Odyssey LI-COR Operator's Manual - Editing The Scan Parameters
CHAPTER 7
84
Starting Scans Using An Internet Browser
Editing the Scan Parameters
4)
Customize the Preset parameters by editing individual scan
parameters using the scan parameter definitions given below.
Resolution
can be set to 21, 42, 84, 169, or 337 µm. An option
named “Preview” is also available for quick low resolution preview
scans. For typical scans of membranes or gels, 169 µm scans should
suffice. Resolution can be increased as needed, however, note that
the file sizes can get very large. If the images are to be analyzed with
the Odyssey
®
Application Software, choose a resolution that keeps
the file size of individual images under 10 MB (20 MB for both
images).
Quality
controls scanning speed and ultimately how many detector
readings are processed for a given area on the membrane to make a
pixel on the image. For typical scans,
Medium
is recommended, but
there are five settings. Choosing
Highest
quality will reduce noise in
the image data, but significantly increase scanning time due to the
slower scanning speed. Similarly, choosing
Lowest
will decrease
scanning time, but increase noise in the image data. For high
resolution scans where samples have very little fluorescence,
High
or
Highest
may be a better choice than
Medium
. When quality is set too
low the image will become noisy or "grainy", particularly in the
background.
The
Intensity
fields control the detector sensitivity and affect the
band intensity on the image. If the intensity is set too high, the
detector may saturate, which produces white areas in the middle of
intense bands/dots on color images. If the intensity is set too low, the
image may not show any signal even though there is adequate
fluorescence from the samples. LI-COR Presets use an intensity value
of 5.0 for membranes, 8.0 for DNA gels, 5.0 for protein gels, and 5.0
for microplates. These settings may need to be optimized for your
scans due to the differing background fluorescence of various