Zeiss LSM 510 Brief Operating Manual - page 13

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13 

Image optimization 

Choosing a lookup table 

•  Select  Palette in the Image window of the 

scanned image (Fig. 19). 

The Color Palette window appears. 

•  In the Color Palette List panel, click on the 

Range Indicator item (Fig. 20). 

The scanned image appears in a false-color 

presentation (Fig. 19). 

If the image is too bright, it appears red on the 

screen. Red = saturation (maximum). 

If the image is not bright enough, it appears blue 

on the screen. Blue = zero (minimum). 

Adjusting the laser intensity 

•  Set the Pinhole to 1 Airy Unit (Fig. 21). 

•  Set the Detector Gain high. 

•  When the image is saturated, reduce AOTF 

transmission in the Excitation panel (Fig. 21) 

using the slider to reduce the intensity of the 

laser light at the specimen. 

Adjusting gain and offset 

•  Increase the Amplifier Offset until all blue 

pixels disappear, and then make it slightly 

positive (Fig. 21). 

•  Reduce the Detector Gain until the red pixels 

only just disappear. 

Fig. 19 

Image window 

Fig. 20 

Color Palette window 

Fig. 21 

Scan Control window, Channel settings