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Zeiss 35 mm SLR Camera Operating Manual

Manual is about: Photomicrography with 35 mm SLR Cameras

Summary of 35 mm SLR Camera

  • Page 1

    Photomicrography with 35 mm slr cameras operating manual.

  • Page 2

    Introduction photomicrography using carl zeiss copyright 35 mm slr cameras 0-2 b 40-046 e 03/99 knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning t...

  • Page 3

    Photomicrography using introduction 35 mm slr cameras contents carl zeiss b 40-046 e 03/99 0-3 contents page introduction ........................................................................................................ 0-1 title page..............................................................

  • Page 4

    Introduction photomicrography using carl zeiss contents 35 mm slr cameras 0-4 b 40-046 e 03/99 chapter 2 start-up.................................................................................................................. 2-1 2 start-up ............................................................

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    Photomicrography using introduction 35 mm slr cameras contents carl zeiss b 40-046 e 03/99 0-5 chapter 4 maintenance, care, troubleshooting and service .................................... 4-1 4 maintenance, care, troubleshooting and service ......................................................... ...

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    Introduction photomicrography using carl zeiss instrument safety 35 mm slr cameras 0-6 b 40-046 e 03/99 notes on instrument safety the following warning and information symbols are used in this manual: ☞ note this symbol is a warning which you must observe under all circumstances. Caution this symbo...

  • Page 7

    Photomicrography using introduction 35 mm slr cameras notes on warranty carl zeiss b 40-046 e 03/99 0-7 notes on warranty: the manufacturer guarantees that the instrument has no material and production defects when delivered. You must inform us of any defects immediately and we must do anything to m...

  • Page 8

    Introduction photomicrography using carl zeiss overall view 35 mm slr cameras 0-8 b 40-046 e 03/99 overall view of 35 mm slr camera on 2.5 × /t2 adapter for slr cameras on 60 mm camera port of the stemi 2000-c stereomicroscope in this example 1 cable release 2 eyepiece cover 3 35 mm slr camera 4 t2 ...

  • Page 9

    Photomicrography using introduction 35 mm slr cameras overall view carl zeiss b 40-046 e 03/99 0-9 overall view of 35 mm slr camera on 2.5 × / t2 adapter on front camera port of the axiovert 25 c 1 front camera port 2 2.5 × / t2 camera adapter 3 t2 adapter 4 35 mm slr camera 5 eyepiece cover 6 cable...

  • Page 10

    Introduction photomicrography using carl zeiss overall view 35 mm slr cameras 0-10 b 40-046 e 03/99 overall view of 35 mm slr camera on 63 mm objective and adapter for microscope camera (image position 44 mm) with 10 × camera eyepiece on 44 mm camera port – i.E. On the axioskop with phototube in thi...

  • Page 11: Instrument Description

    Photomicrography with instrument description 35 mm slr cameras contents carl zeiss b 40-046 e 03/99 1-1 instrument description contents 1 instrument description .......................................................................................... 1-3 1.1 name and intended application .............

  • Page 12

    Instrument description photomicrography with carl zeiss 35 mm slr cameras 1-2 b 40-046 e 03/99.

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    Photomicrography with instrument description 35 mm slr cameras instrument description carl zeiss b 40-046 e 03/99 1-3 1 instrument description 1.1 name and intended application the use of commercially available 35 mm slr cameras on microscopes from carl zeiss is a low-price alternative to photo docu...

  • Page 14

    Instrument description photomicrography with carl zeiss components 35 mm slr cameras 1-4 b 40-046 e 03/99 1.3 35 mm slr camera components fig. 1-1 35 mm slr camera components.

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    Photomicrography with instrument description 35 mm slr cameras components carl zeiss b 40-046 e 03/99 1-5 key to fig. 1: 1 35 mm slr camera housing 2 t2 adapter 3 objective f’ = 63 mm in t2 mount 4 2.5 × /t2 adapter for slr cameras 5 2.5 × slr adapter comp. 6 2.5 × / t2 camera adapters 8, 11 photo e...

  • Page 16

    Instrument description photomicrography with carl zeiss components 35 mm slr cameras 1-6 b 40-046 e 03/99 35 mm slr camera components cat. No. . The following components for the attachment of 35 mm slr cameras to microscopes from carl zeiss have been offered and are continued to be offered:: 1 35 mm...

  • Page 17

    Photomicrography with instrument description 35 mm slr cameras technical data carl zeiss b 40-046 e 03/99 1-7 1.4 overview of image positions (tube adapters), relevant phototubes and microscope stands image position (tube adapter) cat.No. Of phototube microscope stand 60 mm 452902-0000-000 kf 2, sta...

  • Page 18

    Instrument description photomicrography with carl zeiss technical data 35 mm slr cameras 1-8 b 40-046 e 03/99 image position (tube adapter) cat.No. Of phototube microscope stand 44 mm 452910-0000-000 452911-0000-000 452912-0000-000 452914-0000-000 axioskop / axioskop 20 451722-0000-000 axiovert 10 /...

  • Page 19

    Photomicrography with instrument description 35 mm slr cameras technical data carl zeiss b 40-046 e 03/99 1-9 1.5 technical data dimensions (outer diameter × depth / height) 35mm slr camera housing contax 167 mt ....................................................Approx. 150 × 94 × 52 mm 35mm slr ca...

  • Page 20

    Instrument description photomicrography with carl zeiss technical data 35 mm slr cameras 1-10 b 40-046 e 03/99 power supply for 35 mm slr camera housing contax 167 mt ................................................................................................... 4 batteries aaa / 1.5 v contax ar...

  • Page 21: Start-Up

    Photomicrography with start-up 35 mm slr cameras contents carl zeiss b 40-046 e 03/99 2-1 start-up contents 2 start-up....................................................................................................................... 2-2 2.1 unpacking the photomicrography components................

  • Page 22

    Start-up photomicrography with carl zeiss photomicrography components/photo reticles 35 mm slr cameras 2-2 b 40-046 e 03/99 2 start-up the components for photomicrography with 35 mm slr cameras can be installed, converted and started up by the customer himself. However, if requested, it is also poss...

  • Page 23

    Photomicrography with start-up 35 mm slr cameras photo reticles carl zeiss b 40-046 e 03/99 2-3 photo reticles cat. No. Eyepieces cat. No. Mc 2.5 × / d = 26 mm 454075-0000-000 pl 10 × / 18 br. Foc. E-pl 10 × / 20 br. Foc. W 10 × / 21 foc. Pl 10 × /23 br. Foc. W-pl 10 × / 23 br. Foc. E-pl 10 × / 23 b...

  • Page 24

    Start-up photomicrography with carl zeiss photo reticles 35 mm slr cameras 2-4 b 40-046 e 03/99 2.2.2 insertion of mc 2.5 × photo reticles in the eyepiece a focusing eyepiece with photo reticle must be used if focusing is not to be performed through the viewfinder of the slr camera. The mc 2.5 × pho...

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    Photomicrography with start-up 35 mm slr cameras attachment of 35 mm slr cameras carl zeiss b 40-046 e 03/99 2-5 2.3 attachment of 35 mm slr cameras the attachment of 35 mm slr cameras to various camera ports is described in detail in the following and can be performed by the users themselves. 2.3.1...

  • Page 26

    Start-up photomicrography with carl zeiss attachment of 35 mm slr cameras 35 mm slr cameras 2-6 b 40-046 e 03/99 2.3.2 attachment of 35 mm slr cameras with objective f' = 63 mm to 60 mm and 40 mm camera ports • remove dust cover or lens from the slr camera housing. • attach camera-specific t2 adapte...

  • Page 27

    Photomicrography with start-up 35 mm slr cameras attachment of 35 mm slr cameras carl zeiss b 40-046 e 03/99 2-7 2.3.3 attachment of 35 mm slr cameras with 2.5 × / t2 adapter to front port of axiovert 25 c / cfl / ca • remove dust cover or lens from the slr camera housing. • attach camera-specific t...

  • Page 28

    Start-up photomicrography with carl zeiss attachment of 35 mm slr cameras 35 mm slr cameras 2-8 b 40-046 e 03/99 2.3.4 attachment of 35 mm slr cameras to front camera port of axiovert 35 / 35 m / 135 / 135 m the front camera port (2-6/5) of the axiovert 35 / 35 m / 135 / 135 m is a t2 adapter with c...

  • Page 29

    Photomicrography with start-up 35 mm slr cameras attachment of 35 mm slr cameras carl zeiss b 40-046 e 03/99 2-9 2.3.5 attachment of 35 mm slr cameras with objective f' = 63 mm to camera ports with 40 mm clamping diameter for photomicrography with 35 mm slr cameras on standard, wl and im microscope ...

  • Page 30

    Start-up photomicrography with carl zeiss attachment of 35 mm slr cameras 35 mm slr cameras 2-10 b 40-046 e 03/99 2.4 determination of the exposure time range for unblurred photographs the limits of the exposure time range for unblurred photographs depend on the used slr camera, the microscope adapt...

  • Page 31: Operation

    Photomicrography with operation 35 mm slr cameras contents carl zeiss b 40-046 e 03/99 3-1 operation contents 3 operation ...................................................................................................................3-2 3.1 adaptation and functions .................................

  • Page 32

    Operation photomicrography with carl zeiss adaptation and functions 35 mm slr cameras 3-2 b 40-046 e 03/99 3 operation 3.1 adaptation and functions the adaptation components for 35 mm slr cameras for photomicrography are simple screwing and clamping adapters which are described in chapter 2, start-u...

  • Page 33

    Photomicrography with operation 35 mm slr cameras photography in a few steps carl zeiss b 40-046 e 03/99 3-3 3.2 photomicrography with 35 mm slr cameras 3.2.1 photography in a few steps • carefully set the microscope for observation. If possible, select beam path for simultaneous observation and pho...

  • Page 34

    Operation photomicrography with carl zeiss selection of color temperature/film 35 mm slr cameras 3-4 b 40-046 e 03/99 3.2.2 setting the color temperature to 3200 k and setting the image brightness when using color reversal films for artificial light (3200 k), it is recommended to set the illuminator...

  • Page 35

    Photomicrography with operation 35 mm slr cameras image section/exposure functions carl zeiss b 40-046 e 03/99 3-5 the film cuts must always be performed as stipulated in din 4536 or iso 1977 (also see fig. 3-1). • the film should be cut so that none of the perforation holes are cut open. • the film...

  • Page 36

    Operation photomicrography with carl zeiss selection of color temperature/film 35 mm slr cameras 3-6 b 40-046 e 03/99 example 1: the correction value +1 means that one exposure value (+ 1 ev) is added to the exposure time in brightfield recommended by the automatic exposure control for zero setting ...

  • Page 37

    Photomicrography with operation 35 mm slr cameras image section/exposure functions carl zeiss b 40-046 e 03/99 3-7 ☞ if the structures of microprism ring and split image indicator are not seen in focus, attach the special eyepiece for eyeglass wearers to the viewfinder. − fine object structures and ...

  • Page 38

    Operation photomicrography with carl zeiss exposure functions 35 mm slr cameras 3-8 b 40-046 e 03/99 example 3: the camera in fig. 3-2 measures shutter speeds between t max (here = 16s) and t min (here = 1/4000 s) for iso 100 /21° films and apertures between 4 and 16. The shortest shutter speed t mi...

  • Page 39

    Photomicrography with operation 35 mm slr cameras exposure functions carl zeiss b 40-046 e 03/99 3-9 therefore, only the following exposure modes are possible: − automatic exposure control with open aperture (automatic control with preselected aperture); depending on the image brightness, the camera...

  • Page 40

    Operation photomicrography with carl zeiss exposure functions 35 mm slr cameras 3-10 b 40-046 e 03/99 example 4: the longest shutter speed measured with cameras with t2 adapter (fig. 3-3) for iso 100/21° and the open aperture 1.4 is t max = 2s, and the shortest shutter speed t min = 1/4000 s. The sh...

  • Page 41

    Photomicrography with operation 35 mm slr cameras blurs/color balance carl zeiss b 40-046 e 03/99 3-11 3.2.8 further notes 3.2.8.1 avoid blurs mirror and shutter functions of the slr camera can cause vibration of the photomicrography structure resulting in blurred micrographs. The image will be dull...

  • Page 42

    Operation photomicrography with carl zeiss color balance 35 mm slr cameras 3-12 b 40-046 e 03/99 assessing the color balance • make test exposures of a specimen area with as much empty background as possible in transmitted- light brightfield. • view slides on a standard light box, the light source o...

  • Page 43

    Photomicrography with operation 35 mm slr cameras darkfield and fluorescence photography carl zeiss b 40-046 e 03/99 3-13 3.2.8.3 darkfield and fluorescence photography compared to standard photomicrography, the following special conditions apply to darkfield and fluorescence photography: − the low ...

  • Page 44

    Operation photomicrography with carl zeiss color balance 35 mm slr cameras 3-14 b 40-046 e 03/99 3.2.8.4 computing the magnification − slr connections, 2.5 × / t2 adapters: the magnification of the image on the 35 mm film m film is the product of objective magnification m obj and the factor of the t...

  • Page 45

    Photomicrography with operation 35 mm slr cameras use of digital cameras carl zeiss b 40-046 e 03/99 3-15 3.3 using digital cameras some digital cameras have the same or a similar housing as standard 35 mm slr cameras. The film gate of these cameras contains an area sensor or a scan sensor array ins...

  • Page 46

    Operation photomicrography with carl zeiss color balance 35 mm slr cameras 3-16 b 40-046 e 03/99.

  • Page 47: Service

    Photomicrography with maintenance, care, troubleshooting and service 35 mm slr cameras contents carl zeiss b 40-046 e 03/99 4-1 maintenance, care, troubleshooting and service contents 4 maintenenance, care, troubleshooting and service ....................................4-2 4.1 instrument care.........

  • Page 48

    Maintenance, care, troubleshooting and service photomicrography with carl zeiss instrument care 35 mm slr cameras b 40-046 e 03/99 4-2 4 maintenenance, care, troubleshooting and service 4.1 instrument care maintenance of the photomicrography components attached to the microscopes is limited to the f...

  • Page 49

    Photomicrography with maintenance, care, troubleshooting and service 35 mm slr cameras contents carl zeiss b 40-046 e 03/99 4-3 4.2 instrument maintenance 4.2.1 performing checks • check the battery voltage for operation of the slr camera. If the battery voltage is no longer sufficient, this is indi...

  • Page 50

    Maintenance, care, troubleshooting and service photomicrography with carl zeiss instrument care 35 mm slr cameras b 40-046 e 03/99 4-4 4.4 consumables and tools description cat. No. Application sw 3 ball-headed screwdriver 000000-0069-551 to change tubes and illuminators dust cover g 459312-0000-000...

  • Page 51: Annex

    Photomicrography with annex 35 mm slr cameras contents carl zeiss b 40-046 e 03/99 a-1 annex list of abbreviations ......................................................................................................................... A-2 physical and technical units .................................

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    Annex photomicrography with carl zeiss list of abbreviations 35 mm slr cameras a-2 b 40-046 e 03/99 list of abbreviations b blue b-function ball-function (shutter remains open until release button, formerly a ball, is pressed; see t-function) br. Suitable for eyeglass wearers c camera, cyan ca camer...

  • Page 53

    Photomicrography with annex 35 mm slr cameras list of abbreviations carl zeiss b 40-046 e 03/99 a-3 sw wrench opening t2-adapter standard adapter for 35 mm cameras t-function time function (shutter remains open after first press and closes only after second press, see b-function) t max maximum expos...

  • Page 54

    Annex photomicrography with carl zeiss list of key words 35 mm slr cameras a-4 b 40-046 e 03/99 list of key words page a adjusting aid...................................................................................................................................... 3-6 ambient conditions ...........