Summary of AxioObserver D1
Page 2: Start Up Procedure
Zeiss axioobserver.D1 microscope room: 3164a start up procedure 1. Remove dust cover from microscope 2. Toggle fluoarc lamp switch on 3. Toggle power bar switch on 4. Turn microscope on ‐ by pressing power button located on left side of microscope 5. Turn on computer 6. Log on to: observer user acco...
Page 3: Microscope Quick Guide
Microscope quick guide zeiss axioobserver.D1 microscope guide room: 3164a start up procedure 1. Remove dust cover from microscope 2 toggle fluoarc lamp switch on 3 toggle power bar switch on 4 turn microscope on ‐ by pressing power button located on left side of microscope 5 turn on computer 6 log o...
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4. Repeat exposure time measurement for each channel, however, adjust the fine focus for only one channel ie the most important one 5. To capture your final image, press the button located at the bottom of the c tab. Post‐acquisition adjustments brightness/contrast 1. Temporarily turn off the pseudo...
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Microscope controls display screen transmitted light controls reflected light controls light path located on left side of microscope objective type objective na objective contrast method objective magnification condenser contrast method hal lamp voltage tl shutter (open) rl shutter (closed) reflecto...
Page 6: Objectives
Objectives zeiss axioobserver.D1 microscope objectives room: 3164a 1 2 3 a‐plan 10x ld plan‐neofluar 20x plan‐apochromat 20x 0.25 ph1 0.4 korr ph2 ‐ m27 0.8 m27 magnification 10x 20x 20x numerical aperture 0.25 0.4 0.8 working distance (mm) 4.2 7.9 at coverglass 0.75 0.55 coverglass (mm) 0.17 0‐1.5 ...
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Plan‐neofluar 100x 1.3 oil ph3 100x 1.3 0.2 0.17 oil ics **** **** **** *** ‐ ‐ ph3 000000‐1031‐171.
Page 8: Filter Sets
Filter sets zeiss axio observer d1 microscope filters room 3164a 1. Transmitted light 2. Filter set 34 (dapi/bfp) excitation: bp 390/22 beam splitter: ft 420 emission: bp 460/50 3. Filter set 38 (cy2/gfp) excitation: bp 470/40 beam splitter: ft 495 emission: bp 525/50 4. Filter set 46 he (yfp) excit...
Page 9: Filter Set
Filter set.
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Https://www.Micro‐shop.Zeiss.Com/?P=us&f=f.
Page 12: Attention Users!!
Attention users!! Fluoarc lamp: the fluoarc lamp must be on for at least 30 minutes before being turned off and must not be turned on again within 1 hour of being turned off. 1. When starting, before you turn on the fluoarc lamp, make sure it has been off for at least 1 hour from previous use. 2. Wh...
Page 13: Computer:
Computer: this computer is to be used only for image acquisition. No other work is permitted!! Immersion oil rules: always keep immersion oil bottle closed. This prevents water evaporation and maintains the integrity of the oil keep oil bottle clean. Oil should never be found along the sides of ...