Zeiss LSM 510 Brief Operating Manual - page 7

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Selecting an objective 

•  Open the graphical pop-up menu by clicking on 

the Objective button (Fig. 10). 

•  Click on the objective you want to select. The 

selected objective will automatically move into 

the beam path. 

Focussing the microscope for transmitted light 

•  Open the graphical pop-up menu by clicking on 

the Transmitted Light button (Fig. 11). 

•  Click on the On button. Set the intensity of the 

Halogen illuminator using the slider. 

•  Click on Close to close the pop-up menu. 

•  Place specimen on microscope stage. The cover 

slip must be facing up. 

•  Use the focusing drive of the microscope to 

focus the required object plane. 

•  Select specimen detail by moving the stage in X 

and Y using the XY stage fine motion control. 

Setting the microscope for reflected light 

•  Click on the Reflected Light button to open 

the shutter of the HBO 100 mercury lamp. 

•  Click on the Reflector button and select the 

desired filter set by clicking on it. 

Storing the microscope settings 

Microscope settings can be stored and up to 8 

buttons assigned for fast retrieval and adjustment 

using the Microscope Settings panel. 

The  Store button permits existing microscope 

configurations to be stored under any name. 

The  Apply button permits existing stored 

microscope configurations to be loaded. 

The  Delete button permits existing microscope 

configurations to be deleted. 

The  Assign button permits the assignment of a 

microscope configuration to a button. 

Note: 

Depending on the microscope 

configuration, settings must be done manually if 

necessary. 

Fig. 10 

Microscope Control window, 

e.g.: Axiovert 200 M 

Fig. 11 

Microscope Control window with 

Transmitted Light pop-up menu