Zeiss AxioObserver Quick Start Manual - page 12
RCF Microscopy Manual
5/2/2017
Authors Cory Nook and Chaowei Shang
Page 12 of 19
You will see the next screen to adjust different
channels. To add a channel, click on the
numbered icon that has an X through it at the
top of the window and right click on it with the
mouse. To remove a channel, right click on the
number that is surrounded with colour. Select
a channel by left clicking on the numbered
icon. Each channel allows you to define a dye,
colour, and name. The dye choices are given by
Zeiss and are mainly used for meta data.The
Hardware Settings section is what the
microscope changes for each channel to
acquire the image. For example, if the channel
definition is TRITC and you have the
Workgroup: DAPI selected in the During
acquisition part of the Hardware Settings, you
will get an image of dye excitation similar to
DAPI but will be labelled with TRITC. Select the
arrow the left of the Go icon and it will give
you a pull down menu of work group presets to
acquire images. Select the appropriate
Workgroup setting for the given dye. We
recommend that in the After acquisition
setting, that you turn off the corresponding
light source.
4.4 Setting Exposure
Switch from the eyes mode to the camera
mode to allow images to be shown on the
software. (Switch back from camera to eyes
when observing through the eyepieces.)
The Exposure section is used to capture the
images. The given time is how long the camera
will open the shutter and collect photons from
the specimen given the selected filter cube.
The time can be adjusted manually or by using
the measure button to the right. The measure