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Zeiss Axiovert 100 Operating Manual

Manual is about: 3D Microscopy

Summary of Axiovert 100

  • Page 1

    3d microscopy for axiovert 100/135/135 m operating manual.

  • Page 2

    Copyright 3d microscopy 2 b 40-022-4 e 07/98 knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specificat...

  • Page 3: Contents

    3d microscopy contents b 40-022-4 e 07/98 3 contents 1 notes on safety ................................................................................................... 4 1.1 general.......................................................................................................................

  • Page 4: Notes On Safety

    Notes on safety 3d microscopy 4 b 40-022-4 e 07/98 1 notes on safety 1.1 general please make yourself familiar with the contents of these operating instructions before attaching and starting up the components for 3d microscopy. Additional information is available from our maintenance service or from...

  • Page 5

    3d microscopy notes on unpacking, transport and storage b 40-022-4 e 07/98 5 1.3 notes on unpacking, transport and storage the components for 3d microscopy must be transported in their original packaging. It is recommended to use the original packaging also for storage. The transport and storage tem...

  • Page 6: General

    General application 3d microscopy 6 b 40-022-4 e 07/98 2 general 2.1 application 3d microscopy permits the stereoscopic viewing of microscope specimens and objects up to highest magnifications using all objectives eyepiece and monitor observation in reflected and transmitted light on upright and inv...

  • Page 7

    3d microscopy function eypiece version b 40-022-4 e 07/98 7 2.2 function the dynamic technique on which 3d microscopy is based can be used with any light microscope with an accessible aperture diaphragm. The technique can be used with upright or inverted reflected-light and transmitted-light microsc...

  • Page 8

    Function monitor version 3d microscopy 8 b 40-022-4 e 07/98 1 eyepiece with 3d-shutter 2 3d-control dsa-3 3 depolarizer 4 3d illumination shutter 5 condenser 6 object plane 7 objective eyepiece version in a transmitted-light microscope the depolarizer is required for minimization of disturbances by ...

  • Page 9

    3d microscopy function monitor version b 40-022-4 e 07/98 9 1 monitor shutter 2 glasses 3 control unit for monitor shutter 4 mcu ii image memory 5 3d illumination shutter 6 objective 7 object plane 8 camera monitor version in a reflected-light microscope 1 monitor shutter 2 polarizing glasses 3 came...

  • Page 10

    Function combined version 3d microscopy 10 b 40-022-4 e 07/98 1 monitor shutter 2 polarizing glasses 3 camera 4 control unit for monitor shutter 5 mcu ii image memory 6 3d-control dsa-3 7 3d illumination shutter 8 depolarizer 9 object plane 10 eyepiece with 3d shutter combined version in a transmitt...

  • Page 11

    3d microscopy maintenance and care b 40-022-4 e 07/98 11 2.3 maintenance and care 2.3.1 general never expose the components for 3d microscopy to inadmissible climatic conditions (high humidity and temperature) for longer periods of time. The components must be protected from dust and humidity and co...

  • Page 12

    Troubleshooting 3d microscopy 12 b 40-022-4 e 07/98 2.4 troubleshooting the monitor displays colored fringing or several out-of-focus images: switch off mcu ii; switch it on again after 10 seconds no 3d image is obtained: monitor shutter does not function; check switch for monitor shutter on the con...

  • Page 13: Technical Data

    3d microscopy technical data b 40-022-4 e 07/98 13 3 technical data parameters description ambient conditions operation storage transport temperature + 10°c ... + 35°c 0°c ... + 35°c 20°c ... + 50°c humidity max. 75% at 35°c condensation water not admissible condensation water not admissible conditi...

  • Page 14

    3d microscopy contents axiovert 100/135 14 b 40-022-4 e 09/98.

  • Page 15: 3D Microscopy For

    3d microscopy axiovert 100/135/135 m contents b 40-022-4 e 09/98 15 3d microscopy for axiovert 100/135/135 m contents 1 components for 3d microscopy overview .................................................. 16 2 eyepiece version.........................................................................

  • Page 16: Components For 3D Microscopy

    3d microscopy overview axiovert 100/135/135 m 16 b 40-022-4 e 09/98 1 components for 3d microscopy overview fig. 1 overview.

  • Page 17

    3d microscopy axiovert 100/135/135 m overview b 40-022-4 e 09/98 17 axiovert 100/135/135 m eyepiece version monitor version eyepiece and monitor version 4 3d illumination shutter 3d illumination shutter 3d illumination shutter 2 eyepiece pl 10x/25 with 3d shutter eyepiece pl 10x/25 with 3d shutter 5...

  • Page 18: Eyepiece Version

    3d microscopy eyepiece version axiovert 100/135/135 m 18 b 40-022-4 e 09/98 2 eyepiece version 2.1 converting the microscope 1 eyepieces with 3d shutter 2 3d-control dsa-3 3 line connection 4 3d illumination shutter fig. 2 connections for eyepiece version 1 fuse compartment 2 voltage selector 3 hold...

  • Page 19

    3d microscopy axiovert 100/135/135 m eyepiece version b 40-022-4 e 09/98 19 1 stop screw (hexagonal, sw 2, varnish-coated) 2 ld condenser 3 fixation screw (hexagonal, sw 2) 4 recess 5 3d illumination shutter fig. 4 insertion of 3d illumination shutter 1 depolarizer 2 recess (analyzer opening) fig. 5...

  • Page 20

    3d microscopy eyepiece version axiovert 100/135/135 m 20 b 40-022-4 e 09/98 2.2 3d-control dsa-3 control unit fig. 6 3d-control dsa-3 control unit, front 2.2.1 connections fig. 6 eyepiece connection sockets for eyepieces with 3d shutter illumination shutter connection socket for 3d illumination shut...

  • Page 21

    3d microscopy axiovert 100/135/135 m eyepiece version b 40-022-4 e 09/98 21 2.3 adjustment fig. 7 adjustment of 3d illumination shutter, pupil use cover slip as an object and focus on it using the 10x objective; after adjustment, check the setting using the objective of the highest magnification 2.3...

  • Page 22

    3d microscopy eyepiece version axiovert 100/135/135 m 22 b 40-022-4 e 09/98 2.3.2 check the contrast if colors or inhomogeneity are visible in the pupil, change the voltage settings for the 3d illumination shutter and the 3d shutter of the eyepieces on the potentiometers of the 3d- control das-3 unt...

  • Page 23

    3d microscopy axiovert 100/135/135 m eyepiece version b 40-022-4 e 09/98 23 2.3.3 check whether image is upright fig. 8 labels to mark the eyepieces and plugs set the rotary switch of the 3d-control das-3 to 3d vis set the toggle switch of the 3d-control das-3 to stereo view a familiar object; if th...

  • Page 24: Monitor Version

    3d microscopy eyepiece version axiovert 100/135/135 m 24 b 40-022-4 e 09/98 3 monitor version the following components from kappa are required for the monitor version: mcu ii control unit cf 15/4 mc-s camera cf 15/4 connecting cable to mcu ii line cable monitor (100 hz) with connecting cable and att...

  • Page 25

    3d microscopy axiovert 100/135/135 m monitor version b 40-022-4 e 09/98 25 3.1 conversion 1 monitor 2 bracket 3 monitor shutter fig. 10 attachment of monitor shutter 1 loop-through input for video printer/video recorder 2 socket for camera cable 3 socket for video printer/video recorder 4 output for...

  • Page 26

    3d microscopy monitor version axiovert 100/135/135 m 26 b 40-022-4 e 09/98 1 camera 2 adapter 3 phototube fig. 12 attachment of camera connect camera to phototube via instrument-specific video adapter or video zoom; the following adapters are recommended: video zoom adapter 0.4x 2x with c-mount tv a...

  • Page 27

    3d microscopy axiovert 100/135/135 m monitor version b 40-022-4 e 09/98 27 3.2 mcu ii control unit 1 line switch 2 menu change 3 menu display (lcd) 4 storage key for current image 5 arrow keys for parameter control in the menu fig. 13 mcu ii controls, front 3.2.1 controls fig. 13 lcd-display display...

  • Page 28

    3d microscopy monitor version axiovert 100/135/135 m 28 b 40-022-4 e 09/98 3.3 adjustment use cover slip as an object and focus on it using the10x objective; after adjustment, check the setting using the objective of the highest magnification check center position of the 3d illumination shutter in t...

  • Page 29

    3d microscopy axiovert 100/135/135 m monitor version b 40-022-4 e 09/98 29 3.4 3d video recordings 3d sequences can generally be recorded and played back using a video recorder. This requires an s-vhs recorder and an s-vhs video tape. Fig. 14 wiring diagram for video prints connecting the video reco...

  • Page 30: Eyepiece and

    3d microscopy eyepiece and monitor version axiovert 100/135/135 m 30 b 40-022-4 e 09/98 4 eyepiece and monitor version before connecting the components, first read the enclosed manual from kappa entitled "color tv camera for microscopy and 3d images"; chapter 5, "safety regulations", should be obser...

  • Page 31

    3d microscopy axiovert 100/135/135 m eypiece and monitor version b 40-022-4 e 09/98 31 4.2 observation this is a combination of the eyepiece and the monitor version. The image is 3-dimensional when you look through the eyepiece or on the monitor. 4.2.1 eyepiece observation set the beam path to obser...

  • Page 32

    3d microscopy eyepiece and monitor version axiovert 100/135/135 m 32 b 40-022-4 e 09/98.