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Zeiss Axio Scope.A1 Operating Manual

Manual is about: Microscope for Routine and Entry-Level Research

Summary of Axio Scope.A1

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    Operati ng manual axio scope.A1 microscope for routine an d entry-level r e search.

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    Carl zeiss copyright / trademark axio scope.A1 2 m60-2-0007 e 05/08 a thorough knowledge of these instructions is mandatory in order to operate the machine. Please familiarize yourself with the content and follow the safety instructions by all means. We reserve the right to make changes in the inter...

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    Axio scope.A1 content / figures carl zeiss m60-2-0007 e 05/08 3 content page 1 introduction ................................................................................................... 9 9 1.1 safety guidelines .....................................................................................

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    Carl zeiss content / figures axio scope.A1 4 m60-2-0007 e 05/08 3.3 switching the microscope and its separate illumination fixtures on and off ......... 1 55 3.4 mounting optional components ................................................................................ 1 56 3.4.1 changing the uppe...

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    Axio scope.A1 content / figures carl zeiss m60-2-0007 e 05/08 5 4.2.9 upper stand part hal 100/hbo dic .............................................................................. 1 101 4.2.10 sideport iscp t60n left ....................................................................................

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    Carl zeiss content / figures axio scope.A1 6 m60-2-0007 e 05/08 figures 0 fig. 1-1 caution sign on lower stand part for hal 100- and hal 50-illumination............................. 188h188h 13 1h1h fig. 1-2 caution sign on upper stand part fl-led........................................................

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    Axio scope.A1 content / figures carl zeiss m60-2-0007 e 05/08 7 44h44h fig. 3-41 mounting the magnification changer .................................................................................. 232h232h 58 45h45h fig. 3-42 changing the filters in the reflector module fl p&c........................

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    Carl zeiss content / figures axio scope.A1 8 m60-2-0007 e 05/08 90h90h fig. 4-32 binocular photo tube ........................................................................................................ 278h278h 106 91h91h fig. 4-33 adjusting the viewing height on the binocular tube ...............

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    Introduction axio scope.A1 safety guidelines carl zeiss m60-2-0007 e 05/08 9 1 introduction 1.1 safety guidelines the microscopes axio scope.A1 were engineered, manufactured and tested according to din en 61010-1 (iec 61010-1) and iec 61010-2-101, safety regulations for metering, control and laborat...

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    Introduction carl zeiss safety guidelines axio scope.A1 10 m60-2-0007 e 05/08 the microscope axio scope.A1 and its genuine accessories may only be used for microscopy methods as indicated in this manual. The following guidelines are especially important: the manufacturer admits no liability if the m...

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    Introduction axio scope.A1 safety guidelines carl zeiss m60-2-0007 e 05/08 11 gas discharge lamps, e.G. Hbo 100, emit ultraviolet radiation which may cause eye and skin burns. Never look directly into the beam and avoid any direct skin exposure to the beam. Never operate the microscope without the n...

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    Introduction carl zeiss safety guidelines axio scope.A1 12 m60-2-0007 e 05/08 never look into the light beam of the illumination equipment - with or without optical instruments. Not even if you just want to look at a specimen. Irreparable eye damage may occur! Do not hold any flammable or easily com...

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    Introduction axio scope.A1 safety guidelines carl zeiss m60-2-0007 e 05/08 13 caution signs on lower stand part for hal 100- and hal 50-illumination and on upper stand part fl-led fig. 1-1 caution sign on lower stand part for hal 100- and hal 50-illumination fig. 1-2 caution sign on upper stand part...

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    Introduction carl zeiss warranty axio scope.A1 14 m60-2-0007 e 05/08 1.2 warranty the manufacturer guarantees that the instrument is free of any material and workmanship defects upon delivery. If you become aware of any deficiencies, please contact us immediately and take all necessary precautions i...

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    Introduction axio scope.A1 stand variants carl zeiss m60-2-0007 e 05/08 15 1.3 stand variants the shipment includes five different upper stand parts, three lower stand parts and two stand columns which can be combined according to the following chart. Upper stand part lower stand part upper stand pa...

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    Introduction carl zeiss stand variants axio scope.A1 16 m60-2-0007 e 05/08 upper stand part lower stand part upper stand part backlight with nosepiece 6x bright field, m27 upper stand part fl/hbo with nosepiece 3x bright field, 3x dic, m27 upper stand part fl-led with nosepiece 3x bright field, 3x d...

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    Introduction axio scope.A1 interface diagram carl zeiss m60-2-0007 e 05/08 17 1.4 interface diagram the following figure shows a diagram of the interfaces of the microscope stand. The combination upper stand part fl/hbo and lower stand part with hal 50 illumination serves as demonstration. The inter...

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    Introduction carl zeiss interface diagram axio scope.A1 18 m60-2-0007 e 05/08 fig. 1-3 interface diagram (upper stand part fl/hbo and lower stand part with hal 50 illumination).

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    Introduction axio scope.A1 summary of the controls and functional elements carl zeiss m60-2-0007 e 05/08 19 1.5 summary of the controls and functional elements legend of 285h285h fig. 1-4: 1 eyepieces 2 binocular tube part 3 field diaphragm (non removable or as slider) 4 adjustment aid (on upper sta...

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    Introduction carl zeiss summary of the controls and functional elements axio scope.A1 20 m60-2-0007 e 05/08 fig. 1-4 summary of the controls and functional elements.

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    Technical manual axio scope.A1 intended use carl zeiss m60-2-0007 e 05/08 21 2 technical manual 2.1 intended use the microscopes axio scope.A1 are universal microscopes designed especially for applications in biological and medical fields and for material studies. Depending on the configuration of t...

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    Technical manual carl zeiss systems overview axio scope.A1 22 m60-2-0007 e 05/08 2.2 systems overview.

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    Technical manual axio scope.A1 systems overview carl zeiss m60-2-0007 e 05/08 23.

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    Technical manual carl zeiss systems overview axio scope.A1 24 m60-2-0007 e 05/08.

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    Technical manual axio scope.A1 systems overview carl zeiss m60-2-0007 e 05/08 25.

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    Technical manual carl zeiss systems overview axio scope.A1 26 m60-2-0007 e 05/08.

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    Technical manual axio scope.A1 technical data carl zeiss m60-2-0007 e 05/08 27 2.3 technical data dimensions (width x depth x height) microscope stand axio scope.A1 (no dl illumination) ..................... Approx. 240 mm x 340 mm x 365 mm stand column vario 380 mm ....................................

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    Technical manual carl zeiss technical data axio scope.A1 28 m60-2-0007 e 05/08 transformer hbo 100 w range of application........................................................................................................................ Indoor protection class......................................

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 29 3 startup the customer has the choice to either set up or mount the axio scope.A1 himself before putting it into operation, or have the zeiss customer service do so for a fee. Please read the safety guidelines ca...

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    Startup carl zeiss mounting the standard components axio scope.A1 30 m60-2-0007 e 05/08 3.1.2 mounting the upper stand part on the stand column if you are going to use a microscope stand consisting of an upper stand part and a stand column, please begin by assembling the upper stand part. • remove t...

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 31 3.1.3 mounting the binocular tube / photo tube all binocular tubes listed in the systems overview (chapter 294h294h 2.2) can be mounted on the microscope stand. • loosen the hexagon socket screws ( 295h295h fig. ...

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    Startup carl zeiss mounting the standard components axio scope.A1 32 m60-2-0007 e 05/08 how to insert eyepiece graticules the eyepieces pl 10x/23 br. Foc. Are designed to be used with eyepiece graticules. The slight image shift caused by the added glass is reversed when the zero position is marked b...

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 33 3.1.5 mounting objectives • lower the mechanical stage with the stage carrier to the bottom stop or lower the stage carrier with the dovetail. • remove the dust protection caps ( 310h310h fig. 3-6/2) from the app...

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    Startup carl zeiss mounting the standard components axio scope.A1 34 m60-2-0007 e 05/08 3.1.6 installing and uninstalling "push&click" modules in the reflector insert to install or uninstall the modules, the reflector insert (reflector turret 4- or 6-fold, reflector slider 2-fold) must be removed fr...

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 35 3.1.7 mounting the reflector insert • first remove the cover cap ( 322h322h fig. 3-9/1) by inserting the ball head screwdriver (sw 3 mm) into the slit ( 323h323h fig. 3-9/2). Turn the locking screw counter clockw...

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    Startup carl zeiss mounting the standard components axio scope.A1 36 m60-2-0007 e 05/08 3.1.8 mounting the mechanical stage depending on the type, the mechanical stages can either be stable or they can be rotated and centered. The shift range is 75 mm in x-direction and 50 mm in y-direction. There a...

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 37 b) how to mount the stage • loosen the screw caps ( 338h338h fig. 3-11/2) of the spring box with approx. 3 turns. • put the stage with the dovetail ring notch onto the spring pin ( 339h339h fig. 3-11/1). • press ...

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    Startup carl zeiss mounting the standard components axio scope.A1 38 m60-2-0007 e 05/08 (4) removing and mounting the additional sleeves both gear knobs for the stages with the order no. 432035-9030-000, 432035-9040-000 and 432035-9050-000 are equipped with additional sleeves for an even more sensit...

  • Page 39

    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 39 3.1.9 mechanical stages with friction adjustment drive length and friction for the x- and y-adjustment can be adjusted individually on the standard mechanical stage (432035-9000-000) and the mechanical stage 4320...

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    Startup carl zeiss mounting the standard components axio scope.A1 40 m60-2-0007 e 05/08 (3) adjusting the connecting rod position of the ergo drive the connecting rod position on the ergo drive of the mechanical stage 75x50 r (432035-9010) can be adjusted individually. • remove the stage with the st...

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 41 3.1.10 mounting the led illuminator for transmitted light be careful, led radiation! Led class 2m, do not look into the beam! The led illuminator can only be inserted on the lower stand part for led illumination....

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    Startup carl zeiss mounting the standard components axio scope.A1 42 m60-2-0007 e 05/08 guidelines for the use of the led illuminator accessories: the slider with diffusion disk 10° emits more light for examining objects with the eyepieces. The slider with diffusion disk 80° emits a more homogeneous...

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 43 3.1.12 mounting the condenser • slide the stage carrier with the focusing drive to the upper stop. • open the frontal optic (if switchable) on the condenser with the lever ( 395h395h fig. 3-19/7). Unscrew both ce...

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    Startup carl zeiss mounting the standard components axio scope.A1 44 m60-2-0007 e 05/08 3.1.13 mounting the stage carrier • screw the shoulder screw ( 405h405h fig. 3-20/3) into the appropriate opening: − opening with mark 0: no adapter is mounted for specimen holder expansion ( 406h406h fig. 3-20/3...

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 45 3.1.14 inserting the halogen lamp 12 v, 50 w • switch off the microscope and pull its plug. Wait at least 15 min for the halogen lamp to cool down. • remove the halogen lamp hal 50 ( 411h411h fig. 3-21/3) from th...

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    Startup carl zeiss mounting the standard components axio scope.A1 46 m60-2-0007 e 05/08 3.1.15 halogen lamp hal 100 the halogen lamp hal 100 may serve as light source for transmitted light and reflected light procedures (except fluorescence light) as long as either the lower stand part or the upper ...

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 47 3.1.15.2 adjusting the halogen lamp hal 100 rough setting • after loosening the clamping screw ( 428h428h fig. 3-23/1 or 429h429h fig. 3-23/7), remove the ready-to-operate halogen lamp ( 430h430h fig. 3-24/2) fro...

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    Startup carl zeiss mounting the standard components axio scope.A1 48 m60-2-0007 e 05/08 3.1.15.3 changing the halogen lamp 12 v, 100 w caution hot surface! Danger of burns! Always let the lamp case cool off for at least 15 min.! It is not necessary to remove the lamp case from the stand when replaci...

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 49 3.1.16 inserting the adjustment aid into the upper stand part fl/hbo • remove the cover ( 447h447h fig. 3-26/1) from the mounting aperture of the adjustment aid. • insert the adjustment aid ( 448h448h fig. 3-26/4...

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    Startup carl zeiss mounting the standard components axio scope.A1 50 m60-2-0007 e 05/08 • press the spring lever ( 454h454h fig. 3-28/5) on the heatsink up and pull the heatsink ( 455h455h fig. 3-28/1) off the inserted dummy or the used burner ( 456h456h fig. 3-28/2). Lay it aside carefully. The cor...

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 51 3.1.17.2 mounting hbo 50 illuminator • mount hbo 50 illuminator ( 464h464h fig. 3-29/3) on the connection piece ( 465h465h fig. 3-29/1) of the upper stand part, align it and tighten the clamping screw ( 466h466h ...

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    Startup carl zeiss mounting the standard components axio scope.A1 52 m60-2-0007 e 05/08 3.1.18 hbo 100 illuminator 3.1.18.1 inserting the mercury vapor short arc lamp hbo 103 w/2 for safety reasons, the illuminator hbo 100 and the mercury vapor short arc lamp hbo 103 w/2 are packed separately. The f...

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    Startup axio scope.A1 mounting the standard components carl zeiss m60-2-0007 e 05/08 53 3.1.18.3 adjusting the hbo 100 illuminator the hbo 100 illuminator is available in two variants (manual and automatic adjustment). The self-adjusting hbo 100 (423011-9901-000) adjusts automatically when the trans...

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    Startup carl zeiss connection of the power supply axio scope.A1 54 m60-2-0007 e 05/08 3.2 connection of the power supply 3.2.1 lower stand part for led and hal 50 illumination • connect the microscope’s power supply ( 486h486h fig. 3-35/2) with its power cord to an electrical outlet. The microscope ...

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    Startup axio scope.A1 switching the microscope and its separate illumination fixtures on and off carl zeiss m60-2-0007 e 05/08 55 3.2.3 upper stand part for hal 100/hbo, fl/hbo, fl-led • mount the halogen lamp hal 100 to the stand (see chapter 492h492h 3.1.15.1). • connect the 3-pin hal 100 plug to ...

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    Startup carl zeiss mounting optional components axio scope.A1 56 m60-2-0007 e 05/08 3.4 mounting optional components make sure to unplug the stand and the external power supply units before you begin any mounting work. After finishing working the appropriate components must be put back into function...

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    Startup axio scope.A1 mounting optional components carl zeiss m60-2-0007 e 05/08 57 3.4.2 mounting the intermediate plate for analyzer slider • loosen the hexagon socket screw ( 512h512h fig. 3-40/5) with the ball head screwdriver sw 3 and lift off the tube ( 513h513h fig. 3-40/1). • use the include...

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    Startup carl zeiss mounting optional components axio scope.A1 58 m60-2-0007 e 05/08 3.4.4 mounting the magnification changer • if necessary, remove camera with adapter (or dust protection cover) from the camera port of the photo tube ( 521h521h fig. 3-41/2). • mount the magnification changer ( 522h5...

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    Startup axio scope.A1 mounting optional components carl zeiss m60-2-0007 e 05/08 59 when mounting the appropriate emission filter into the according reflector module, the mark should be aligned with the orientation groove ( 533h533h fig. 3-42/4). This guarantees that the point angle in the reflector...

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    Startup carl zeiss mounting optional components axio scope.A1 60 m60-2-0007 e 05/08 3.4.6 changing the color splitter in the reflector module fl p&c be extremely careful when mounting the filters and the color splitter in order to avoid damaging and soiling the optical components. We advise ordering...

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    Startup axio scope.A1 mounting optional components carl zeiss m60-2-0007 e 05/08 61 the reflecting (coated) side ( 550h550h fig. 3-46/3) of the color splitter has a tapered edge ( 551h551h fig. 3-46/1) or corner ( 552h552h fig. 3-46/2). • put the excitation part of the module ( 553h553h fig. 3-45/1)...

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    Startup carl zeiss mounting optional components axio scope.A1 62 m60-2-0007 e 05/08 3.4.8 mounting and centering the overview fixture • if necessary, remove polarizer or filter holder from the condenser carrier. • hold the overview fixture ( 567h567h fig. 3-48/4) parallel to the bottom of the conden...

  • Page 63

    Startup axio scope.A1 mounting optional components carl zeiss m60-2-0007 e 05/08 63 3.4.9 changing the led module in the upper stand part fl-led • lift the covering caps ( 576h576h fig. 3-49/1) off the upper stand part. • remove the connecting plug ( 577h577h fig. 3-49/3) of the led module to be cha...

  • Page 64

    Startup carl zeiss mounting optional components axio scope.A1 64 m60-2-0007 e 05/08 3.4.10 inserting a modulator disk in the condenser 0.9/1.25 h • remove the condenser ( 580h580h fig. 3-50/1) from the condenser carrier (see chapter 581h581h 3.1.12). If the condenser cannot be lowered sufficiently, ...

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    Startup axio scope.A1 mounting optional components carl zeiss m60-2-0007 e 05/08 65 3.4.11 changing the diaphragm plasdic how to mount the slit-diaphragm (426715-9000- 000): • turn the ph-diaphragm which needs to be changed into the free aperture of the modulator disk. • screw the modulator disk’s c...

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    Startup carl zeiss mounting optional components axio scope.A1 66 m60-2-0007 e 05/08 3.4.13 changing the filter in the filter wheel transmitted light • remove the stage carrier (see chapter 599h599h 3.1.11). • unscrew three screws ( 600h600h fig. 3-53/3) of the field diaphragm socket ( 601h601h fig. ...

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    Startup axio scope.A1 default setting of the microscope carl zeiss m60-2-0007 e 05/08 67 3.5 default setting of the microscope 3.5.1 adjusting the eyepiece distance (distance of the pupils) on the binocular tube • adjust the eyepiece distance (distance of the pupils) according to the individual dist...

  • Page 68

    Startup carl zeiss default setting of the microscope axio scope.A1 68 m60-2-0007 e 05/08 3.5.3 correction of vision defects with the eyepiece graticules if you are going to use an eyepiece graticule you will need two adjustable graticules, e.G. Pl 10x/23 br. Foc., in order to correct different visio...

  • Page 69

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 69 4 operation 4.1 illumination and contrasting method 4.1.1 adjusting the transmitted light/bright-field according to kÖhler (1) general principle of operation the transmitted light/bright-field microscopy met...

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    Operation carl zeiss illumination and contrasting method axio scope.A1 70 m60-2-0007 e 05/08 • center the field diaphragm image with both centering screws ( 620h620h fig. 4-1/4) on the condenser carrier ( 621h621h fig. 4-1/c). Open the field diaphragm enough to make the edge of the diaphragm disappe...

  • Page 71

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 71 (4) adjusting the vertical stop on the condenser carrier • loosen the setscrew ( 625h625h fig. 4-2/1) on the vertical stop with the ball-head screwdriver sw 3. • focus the sample with the focusing drive. • c...

  • Page 72

    Operation carl zeiss illumination and contrasting method axio scope.A1 72 m60-2-0007 e 05/08 4.1.2 adjusting the transmitted light/dark-field according to kÖhler (1) general principle of operation uncolored biological samples, like bacteria or living cell cultures, are sometimes hard to see in the t...

  • Page 73

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 73 • replace the eyepiece into the tube. • lighter areas which might still be visible in the field of view are reduced when the dark-field condenser is adjusted correctly and delicately. You now have an approxi...

  • Page 74

    Operation carl zeiss illumination and contrasting method axio scope.A1 74 m60-2-0007 e 05/08 4.1.3 adjusting the transmitted light/phase-contrast (1) general principle of operation the phase-contrast method is ideal for examining thin uncolored samples, e.G. Culture cells. Generally the human eye ca...

  • Page 75

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 75 • if the overlap is not exact ( 632h632h fig. 4-5/a), the lighter annular diaphragm must be recentered with the aid of two hexagon screwdrivers sw 1.5 ( 633h633h fig. 4-4/1 and 4). Adjust the two centering s...

  • Page 76

    Operation carl zeiss illumination and contrasting method axio scope.A1 76 m60-2-0007 e 05/08 4.1.4 adjusting the transmitted light/differential interference contrast (dic) (1) general principle of operation the transmitted light/dic method allows for a high-contrast vivid display of transparent samp...

  • Page 77

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 77 1 dic slider 2 knurled screw 3 objective on the nosepiece 4 condenser with dic prism 5 compensator λ 6 analyzer module 7 reflector turret fig. 4-6 components for the transmitted light/dic method.

  • Page 78

    Operation carl zeiss illumination and contrasting method axio scope.A1 78 m60-2-0007 e 05/08 4.1.5 adjusting plasdic-contrast in transmitted light (1) general principle of operation plasdic can be used independently from the material of the specimen holder. The contrast method gives a relief-like im...

  • Page 79

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 79 4.1.6 adjusting transmitted light/polarization 4.1.6.1 how to show birefringence (1) application the transmitted light/polarization method is used for samples which change the polarization of the light. Such...

  • Page 80

    Operation carl zeiss illumination and contrasting method axio scope.A1 80 m60-2-0007 e 05/08 • bring the object to be examined into the field of view and turn it with the rotary stage. Normally, birefringent (anisotropic) objects will now show the same color and intensity variations as described abo...

  • Page 81

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 81 (3) adjusting the microscope • adjust the microscope as described in chapter 650h650h 4.1.1 (3) for transmitted light/bright-field. Make sure the eye distance is adjusted correctly on the binocular tube (see...

  • Page 82

    Operation carl zeiss illumination and contrasting method axio scope.A1 82 m60-2-0007 e 05/08 the phase difference results from the interference of the polarization of the sample and the polarization of the compensator λ. The larger phase difference occurs when the polarization direction of the sampl...

  • Page 83

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 83 4.1.7 adjusting the reflected light/bright-field according to kÖhler (1) application the reflected light/bright-field microscopy is the easiest and most commonly used optical microscopy method. It is used to...

  • Page 84

    Operation carl zeiss illumination and contrasting method axio scope.A1 84 m60-2-0007 e 05/08 • adjust the knurled knob on the field diaphragm ( 663h663h fig. 4-11/4) so that the field diaphragm becomes visible in the field of view ( 664h664h fig. 4-11/a). • readjust the focus on the edge of the fiel...

  • Page 85

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 85 1 centering screw of the aperture diaphragm 2 knurled button of the aperture diaphragm 3 centering screw of the field diaphragm 4 knurled button of the field diaphragm 5 focusing drive 6 nosepiece fig. 4-11 ...

  • Page 86

    Operation carl zeiss illumination and contrasting method axio scope.A1 86 m60-2-0007 e 05/08 4.1.8 adjusting the reflected light/dark-field (1) application the reflected light/dark-field method is applied when samples are examined which do not have areas with different reflectivity (ideal bright-fie...

  • Page 87

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 87 4.1.9 adjusting reflected light dic and reflected light c-dic (1) application the reflected light dic and the reflected light c-dic methods (dic = differential interference contrast; c- dic = differential in...

  • Page 88

    Operation carl zeiss illumination and contrasting method axio scope.A1 88 m60-2-0007 e 05/08 4.1.10 adjusting reflected light tic (1) application the reflected light tic method (micro-interferometry; tic = total interference contrast in the circular polarized light) is used in imaging and measuring ...

  • Page 89

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 89 the values a and b (see 684h684h fig. 4-14) are determined with the aid of the eyepiece graticule micrometer or with the micrometer eyepiece. If you work in the white light (without interference filter), you...

  • Page 90

    Operation carl zeiss illumination and contrasting method axio scope.A1 90 m60-2-0007 e 05/08 attention: • if the step and its surrounding are made from different materials, the phase jumps characteristic for the material have to be considered. For all non-conducting materials the phase jump is 180°,...

  • Page 91

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 91 4.1.11 adjusting reflected light polarization – proof of bireflexion and reflexion pleochroism (1) application the reflected light polarization is a contrasting method suited for cut surfaces of mineral ore,...

  • Page 92

    Operation carl zeiss illumination and contrasting method axio scope.A1 92 m60-2-0007 e 05/08 a sample is bireflective when the sample details show differences in brightness and color which change when the stage rotates. For samples with low bireflexion we advise using the analyzer with lambda plate,...

  • Page 93

    Operation axio scope.A1 illumination and contrasting method carl zeiss m60-2-0007 e 05/08 93 4.1.12 adjusting reflected light fluorescence (1) general principle of operation the reflected light fluorescence method is used to show fluorescent substances in typical fluorescence colors in high contrast...

  • Page 94

    Operation carl zeiss illumination and contrasting method axio scope.A1 94 m60-2-0007 e 05/08 • switch on the lamp hbo 100 or hbo 50 ( 691h691h fig. 4-16/1) and let it warm up to operational temperature for about 15 min. • on the reflector turret/slider ( 692h692h fig. 4-16/6), choose the reflector m...

  • Page 95

    Operation axio scope.A1 operating and functional elements of optional components carl zeiss m60-2-0007 e 05/08 95 4.2 operating and functional elements of optional components 4.2.1 lower stand part for led transmitted light illumination 1 led illumination (mounted on condenser carrier) 2 cord guide ...

  • Page 96

    Operation carl zeiss operating and functional elements of optional components axio scope.A1 96 m60-2-0007 e 05/08 4.2.2 lower stand part with hal 50 illumination 1 field diaphragm 2 filter wheel 6-fold (both sides) 3 focusing drive – fine adjustment (both sides) 4 focusing drive – rough adjustment (...

  • Page 97

    Operation axio scope.A1 operating and functional elements of optional components carl zeiss m60-2-0007 e 05/08 97 4.2.3 lower stand part for hal 100 illumination 1 field diaphragm 2 filter wheel 6-fold (both sides) 3 focusing drive – fine adjustment (both sides) 4 focusing drive – rough adjustment (...

  • Page 98

    Operation carl zeiss operating and functional elements of optional components axio scope.A1 98 m60-2-0007 e 05/08 4.2.4 stand column vario 380 mm or 560 mm with gear box 1 hand wheel for vertical adjustment of gear box of stand column, 2 gear box vario ready for upper stand part 3 focusing drive – r...

  • Page 99

    Operation axio scope.A1 operating and functional elements of optional components carl zeiss m60-2-0007 e 05/08 99 4.2.5 upper stand part transmitted light 1 nosepiece 6x bright-field 2 reflector insert (exchangeable) 4.2.6 upper stand part fl/hbo 1 field diaphragm (centerable) 2 port for adjustment ...

  • Page 100

    Operation carl zeiss operating and functional elements of optional components axio scope.A1 100 m60-2-0007 e 05/08 4.2.7 upper stand part fl-led 1 reflector turret 4-fold (fixed) 2 control wheel for illumination intensity and on/off switch of led illumination 3 pilot lamp led illumination on/off 4 n...

  • Page 101

    Operation axio scope.A1 operating and functional elements of optional components carl zeiss m60-2-0007 e 05/08 101 4.2.8 upper stand part hal 100/hbo 1 field diaphragm (centerable) 2 aperture diaphragm (centerable) 3 compartment for filter slider a 4 connecting piece for illumination unit 5 diffusin...

  • Page 102

    Operation carl zeiss operating and functional elements of optional components axio scope.A1 102 m60-2-0007 e 05/08 4.2.10 sideport iscp t60n left the sideport iscp t60n left is equipped with two switch positions which can be activated alternately with the slider. Push the slider to the right hand po...

  • Page 103

    Operation axio scope.A1 operating and functional elements of optional components carl zeiss m60-2-0007 e 05/08 103 4.2.11 reflector slider 2-fold the reflector slider 2-fold is equipped with two individually loadable reflector positions p&c which you can slide into the beam path. 4.2.12 reflector tu...

  • Page 104

    Operation carl zeiss operating and functional elements of optional components axio scope.A1 104 m60-2-0007 e 05/08 condenser 0.9/1.25 h the condenser 0.9/1.25 h ( 700h700h fig. 4-29/1) with aperture diaphragm ( 701h701h fig. 4-29/2) is equipped to your choice with a modulator disk ( 702h702h fig. 4-...

  • Page 105

    Operation axio scope.A1 operating and functional elements of optional components carl zeiss m60-2-0007 e 05/08 105 binocular ergo tube / ergo photo tube 20°/23 the ergo tubes are designed for the field of view 23. The view angle is 20°. The ergo tubes allow for a progressive vertical adjustment with...

  • Page 106

    Operation carl zeiss operating and functional elements of optional components axio scope.A1 106 m60-2-0007 e 05/08 binocular photo tube (100/100) the photo port ( 710h710h fig. 4-32/1) of the binocular tube may serve for mounting a reflex camera, a microscope camera or a video camera with the aid of...

  • Page 107

    Operation axio scope.A1 operating and functional elements of optional components carl zeiss m60-2-0007 e 05/08 107 mechanical stage 75x50 − mechanical stage ( 717h717h fig. 4-34/2) with object holder ( 718h718h fig. 4-34/1) for holding, positioning and fixating the samples. − gear knobs for x ( 719h...

  • Page 108

    Operation carl zeiss operating and functional elements of optional components axio scope.A1 108 m60-2-0007 e 05/08 diaphragm slider 14x40 mm for aperture and field diaphragm − you will need one diaphragm slider 14x40 mm each ( 723h723h fig. 4-36/4, inserted in the upper stand part as field diaphragm...

  • Page 109

    Maintenance, changing of fuses and service axio scope.A1 maintenance carl zeiss m60-2-0007 e 05/08 109 5 maintenance, changing of fuses and service 5.1 maintenance maintenance of the axio scope.A1 is limited to the following activities: • turn the instrument off after each use and cover it with the ...

  • Page 110

    Maintenance, changing of fuses and service carl zeiss technical service axio scope.A1 110 m60-2-0007 e 05/08 5.2 technical service 5.2.1 check-up • be sure to adhere to the required voltage (e.G. On the transformer hbo 100 w). • check the power cable and the power plug for damages. • as soon as you ...

  • Page 111

    Maintenance, changing of fuses and service axio scope.A1 trouble shooting carl zeiss m60-2-0007 e 05/08 111 5.3 trouble shooting problem cause fault clearance push rod/gear knob vis/fot on the photo tube is not in correct functional position (interposition). Bring the push rod/gear knob vis/fot on t...

  • Page 112

    Maintenance, changing of fuses and service carl zeiss trouble shooting axio scope.A1 112 m60-2-0007 e 05/08 problem cause fault clearance condenser is not adjusted properly. Re-adjust the condenser, see p. 733h733h 69 ff. Asymmetric image sharpness, e.G. One side is sharp, one is side blurred. Nosep...

  • Page 113

    Maintenance, changing of fuses and service axio scope.A1 trouble shooting carl zeiss m60-2-0007 e 05/08 113 problem cause fault clearance halogen lamp 12 v, 50 w does not glow although the switch in the on-position. Power plug is not plugged into the outlet. Insert the plug into the outlet. Make sur...

  • Page 114

    Maintenance, changing of fuses and service carl zeiss service axio scope.A1 114 m60-2-0007 e 05/08 5.4 service service work on the mechanical, optical and electronic parts inside the instrument and on the electrical equipment of the microscopes axio scope.A1 may only be performed by the carl zeiss s...

  • Page 115

    Annex axio scope.A1 list of abbreviations carl zeiss m60-2-0007 e 05/08 115 6 annex 6.1 list of abbreviations ac alternating current acr automatic component recognition a-plan achromatic objectives with improved flatness of field (ics optical products) br. Suitable for spectacle wearers ccd charge-c...

  • Page 116

    Annex carl zeiss list of abbreviations axio scope.A1 116 m60-2-0007 e 05/08 r right-hand (gear knob on the right-hand side of the mechanical stage) slr single lens reflex sw wrench size t slow (type of fuse) tic total interference contrast in circular polarized light ul underwriter laboratories uv u...

  • Page 117

    Annex axio scope.A1 subject index carl zeiss m60-2-0007 e 05/08 117 6.2 subject index page a achromatic illumination adapter ............................................................................................................17 adapter ...........................................................

  • Page 118

    Annex carl zeiss subject index axio scope.A1 118 m60-2-0007 e 05/08 f field diaphragm.......................................................................................................19, 69, 75, 94, 96, 97 filter......................................................................................

  • Page 119

    Annex axio scope.A1 subject index carl zeiss m60-2-0007 e 05/08 119 n nosepiece....................................................................................................................19, 33, 69, 83, 104 o object holder.........................................................................

  • Page 120

    Annex carl zeiss subject index axio scope.A1 120 m60-2-0007 e 05/08 transmitted light .....................................................................................................69, 72, 74, 76, 78, 79 transmitted light lamp.......................................................................

  • Page 121

    Annex axio scope.A1 property rights carl zeiss m60-2-0007 e 05/08 121 6.3 property rights the instruments, their components and the methods described in this manual are protected by the following patents: − us 6154282 − us 6392796 − us 5015082 − us 6595661 − de 4322015 − us 5488512 − us 5817256 − us...

  • Page 123: Click Below To Find More

    Click below to find more mipaper at www.Lcis.Com.Tw mipaper at www.Lcis.Com.Tw.