Zeiss LSM 510 Brief Operating Manual - Setting The Microscope

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•  Click on the Laser button to open the Laser Control window. 

•  Select the appropriate Laser Unit by clicking on 

the name of it. 

•  Click on the Standby button to switch required 

laser(s) to Standby.  

•  When status is Ready click on On button. 

•  Set  Output [%] so that the Tube Current is 

about 4 A (50% of the output power). 

Setting the microscope 

Changing between direct observation or laser scanning 

The VIS, TV and LSM buttons switch the beam path and indicate which beam path has been set in the 

binocular tube of the microscope: 

•  Click on the VIS button to set the microscope for direct observation via the eyepieces 

of the binocular tube, lasers are off. 

•  Click on the TV button to set the microscope camera observation (if connected) via 

camera adapter of the binocular tube. 

•  Click on the LSM button to set the microscope screen observation via laser excitation 

using the LSM 510 and software evaluation. 

Setting the microscope and storing the settings 

•  Click on the VIS button for direct observation. 

•  Click on the Micro  button in the Acquire subordinate toolbar to open the 

Microscope Control window of the used microscope. 

The Microscope Control window appears (Fig. 10). 

Fig. 9 

Laser Control window