Wealtec SpectroArt 200 Installation And Operation Manual - D-3. Kinetics
SpectroArt 200 V1.0
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menu until your preferred assay shows. If you want to measure at other wavelength, touch the
“method”-button until “Custom” shows. Touch the button and key in your preferred wavelength
using the number pad. Next, choose the number of points in your standard curve by touching the
“STD Curve”-button and entering a number (2-10), using the number pad. In the same way,
choose number of blank repetitions (1-10), Standard curve point repetitions (1-10) and the
number of times you want to run your unknown samples (1-10). Exit the protein setup menu and
run your blank sample which is the media you have diluted your samples in. After blanking the
spectrophotometer, touch the “STD”-button and enter the unit. Insert your zero-sample and touch
“OK”. When you have measured the absorbance of all of your samples in the standard curve, a
graph with linear equation and R
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-value will show on the display. To measure your unknown
samples, touch the “Unknown”-button and enter the dilution factor. When you have measured
the sample, the ABS and calculated concentration will show on the display.
D-3. Kinetics
With the kinetics function you can follow an enzymatic reaction over time, for example the
build-up of a product from a substrate. You specify the total time to measure and then at what
intervals to measure within this period. Enter the setup menu to specify at what wavelength to
measure, e.g. the absorbance of the build-up product. You also specify the total time you want to
measure and the interval time, that is, how often the spectrophotometer reads the sample.
D-4. OD600
The OD600 function as applicable for monitoring. e.g. bacterial growth. Enter the OD600
setup-menu and insert the correct conversion factor for the bacteria to be measured, using the
number pad. Run a blank sample and then touch the “Sample”-button. Insert the dilution factor
using the number pad and then measure the OD600-value of your bacteria. SpectroArt 200
calculates the correct concentration of cells per ml by multiplying the OD600 value by the
conversion factor and the dilution factor.
D-5. Wavelength
When you want to collect absorbance data quick and easy you can choose the wavelength
function. This function allows you to measure the absorbance of up to 9 different wavelengths