Zeiss LSM 510 Inverted User Manual

Other manuals for LSM 510 Inverted: Operating Manual, Operating Manual, User Manual
Manual is about: Laser Scanning Microscope

Summary of LSM 510 Inverted

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    1 use of the zeiss lsm 510 inverted firstly please be aware that this microscope should be treated with respect and care at all times. Rules of use:  this microscope can only be used by masters by research or phd students, postdocs and members of staff.  you must receive training from ann wheeler ...

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    2 the screen looks like this once you have switched everything on. The control menu is on the left and you work from top to bottom. Your images are stored on the right. Light path (eyes and laser is controlled here) 2. Turn on the lasers to turn on lasers select laser menu, rhs of workspace. Drop it...

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    3 3. Select the configuration you need pick imaging configuration from the drop down menu select the configuration you need click the icon to activate your chosen configuration 4. Set the microscope up and find your specimen click the ocular button to send the light to your eyes (top right area of t...

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    4 5. Start confocal scanning click lsm button (top right of the workspace) click the fast button (found on top left of the workspace) click split on the left hand side of your image (this splits the image according to channels used) 6 . Set the levels for each channel click the coloured region under...

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    5 7 .Resize your images using the crop tool. To stop scanning at any time click stop stop scanning and press crop (bottom of screen) simply put the cropping window (red box with cross hair) over the region you want to crop change the position, size or rotation of the cropped area by dragging the edg...

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    6 9. Take the picture click single in the scan menu 10. Saving your image(s) go to the open images section on the top right of the workspace select the one that you want to save click the floppy disk icon to save the image (please save to your folder in users on e‐drive!!) to convert to tif go to th...

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    7 12. To take a z stack go to the z‐stack toolbar (found on the left side of the workspace) and drop it down click on the tick to activate the z stack menu (you can't collect a z stack without this) its white when activated choose if you want to select first and last planes or to collect data from a...