning the sampling cone assembly 5-37 to fit the sampling cone assembly to the source: 1. Ensure that the source isolation valve is in the closed position (see page 5-14 ). 2. Hold the sampling cone assembly so that the cone gas nozzle handle is oriented horizontally and at the top, and then slid...

  • Page 114: Cleaning The Extraction Cone

    5-38 maintenance procedures 3. Grasp the cone gas nozzle handle, and use it to rotate the sampling cone assembly 90 degrees, moving the handle downward from the horizontal to the vertical position. 4. Open the source isolation valve (see page 5-16 ). 5. Close the source enclosure. Cleaning the extra...

  • Page 115

    Cleaning the extraction cone 5-39 3. Remove the source enclosure (see page 5-7 ). Rationale: removing the source enclosure aids access to the ion block assembly. 4. Use the combined 2.5-mm allen wrench and cone extraction tool to unscrew the 4 captive ion block assembly securing screws. Warning: the...

  • Page 116

    5-40 maintenance procedures 5. Remove the ion block assembly from the peek ion block support. Tp03130 ion block assembly peek ion block support.

  • Page 117

    Cleaning the extraction cone 5-41 removing the extraction cone from the ion block required materials • chemical-resistant, powder-free gloves • combined 2.5-mm allen wrench and cone extraction tool to remove the extraction cone from the ion block: 1. On the rear of the ion block, use the combined 2....

  • Page 118: Cleaning The Extraction Cone

    5-42 maintenance procedures 2. Remove the extraction cone from the ion block. 3. Remove the extraction cone handle insulator from the extraction cone handle. Cleaning the extraction cone required materials • chemical-resistant, powder-free gloves. • appropriately sized glass vessel in which to compl...

  • Page 119

    Cleaning the extraction cone 5-43 • ultrasonic bath. • source of oil-free, inert gas (for example, nitrogen) for drying (air-drying optional). • wash bottle containing hplc-grade (or better) 1:1 methanol/water. • large beaker. To clean the extraction cone: 1. Immerse the extraction cone in the glass...

  • Page 120

    5-44 maintenance procedures 5. Inspect the extraction cone for persisting contamination. If contamination is present, do as follows: a. Use the wash bottle containing 1:1 methanol/water to rinse the extraction cone over the large beaker. B. Blow dry the extraction cone with inert, oil-free gas. 6. I...

  • Page 121

    Cleaning the extraction cone 5-45 fitting the ion block assembly to the source assembly required materials • chemical-resistant, powder-free gloves • combined 2.5-mm allen wrench and cone extraction tool to fit the ion block assembly to the source assembly: 1. Fit the ion block assembly to the peek ...

  • Page 122

    5-46 maintenance procedures cleaning the ion block assembly clean the ion block assembly if cleaning the sample cone, cone gas nozzle, and extraction cone fails to increase signal sensitivity. Disassembling the source ion block assembly required materials • chemical-resistant, powder-free gloves • c...

  • Page 123

    Cleaning the ion block assembly 5-47 3. Grasp the cone gas nozzle handle and use it to rotate the sampling cone assembly through 90 degrees. 4. Slide the sampling cone assembly out of the ion block assembly. 5. Use the combined 2.5-mm allen wrench and cone extraction tool to loosen the 2 ion block c...

  • Page 124

    5-48 maintenance procedures 7. Grasp the isolation valve and pull it out of the ion block. 8. Use the o-ring removal kit to carefully remove the isolation valve o-ring (see page 5-17 ). 9. If the isolation valve o-ring shows signs of deterioration or damage, dispose of it in accordance with local en...

  • Page 125

    Cleaning the ion block assembly 5-49 11. Use the needle-nose pliers to grasp the peek terminal block and partially lift it out of the ion block. 12. Holding the peek terminal block, gently use the needle-nose pliers to gently grasp the heat-shrink tubing on the heater cartridge assembly, and careful...

  • Page 126

    5-50 maintenance procedures 13. Use the o-ring removal kit to carefully remove the cover seal from the ion block (see page 5-17 ). 14. Use the o-ring removal kit to carefully remove the cone gas o-ring from the ion block. 15. If the cover seal or cone gas o-ring shows signs of deterioration or damag...

  • Page 127

    Cleaning the ion block assembly 5-51 16. Insert the combined 2.5-mm allen wrench and cone extraction tool through the hole in the ion block blanking plug, and then unscrew and remove the ion block blanking plug and associated o-ring. 17. If the blanking plug o-ring shows signs of deterioration or da...

  • Page 128

    5-52 maintenance procedures 18. Use the combined 2.5-mm allen wrench and cone extraction tool to remove the captive screws securing the 2 peek extraction cone retainer clips. 19. Remove the extraction cone from the ion block. Caution: • take great care not to damage the extraction cone aperture when...

  • Page 129

    Cleaning the ion block assembly 5-53 20. Remove the extraction cone handle insulator from the extraction cone handle. 21. Remove the extraction cone seal from the ion block. Cleaning the ion block components required materials • chemical-resistant, powder-free gloves. • appropriately sized glass ves...

  • Page 130

    5-54 maintenance procedures to clean the ion block components: 1. Immerse the ion block and isolation valve in separate glass vessels containing 1:1 methanol/water. Tip: if the components are obviously contaminated, use 45:45:10 methanol/water/formic acid. 2. Place the vessels in the ultrasonic bath...

  • Page 131

    Cleaning the ion block assembly 5-55 6. Inspect each component for persisting contamination. Requirement: if contamination is present, repeat the cleaning procedure. If contamination persists, dispose of the component, and obtain a new one before reassembling the ion block assembly. Assembling the s...

  • Page 132

    5-56 maintenance procedures 5. Use the combined 2.5-mm allen wrench and cone extraction tool to tighten the captive screw securing each extraction cone retainer clip to the ion block. 6. Fit the blanking plug o-ring (a new one, if you disposed of the old o-ring) to the ion block blanking plug. 7. Fi...

  • Page 133

    Cleaning the source hexapole assembly 5-57 17. Hold the sampling cone assembly so that the cone gas nozzle handle is oriented horizontally and at the top, and then slide the sampling cone assembly into the ion block assembly. 18. Grasp the sampling cone assembly handle, and use it to rotate the samp...

  • Page 134

    5-58 maintenance procedures 2. Use the 3-mm allen wrench to unscrew and remove the 4 screws securing the peek ion block support to the adaptor housing. 3. Remove the peek ion block support from the adaptor housing. 4. Use the o-ring removal kit to carefully remove all the o-rings from the peek ion b...

  • Page 135

    Cleaning the source hexapole assembly 5-59 cleaning the hexapole assembly required materials • chemical-resistant, powder-free gloves. • 500-ml measuring cylinder or appropriately sized glass vessel in which to completely immerse the hexapole when cleaning. Use only glassware not previously cleaned ...

  • Page 136

    5-60 maintenance procedures 3. Use the hook to carefully suspend the hexapole assembly in the glass vessel so that the bottom of the assembly does not touch the bottom of the vessel. 4. Add 1:1 methanol/water to the glass vessel until the hexapole assembly is immersed completely. 5. Place the vessel...

  • Page 137

    Cleaning the source hexapole assembly 5-61 7. Inspect the hexapole assembly for persisting contamination, requirement: if contamination is present, do as follows: a. Use the wash bottle containing methanol to rinse the source hexapole assembly over the large beaker. B. Blow dry the hexapole assembly...

  • Page 138

    5-62 maintenance procedures tip: if contamination is present, use 1:1 methanol/water, applied to a lint-free cloth, to carefully clean the grooves. 3. Fit the o-rings to the peek ion block support. Requirement: fit new o-rings if any of the old o-rings were disposed of. Tip: to fit an o-ring in its ...

  • Page 139

    Replacing the esi probe tip and gasket 5-63 replacing the esi probe tip and gasket replace the esi probe tip if a blockage occurs in the internal metal sheathing through which the stainless steel capillary passes or if the probe tip is damaged. Removing the esi probe tip and gasket required material...

  • Page 140

    5-64 maintenance procedures 1. Remove the esi probe from the source (see page 3-6 ). 2. Use the 10-mm wrench to remove the probe tip. Tip: if the probe tip is difficult to remove, use the 7-mm wrench in conjunction with the 10-mm wrench. Probe tip 10-mm wrench probe tip 7-mm wrench 10-mm wrench.

  • Page 141

    Replacing the esi probe tip and gasket 5-65 3. Remove the metal gasket from the probe tip. 4. Dispose of the metal gasket in accordance with local environmental regulations. 5. If the probe tip is damaged, dispose of it in accordance with local environmental regulations. Fitting the esi probe tip an...

  • Page 142

    5-66 maintenance procedures to fit the esi probe tip and gasket: 1. Fit the new metal gasket into the probe tip. 2. Fit the probe tip over the capillary, and screw the tip onto the probe assembly. 3. Use the 10-mm wrench to tighten the probe tip. 4. Use the nebulizer adjuster knob to adjust the capi...

  • Page 143

    Replacing the esi probe sample capillary 5-67 to remove the existing capillary: 1. Remove the probe from the source (see page 3-6 ). 2. Use the combined 2.5-mm allen wrench and cone extraction tool to remove the 3 probe end cover retaining screws. Warning: the probe and source components can be cont...

  • Page 144

    5-68 maintenance procedures 3. Remove the end cover and gasket from the probe assembly. 4. Unscrew and remove the nebulizer adjuster knob. End-cover nebulizer adjuster knob gasket.

  • Page 145

    Replacing the esi probe sample capillary 5-69 5. Use the 10-mm wrench to remove the probe tip. Tip: if the probe tip is difficult to remove, use the 7-mm wrench in conjunction with the 10-mm wrench. Probe tip 10-mm wrench probe tip 7-mm wrench 10-mm wrench.

  • Page 146

    5-70 maintenance procedures 6. Remove the metal gasket from the probe tip. 7. Remove the peek union/unf coupling assembly and capillary from the probe. 8. Unscrew and remove the knurled collar from the unf coupling. 9. Remove the knurled collar and conductive sleeve from the capillary. 10. Use the 7...

  • Page 147: Installing The New Capillary

    Replacing the esi probe sample capillary 5-71 11. Unscrew the finger-tight peek union from the unf coupling. 12. Remove the ferrule and ptfe liner sleeve from the capillary. 13. Remove the capillary from the unf coupling. 14. Dispose of the capillary, ptfe liner sleeve, and ferrule in accordance wit...

  • Page 148

    5-72 maintenance procedures • metal gasket for the probe tip • sharp knife or peek tubing cutter • safety goggles to install the new capillary: 1. Use the sharp knife or peek tubing cutter to cut an approximately 60-cm (24-inches) length of red peek tubing. Requirement: cut the tubing perpendicular ...

  • Page 149

    Replacing the esi probe sample capillary 5-73 4. Use the needle-nose pliers to slide a new liner sleeve and ferrule onto the capillary. 5. Insert the capillary in the peek union, and ensure that it is fully seated. 6. Screw the unf coupling into the peek union, finger-tight only. 7. Gently tug on th...

  • Page 150

    5-74 maintenance procedures 15. Carefully push the peek union/unf coupling assembly and capillary into the probe assembly so that the locating pin on the unf coupling is fully engaged in the locating slot at the head of the probe assembly. 16. Fit the nebulizer adjuster knob to the peek union/unf co...

  • Page 151: Cleaning The Apci Probe Tip

    Cleaning the apci probe tip 5-75 cleaning the apci probe tip clean the apci probe tip when you detect buffer buildup on the probe tip or when the signal intensity weakens. To clean the apci probe tip: 1. On the instrument console system tree, click xevo g2 qtof > manual optimization. 2. On the manua...

  • Page 152

    5-76 maintenance procedures to remove the existing capillary: 1. Remove the probe from the source (see page 3-17 ). 2. Use the combined 2.5-mm allen wrench and cone extraction tool to remove the 3 probe end cover retaining screws. Warning: the probe and source components can be contaminated with bio...

  • Page 153

    Replacing the apci probe sample capillary 5-77 3. Remove the end cover and gasket. 4. Unscrew and remove the nebulizer adjuster knob. 5. Remove the peek union/unf coupling assembly and capillary from the probe. 6. Use the 7-mm wrench to loosen the locknut. Nebulizer adjuster knob end cover gasket pe...

  • Page 154: Installing The New Capillary

    5-78 maintenance procedures 7. Unscrew the finger-tight peek union from the unf coupling. 8. Remove the ferrule from the capillary. 9. Remove the capillary from the unf coupling. 10. Dispose of the capillary and ferrule in accordance with local environmental regulations. Installing the new capillary...

  • Page 155

    Replacing the apci probe sample capillary 5-79 to install the new capillary: 1. Use the sharp knife or peek tubing cutter to cut an approximately 60-cm (24-inches) length of red peek tubing. Requirement: cut the tubing perpendicular to its horizontal axis. 2. Insert one end of the red, peek tubing i...

  • Page 156

    5-80 maintenance procedures 8. Use the 7-mm wrench to tighten the locknut against the peek union. 9. Perform a leak test by attaching the free end of the peek tubing to an lc pump and pumping 50:50 acetonitrile/water through it, at 1 ml/min. • if leakage occurs, disassemble and remake the connection...

  • Page 157

    Replacing the apci probe sample capillary 5-81 18. Use the combined 2.5-mm allen wrench and cone extraction tool to fit and tighten the 3 end-cover securing screws. 19. Fit the probe heater (see page 5-93 , step 1 through step 3 ). 20. Fit the probe to the instrument (see page 3-13 ). 21. In the ins...

  • Page 158: Source)

    5-82 maintenance procedures replacing the reference probe capillary (lockspray source) replace the reference probe capillary if it becomes blocked, and you cannot clear it, or if it becomes contaminated or damaged. When replacing the reference probe capillary, you can also replace the microfilter. T...

  • Page 159

    Replacing the reference probe capillary (lockspray source) 5-83 to remove the existing capillary: 1. Prepare the instrument for working on the source (see page 5-7 ). 2. If an esi probe is fitted to the source, remove it from the source (see page 3-6 ). 3. Pull the source enclosure release (located ...

  • Page 160: Installing The New Capillary

    5-84 maintenance procedures 8. Unscrew the finger-tight nut on the rear of the reference sprayer assembly. 9. Remove the liner tubing and capillary from the reference sprayer assembly. 10. Remove the capillary from the liner tubing. 11. Dispose of the capillary in accordance with local environmental...

  • Page 161: Source)

    Replacing the reference probe capillary (nanolockspray source) 5-85 6. Rotate the reference sprayer assembly 90º counterclockwise to lock it in place. 7. Use the extender tool to tighten the sealtight nut on the inside of the source enclosure. 8. Close the source enclosure. Replacing the reference p...

  • Page 162

    5-86 maintenance procedures to remove the reference probe: 1. Prepare the instrument for working on the source (see page 5-7 ). 2. Disconnect the reference probe’s cable from the instrument’s high voltage connector. 3. Disconnect the fused silica capillary from the instrument’s intellistart fluidics...

  • Page 163

    Replacing the reference probe capillary (nanolockspray source) 5-87 6. Unscrew the tapertip peek coupler, and remove the tapertip from the union. 7. Unscrew the capillary peek coupler, and remove the fused-silica capillary from the union. 8. Where appropriate, remove the protective peek sleeve from ...

  • Page 164

    5-88 maintenance procedures installing the new tapertip and capillary required materials • chemical-resistant, powder-free gloves • combined 2.5-mm allen wrench and cone extraction tool • fused-silica cutting tool • pin-plug capillary locator • tapertip • a 375-mm length of 25-µl, fused-silica capil...

  • Page 165

    Replacing the reference probe capillary (nanolockspray source) 5-89 4. Locate the peek coupler in the union, and carefully slide the fused-silica capillary into the union until it butts against the pin-plug capillary locator. Rationale: the pin-plug capillary locator correctly locates the capillary ...

  • Page 166

    5-90 maintenance procedures cleaning or replacing the corona pin required materials • chemical-resistant, powder-free gloves • needle-nose pliers • hplc-grade (or better) methanol • lint-free tissue • lapping film • corona pin to clean or replace the corona pin: 1. Remove the corona pin from the sou...

  • Page 167

    Replacing the apci probe heater 5-91 replacing the apci probe heater replace the apci probe heater it fails to heat the probe. Removing the apci probe heater required materials chemical-resistant, powder-free gloves to remove the apci probe heater: 1. Remove the probe from the source (see page 3-17 ...

  • Page 168

    5-92 maintenance procedures 2. Gripping the probe heater as shown, carefully pull it off the probe assembly. 3. Dispose of the probe heater in accordance with local environmental regulations. Caution: to avoid damaging the probe heater’s electrical connections, do not twist the heater when removing ...

  • Page 169

    Replacing the apci probe heater 5-93 fitting the new apci probe heater required materials • chemical-resistant, powder-free gloves • apci probe heater to fit the new apci probe heater: 1. Use the probe adjuster knob to adjust the capillary so that it protrudes approximately 0.5 mm from the end of th...

  • Page 170

    5-94 maintenance procedures replacing the ion block source heater replace the ion block source heater if it fails to heat the ion block when the instrument is pumped-down (evacuated). Required materials • chemical-resistant, powder-free gloves • needle-nose pliers • combined 2.5-mm allen wrench and ...

  • Page 171

    Replacing the ion block source heater 5-95 3. Use the combined 2.5-mm allen wrench and cone extraction tool to loosen the 2 captive screws securing the ion block cover plate. 4. Remove the ion block cover plate. 5. Use the combined 2.5-mm allen wrench and cone extraction tool to remove the 2 screws ...

  • Page 172

    5-96 maintenance procedures 6. Use the needle-nose pliers to carefully swing the ring terminal tags out of the terminal block. 7. Use the needle-nose pliers to gently grasp the heat-shrink tubing on the heater cartridge assembly and slide the assembly out of the ion block. Ring terminal tag heat-shr...

  • Page 173

    Replacing the ion block source heater 5-97 8. Dispose of the heater cartridge assembly. 9. Use the needle-nose pliers to gently grasp the heat-shrink tubing on the new heater cartridge assembly and slide the assembly into the ion block. 10. Use the needle-nose pliers to position the 2 heater wire ri...

  • Page 174: Enclosure Seals

    5-98 maintenance procedures replacing the lockspray source’s assembly seals note: you do not need to replace the nanolockspray source’s assembly seals; this section relates to the lockspray source only. To avoid excessive leakage of solvent vapor into the laboratory atmosphere, the following seals m...

  • Page 175

    Replacing the lockspray source’s assembly seals 5-99 3. Use the o-ring removal kit to carefully remove the following seals from the source enclosure: • source enclosure seal • nebulizer gas seal • desolvation gas seal probe adjuster nebulizer gas seal probe adjuster assembly probe seal.

  • Page 176: Probe Seals

    5-100 maintenance procedures 4. Dispose of all the seals in accordance with local environmental regulations. Fitting the new source enclosure and probe adjuster assembly probe seals required materials • chemical-resistant, powder-free gloves • wash bottle containing hplc-grade (or better) 1:1 methan...

  • Page 177

    Replacing the lockspray source’s assembly seals 5-101 to fit the new source enclosure and probe adjuster assembly probe seals: 1. Ensure that all the grooves for seals are free from dirt and debris. Tip: if contamination is present, use 1:1 methanol/water, applied to a lint-free cloth, to carefully ...

  • Page 178

    5-102 maintenance procedures replacing the mass spectrometer’s air filters replacing the air filter inside the front door required materials • needle-nose pliers • new filter to replace the air filter inside the front door: 1. Open the access door to the fluidics pump (see the figure on page 1-4 ). ...

  • Page 179

    Replacing the mass spectrometer’s air filters 5-103 3. Remove the filter cover from the instrument. 4. Lift the filter, vertically, from its slot in the instrument. Tip: if necessary, use the needle-nose pliers to grasp the filter. 5. Dispose of the filter. 6. Fit the new filter into the instrument....

  • Page 180

    5-104 maintenance procedures replacing the air filters on the sides of the instrument air filters are located on both sides of the instrument. These filters are retained by louvered panels, which you must remove to access the filters. Filter panel on the right-hand side of the instrument: note: two ...

  • Page 181

    Replacing the mass spectrometer’s air filters 5-105 required materials • m4 pozidriv ® screwdriver • new filters to replace the air filters on the sides of the instrument: for each filter: 1. Use the m4 pozidriv screwdriver to remove the screws securing the louvered panel to the instrument enclosure...

  • Page 182

    5-106 maintenance procedures replacing the intellistart fluidics tubing you must replace the tubing between the components of the intellistart fluidics system when the tubing or its connections become blocked. The following procedures explain how to replace the tubing for the lock-spray and sample d...

  • Page 183

    Replacing the intellistart fluidics tubing 5-107 removing the intellistart fluidics tubing this procedure explains how to remove the intellistart fluidics tubing and disconnect the probe tubing at the diverter valve or grounded union. Required materials chemical-resistant, powder-free gloves to remo...

  • Page 184

    5-108 maintenance procedures plumbing the intellistart fluidics lock-spray system this section explains how to plumb the lock-spray system. Tubing schematic for the lock-spray system: tubing dimensions—lock-spray system: port number connection id (inch) od (inch) color length (mm) 1 reservoir bottle...

  • Page 185

    Replacing the intellistart fluidics tubing 5-109 required materials • chemical-resistant, powder-free gloves. • the xevo g2 qtof fluidics tubing and fitting kit. Tip: this kit contains components for both the sample and lock-spray system plumbing. • for the lockspray reference probe connection – 375...

  • Page 186

    5-110 maintenance procedures peek nut, super flangeless ferrule, and stainless steel (ss) ring: long “finger-tight” fitting: 3. Using a long “finger-tight” fitting, connect orange, 1/16-inch, 680-mm, peek tubing from port 1 of the lock-spray selector valve, through tubing guide a and into reservoir ...

  • Page 187

    Replacing the intellistart fluidics tubing 5-111 requirement: as the tubing emerges from the tubing guide, thread a long “finger-tight” fitting over it. Push the tubing through the left-hand hole, to the bottom of the reservoir bottle, and tighten the fitting. Tips: follow these suggestions if you f...

  • Page 188

    5-112 maintenance procedures 5. Using a long “finger-tight” fitting, connect orange, 1/16-inch, 680-mm, peek tubing from port 3 of the lock-spray selector valve selector valve through tubing guide c and into reservoir bottle c. Requirement: as the tubing emerges from the tubing guide, thread the lon...

  • Page 189

    Replacing the intellistart fluidics tubing 5-113 8. Using a long “finger-tight” peek nut and 1/32-inch peek ferrule, connect the red, 1/32-inch, 200-mm, peek tubing from port 4 of the lock-spray selector valve to the left-hand side of the flow sensor, where you use the 1/32-inch, 6-40 valco™ compres...

  • Page 190

    5-114 maintenance procedures 9. Using a 1/32-inch, valco component fitting assembly, connect the red, 1/32-inch, 60-mm, peek tubing between the flow sensor and the grounded union, where you use a use a short “finger-tight” nut and 1/32-inch ferrule. Short “finger-tight” nut and 1/32-inch ferrule: a ...

  • Page 191

    Replacing the intellistart fluidics tubing 5-115 10. Connect the grounded union to the source’s reference probe. • for the lockspray source, use 1/16-inch peek tubing connected as follows: – at the grounded union end, use a long “finger-tight” fitting. Long “finger-tight” fitting: – at the source re...

  • Page 192

    5-116 maintenance procedures plumbing the intellistart fluidics sample delivery system this section explains how to plumb the sample delivery system for standard flow applications. For low flow, nanoacquity applications, the column outlet passes directly to the nanoflow sprayer, bypassing the intell...

  • Page 193

    Replacing the intellistart fluidics tubing 5-117 required materials • chemical-resistant, powder-free gloves • the xevo g2 qtof fluidics tubing and fitting kit tip: this kit contains components for both the sample and lock-spray system’s plumbing. • needle-nose pliers to plumb the analyte system: re...

  • Page 194

    5-118 maintenance procedures a. Slide the peek nut, stainless steel ring, and super flangeless ferrule over the pump end of the steel tubing. B. Insert the tubing in the pump, and tighten the fittings. C. At the sample selector valve, screw the natural-color peek female-to-male adaptor into port 7. ...

  • Page 195

    Replacing the intellistart fluidics tubing 5-119 3. Using a long “finger-tight” fitting, connect 1/16-inch, 1000-mm, natural-colored peek tubing to port 1 of the sample selector valve, and thread the tubing into the waste port. Long “finger-tight” fitting: tip: the liquid waste system collects waste...

  • Page 196

    5-120 maintenance procedures 5. Using a long “finger-tight” fitting, connect orange, 1/16-inch, 680-mm, peek tubing from port 4 of the sample selector valve, through tubing guide a and into reservoir bottle a. Requirement: as the tubing emerges from the tubing guide, thread the long “finger-tight” f...

  • Page 197

    Replacing the intellistart fluidics tubing 5-121 7. Using two long “finger-tight” fittings, connect the red, 1/16-inch, 200-mm, peek tubing from port 3 of the sample selector valve to port 3 of the diverter valve. 8. Using a long “finger-tight” fitting, connect 1/16-inch, 1000-mm, natural-colored pe...

  • Page 198

    5-122 maintenance procedures 9. Connect port 2 of the diverter valve to the source probe, observing the relevant procedure: • for the esi probe, see page 3-2 . • for the apci probe, see page 3-13 . 10. Close the access doors to the intellistart fluidics system. Caution: ensure that the tubing does n...

  • Page 199: Safety Advisories

    A-1 a safety advisories waters ® instruments display hazard symbols designed to alert you to the hidden dangers of operating and maintaining the instruments. Their corresponding user guides also include the hazard symbols, with accompanying text statements describing the hazards and telling you how ...

  • Page 200: Warning Symbols

    A-2 safety advisories warning symbols warning symbols alert you to the risk of death, injury, or seriously adverse physiological reactions associated with an instrument’s use or misuse. Heed all warnings when you install, repair, and operate waters instruments. Waters assumes no liability for the fa...

  • Page 201: Specific Warnings

    Warning symbols a-3 specific warnings the following warnings can appear in the user manuals of particular instruments and on labels affixed to them or their component parts. Burst warning this warning applies to waters instruments fitted with nonmetallic tubing. Warning: (risk of personal exposure t...

  • Page 202

    A-4 safety advisories mass spectrometer flammable solvents warning this warning applies to instruments operated with flammable solvents. Mass spectrometer shock hazard this warning applies to all waters mass spectrometers. This warning applies to certain instruments when they are in operate mode. Wa...

  • Page 203: Caution Symbol

    Caution symbol a-5 biohazard warning this warning applies to waters instruments that can be used to process material that can contain biohazards: substances that contain biological agents capable of producing harmful effects in humans. Chemical hazard warning this warning applies to waters instrumen...

  • Page 204

    A-6 safety advisories warnings that apply to all waters instruments when operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Attention: changes or modifications to this unit not expressly approved by the party responsible for compliance cou...

  • Page 205

    Warnings that apply to all waters instruments a-7 warning: use caution when working with any polymer tubing under pressure: • always wear eye protection when near pressurized polymer tubing. • extinguish all nearby flames. • do not use tubing that has been severely stressed or kinked. • do not use n...

  • Page 206

    A-8 safety advisories attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: • indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • spegnere tutte le fiamme vive nell'ambiente circostante. • non utilizzare tubi eccessi...

  • Page 207

    Warnings that apply to all waters instruments a-9 警告：当有压力的情况下使用管线时，小心注意以下几点： • 当接近有压力的聚合物管线时一定要戴防护眼镜。 • 熄灭附近所有的火焰。 • 不要使用已经被压瘪或严重弯曲的管线。 • 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。 • 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。 경고 : 가압 폴리머 튜브로 작업할 경우에는 주의하십시오 . • 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오 . • 근처의 화기를 모두 끄십시오 . • 심하게...

  • Page 208

    A-10 safety advisories warning: the user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention: l’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par ...

  • Page 209: Electrical Symbols

    Electrical and handling symbols a-11 electrical and handling symbols electrical symbols these can appear in instrument user manuals and on the instrument’s front or rear panels. Electrical power on electrical power off standby direct current alternating current protective conductor terminal frame, o...

  • Page 210: Handling Symbols

    A-12 safety advisories handling symbols these handling symbols and their associated text can appear on labels affixed to the outer packaging of waters instrument and component shipments. Keep upright! Keep dry! Fragile! Use no hooks!.

  • Page 211: External Connections

    B-1 b external connections this appendix describes the mass spectrometer’s external connections. Warning: the mass spectrometer is heavy. To avoid injury, use suitable machinery and the supplied harness to lift it. Caution: • contact waters technical service before moving the instrument. • if you mu...

  • Page 212: Connections

    B-2 external connections mass spectrometer external wiring and vacuum connections the instrument’s rear panel connectors are shown below. Mass spectrometer rear panel connectors: caution: the two switches above the roughing pump connector are for use by waters service engineers only; they must be ke...

  • Page 213

    Connecting the varian oil-filled roughing pump b-3 connecting the varian oil-filled roughing pump note: to connect the alternative oil-free roughing pump, see page b-7 . Required materials • chemical-resistant, powder-free gloves • 7-mm nut driver • sharp knife • nw25 center rings (included in the x...

  • Page 214

    B-4 external connections to connect the roughing pump: requirement: orient the pump in a way that allows easy daily access to the oil-level sight glasses on the pump and oil exhaust filter. 1. Place the ptfe drip tray on the floor, within 1 m (3 feet 3 inches) of the back of the instrument. Warning:...

  • Page 215

    Connecting the varian oil-filled roughing pump b-5 2. Place the pump on the ptfe drip tray. 3. Using an nw25 center ring, attach the flanged end of the vacuum hose to the roughing pump inlet flange, and then secure the connection with an nw25 clamp, tightening it with the 7-mm nut driver. 4. Connect...

  • Page 216: Pump

    B-6 external connections making the electrical connections to the varian oil-filled roughing pump roughing pump connections: to make the electrical connections to the oil-filled roughing pump: 1. Connect the roughing pump power cord to the main power source. 2. Connect the relay cable from the rough...

  • Page 217

    Connecting the edwards oil-free roughing pump b-7 connecting the edwards oil-free roughing pump the oil-free roughing pump is an alternative to the oil-filled roughing pump. To connect the oil-filled roughing pump, see page b-3 . Required materials • chemical-resistant, powder-free gloves • 7-mm nut...

  • Page 218

    B-8 external connections to connect the oil-free roughing pump: . 1. Place the pump on the floor, within 1 m (3 feet 3 inches) of the instrument. 2. Using an nw40 center ring, attach the nw25/nw40 adaptor to the roughing pump inlet flange, and then secure the connection with an nw40 clamp, tightenin...

  • Page 219

    Connecting the edwards oil-free roughing pump b-9 3. Using an nw25 center ring, attach the flanged end of the vacuum hose to the nw25/nw40 adaptor, and then secure the connection with an nw25 clamp, tightening it with the 7-mm nut driver. 4. Connect the free end of the vacuum hose to the vacuum port...

  • Page 220: Roughing Pump

    B-10 external connections making the electrical connections to the edwards oil-free roughing pump roughing pump connections: to make the electrical connections for the oil-free roughing pump: 1. Connect the roughing pump power cord to the main power source. 2. Connect the relay cable from the roughi...

  • Page 221

    Connecting to the nitrogen gas supply b-11 to connect the nitrogen gas supply: 1. Connect one free end of the 6-mm ptfe tubing to the nitrogen inlet port on the rear of the instrument. Nitrogen and collision cell gas inlet, and vacuum connectors: 2. Attach a nitrogen regulator to the nitrogen supply...

  • Page 222

    B-12 external connections connecting to the collision cell gas supply required materials • chemical-resistant, powder-free gloves • 7/16-inch wrench • 1/8-inch swagelok ® nut and ferrule • 1/8-inch stainless steel tube (supplied with the mass spectrometer) • argon regulator (not supplied) to connect...

  • Page 223

    Connecting the nitrogen exhaust line b-13 to connect the nitrogen exhaust line: 1. Locate the nitrogen exhaust trap bottle in an accessible area below the instrument. Warning: • biohazardous and/or toxic lc solvents and analytes can be carried in the nitrogen exhaust, which must be vented via the ni...

  • Page 224

    B-14 external connections nitrogen exhaust trap bottle: 2. Cut a length of 4-mm tubing long enough to connect the instrument to the nitrogen exhaust trap bottle. 3. Connect one end of the tubing to the pilot valve port on the instrument’s rear panel. 4. Connect the free end of the tubing to the pilo...

  • Page 225

    Connecting the liquid waste line b-15 6. Connect one end of the tubing to the exhaust port on the instrument’s rear panel. 7. Connect the free end of the tubing to the inlet port on the nitrogen exhaust trap bottle. 8. Cut a second length of 12-mm tubing long enough to connect the nitrogen exhaust t...

  • Page 226

    B-16 external connections 3. Route the waste line to the waste container. If necessary, shorten the waste tube so that its end is above the surface of the waste solvent. Warning: to prevent leakage of biohazardous and/or toxic materials, • do not crimp or bend drain line. A crimp or bend can impede ...

  • Page 227

    Connecting the liquid waste line b-17 positioning of drain tube: tp01807 correct incorrect.

  • Page 228: Connecting The Epc

    B-18 external connections connecting the epc in addition to a workstation pc or instrument server, the xevo g2 tof has an external epc with a real-time operating system that provides the real-time, data-processing required by the analog-digital controller (adc). Mass spectrometer rear panel connecti...

  • Page 229

    Connecting the workstation (systems with no acquity lc) b-19 3. Connect one end of the shielded, crossover, network cable to the lower of the two ethernet ports on the epc. 4. Connect the free end of the shielded, crossover, network cable to the adc ethernet port on the instrument. To connect the ep...

  • Page 230

    B-20 external connections connecting ethernet cables (systems with acquity lc) requirement: use shielded network cables with the mass spectrometer to ensure compliance with fcc limits. To make ethernet connections: 1. Connect one end of one shielded ethernet cable to the network switch, and then con...

  • Page 231

    Input/output signal connectors b-21 input and output connector locations: input/output signal connector configuration: external connections 2 10 1 external connections 1 10 1 system activity 1 2 3 4 5 6 7 8 9 10 + - event in 1 external connections 1 not used ce interlock out + - event in 2 not used ...

  • Page 232

    B-22 external connections external connections 1: pin function rating 1 event in 1+, digital signal, optimum +3.3v max +5v +5 v 2 event in 1-, digital ground, 0v 0 v 3 not used 4 event in 2+, digital signal, optimum +3.3v max +5v +5 v 5 event in 2-, digital ground, 0v 0 v 6 not used 7 not used 8 ce ...

  • Page 233: Signal Connections

    Input/output signal connectors b-23 signal connections requirement: to meet the regulatory requirements of immunity from external electrical disturbances, install connection covers over the signal connectors. Mass spectrometer signal connections: signal connections description analog out used for an...

  • Page 234

    B-24 external connections to make signal connections: 1. Determine the signal connection location from the silk-screened label on the rear panel of each instrument. 2. Attach the positive and negative leads of the signal cable to the connector. 3. Slide the clamp (with the bend facing down) into the...

  • Page 235

    Input/output signal connectors b-25 5. Insert the connector with the signal cable into the connection cover, position the clamp over the cable leads, and tighten the clamp into place with the second self-tapping screw. 6. Place the second connection cover over the first cover, and snap it into place...

  • Page 236

    B-26 external connections connecting to the electricity source the mass spectrometer requires a separate, grounded electricity source. The ground connection in the electrical outlet must be common and connected near the system. To connect to the electricity source: recommendation: use a line conditi...

  • Page 237

    Connecting the nanolockspray source camera b-27 to connect the camera to the workstation: 1. Connect the coaxial cable between the video output connector on the instrument’s rear panel and the interface box. 2. Connect the usb cable between the interface box and any free usb socket on the workstatio...

  • Page 238

    B-28 external connections.

  • Page 239: Compatible Solvents

    C-1 c materials of construction and compatible solvents warning: to confirm the integrity of the source exhaust system, you must address any safety issues raised by the contents of this appendix. Contents: topic page preventing contamination c-2 items exposed to solvent c-2 solvents used to prepare ...

  • Page 240: Preventing Contamination

    C-2 materials of construction and compatible solvents preventing contamination for information on preventing contamination, refer to controlling contamination in uplc/ms and hplc/ms systems (part number 715001307). You can find this document on http://www.Waters.Com; click services and support > sup...

  • Page 241

    Solvents used to prepare mobile phases c-3 solvents used to prepare mobile phases these solvents are the most common ingredients used to prepare mobile phases for reverse-phase lc/ms (api): • water • methanol • acetonitrile • formic acid ( • acetic acid ( • ammonium acetate ( • ammonium formate ( th...

  • Page 242

    C-4 materials of construction and compatible solvents.

  • Page 243: Index

    Index-1 a acquity uplc system 1-3 apci mode 1-8 , 3-13 , 3-18 apci probe cleaning tip 5-3 , 5-75 installing 3-13 removing 3-17 replacing capillary 5-3 , 5-75 heater 5-4 , 5-91 appi/apci source 1-2 applicable symbols vii b biohazard warning a-5 burst warning a-3 c calibrating viii calibration 2-3 cau...

  • Page 244

    Index-2 esi mode 1-7 , 3-2 esi probe capillary, replacing 5-3 , 5-66 installing 3-2 optimizing for esci 3-18 removing 3-6 , 5-85 tip, replacing 5-3 , 5-63 ethernet cables, connecting b-20 external connections b-1 wiring b-2 extraction cone, cleaning 5-38 f fitting ion block assembly 5-45 sampling co...

  • Page 245

    Index-3 i leds 2-3 maintenance procedures 5-1 preparing for working on the source 5-7 rebooting 2-8 source enclosure removing 5-7 state of readiness, verifying 2-3 mass spectrometer shock hazard a-4 materials of construction c-1 mobile phases, solvents used in preparation of c-3 n nanoacquity uplc s...

  • Page 246

    Index-4 ion block heater 5-4 , 5-94 lockspray source assembly seals 5-98 oil-free pump seals 5-3 reference probe capillary 5-4 , 5-85 roughing pump oil 5-3 oil mist filter 5-3 scroll pump seals 5-3 source assembly seals 5-4 varian roughing pump oil 5-23 oil mist filter 5-23 roughing pump oil inspect...

  • Page 247

    Index-5 i inspecting level of 5-20 replacing 5-23 oil level sight glass 5-20 oil mist filter, replacing 5-23 w warning symbols a-2 , a-6 waste liquid disposal 5-19 handling 5-19 waters quality parts locator 5-4 workstation, connecting b-19 x xevo g2 qtof calibration 2-3 emergency shutdown 2-9 leavin...

  • Page 248

    Index-6.