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Zeiss Axiolab A Operating Manual

Manual is about: Reflected-Light

Summary of Axiolab A

  • Page 1

    Axiolab a reflected-light microscope operating manual.

  • Page 2

    Carl zeiss axiolab a ii b 40-015 e 06/99 knowledge of this instruction manual is necessary for device operation. Please get familiar with its contents and especially the precautions for safe device operation. Changes due to further technical development are reserved; this manual is not covered by an...

  • Page 3

    Axiolab a carl zeiss b 40-015 e 06/99 iii contents page introduction ................................................................................................................. I title page ...........................................................................................................

  • Page 4

    Carl zeiss axiolab a iv b 40-015 e 06/99 page 3 operation ................................................................................................................... 3-1 3.1 switch on the instrument ................................................................................................

  • Page 5

    Axiolab a carl zeiss b 40-015 e 06/99 v list of illustrations page fig. 1-1 axiolab a main modules .................................................................................................. 1-3 fig. 1-2 optical design of the axiolab a with transmitted-light equipment............................

  • Page 6

    Carl zeiss axiolab a vi b 40-015 e 06/99 fig. 4-1 changing the fuses ......................................................................................................... 4-4 fig. 4-2 changing the 6 v, 30 w reflected-light halogen illuminator ............................................. 4-5 fig...

  • Page 7

    Axiolab a carl zeiss b 40-015 e 06/99 vii notes on instrument safety the axiolab a reflected-light microscope was designed, produced and tested in compliance with din 61010-1 (iec 1010-1), safety requirements for electrical measuring, control and laboratory instruments, and meets the requirements of...

  • Page 8

    Carl zeiss axiolab a viii b 40-015 e 06/99 the axiolab a microscope, including original accessories, may only be used for the microscope techniques described in this manual. Particular attention must be paid to the following warning notes: the manufacturer cannot assume any liability for any other a...

  • Page 9

    Axiolab a carl zeiss b 40-015 e 06/99 ix dust and dirt can impair the performance of the instrument. Therefore, the instrument must be protected against these influences as far as possible, and covered with the dust cover if it is not used for longer periods of time. Always check whether the instrum...

  • Page 10

    Carl zeiss axiolab a x b 40-015 e 06/99 overall view of the axiolab a reflected-light microscope.

  • Page 11: Description

    Axiolab a carl zeiss b 40-015 e 06/99 1-1 description contents 1 description .................................................................................................................1-2 1.1 name; intended application,..............................................................................

  • Page 12

    Carl zeiss axiolab a 1-2 06/99 b 40-015 e 1 description 1.1 name; intended application, manufacturer's name: upright reflected-light microscope for routine examinations within the product family of upright reflected-light microscopes, the axiolab a fits in as follows: • routine microscopes • researc...

  • Page 13

    Axiolab a carl zeiss b 40-015 e 06/99 1-3 fig. 1-1 axiolab a main modules the specimen stage can be designed - as fixed stage (1-1/3) with stage clamps, - as glide stage (1-1/5) with stage clamps, 25 × 25 mm travel range, or - as mechanical stage (1-1/4) 75 × 35 r/a with ceramic surface and short dr...

  • Page 14

    Carl zeiss axiolab a 1-4 06/99 b 40-015 e the reflected-light halogen illuminator (1-1/10) is equipped with a 6v 30 w halogen lamp as a standard. Its luminous intensity (lamp voltage) is continuously variable via the relevant control. The correct color temperature for color photography (3200 k) is a...

  • Page 15

    Axiolab a carl zeiss b 40-015 e 06/99 1-5 the optical performance of the axiolab a can be ideally matched to the relevant requirements by using various objectives which are inserted into a 5-position nosepiece (1-1/6). The camera port (1-1/9) attached to the binocular reflected-light tube (1-1/8) is...

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    Carl zeiss axiolab a 1-6 06/99 b 40-015 e fig. 1-3 axiolab a microscope configurations.

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    Axiolab a carl zeiss b 40-015 e 06/99 1-7 axiolab a microscope configurations cat. No. Fixed stage glide stage mechani- cal stage 1 configurations 1 microscope axiolab a with fixed mechanical stage 490960-9804-000 • 2 microscope axiolab a with glide stage 490962-9804-000 • 3 microscope axiolab a wit...

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    Carl zeiss axiolab a 1-8 06/99 b 40-015 e fig. 1-4 axiolab a accessories (1 of 2).

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    Axiolab a carl zeiss b 40-015 e 06/99 1-9 axiolab a accessories cat. No. Fixed stage glide stage mechani- cal stage stages 1.1 glide stage with stage carrier 453509-0000-000 ! ! ! ! 1.2 mechanical stage 75 × 35 r/a with ceramic surface and short drive, plus: 453517-9901-000 + + 1.2.1 specimen holder...

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    Carl zeiss axiolab a 1-10 06/99 b 40-015 e fig. 1-4 axiolab a accessories (2 of 2).

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    Axiolab a carl zeiss b 40-015 e 06/99 1-11 axiolab a accessories cat. No. Fixed stage glide stage mechani- cal stage 7 retrofit package for transmitted-light illumination (can only be fitted by service personnel), consisting of: ! ! ! ! ! ! ! ! 7.1 - mechanical stage 75 × 35 r/a with ceramic surface...

  • Page 22

    Carl zeiss axiolab a 1-12 06/99 b 40-015 e 1.4 function elements (see fig. 1-5, after this table) item designation purpose/description 1 stand module base of the axiolab a microscope 2 6 v, 30 w reflected-light halogen illuminator reflected-light illuminator with 6 v, 30 w halogen lamp 3 reflected-l...

  • Page 23

    Axiolab a carl zeiss b 40-015 e 06/99 1-13 item designation purpose/description 14 fuse holder holder for two fuse inserts 15 fuse insert t 0.8 a; 250 v; 5 × 20 mm 17 mains cable to apply the supply voltage 18 pushrod pushrod to change the beam path between - observation through the binocular tube (...

  • Page 24

    Carl zeiss axiolab a 1-14 06/99 b 40-015 e item designation purpose/description 27 ”luminous intensity” control continuous control of the illuminance of the light source by changing the lamp voltage in the range from > 1.5...6 v. At 6 v (right stop) the 3200 k color temperature for color photography...

  • Page 25

    Axiolab a carl zeiss b 40-015 e 06/99 1-15 1 stand 2 6 v, 30 w halogen illuminator 3 reflected-light illuminator h 4 camera port 5 binocular tube 6 eyepiece, fixed 7 focusing eyepiece 8 dust protection slider 9 5-position objective nosepiece 10 specimen stage, fixed 11 coaxial coarse and fine drive ...

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    Carl zeiss axiolab a 1-16 06/99 b 40-015 e fig. 1-5 axiolab a function elements (1 of 2).

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    Axiolab a carl zeiss b 40-015 e 06/99 1-17 fig. 1-5 axiolab a function elements (2 of 2) 1 1 2.

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    Carl zeiss axiolab a 1-18 06/99 b 40-015 e 1.5 objectives the objectives are the optical centerpiece of the microscope. The following is an example of how objectives can be labelled: epiplan 10 × /0.20 ∞ /- where 10 × : objective magnification, with a defined color ring on the objective being alloca...

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    Axiolab a carl zeiss b 40-015 e 06/99 1-19 the following objectives are available for the axiolab a: microscopy technique objective magnification/ num. Aperture free working distance in mm cover slip thickness d in mm cat. No. Reflected-light brightfield epiplan epiplan 5 × /0.13 19.8 - 442920-0000-...

  • Page 30

    Carl zeiss axiolab a 1-20 06/99 b 40-015 e 1.7 stage micrometers and eyepiece reticles measuring and counting using the microscope requires stage micrometers and eyepiece reticles, a small selection of which is listed below: illustration description, technical data cat. No. Stage micrometer, positiv...

  • Page 31

    Axiolab a carl zeiss b 40-015 e 06/99 1-21 net micrometer 12.5 ×××× 12.5 / 5 ; 10, d = 26 mm area 12.5 × 12.5 mm, divided in fields of 10 × 10 474068-0000- 000 photo reticle mc 2.5 ×××× / d = 26 mm for 35 mm photography with an additional magnification of 2.5 ×××× or for large-format photography wit...

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    Carl zeiss axiolab a 1-22 06/99 b 40-015 e 1.8 technical data dimensions and weight dimensions (width × depth × height) ......................................................................180 × 400 × 430 mm footprint (recommended with mat)..............................................................

  • Page 33

    Axiolab a carl zeiss b 40-015 e 06/99 1-23 mechanical data of specimen stages stage models............................................................................. Fixed stage/glide stage/mechanical stage maximum specimen height: stage at bottom stop (not possible for mechanical stage).............

  • Page 34

    Carl zeiss axiolab a 1-24 06/99 b 40-015 e.

  • Page 35: Start-Up

    Axiolab a carl zeiss b 40-015 e 06/99 2-1 start-up contents 2 start-up .........................................................................................................................2-3 2.1 unpacking the instrument...............................................................................

  • Page 36

    Carl zeiss axiolab a 2-2 b 40-015 e 06/99.

  • Page 37

    Axiolab a carl zeiss b 40-015 e 06/99 2-3 2 start-up the three versions of the axiolab a reflected-light microscope, including accessories, are supplied in standard packaging. We would recommend you to keep the transport cases for storage or return of the instrument to the manufacturer. 2.1 unpackin...

  • Page 38

    Carl zeiss axiolab a 2-4 b 40-015 e 06/99 2.3 insertion of eyepieces • remove both protection caps from the binocular tube. • insert the fixed eyepiece, e.G. E-pl 10 × /20 br., in one tube and the focusing eyepiece e-pl 10 × /20 br. Foc. In the other tube. ☞ the focusing eyepiece is used to compensa...

  • Page 39

    Axiolab a carl zeiss b 40-015 e 06/99 2-5 2.3.2 compensation of ametropia when eyepiece reticles are used the correct use of an eyepiece reticle requires two focusing eyepieces, e.G. Pl 10 × /20 br. Foc., to enable compensation of ametropia. • use the eyelens of the focusing eyepiece to focus on the...

  • Page 40

    Carl zeiss axiolab a 2-6 b 40-015 e 06/99 2.5 attachment of reflected-light halogen illuminator • attach dovetail guide (2-4/3) of 6 v, 30 w halogen lamp (2-4/4) to the microscope stand and tighten hexagonal screw (2-4/2) using the sw 3 screwdriver (2-4/1). ☞ the halogen lamp must be mounted to the ...

  • Page 41

    Axiolab a carl zeiss b 40-015 e 06/99 2-7 2.6.1 switch on the transmitted-light halogen illuminator ☞ normally, the position of the a/d illumination converter (1-5/13 or 2-7/4) is of no importance with the axiolab a. Only if the transmitted-light illumination is a standard feature or has been subseq...

  • Page 42

    Carl zeiss axiolab a 2-8 b 40-015 e 06/99 2.8 set the luminous-field diaphragm • the pushrod (2-6/1) for the luminous-field diaphragm has two positions: − pushed in: the full field of view is visible without restriction. − pulled out: the visible field of view is reduced to approx. 1/3 of the overal...

  • Page 43: Operation

    Axiolab a carl zeiss b 40-015 e 06/99 3-1 operation contents 3 operation ......................................................................................................................3-3 3.1 switch on the instrument................................................................................

  • Page 44

    Carl zeiss axiolab a 3-2 b 40-015 e 06/99.

  • Page 45

    Axiolab a carl zeiss b 40-015 e 06/99 3-3 3 operation 3.1 switch on the instrument • switch on the instrument via the on/off switch (3-1/6). − the green line control lamp in the switch (3- 1/6) must light up. − the halogen lamp 6 v, 30 w in the reflected-light illuminator (3-1/2) must light up. ☞ th...

  • Page 46

    Carl zeiss axiolab a 3-4 b 40-015 e 06/99 3.2 illumination and contrasting techniques the description of the illumination and contrasting techniques is based on the following microscope settings: • the axiolab a microscope is ready for operation as described in chapter 2 and switched on as described...

  • Page 47

    Axiolab a carl zeiss b 40-015 e 06/99 3-5 3.2.2 setting of reflected-light polarization the axiolab a microscope is ready for operation as described in chapter 2, and switched on as described in section 3.1. • pull out the plane glass pushrod (3-3/1). • pull out the dust protection sliders (3-3/2+6)...

  • Page 48

    Carl zeiss axiolab a 3-6 b 40-015 e 06/99 (1) centering of objectives (only for axiolab pol catalogue no. 450910-0000-000) fig. 3-4 centering of objectives when the pol stage (3-4/3) and uncentered objectives are turned, object details above the center of the eyepiece cross migrate to circular paths...

  • Page 49

    Axiolab a carl zeiss b 40-015 e 06/99 3-7 (2) transmitted-light polarization - detection of birefringence this technique is used for the examination of transparent, birefringent objects. Birefringence can be recognized, with crossed polarizer and analyzer, by the otherwise dark field of view being b...

  • Page 50

    Carl zeiss axiolab a 3-8 b 40-015 e 06/99 (3) transmitted-light polarization - determination of the n γγγγ ' vibration direction and determination of the path difference fig. 3-6 determine the n γγγγ ' vibration direction using the example of an artificial fiber application the position of the two d...

  • Page 51

    Axiolab a carl zeiss b 40-015 e 06/99 3-9 conclusions the n γ -direction of the λ -compensator is ne-sw oriented. The environment of the fiber exhibits a dark, first-order red (path difference is one λ ; approx. 550 nm). The fiber itself appears greenish blue (path difference approx. 700 nm). The hi...

  • Page 52

    Carl zeiss axiolab a 3-10 b 40-015 e 06/99 (4) transmitted-light polarization - determination of the optical character of crystals application the optical character of transparent and weakly absorbing crystals must be determined for the diagnosis of crystals. The determination is made in conoscopic ...

  • Page 53

    Axiolab a carl zeiss b 40-015 e 06/99 3-11 uniaxial crystals if the optical axis of a uniaxial crystal is oriented parallel to the observation direction, a dark cross which can be surrounded by concentric interference rings (depending on birefringence and specimen thickness) becomes visible in the c...

  • Page 54

    Carl zeiss axiolab a 3-12 b 40-015 e 06/99 (5) reflected-light polarization - detection of bireflection and reflection pleochroism application polished sections of ores, coals, ceramic products, certain metals and metal alloys display a different reflection behavior, depending on the orientation of ...

  • Page 55

    Axiolab a carl zeiss b 40-015 e 06/99 3-13 3.2.4 setting of epi-fluorescence the fluorescence technique allows fluorescent structures to be visualized with high contrast in a typical fluorescence color. The examined material can be autofluorescent or may have been mixed with fluorescent substances (...

  • Page 56

    Carl zeiss axiolab a 3-14 b 40-015 e 06/99 3.2.5 setting of transmitted-light brightfield (kÖhler illumination) make the microscope ready for operation as described in chapter 2 and section 3.1. • switch on the illumination, place a high- contrast specimen on the stage, and focus using the 10 × obje...

  • Page 57

    Axiolab a carl zeiss b 40-015 e 06/99 3-15 3.3 attachment of microscope stages and specimen holders the axiolab a microscope is available with three different stages: - fixed stage (3-11/1), - gliding stage (3-11/2) or - mechanical stage (3-11/3). The retaining clips (3-11/4) are used to hold specim...

  • Page 58

    Carl zeiss axiolab a 3-16 b 40-015 e 06/99 3.3.1 attachment of pol rotary stage fig. 3-13 pol rotary stage- setting of specimen mount and stop the stage clips (3-13/3) are used to mount the specimen on the pol rotary stage (3-13/8). They are inserted into two of the three drilled holes (3-13/9) and,...

  • Page 59

    Axiolab a carl zeiss b 40-015 e 06/99 3-17 3.4 use of polished section attachment the polished section attachment from the line of accessories provides another possibility of holding objects for examinations in incident light. It has been intended for use with the gliding stage. ☞ basically, the pol...

  • Page 60

    Carl zeiss axiolab a 3-18 b 40-015 e 06/99 3.5 photomicrography and videomicroscopy the axiolab a microscope can be changed from observation to photomicrography or videomicroscopy via a pushrod (3-16/7 or 3-18/8) (pushrod pulled out for photomicrography or videomicroscopy). Since this is a 100% chan...

  • Page 61

    Axiolab a carl zeiss b 40-015 e 06/99 3-19 3.5.1 attachment of photomicrography equipment (1) attachment of slr camera, e.G. Contax 167 mt • screw t2 adapter for the contax bayonet (3- 16/3) on the 2.5 × connector for t2 (3-16/4) (456005-0000-000). • attach the camera housing (3-16/2) and the cable ...

  • Page 62

    Carl zeiss axiolab a 3-20 b 40-015 e 06/99 various t2 adapters for slr cameras are listed below: t2 adapters to slr cameras cat. No. T2 adapter for contax (contax bayonet) 416010-0000-000 t2 adapter for olympus om (om bayonet) 416002-0000-000 t2 adapter for minolta (sr bayonet) 416003-0000-000 t2 ad...

  • Page 63

    Axiolab a carl zeiss b 40-015 e 06/99 3-21 (2) attachment of mc 80 dx microscope camera (35 mm film cassette) • insert adapter 60 for microscope camera (3- 17/5) (456006-0000-000) in camera tube (3- 17/6) and fix it using three hexagonal screws (3-17/7). • insert projection lens p 2.5 × (3-17/4) in ...

  • Page 64

    Carl zeiss axiolab a 3-22 b 40-015 e 06/99 3.5.2 attachment of adapters for video cameras the following video adapters with 60 mm interface permit the attachment of one-chip b/w and color ccd cameras and 3-chip color ccd cameras to the camera port of the axiolab a . Tube adapter cameras 456105 45610...

  • Page 65

    Axiolab a carl zeiss b 40-015 e 06/99 3-23 attachment of video cameras: • loosen three hexagonal screws and remove dust cover from the axiolab a camera tube. • screw video adapter or video zoom adapter with c-mount thread into the video camera. Insert video adapter or video zoom adapter in eng 2/3“ ...

  • Page 66

    Carl zeiss axiolab a 3-24 b 40-015 e 06/99 3.6 insertion of 8 ×××× drawing eyepiece the 8 × drawing eyepiece (444126-0000-000) is an accessory for microscopic drawing and can only be used in combination with the binocular reflected-light tube 30°/20 h 1 on the axiolab a. It contains a beam splitter ...

  • Page 67

    Axiolab a carl zeiss b 40-015 e 06/99 4-1 care, troubleshooting and service contents 4 care, troubleshooting and service....................................................................4-3 4.1 maintenance of the instrument .............................................................................

  • Page 68

    Carl zeiss axiolab a 4-2 b 40-015 e 06/99.

  • Page 69

    Axiolab a carl zeiss b 40-015 e 06/99 4-3 4 care, troubleshooting and service 4.1 maintenance of the instrument maintenance of the axiolab a is limited to the following operations: • cover the instrument with the dust cover after every use. • do not set up the instrument in a damp room, i.E. Max. Hu...

  • Page 70

    Carl zeiss axiolab a 4-4 b 40-015 e 06/99 4.2 troubleshooting troubleshooting on the axiolab a microscope is limited to only a few actions: • checking the line cable • checking the set instrument voltage and changing the fuses • checking the illumination and changing the lamp − defective fuse(s) → c...

  • Page 71

    Axiolab a carl zeiss b 40-015 e 06/99 4-5 (2) changing the 6 v, 30 w reflected-light halogen lamp the following procedure is required to change the lamp of the 6 v, 30 w reflected-light halogen illuminator: • switch off the lamp supply; if required, allow the illuminator to cool down for approx. 15 ...

  • Page 72

    Carl zeiss axiolab a 4-6 b 40-015 e 06/99 (3) changing the 6 v, 30 w transmitted- light halogen lamp the following procedure is required to change the lamp of the integrated 6 v, 30 w illuminator: • switch off the extern lamp supply; if required, allow the illuminator to cool down for approx. 15 min...

  • Page 73

    Axiolab a carl zeiss b 40-015 e 06/99 a-1 annex - list of abbreviation ..................................................................................................................... A-3 - certification in accordance with din en iso 9001 / din en 46001 ............................................

  • Page 74

    Carl zeiss axiolab a a-2 b 40-015 e 06/99.

  • Page 75

    Axiolab a carl zeiss b 40-015 e 06/99 a-3 list of abbreviations a incident light, analyzer ac alternating current br. Eyepieces suitable for spectacle wearers ccd charge-coupled device csa canadian standards association d transmitted light, filter attenuation in % din german standards association dm...

  • Page 76

    Carl zeiss axiolab a a-4 b 40-015 e 06/99 t slow-blow fuse type tv television t2-adapter standard adapter for 35 mm cameras.