Abbott CELL-DYN 3000 Operator's Manual - Hemoglobin Measurement
CELL-DYN
3000 System Operator’s Manual
3-25
9140240E — May 1995
Chapter 3 Principles of Operation
Hemoglobin Measurement
Overview
The HGB channel is used for the colorimetric determination of
Hemoglobin.
A 1:251 dilution of the sample is made with the diluent and the
Hemoglobin Lyse reagent in the HGB mixing chamber. The HGB
concentration is measured using a modified hemiglobincyanide method.
A filtered LED with a wavelength of 540 nm is the light source. A
photodetector measures the light that is transmitted.
Hemoglobin Measurement Process
The Hemoglobin Lyse reagent lyses the diluted red blood cells and
converts the Hemoglobin that is released to a cyanide-containing
pigment. The sample is transferred to the Hemoglobin flow cell where
the Hemoglobin concentration is measured. The sample enters the flow
cell from the bottom. This allows any bubbles present to float to the
surface so they will not interfere with the reading.
The LED shines through the flow cell and a 540 nm narrow bandwidth
filter onto a photodetector. The Hemoglobin concentration is directly
proportional to the absorbance of the sample at 540 nm. Five separate
HGB readings are made on each sample and averaged to give the final
HGB sample reading. After the Hemoglobin readings have been made,
the HGB flow cell is rinsed with Hemoglobin Lyse.
The rinse is drained and more HGB Lyse reagent is delivered to the HGB
flow cell. A zero or blank reading is then obtained on the reagent to
provide a reference to which the sample signal is compared. Five
separate blank readings are made on each sample and averaged to give
the final HGB reference reading.
The reference and sample readings are compared to determine the HGB
concentration of the sample. The HGB result is expressed in grams of
Hemoglobin per deciliter of whole blood.
HGB Parameter
The Hemoglobin is directly measured and is expressed in grams of
Hemoglobin per deciliter of whole blood.
HGB Flagging
Operational Messages and Data Flagging
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