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Zeiss Axiovert 200 Operating Manual

Other manuals for Axiovert 200: Quick Start Manual
Manual is about: Inverted Microscopes

Summary of Axiovert 200

  • Page 1

    Operating manual axiovert 200 / axiovert 200 m inverted microscopes.

  • Page 2

    Introduction carl zeiss copyright axiovert 200 0-2 b 40-080 e 03/01 knowledge of this manual is required for the operation of the instrument. Would you please therefore make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the ins...

  • Page 3

    Introduction axiovert 200 contents carl zeiss b 40-080 e 03/01 0-3 contents page introduction ........................................................................................................ 0-2 copyright ..........................................................................................

  • Page 4

    Introduction carl zeiss contents axiovert 200 0-4 b 40-080 e 03/01 page chapter 2 start-up.................................................................................................................. 2-5 2.1 unpacking and installation of the microscope..............................................

  • Page 5

    Introduction axiovert 200 contents carl zeiss b 40-080 e 03/01 0-5 page 2.13 illuminator n hbo 103 .............................................................................................. 2-23 2.13.1 attachment / change of the hbo 103 w/2 mercury pressure short-arc lamp............... 2-23 2.1...

  • Page 6

    Introduction carl zeiss contents axiovert 200 0-6 b 40-080 e 03/01 page chapter 4 care, maintenance, troubleshooting and service.................................... 4-3 4.1 care .............................................................................................................................

  • Page 7

    Introduction axiovert 200 notes on instrument safety carl zeiss b 40-080 e 03/01 0-7 notes on instrument safety the axiovert 200 / axiovert 200 m microscopes were designed, produced and tested in compliance with din 61010-1 (iec 1010-1), safety requirements for electrical measuring, control and labo...

  • Page 8

    Introduction carl zeiss notes on instrument safety axiovert 200 0-8 b 40-080 e 03/01 the axiovert 200 microscopes, including original accessories, may only be used for the microscope techniques described in this manual. Particular attention must be paid to the following notes: the manufacturer canno...

  • Page 9

    Introduction axiovert 200 notes on instrument safety carl zeiss b 40-080 e 03/01 0-9 gas discharge lamps, e.G. Hbo 100, emit ultraviolet radiation which can cause burns on the eyes and skin. Therefore, never look directly into the light of these lamps and avoid direct, unprotected incidence of their...

  • Page 10

    Introduction carl zeiss notes on warranty axiovert 200 0-10 b 40-080 e 03/01 notes on warranty the manufacturer guarantees that the instrument has no material and production defects when delivered. You must inform us of any defects immediately and we must do anything to minimize the damage. If the m...

  • Page 11

    Introduction axiovert 200 overall view of the axiovert 200 and axiovert 200 m carl zeiss b 40-080 e 03/01 0-11.

  • Page 12

    Introduction carl zeiss microscopy in transmitted-light brightfield in a few steps axiovert 200 0-12 b 40-080 e 03/01 microscopy in transmitted-light brightfield in a few steps ☞ before starting to use the axiovert 200, make sure to read the notes on instrument safety and the chapters entitled " ins...

  • Page 13

    Introduction axiovert 200 microscopy in transmitted-light brightfield in a few steps carl zeiss b 40-080 e 03/01 0-13 1 on / off switch 2 objective nosepiece 3 compartment for slider analyzer 4 setting wheel for optovar turret 5 reflector turret 6 focusing drive coarse / fine 7 hal on / off switch 8...

  • Page 14

    Introduction carl zeiss microscopy in transmitted-light brightfield in a few steps axiovert 200 0-14 b 40-080 e 03/01.

  • Page 15: Instrument Description

    Instrument description axiovert 200 contents / list of illustrations carl zeiss b 40-080 e 03/01 1-1 instrument description contents 1 instrument description .......................................................................................... 1-3 1.1 name and intended application.................

  • Page 16

    Instrument description carl zeiss contents / list of illustrations axiovert 200 1-2 b 40-080 e 03/01.

  • Page 17

    Instrument description axiovert 200 name and intended application carl zeiss b 40-080 e 03/01 1-3 1 instrument description 1.1 name and intended application manufacturer's name: inverted microscope for transmitted light and epifluorescence brief name: axiovert 200 (manual version) axiovert 200 m (mo...

  • Page 18

    Instrument description carl zeiss name and intended application axiovert 200 1-4 b 40-080 e 03/01 typical fields of application: observation of intracellular processes in living cell cultures, cell/cell interactions, motility, growth, measurements of potential, drug detection, microinjection, ivf (i...

  • Page 19

    Instrument description axiovert 200 instrument description and main features carl zeiss b 40-080 e 03/01 1-5 1.2 instrument description and main features the axiovert 200 / axiovert 200 m is available either as manual or as motorized version. The accessory components are part of a modular system. Fo...

  • Page 20

    Instrument description carl zeiss microscope configurations and modules axiovert 200 1-6 b 40-080 e 03/01 1.3 microscope configurations and modules fig. 1-1 microscope configurations and modules (sheet 1).

  • Page 21

    Instrument description axiovert 200 microscope configurations and modules carl zeiss b 40-080 e 03/01 1-7 fig. 1-2 microscope configurations and modules (sheet 2).

  • Page 22

    Instrument description carl zeiss microscope configurations and modules axiovert 200 1-8 b 40-080 e 03/01 fig. 1-3 microscope configurations and modules (sheet 3).

  • Page 23

    Instrument description axiovert 200 microscope configurations and modules carl zeiss b 40-080 e 03/01 1-9 fig. 1-4 microscope configurations and modules (sheet 4).

  • Page 24

    Instrument description carl zeiss objectives axiovert 200 1-10 b 40-080 e 03/01 1.4 objectives the objectives are the optical centerpiece of the microscope. The following is an example of how objectives can be labelled: a-plan 10 × /0.20 hd ∞ /- key: 10 × objective magnification, with a color ring o...

  • Page 25

    Instrument description axiovert 200 objectives carl zeiss b 40-080 e 03/01 1-11 the following objectives are available for the axiovert 200 / axiovert 200 m microscope: objective type magnification/ numeric aperture cover slip thickness d in mm free working distance a in mm contrasting cat. No. A-pl...

  • Page 26

    Instrument description carl zeiss eyepieces / condensers axiovert 200 1-12 b 40-080 e 03/01 1.5 eyepieces the following eyepieces are available for the axiovert 200 and axiovert 200 m: eyepiece type image angle cat. No. Eyepiece w-pl 10 × /23 br. Foc. 24.7° 455043-0000-000 eyepiece w-pl 10 × /23 br....

  • Page 27

    Instrument description axiovert 200 specimen stages and mounting frames / binocular tubes carl zeiss b 40-080 e 03/01 1-13 1.7 specimen stages and mounting frames the axiovert 200 microscopes can be equipped with the following specimen stages and mounting frames: description cat. No. Specimen stage ...

  • Page 28

    Instrument description carl zeiss technical data axiovert 200 1-14 b 40-080 e 03/01 1.9 technical data dimensions (width x depth x height) stand axiovert 200.................................................................................... Approx. 295 × 805 × 707 mm weight axiovert 200 without flu...

  • Page 29

    Instrument description axiovert 200 technical data carl zeiss b 40-080 e 03/01 1-15 line frequency ...................................................................................................................... 50 to 60 hz power consumption with internal power units axiovert 200 ................

  • Page 30

    Instrument description carl zeiss technical data axiovert 200 1-16 b 40-080 e 03/01.

  • Page 31: Start-Up

    Start-up axiovert 200 contents carl zeiss b 40-080 e 03/01 2-1 start-up contents 2 start-up....................................................................................................................... 2-5 2.1 unpacking and installation of the microscope........................................

  • Page 32

    Start-up carl zeiss contents axiovert 200 2-2 b 40-080 e 03/01 2.8 connection to the line................................................................................................... 2-18 2.9 interfaces of the axiovert 200 m ........................................................................

  • Page 33

    Start-up axiovert 200 list of illustrations carl zeiss b 40-080 e 03/01 2-3 list of illustrations fig. 2-1 installation of microscope ................................................................................................2-5 fig. 2-2 attachment of binocular tube ...............................

  • Page 34

    Start-up carl zeiss list of illustrations axiovert 200 2-4 b 40-080 e 03/01.

  • Page 35

    Start-up axiovert 200 unpacking and installation of microscope carl zeiss b 40-080 e 03/01 2-5 2 start-up on account of the complexity of the equipment and to ensure proper functioning, installation and initial start-up of the axiovert 200 or axiovert 200 m at your site will be performed entirely by...

  • Page 36

    Start-up carl zeiss attachment of binocular (photo)tube axiovert 200 2-6 b 40-080 e 03/01 2.2 attachment of binocular (photo) tube all the binocular tubes listed in the microscope configuration can be attached to the axiovert 200 and axiovert 200 m as described below. Proceed as follows to attach a ...

  • Page 37

    Start-up axiovert 200 attachment of binocular (photo)tube carl zeiss b 40-080 e 03/01 2-7 2.2.2 inserting the eyepiece reticle the eyepieces w-pl 10x/23 br. Foc. Are intended for use with eyepiece reticles. The slight image shift caused by the additional path through the glass is taken into account ...

  • Page 38

    Start-up carl zeiss attachment of transmitted-light illumination axiovert 200 2-8 b 40-080 e 03/01 2.3 attachment of transmitted-light illumination 2.3.1 attachment of carrier for trans- mitted-light illumination (100 w) • attach carrier (2-5/ 1 ) to the relevant contact surface on the rear of the s...

  • Page 39

    Start-up axiovert 200 screw in objectives carl zeiss b 40-080 e 03/01 2-9 2.4 screw in objectives • remove dust caps (2-7/ 1 ) from the respective openings in the nosepiece. • remove objectives (2-7/ 2 ) from the case and screw them in the nosepiece (2-7/ 3 ), starting with position 1 (see engraved ...

  • Page 40

    Start-up carl zeiss attachment of microscope stages axiovert 200 2-10 b 40-080 e 03/01 2.5 attachment of microscope stages 2.5.1 attachment of mechanical stage 130x85 r/l and mounting frame for mechanical stage (k) the mechanical stage is mounted to the stand directly above three contact points with...

  • Page 41

    Start-up axiovert 200 attachment of microscope stages carl zeiss b 40-080 e 03/01 2-11 because of the large travel range of the scanning stage, it may happen that the stage frame collides with the objectives at the end of the stage travel. 2.5.3 attachment of specimen stage 250x230, object guide and...

  • Page 42

    Start-up carl zeiss attachment of microscope stages axiovert 200 2-12 b 40-080 e 03/01 2.5.4 attachment of heating stage the heating stage is attached to the contact points of the stand using three spacer disks. • remove any available microscope stage and additional mounting components. • place spac...

  • Page 43

    Start-up axiovert 200 attachment of condensers carl zeiss b 40-080 e 03/01 2-13 2.6 attachment of condensers 2.6.1 condensers for the axiovert 200 • insert condenser (2-13/ 1 ) into the condenser carrier on the carrier for transmitted-light illumination with its dovetail pointing upwards. Make sure ...

  • Page 44

    Start-up carl zeiss attachment of condensers axiovert 200 2-14 b 40-080 e 03/01 • insert condenser adapter (2-14/ 1 ) into the condenser carrier on the carrier for transmitted-light illumination with its dovetail pointing upwards. Make sure that the orientation pin of the condenser is positioned at ...

  • Page 45

    Start-up axiovert 200 reflector turret carl zeiss b 40-080 e 03/01 2-15 2.7 reflector turret 2.7.1 attachment of reflector turret the manual reflector turret can be pushed into the stand either from the right or from the left, depending on which side is accessible. The motorized reflector turret can...

  • Page 46

    Start-up carl zeiss reflector turret axiovert 200 2-16 b 40-080 e 03/01 • to remove a reflector module no longer required, first pull it out of the upper spring clamps and then from the lower ones. • when equipment has been finished, attach the plastic hood again and press it downwards until the hol...

  • Page 47

    Start-up axiovert 200 reflector turret carl zeiss b 40-080 e 03/01 2-17 • hold both halves of the reflector module together, turn them around into the installation position and put them down. • now tilt the upper module half (2-20/ 1 ) upwards and lift it out of the holding elements (2-20/ 5b ) of t...

  • Page 48

    Start-up carl zeiss connection to the line axiovert 200 2-18 b 40-080 e 03/01 2.8 connection to the line • connect microscope socket (2-21/ 1 ) to the line socket via a line cable. The microscope can be connected to line voltage between 90 and 264 vac, 50 - 60 hz. The microscope is equipped with a w...

  • Page 49

    Start-up axiovert 200 interfaces ... / microscope and power supply ... Carl zeiss b 40-080 e 03/01 2-19 2.9 interfaces of the axiovert 200 m prior to connecting any components, switch off the microscope. The axiovert 200 m is connected to a pc via the rs 232 interface (2-23/ 2 ). Motorized component...

  • Page 50

    Start-up carl zeiss hal 100 w halogen illuminator axiovert 200 2-20 b 40-080 e 03/01 2.12 hal 100 halogen illuminator 2.12.1 change / attachment of the hal 100 halogen lamp • switch off the microscope, remove connector (2-27/ 4 ) from the 12 v / 100 w socket (2-27/ 5 ) and allow the illuminator to c...

  • Page 51

    Start-up axiovert 200 hal 100 w halogen illuminator carl zeiss b 40-080 e 03/01 2-21 2.12.2 coarse alignment of halogen illuminator • loosen the clamping screw (2-27/ 2 ) and remove the halogen illuminator from the carrier for transmitted-light illumination. • switch on the microscope. • direct ligh...

  • Page 52

    Start-up carl zeiss hal 100 w halogen illuminator axiovert 200 2-22 b 40-080 e 03/01 2.12.4 fine alignment of halogen illuminator fine alignment requires the diffusion disk to be removed: • loosen clamping screw of hal 100 w (2-28/ 1 ) and remove the illuminator from the carrier for transmitted-ligh...

  • Page 53

    Start-up axiovert 200 illuminator n hbo 103 carl zeiss b 40-080 e 03/01 2-23 2.13 illuminator n hbo 103 2.13.1 attachment / change of the hbo 103 w/2 mercury pressure short-arc lamp the illuminator n hbo 103 and the hbo 103 w/2 mercury pressure short-arc lamp are supplied to the customer in separate...

  • Page 54

    Start-up carl zeiss illuminator n hbo 103 axiovert 200 2-24 b 40-080 e 03/01 • use focusing knob (2-30/ 3 ) to bring collector to the position at the very front (in the direction of the light). • use ball-headed screwdriver to loosen clamping screw for lamp housing (2-30/ 2 ) and carefully remove la...

  • Page 55

    Start-up axiovert 200 illuminator n hbo 103 carl zeiss b 40-080 e 03/01 2-25 2.13.2 coarse alignment of illuminator n hbo 103 the following settings for adjustment of the n hbo 103 illuminator are performed in two steps: the coarse adjustment and the subsequent fine adjustment using the adjustment a...

  • Page 56

    Start-up carl zeiss illuminator n hbo 103 axiovert 200 2-26 b 40-080 e 03/01 ☞ if required, the above settings for coarse adjustment of the n hbo 103 illuminator can be performed repeatedly. During coarse adjustment of the focal spots, adjusting screws (2-32/ 1 and 2 ) marked red must not be changed...

  • Page 57

    Start-up axiovert 200 illuminator n hbo 103 carl zeiss b 40-080 e 03/01 2-27 2.13.4 fine adjustment of illuminator n hbo 103 using the adjusting aid attach illuminator n hbo 103 to the microscope stand, tighten the clamping screw, and switch on the illuminator. • pull out adjusting aid (2-37/ 7 ) fr...

  • Page 58

    Start-up carl zeiss attachment of aqua stop axiovert 200 2-28 b 40-080 e 03/01 2.14 attachment of aqua stop aqua stop can be used to protect the objective and the nosepiece from liquids. • remove the microscope stage. • put collecting trough 2-38/ 5 ) onto nosepiece carrier (2-38/ 2 ) and screw it o...

  • Page 59

    Start-up axiovert 200 attachment of aqua stop carl zeiss b 40-080 e 03/01 2-29 • you can fix the drain hose using the provided clips. ☞ fix the hose in such a way, that the drain gutter is not bent in focusing. • attach the microscope stage again. After any accident with liquids, you are strongly ad...

  • Page 60

    Start-up carl zeiss attachment of aqua stop axiovert 200 2-30 b 40-080 e 03/01 objective type magnification / num. Aperture contrasting hole size silicone ring size cat. No. Plan-neofluar plan-neofluar 5x/0.15 2 1 440320-0000-000 plan-neofluar 5x/0.15 ph1 2 1 440321-0000-000 plan-neofluar 10x/0.30 2...

  • Page 61: Operation

    Operation axiovert 200 contents carl zeiss b 40-080 e 03/01 3-1 operation contents 3 operation ................................................................................................................... 3-3 3.1 axiovert 200 (manual) ..............................................................

  • Page 62

    Operation carl zeiss list of illustrations axiovert 200 3-2 b 40-080 e 03/01 list of illustrations fig. 3-1 operation and function controls on the axiovert 200 (manual) ......................................... 3-4 fig. 3-2 nosepiece with mounts for dic slider ..........................................

  • Page 63

    Operation axiovert 200 carl zeiss b 40-080 e 03/01 3-3 3 operation the axiovert 200 microscopes are offered in a manual version and in a version with motorized functions and components. In the "operation" chapter, the manual setting and operation functions are explained first, and the motorized (cod...

  • Page 64

    Operation carl zeiss axiovert 200 axiovert 200 3-4 b 40-080 e 03/01 3.1 axiovert 200 (manual) 3.1.1 operation and function controls on the axiovert 200 (manual) fig. 3-1 operation and function controls on the axiovert 200 (manual).

  • Page 65

    Operation axiovert 200 axiovert 200 carl zeiss b 40-080 e 03/01 3-5 key to fig. 3-1: 1 on / off switch 2 nosepiece 3 compartment for analyzer slider (can be equipped from the right or left) 4 drive controls for xy-movement of the mechanical stage 5 sideport right 6 setting wheel for optovar turret (...

  • Page 66

    Operation carl zeiss axiovert 200 axiovert 200 3-6 b 40-080 e 03/01 on / off switch (3-1/ 1 ) − position 0 = instrument switched off − position i = instrument switched on, switch lights up key hal on/off (3-1/ 10 ) − brief pressing switches the halogen illuminator on or off alternately. − long press...

  • Page 67

    Operation axiovert 200 axiovert 200 carl zeiss b 40-080 e 03/01 3-7 nosepiece with objectives (3-1/ 2 ) − 6-position nosepiece h, dic (3-2/ 1 ) with compartments for dic slider (3-2/ 2 ) in all objective positions. − fast change of objectives through rotation of the nosepiece via the setting ring (3...

  • Page 68

    Operation carl zeiss axiovert 200 axiovert 200 3-8 b 40-080 e 03/01 binocular tubes (3-1/ 13 ) − the binocular tubes offered permit the individual setting of the interpupillary distance and the viewing height within defined limits. Binocular tube 45°/23 with manual shutter vis (3-3) − shutter switch...

  • Page 69

    Operation axiovert 200 axiovert 200 carl zeiss b 40-080 e 03/01 3-9 binocular component of the tubes (3-1/ 14 ) − the eyepiece distance is matched to the individual interpupillary distance by swinging the eyepiece tubes symmetrically towards one another (3-5/ a and 3-5/ b ). − two vertical adjustmen...

  • Page 70

    Operation carl zeiss axiovert 200 axiovert 200 3-10 b 40-080 e 03/01 condensers (3-1/ 23 ) depending on their type, condensers (3-7/ 1 ) are equipped as follows: − 5- or 6-position turret for: brightfield: h phase contrast: ph0, ph1, ph2, ph3 with centerable stops interference contrast: dic varel co...

  • Page 71

    Operation axiovert 200 axiovert 200 carl zeiss b 40-080 e 03/01 3-11 centering screw for condenser (3-1/ 18 and 22 ) − screws for condenser centering attached to both sides of the carrier for transmitted-light illumination / condenser. Lever for luminous-field diaphragm, transmitted light (3-1/ 20 )...

  • Page 72

    Operation carl zeiss axiovert 200 axiovert 200 3-12 b 40-080 e 03/01 baseport (3-1/ 34 ) − port for the connection of documentation equipment below the axiovert 200. Setting knob for frontport / baseport / vis (3-1/ 32 ) − selects the beam splitting ratio for frontport, baseport and visual observati...

  • Page 73

    Operation axiovert 200 axiovert 200 carl zeiss b 40-080 e 03/01 3-13 5-position reflector turret (3-1/ 7 ) − accepts the maximum of 5 reflector modules for epi-fluorescence. − fast change of reflector position by turning the setting ring of the reflector turret. − the activated reflector position is...

  • Page 74

    Operation carl zeiss axiovert 200 axiovert 200 3-14 b 40-080 e 03/01 3.1.2 switching on and basic settings on the axiovert 200 (manual) • switch on the microscope with the on/off switch on the right side (3-11/1). The indicator light in the switch lights up. The microscope is ready for operation aft...

  • Page 75

    Operation axiovert 200 axiovert 200 carl zeiss b 40-080 e 03/01 3-15 3.1.2.2 light manager the light manager is automatically activated each time the axiovert 200 is switched on. The light manager automatically sets the illumination intensity of the halogen illuminator last saved for each individual...

  • Page 76

    Operation carl zeiss axiovert 200 m axiovert 200 3-16 b 40-080 e 03/01 3.2 axiovert 200 m (motorized) 3.2.1 operation and function controls on the axiovert 200 (motorized) fig. 3-13 operation and function controls on the axiovert 200 m (motorized).

  • Page 77

    Operation axiovert 200 axiovert 200 m carl zeiss b 40-080 e 03/01 3-17 key to fig. 3-13: 1 on / off switch 2 nosepiece (motorized) 3 reflector turret (motorized) 4 objective keys (forward and backward) 5 reflector keys (forward and backward) 6 focusing drive coarse / fine (motor-supported) 7 zero ke...

  • Page 78

    Operation carl zeiss axiovert 200 m axiovert 200 3-18 b 40-080 e 03/01 focus key - work / load (3-13/ 8 ) − load key brings the nosepiece to the lowest position. Load position appears in the lower line of the lcd display. The lower lcd line blinks every second as a warning sign. − work key pressed b...

  • Page 79

    Operation axiovert 200 axiovert 200 m carl zeiss b 40-080 e 03/01 3-19 lcd display (3-13/ 17 ) − two-line display with 16 characters each attached to the carrier for transmitted-light illumination / condenser. − display of objective position (3-6/ 1 ), magnification (3-6/ 2 ) and contrasting techniq...

  • Page 80

    Operation carl zeiss axiovert 200 m axiovert 200 3-20 b 40-080 e 03/01 motorized condensers (3-13/ 16 ) motorized condenser, equipped with: − 6-position turret for: brightfield: h phase contrast: ph1, ph2, ph3 with centerable stops interference contrast: dic i, dic ii − if a motorized condenser is r...

  • Page 81

    Operation axiovert 200 axiovert 200 m carl zeiss b 40-080 e 03/01 3-21 left / right key for the motorized switching of the sideport turret (3-13/ 13 ) − key to switch the beam splitting ratio for sideport (doc) right, sideport (doc) left and vis (visual observation through the binocular). − five mot...

  • Page 82

    Operation carl zeiss axiovert 200 m axiovert 200 3-22 b 40-080 e 03/01 motorized 5-position reflector turret (3-13 /3 ) − accepts the maximum of 5 reflector modules for epi-fluorescence. − fast change of reflector position through pressing the key (forward or backward) on the right of the stand (3-1...

  • Page 83

    Operation axiovert 200 axiovert 200 m carl zeiss b 40-080 e 03/01 3-23 3.2.2 switching on and basic settings on the axiovert 200 m (motorized) • switch on the microscope with the on/off switch on the right side. The indicator light in the switch lights up. Initialization of the instrument follows. A...

  • Page 84

    Operation carl zeiss axiovert 200 m axiovert 200 3-24 b 40-080 e 03/01 setting of nosepiece equipment • press one of the two objective keys. Change to nosepiece programming is made, and set objective is displayed in the upper line of the lcd display. The set nosepiece position, the magnification and...

  • Page 85

    Operation axiovert 200 axiovert 200 m carl zeiss b 40-080 e 03/01 3-25 setting of z focus factors and parfocality the matching of focus positions (parfocality) and the adaptation of the focus speed (z-focus factors) to the objective magnification are normally only performed when the microscope is us...

  • Page 86

    Operation carl zeiss axiovert 200 m axiovert 200 3-26 b 40-080 e 03/01 deactivation of set mode and saving of settings the set mode can be exited after conclusion of the settings. The settings / changes performed are automatically stored when the mode is exited. • to exit the set mode and return to ...

  • Page 87

    Operation axiovert 200 axiovert 200 m carl zeiss b 40-080 e 03/01 3-27 3.2.2.3 light manager the light manager is applied with the axiovert 200 m (storage of set values) in the same way as with the manual version. However, the functionality of the light manager is considerably greater in the axiover...

  • Page 88

    Operation carl zeiss illumination and contrasting techniques axiovert 200 3-28 b 40-080 e 03/01 3.3 illumination and contrasting techniques 3.3.1 setting of transmitted-light brightfield for kÖhler illumination 3.3.1.1 general principle transmitted-light brightfield microscopy is the most usual of a...

  • Page 89

    Operation axiovert 200 illumination and contrasting techniques carl zeiss b 40-080 e 03/01 3-29 3.3.1.3 transmitted-light brightfield setting according to kÖhler on the axiovert 200 (manual) − put the axiovert 200 (manual) in operation as described in chapter 2. − switch on the axiovert 200 (manual)...

  • Page 90

    Operation carl zeiss illumination and contrasting techniques axiovert 200 3-30 b 40-080 e 03/01 1 on / off switch 2 nosepiece 3 compartment for slider analyzer 4 setting wheel for optovar turret 5 reflector turret 6 focusing drive coarse / fine 7 hal on/off key 8 toggle switch for illumination inten...

  • Page 91

    Operation axiovert 200 illumination and contrasting techniques carl zeiss b 40-080 e 03/01 3-31 • swing out 3-position filter changer (3-16/ 14 ). • place a high-contrast specimen on the microscope stage (3-16/ 21 ). • match the eyepiece distance (interpupillary distance) to the user's individual in...

  • Page 92

    Operation carl zeiss illumination and contrasting techniques axiovert 200 3-32 b 40-080 e 03/01 3.3.1.4 transmitted-light brightfield configuration of the axiovert 200 m each axiovert 200 m microscope can be configured to permit the transmitted-light brightfield technique. 3.3.1.5 transmitted-light ...

  • Page 93

    Operation axiovert 200 illumination and contrasting techniques carl zeiss b 40-080 e 03/01 3-33 3.3.2 setting of transmitted-light phase contrast 3.3.2.1 general principle the phase contrast technique is ideal for examinations of thin, unstained specimens, e.G. Culture cells. The human eye is unable...

  • Page 94

    Operation carl zeiss illumination and contrasting techniques axiovert 200 3-34 b 40-080 e 03/01 3.3.2.3 setting of transmitted-light phase contrast on the axiovert 200 / axiovert 200 m • swing phase contrast objective, e.G. Ph 1, into the beam path. • switch phase stop with the same description as t...

  • Page 95

    Operation axiovert 200 illumination and contrasting techniques carl zeiss b 40-080 e 03/01 3-35 3.3.3 setting of differential interference contrast (dic) in transmitted light 3.3.3.1 general principle the transmitted-light dic technique permits high-contrast 3d images of transparent specimen details...

  • Page 96

    Operation carl zeiss illumination and contrasting techniques axiovert 200 3-36 b 40-080 e 03/01 (2) transmitted-light dic with analyzer slider ±±±± 30° (de senarmont) when the analyzer slider ± 30° is used, the dic slider must be brought to the center position first. • swing in the polarizer and bri...

  • Page 97

    Operation axiovert 200 illumination and contrasting techniques carl zeiss b 40-080 e 03/01 3-37 1 polarizer d (fixed, optional: rotary) 2 condenser 3 dic slider 4 analyzer slider, fixed 5 analyzer slider ± 30° fig. 3-20 components for the transmitted-light dic technique on the axiovert 200

  • Page 98

    Operation carl zeiss illumination and contrasting techniques axiovert 200 3-38 b 40-080 e 03/01 3.3.4 setting of varel contrast in transmitted light 3.3.4.1 general principle varel contrast provides a relief-like image of objects and can be used as an alternative to phase contrast. Varel contrast ca...

  • Page 99

    Operation axiovert 200 illumination and contrasting techniques carl zeiss b 40-080 e 03/01 3-39 the specimen field with the relevant pupil image of the varel diaphragm is shown in fig. 3-22. In the pupil image, the diaphragm appears rotated by 180°. ☞ − shifting the varel illumination to outside the...

  • Page 100

    Operation carl zeiss illumination and contrasting techniques axiovert 200 3-40 b 40-080 e 03/01 3.3.5 setting of fluorescence contrast in reflected light 3.3.5.1 general principle the epi-fluorescence technique permits high-contrast images of fluorescent substances in typical fluorescence colors. In...

  • Page 101

    Operation axiovert 200 illumination and contrasting techniques carl zeiss b 40-080 e 03/01 3-41 1 n hbo 103 fluorescence illuminator 2 iris stop slider (aperture diaphragm) 3 iris stop slider (luminous-field diaphragm) 4 nosepiece 5 reflector turret 6 fl on / off key fig. 3-24 components for epi-flu...

  • Page 102

    Operation carl zeiss illumination and contrasting techniques axiovert 200 3-42 b 40-080 e 03/01 • switch on the nhbo 103 fluorescence illuminator (3-24/ 1 ) via the power supply and allow it to heat to operating temperature for approx. 15 minutes. • select the fl reflector module in the reflector tu...

  • Page 103

    Operation axiovert 200 documentation carl zeiss b 40-080 e 03/01 3-43 3.4 documentation depending on the instrument configuration, the axiovert 200 (manual) and axiovert 200 m microscopes can be equipped with up to five documentation ports. − frontport for the connection of a slr camera, a video or ...

  • Page 104

    Operation carl zeiss documentation axiovert 200 3-44 b 40-080 e 03/01 order no. / viewed with / intermediate / direction of viewing direction, description switch position image / monitor motion of object schematic 000000-1005-827 eyepieces (100% vis) binocular tube 000000-1005-828 100% vis : 0% doc ...

  • Page 105

    Operation axiovert 200 documentation carl zeiss b 40-080 e 03/01 3-45 order no. / viewed with / intermediate / direction of viewing direction, description switch position image / monitor motion of object schematic 000000-1005-824 100% doc baseport camera adapters working without intermediate imaging...

  • Page 106

    Operation carl zeiss documentation axiovert 200 3-46 b 40-080 e 03/01 • attach the premounted camera system to the frontport, align it horizontally and tighten clamping screw (3-25/ 5 ) using the sw 3 ball-headed screwdriver. • switch on the microscope and, when using a color film (artificial light)...

  • Page 107: Service

    Care, maintenance, troubleshooting and service axiovert 200 contents / list of illustrations carl zeiss b 40-080 e 03/01 4-1 care, maintenance, troubleshooting and service contents 4 care, maintenance, troubleshooting and service......................................... 4-3 4.1 care ...................

  • Page 108

    Care, maintenance, troubleshooting and service carl zeiss contents / list of illustrations axiovert 200 4-2 b 40-080 e 03/01.

  • Page 109

    Care, maintenance, troubleshooting and service axiovert 200 care carl zeiss b 40-080 e 03/01 4-3 4 care, maintenance, troubleshooting and service 4.1 care care of the instruments is limited to the following operations: • cover the instrument with the dust cover after every use. • do not install inst...

  • Page 110

    Care, maintenance, troubleshooting and service carl zeiss maintenance axiovert 200 4-4 b 40-080 e 03/01 4.2 maintenance 4.2.1 performing checks the following checks should be performed regularly every six months. General • check line cables and plugs for defects. • if any damage is visible, switch o...

  • Page 111

    Care, maintenance, troubleshooting and service axiovert 200 maintenance carl zeiss b 40-080 e 03/01 4-5 4.2.3 changing the fuses on the ebq 100 dc power supply the fuse holder for the fuses f1 and f2 is positioned on the rear of the power supply. It is combined with the instrument socket and contain...

  • Page 112

    Care, maintenance, troubleshooting and service carl zeiss service axiovert 200 4-6 b 40-080 e 03/01 4.3 service all repairs of optical or electronic components inside the instrument or any repair of the power supply may only be performed by service staff or specially authorized personnel. If service...

  • Page 113: Annex:

    Annex axiovert 200 overview carl zeiss b 40-080 e 03/01 a-1 annex: list of abbreviations .......................................................................................................................... A-3 list of key words......................................................................

  • Page 114

    Annex carl zeiss overview axiovert 200 a-2 b 40-080 e 03/01.

  • Page 115

    Annex axiovert 200 list of abbreviations carl zeiss b 40-080 e 03/01 a-3 list of abbreviations a free working distance ac alternating current a-plan achromatic objectives featuring improved image flatness (ics line) br. Suitable for eyeglass wearers can bus controller area network communication bus ...

  • Page 116

    Annes carl zeiss list of abbreviations axiovert 200 a-4 b 40-080 e 03/01 ph phase contrast pl flatfield sb sub-bus slr single lens reflex sw wrench opening t slow-blow (fuse type) tv television ul underwriter laboratories vis visual xbo xenon short-arc lamp.

  • Page 117

    Annex axiovert 200 list of key words carl zeiss b 40-080 e 03/01 a-5 list of key words page 3 3.200k .....................................................................................................................................3-6, 3-17 a adjusting aid ...........................................

  • Page 118

    Annex carl zeiss list of key words axiovert 200 a-6 b 40-080 e 03/01 f fluorescence contrast....................................................................................................................... 3-40 fluorescence illuminator .............................................................

  • Page 119

    Annex axiovert 200 list of key words carl zeiss b 40-080 e 03/01 a-7 p parfocality .......................................................................................................................................3-25 performing checks ..............................................................

  • Page 120

    Annex carl zeiss list of key words axiovert 200 a-8 b 40-080 e 03/01.