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  4. Axiovert 200
  5. Quick Start Manual

Zeiss Axiovert 200 Quick Start Manual

Other manuals for Axiovert 200: Operating Manual
Manual is about: Inverted Microscopes

Summary of Axiovert 200

  • Page 1

    18 feb 2011, p. 1/8 q uick s tart g uide : w idefield 1 z eiss a xiovert 200 / v olocity (safb 409) startup procedure ................................................................................................................ 2 adjusting brightfield köhler illumination ............................

  • Page 2: Startup Procedure

    18 feb 2011, p. 2/8 startup procedure turn on (in this order): • if required: heater unit and heater, 4 hours before live imaging to allow focus to stabilise: • c plate holder • d chamber • if required: co 2 supply • switch on the mercury fluorescence lamp • main switch on the wall, on the right sid...

  • Page 3: Acquiring Images With The

    18 feb 2011, p. 3/8 observing fluorescence through the eyepiece there are 2 options to observe fluorescence: the common way would be to use the filter cubes in the cube revolver; for some applications the use of a dichroic mirror in the filter cube position together with the filters in the filter wh...

  • Page 4

    18 feb 2011, p. 4/8 o refocus/ re-centre or o choose a flourescent preset o if there is only a faint signal switch "auto contrast" on o refocus/ re-centre o switch off "auto contrast" adjusting channel presets: o choose a preset button for your channel settings by clicking on one o standard presets ...

  • Page 5: Shutdown Procedure

    18 feb 2011, p. 5/8 ƒ in "acquisition setup" dialogue • set "change focus using" to "zeiss focus drive" • choose and set z-spacing or number of slices • set "auto-focus using" to "zeiss focus drive" • choose the lightpath where the autofocus should take its cues from o set time series o in "acquisit...

  • Page 6

    18 feb 2011, p. 6/8 if someone’s booked within two hours: • update usage in sharepoint • remove your samples • clean objective lenses with fresh lens tissue and close incubation chamber • clear up the desk • save files onto the server or on a mobile harddrive • log off.

  • Page 7

    18 feb 2011, p. 7/8 important additional information volocity video toolbar.

  • Page 8

    18 feb 2011, p. 8/8 auto exposure • sets max pixels to 80% (not 100%) (can also be applied to a selected area) auto contrast • unlike gain only affects appearance of the image on the screen(!!!) keypad controls: • top level button 1 = transmitted light on/off (#2-4 = empty) • fw button switches betw...