Zeiss AxioObserver D1 User Manual - Computer:
Computer:
This computer is to be used only for image acquisition. No other work is
permitted!!
Immersion Oil Rules:
Always keep immersion oil bottle closed. This prevents water
evaporation and maintains the integrity of the oil
Keep oil bottle clean. Oil should never be found along the sides of
the bottle or around the neck
Apply only a small amount of oil to your coverslip
Always load your sample on the microscope stage with the
objective lowered to the lowest focus position
Oil should never reach the outer rim of the objectives. The felt
protectors should never become saturated with oil
Always remove oil using dry Lens Paper from the objective when
changing slides. Re‐apply a small amount of new oil to the new slide
If you are imaging a sample using an oil objective, do not toggle
between objectives. Image with that objective only!
When done, thoroughly wipe off oil objective using Lens Paper
Do not get oil on any air objectives!
Coverslip
Oil droplet