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Zeiss Standard 25 ICS Operating Manual

Manual is about: Transmitted Light Microscope

Summary of Standard 25 ICS

  • Page 1

    Standard 25 ics transmitted light microscope operating manual.

  • Page 2

    Standard 25 ics ii b 40-029 e 01/98 knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specifications are ...

  • Page 3

    Standard 25 ics b 40-029 e 01/98 iii contents page introduction........................................................................................................................ I title page ..........................................................................................................

  • Page 4

    Standard 25 ics iv b 40-029 e 01/98 3 operation.......................................................................................................................... 3-3 3.1 switch on the instrument ....................................................................................................

  • Page 5

    Standard 25 ics b 40-029 e 01/98 v notes on instrument safety the standard 25 ics microscope was designed, produced and tested in compliance with din 61010-1 (iec 1010-1), safety requirements for electrical measuring, control and laboratory instruments, and meets the requirements of appendix i of di...

  • Page 6

    Standard 25 ics vi b 40-029 e 01/98 the standard 25 ics microscope, including its original accessories, may only be used for the microscope techniques described in this manual. Particular attention must be paid to the following warning notes: the standard 25 ics microscope is categorized as protecti...

  • Page 7

    Standard 25 ics b 40-029 e 01/98 vii the standard 25 ics microscope is an optical precision instrument which can be impaired in its performance or damaged when handled improperly. Notes on warranty: the manufacturer guarantees that the instrument has no material and production defects when delivered...

  • Page 8

    Standard 25 ics viii b 40-029 e 01/98 overall view of the standard 25 ics microscope with polarization equipment.

  • Page 9: Description

    Standard 25 ics b 40-029 e 01/98 1-1 description contents 1 description .................................................................................................................1-3 1.1 name and intended application.................................................................................

  • Page 10

    Standard 25 ics 1-2 b 40-029 e 01/98 fig. 1-1 microscope modules 1 eyepieces 5 condenser carrier with condenser 2 binocular tube 6 mechanical stage with specimen holder 3 stand 7 objective 4 coaxial coarse and fine drive 8 objective nosepiece.

  • Page 11

    Standard 25 ics b 40-029 e 01/98 1-3 1 description 1.1 name and intended application manufacturer's name: standard 25 ics microscope the standard 25 ics microscope is a universal microscope for the visualization of fine structures and forms in biology and medicine. These are typical application fiel...

  • Page 12

    Standard 25 ics 1-4 b 40-029 e 01/98 1.3 microscope configurations and modules fig. 1-2 standard 25 ics microscope configurations with polarization equipment.

  • Page 13

    Standard 25 ics b 40-029 e 01/98 1-5 cat. No. Configurations standard 25 ics binocular microscope for transmitted-light brightfield and phase contrast using cp achromat objectives 10x, 40x ph2 and 100x 490841 9804 490841 9904 "standard 25 ics " binocular microscope for transmitted-light brightfield ...

  • Page 14

    Standard 25 ics 1-6 b 40-029 e 01/98 1.4 objectives the objectives are the optical centerpiece of the microscope. The following is an example of how objectives can be labelled: cp achromat 10x/0.25 /- where 10x = objective magnification, with a defined color ring on the objective being allocated to ...

  • Page 15

    Standard 25 ics b 40-029 e 01/98 1-7 the following objectives are available for the standard 25 ics microscope: microscopy technique objective magnification/num. Aperture free working distance in mm cover slip thickness d in mm cat.No. Cp-achromat transm.-light brightfield cp-achromat 5x/0.12 11.2 -...

  • Page 16

    Standard 25 ics 1-8 b 40-029 e 01/98 1.5 eyepieces the following eyepieces are offered for the standard 25 ics: eyepiece image angle cat. No. Eyepiece pl 10x/18 br. 39° 444131 9901 eyepiece pl 10x/18 br. Foc. 39° 444132 9901 eyepiece e-pl 10x/20 br. 43° 444231 9901 eyepiece e-pl 10x/20 br. Foc. 43° ...

  • Page 17

    Standard 25 ics b 40-029 e 01/98 1-9 crossline disk / d = 26 mm 474064 crossline micrometer disk 10:100 / d =26 mm gradation length=10 mm increments = 0.1mm gradation tolerance 0.001 mm 474066 9901 net micrometer 12.5x12.5/5;10 / d = 26 mm area 12.5x12.5 mm, divided in fields of 5x5 or 10x10. 474068...

  • Page 18

    Standard 25 ics 1-10 b 40-029 e 01/98 1.7 technical data (1) dimensions (width x depth x height) stand with binocular tube.......................................................................... Approx. 200 x 350 x 450 mm stand with phototube ..........................................................

  • Page 19

    Standard 25 ics b 40-029 e 01/98 1-11 (5) fuses according iec 127: for 230 v........................................................................................................T 0.2 a; 250 v; 5 x 20 mm for 115 v........................................................................................

  • Page 20

    Standard 25 ics 1-12 b 40-029 e 01/98 binocular phototube 35°/20 maximum field number ........................................................................................................... 20 interpupillary distance ........................................................... Can be set between ...

  • Page 21: Start-Up

    Standard 25 ics b 40-029 e 01/98 2-1 start-up contents 2 start-up.......................................................................................................................2-3 2.1 unpacking the instrument.......................................................................................

  • Page 22

    Standard 25 ics 2-2 b 40-029 e 01/98.

  • Page 23

    Standard 25 ics b 40-029 e 01/98 2-3 2 start-up the standard 25 ics microscope, including accessories, is delivered in standard packaging. We would recommend you to keep the packaging material so that the instrument can be stored for a longer period of time or returned to the manufacturer. 2.1 unpac...

  • Page 24

    Standard 25 ics 2-4 b 40-029 e 01/98 2.4 insertion of eyepieces remove both protection caps (2-3/ 1 ) from the binocular tube (2-3/ 2 ). Insert the fixed eyepiece, e.G. Pl 10x/18 br. (2- 3/ 3 ) in the right tube and the focusing eyepiece pl 10x/18 br. Foc. (2-3/ 4 ) in the left tube. The focusing ey...

  • Page 25

    Standard 25 ics b 40-029 e 01/98 2-5 2.4.2 compensation of ametropia when eyepiece reticles are used the correct use of an eyepiece reticle requires two focusing eyepieces, e.G. Pl 10x/18 br. Foc., to enable compensation of ametropia. Use the eyelens of the focusing eyepiece to focus on the line fig...

  • Page 26

    Standard 25 ics 2-6 b 40-029 e 01/98 2.6 attachment of specimen holder to mechanical stage 75x30 r the standard 25 ics features the mechanical stage 75x30 r with ceramic coating (2-6/ 6 ). The specimen is precisely moved in x and y using the user-friendly coaxial drive (2-6/ 4 ). The x/y gradation o...

  • Page 27

    Standard 25 ics b 40-029 e 01/98 2-7 2.7 attachment of condensers 2.7.1 attach condenser 0.9 z unscrew both centering screws (2-7/ 3 ) on the condenser carrier (2-7/ 2 ) until the condenser 0.9 z (2-7/ 9 ) can be easily inserted in the condenser carrier. Insert condenser 0.9 z (2-7/ 9 ) in the conde...

  • Page 28

    Standard 25 ics 2-8 b 40-029 e 01/98 2.8 connecting the instrument to the line check whether the voltage indicated at the rear of the instrument complies with the line voltage! Do not connect the instrument to the line if the instrument voltage and the line voltage are not identical and make sure to...

  • Page 29: Operation

    Standard 25 ics b 40-029 e 01/98 3-1 operation contents 3 operation ...................................................................................................................3-3 3.1 switch on the instrument........................................................................................

  • Page 30

    Standard 25 ics 3-2 b 40-029 e 01/98.

  • Page 31

    Standard 25 ics b 40-029 e 01/98 3-3 3 operation 3.1 switch on the instrument switch on the instrument via the on/off switch (3-1/ 3 ) on the instrument rear. The green led (3-1/ 2 ) must light up. Set the required brightness via the "brightness" control (3-1/ 1 ). Depending on the application, plac...

  • Page 32

    Standard 25 ics 3-4 b 40-029 e 01/98 3.2 setting of transmitted-light brightfield for kÖhler illumination as described in chapter 2, the microscope is ready for operation and switched on according to section 3.1. If the ph 2 phase stop carrier (3-3/ 4 ) is available on condenser 0.9 z (3-3/ 6 ), it ...

  • Page 33

    Standard 25 ics b 40-029 e 01/98 3-5 the kÖhler illumination principle requires the following settings of the condenser, the luminous-field diaphragm and the aperture diaphragm: move condenser 0.9 z (3-3/ 6 ) to the upper stop position via the condenser drive, swing condenser front lens 0.9 into the...

  • Page 34

    Standard 25 ics 3-6 b 40-029 e 01/98 3.3 setting of transmitted-light phase contrast the phase contrast technique is mainly used to increase the image contrast of unstained specimens. As described in chapter 2, the microscope is ready for operation and switched on according to section 3.1. The micro...

  • Page 35

    Standard 25 ics b 40-029 e 01/98 3-7 complete phase contrast is available only if the dark phase ring in the objective covers the bright phase stop in the condenser completely and precisely (also see fig. 3-7/ b ). To achieve this, the diopter or the centering telescope (3-6/ 3 ) must be inserted in...

  • Page 36

    Standard 25 ics 3-8 b 40-029 e 01/98 3.4 setting of transmitted-light darkfield darkfield is mainly used for small or minute objects such as treponema, spirochaeta, cilia and bacteria, but also for emulsions or unstained objects in watery solutions. The special benefit of the darkfield technique is ...

  • Page 37

    Standard 25 ics b 40-029 e 01/98 3-9 3.5 setting of transmitted-light polarization contrast as described in chapter 2, the microscope is ready for operation and switched on according to section 3.1. Transmitted-light polarization contrast requires the following equipment: - polarizer, oriented in ea...

  • Page 38

    Standard 25 ics 3-10 b 40-029 e 01/98 3.6 measurement of lengths the measurement of lengths using the standard 25 ics requires the following, for example: stage micrometer, positive 5 + 100/100 y d = 0.17 mm and eyepiece crossline micrometer 10:100, d = 26 mm an overview of available stage micromete...

  • Page 39

    Standard 25 ics b 40-029 e 01/98 3-11 3.7 attachment of photomicrography equipment the standard 25 ics microscope with camera tube can be changed from observation to photography via a pushrod (3-11/ 7 or 3-12/ 8 ) (pushrod pulled out for photomicrography). Special adapters allow commercially availab...

  • Page 40

    Standard 25 ics 3-12 b 40-029 e 01/98 various t2 adapters for slr cameras are listed below: t2 adapters to slr cameras cat. No. T2 adapter for contax (contax bayonet) 416010 t2 adapter for olympus om (om bayonet) 416002 t2 adapter for minolta (sr bayonet) 416003 t2 adapter for canon (fd bayonet) 416...

  • Page 41

    Standard 25 ics b 40-029 e 01/98 3-13 3.7.2 attachment of mc 80 dx microscope camera (35 mm film cassette) insert adapter 60 for microscope camera (3- 12/ 5 ) (456006) in camera tube (3-12/ 6 ) and fix it using three hexagonal screws (3-12/ 7 ). Insert projection lens p 2.5x (3-12/ 4 ) in adapter 60...

  • Page 42

    Standard 25 ics 3-14 b 40-029 e 01/98 3.8 attachment of adapters for video cameras the following video adapters and video zoom adapters with 60 mm interface permit the attachment of one-chip b/w and color ccd cameras and three-chip color ccd cameras to the camera tube of the standard 25 ics . Tube a...

  • Page 43

    Standard 25 ics b 40-029 e 01/98 3-15 the connecting piece 60 - 44 also allows video adapters with 44 mm interface to be used with the camera tube of the standard 25 ics with 60 mm interface. Video adapters (cat.No.) suitable for:: comments 456140 connecting piece 60 - 44 microscopes with 60 mm inte...

  • Page 44

    Standard 25 ics 3-16 b 40-029 e 01/98 3.9 insertion of 8x drawing eyepiece the 8x drawing eyepiece (444126) is an accessory for microscopic drawing and can only be used in combination with the binocular phototube 35°/20 1 cs on the standard 25 ics. The eyepiece contains a beam splitter which allows ...

  • Page 45

    Standard 25 ics b 40-029 e 01/98 4-1 care, maintenance and troubleshooting contents 4 care, maintenance and troubleshooting ........................................................4-3 4.1 care and maintenance of the instrument ............................................................................

  • Page 46

    Standard 25 ics 4-2 b 40-029 e 01/98.

  • Page 47

    Standard 25 ics b 40-029 e 01/98 4-3 4 care, maintenance and troubleshooting 4.1 care and maintenance of the instrument maintenance of the standard 25 ics is limited to the following operations: cover the instrument with the dust cover after every use. Do not set up the instrument in a damp room, i....

  • Page 48

    Standard 25 ics 4-4 b 40-029 e 01/98 4.2 troubleshooting troubleshooting is described in further detail using the following two examples: changing the fuses, and changing the 6 v / 20 w halogen lamp. Further measures are summarized under 4.2.3. 4.2.1 changing the fuses switch off the instrument via ...

  • Page 49

    Standard 25 ics b 40-029 e 01/98 4-5 4.2.2 changing the 6 v 20 w halogen lamp the following procedure is required to exchange the lamp: switch off lamp supply via on/off switch (4-1/ 2 ) and allow lamp to cool down for approx. 15 minutes. Disconnect the earth-contact plug (4-1/ 4 ) from the line and...

  • Page 50

    Standard 25 ics 4-6 b 40-029 e 01/98 4.2.3 troubleshooting table problem cause remedy vignetting or unhomogeneous image brightness in the field of view the field of view is not entirely visible the pushrod on the camera tube is not in the correct position (observation/photography) move the pushrod i...

  • Page 51

    Standard 25 ics b 40-029 e 01/98 4-7 problem cause remedy low resolving power and poor image contrast aperture diaphragm opening not set correctly. Set aperture diaphragm opening in accordance with the 2/3 rule or depending on the specimen features, see page 3-5. Condenser not focused correctly and ...

  • Page 52

    Standard 25 ics 4-8 b 40-029 e 01/98 problem cause remedy left and right fields of view cannot be combined into an image interpupillary distance of the binocular tube is not set correctly. Set interpupillary distance correctly, see pages 2-5, 3-4 focusing eyepieces are not set correctly. Set focusin...

  • Page 53

    Standard 25 ics b 40-029 e 01/98 4-9 problem cause remedy the 6 v 20 w halogen lamp flickers, unstable brightness end of average life of 6 v 20 w halogen lamp replace 6 v 20 w halogen lamp, see page 4-5 incorrectly installed or broken line cable correctly connect line cable or replace it, see page 4...

  • Page 54

    Standard 25 ics 4-10 b 40-029 e 01/98 4.3 table of spares, consumables and tools description cat. No. Application 6 v 20 w long-life halogen lamp 380079 9690.000 for the integrated standard 25 ics illumination sw 3 ball-headed screwdriver 000000 0069.551 to change the tubes eyepiece eyecup 444801 00...

  • Page 55

    Standard 25 ics b 40-029 e 01/98 4-11 4.4 requesting service all repairs of mechanical, optical or electronic components inside the instrument and of the electrical components of the standard 25 ics may only be performed by carl zeiss service staff or specially authorized personnel. To ensure the op...

  • Page 56

    Standard 25 ics 4-12 b 40-029 e 01/98.

  • Page 57: Annex

    Standard 25 ics b 40-029 e 01/98 a-1 annex list of abbreviations ..................................................................................................................... A-3 physical and technical units........................................................................................

  • Page 58

    Standard 25 ics a-2 b 40-029 e 01/98.

  • Page 59

    Standard 25 ics b 40-029 e 01/98 a-3 list of abbreviations ac alternating current as aspheric a-plan achromatic objectives featuring improved image flatness (ics line) br. Suitable for eyeglass wearers cb correction blue (conversion filter) ccd charge coupled device cp-achromat achromatic objective ...

  • Page 60

    Standard 25 ics a-4 b 40-029 e 01/98 pcb polychlorinated biphenyl ph phase contrast pl flatfield r right (control on the right of the mechanical stage) slr single lens reflex sk protection class sw wrench opening s/w black-and-white t slow-blow (fuse type) tv television t2-adapter standard adapter f...

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