Odyssey LI-COR Operator's Manual - Using The Mousepod Accessory

21

•

Protect plates from light before imaging to ensure highest sensitivity. When 

storing plates after imaging, protect plates from light at room temperature.

•

A 

Focus Offset

 of 3.0 mm should be entered in the scan parameters 

(Chapter 2, 

Odyssey® User Guide

) when using the plates specified above.

Plates other than those recommended above may require lower or higher 

focus offsets for optimal resolution and detection. If alternative plates are 

used, an initial optimization scan will be necessary. Scan a plate 

containing experimental and control samples at 0.5, 1.0, 2.0, 3.0, and 4.0 

mm focus offsets. Use the same intensity settings for each scan. After 

reviewing the collected scans, use the focus offset with the highest signal 

to noise as the focus offset for experiments with the alternate plates.

•

The 

Intensity

 scan parameter for both 700 and 800 nm channels should be 

set to 5 for initial scanning. If the image signal is saturated or too high, re-

scan using a lower intensity setting (i.e. 2.5). Likewise, If the image signal 

is too low, re-scan using a higher intensity setting (i.e. 7.5).

•

For satisfactory images with minimal scan time, the 

Quality

 scan 

parameter should be medium, with 

Resolution

 set to 169 µm. Higher scan 

quality and resolution may be used, but scan time will increase.

Using the MousePOD Accessory

Figure 3-1

. The MousePOD Accessory.

The optional Odyssey 

MousePOD

®

 Accessory fits 

over the Odyssey scanning 

surface for 

in vivo

 imaging of 

up to three mice in a temper-

ature-controlled enclosure. 

Operation of the MousePOD 

Accessory is described in the 

Odyssey 

In vivo

 Imaging 

Guide available at: 

http://biosupport.licor.com